Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,405,946 A→G 15.0% G232G (GGT→GGC lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,405,9460AG15.0% 272.0 / 41.3 120G232G (GGT→GGClrhAtranscriptional repressor of flagellar, motility and chemotaxis genes
Reads supporting (aligned to +/- strand):  ref base A (55/47);  new base G (14/4);  total (69/51)
Fisher's exact test for biased strand distribution p-value = 7.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-03

Only 100 of 120 total aligned reads displayed.

AAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATA  >  NC_000913/2405848‑2406042
                                                                                                  |                                                                                                
aaaTATTCGGTATCGGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGAc                                                                                                 <  1:1528215/100‑1 (MQ=255)
aaaTATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGAc                                                                                                 >  1:2627746/1‑100 (MQ=255)
 aaTATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGAcc                                                                                                <  2:459280/100‑1 (MQ=255)
    atTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGTCTTGGCGTGACGGCAAGGCCggt                                                                                             <  2:1224073/100‑2 (MQ=37)
     ttCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGc                                                                                             <  1:2093067/99‑1 (MQ=255)
       cGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCttt                                                                                          >  2:1258639/1‑100 (MQ=255)
       cGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGCCCGCGCTCATCATCTCACCCGGCCTTGCCGTCACGCCAACACCGGCttt                                                                                          <  1:2598926/100‑1 (MQ=255)
       cGGTAGCAGGTAGCGGAGGTAAGCCATCGACGCCACTCAGCACACGCAGGCCCGGGCTTATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGc                                                                                             <  1:945673/97‑1 (MQ=255)
        ggTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTc                                                                                         <  2:2571334/100‑1 (MQ=255)
        ggTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTc                                                                                         >  1:2033933/1‑100 (MQ=255)
        ggTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTc                                                                                         <  1:1600099/100‑1 (MQ=255)
          tATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGTCTTGGCGTGACGGCAAGGCCggttgagat                                                                                       <  2:165406/100‑8 (MQ=25)
            tCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTg                                                                                     >  2:1539166/1‑100 (MQ=255)
              aGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGcc                                                                                   <  1:544793/100‑1 (MQ=255)
               ggTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCACCCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGc                                                                                    <  1:2919794/98‑1 (MQ=255)
               ggTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCg                                                                                  <  1:2915179/100‑1 (MQ=255)
                gTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCCCCACCGGCATCGCCGTCACGCCAAGACCGGCTTTCACTGCCGc                                                                                 >  1:2332858/1‑100 (MQ=255)
                 tAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCa                                                                                >  2:2291687/1‑100 (MQ=255)
                 tAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCa                                                                                >  2:2914079/1‑100 (MQ=255)
                 tAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCa                                                                                <  1:1171339/100‑1 (MQ=255)
                  aGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCAc                                                                               <  1:1148369/100‑1 (MQ=255)
                     ggcggTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGaa                                                                            >  1:610000/1‑100 (MQ=255)
                     ggcggTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGaa                                                                            >  2:2805863/1‑100 (MQ=255)
                     ggcggTAAGCCATCTACTCCACTCAGCACACGAAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGaa                                                                            >  2:106005/1‑100 (MQ=255)
                       cggTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACg                                                                          <  1:396980/100‑1 (MQ=255)
                         gTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGc                                                                        >  2:637452/1‑100 (MQ=255)
                            aGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGGCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCgg                                                                     <  1:1814010/100‑1 (MQ=255)
                               cATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAg                                                                  >  1:168223/1‑100 (MQ=255)
                                 tCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCg                                                                <  2:677532/100‑1 (MQ=255)
                                  cTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGt                                                               >  2:1811257/1‑100 (MQ=255)
                                   tACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTc                                                              <  2:2325870/100‑1 (MQ=255)
                                    aCTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCg                                                             >  1:621633/1‑100 (MQ=255)
                                     cTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGa                                                            >  1:34326/1‑100 (MQ=255)
                                       ccACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCCCTGCCGCACGAACGGCCGGAAGCGTCGAgg                                                          >  2:1318122/1‑100 (MQ=255)
                                       ccACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAgg                                                          >  2:1005846/1‑100 (MQ=255)
                                       ccACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAgg                                                          >  2:2232677/1‑100 (MQ=255)
                                       ccACTCAGCACACGCAGATCAGGACTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCCGTCCCTGCCGCACGAACGGCCGGAAGCGTCGAgg                                                          >  1:784902/1‑100 (MQ=255)
                                        cACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACACCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGc                                                         >  2:965631/1‑100 (MQ=255)
                                         aCTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGcn                                                        <  2:1832764/100‑2 (MQ=255)
                                          cTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGc                                                         >  1:2503619/1‑98 (MQ=255)
                                           tCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACTGGCTTTCACTGCCGCACGAACGGCCGGACGCGTCGAGGCgcc                                                      <  2:1974771/100‑3 (MQ=255)
                                            cAGCACACGCATGTCCGGGCTCATCATCTCAACCGGCCTTGCAGACACGCCAGGACCTGCTTTCACTGCCGCACGACCGGCCGGCAGCgcccacgcg                                                        >  1:466479/1‑89 (MQ=255)
                                            cAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACa                                                     <  1:2341936/100‑1 (MQ=255)
                                                 cacGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGc                                                >  2:1343071/1‑100 (MQ=255)
                                                 cacGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGc                                                <  2:1675978/100‑1 (MQ=255)
                                                  acGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGcc                                               <  1:1166138/100‑1 (MQ=255)
                                                  acGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGcc                                               <  1:377165/100‑1 (MQ=255)
                                                   cGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCa                                              >  1:2628845/1‑100 (MQ=255)
                                                   cGCAGGTCCGGGCTCATCATCTCAACCGGCCTTCCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAAAGGCCGGAAGCGTCGAGGCGACATAAGCCa                                              >  1:1749344/1‑100 (MQ=255)
                                                     cAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGt                                            >  1:2877007/1‑100 (MQ=255)
                                                         tCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGcc                                        >  1:569540/1‑100 (MQ=255)
                                                          ccGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCa                                       >  1:2302483/1‑100 (MQ=255)
                                                            gggCTCATCATCTCAACCGGCCTTGCCGTGACGCCAAGACCGGCTTTCGCTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATg                                     <  1:1286200/100‑1 (MQ=255)
                                                            gggCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATg                                     <  2:2319364/100‑1 (MQ=255)
                                                            gggCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATg                                     >  2:347951/1‑100 (MQ=255)
                                                             ggCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATgg                                    >  1:2864905/1‑100 (MQ=255)
                                                                tcatcaTCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAat                                 <  1:637449/100‑1 (MQ=255)
                                                                    catcTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGTCAGTCGCCATGGAATATCt                             <  2:2336560/100‑1 (MQ=255)
                                                                    catcTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCt                             >  2:516538/1‑100 (MQ=255)
                                                                    catcTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCt                             <  1:2869959/100‑1 (MQ=255)
                                                                    agtgaaagccGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCt                             >  1:2713710/9‑100 (MQ=25)
                                                                     atcTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTg                            >  2:1263088/1‑100 (MQ=255)
                                                                     atcTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACAAAAGCCAGTCGCCCTGGAATCTCTg                            >  1:1809091/1‑100 (MQ=255)
                                                                      tctcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGc                           >  1:895820/1‑100 (MQ=255)
                                                                       gaaagccGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCt                          >  1:195514/6‑100 (MQ=25)
                                                                       ctcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCt                          <  1:389336/100‑1 (MQ=255)
                                                                       ctcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCt                          >  2:2133571/1‑100 (MQ=255)
                                                                       ctcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGACGACATAAGCCAGTCGCCATGGAATATCTGCt                          >  1:1037336/1‑100 (MQ=255)
                                                                        tcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCtt                         <  1:2188207/100‑1 (MQ=255)
                                                                         cAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCttt                        <  1:1811249/100‑1 (MQ=255)
                                                                         aagccGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCttt                        >  2:237193/4‑100 (MQ=37)
                                                                          aaCCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTtta                       <  2:2453901/100‑1 (MQ=255)
                                                                           gccGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttat                      >  2:553675/2‑100 (MQ=37)
                                                                           gccGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCGttat                      >  2:751943/2‑100 (MQ=37)
                                                                           aCCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttat                      <  2:2107998/100‑1 (MQ=255)
                                                                            ccGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttatt                     >  2:124888/1‑100 (MQ=38)
                                                                            ccGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttatt                     >  2:698490/1‑100 (MQ=38)
                                                                            ccGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttatt                     >  2:2619581/1‑100 (MQ=38)
                                                                            ccGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttatt                     >  2:1116854/1‑100 (MQ=255)
                                                                            ccGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttatt                     <  1:706795/100‑1 (MQ=255)
                                                                              ggtcTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttataga                   >  2:337680/4‑97 (MQ=255)
                                                                              ggtcTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTaa                   >  2:1648261/4‑100 (MQ=255)
                                                                              ggtcTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTaa                   >  1:924485/4‑100 (MQ=255)
                                                                              ggtcTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTaa                   >  2:1647251/4‑100 (MQ=255)
                                                                              ggCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTaa                   >  1:995118/1‑100 (MQ=255)
                                                                              ggCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTaa                   >  2:2412120/1‑100 (MQ=255)
                                                                              ggCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGAACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttatta                    <  2:1624171/99‑1 (MQ=255)
                                                                               gCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAg                  >  2:2717189/1‑100 (MQ=255)
                                                                                ncTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGc                 >  1:1045704/2‑100 (MQ=255)
                                                                                ccTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGc                 >  2:1899375/1‑100 (MQ=255)
                                                                                 cTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCg                <  2:959014/100‑1 (MQ=255)
                                                                                   tGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAACCGTc              >  2:216949/1‑100 (MQ=255)
                                                                                     ncGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTGTATTAAGCGTCGc            >  1:1398621/2‑100 (MQ=255)
                                                                                     ccGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGTAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGc            <  1:2040075/100‑1 (MQ=255)
                                                                                      cGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGcc           <  1:2000187/100‑1 (MQ=255)
                                                                                      cGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGcc           <  1:1541171/100‑1 (MQ=255)
                                                                                        tCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAg         >  1:2198035/1‑100 (MQ=255)
                                                                                         cACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAACATCTGCTTTATTAAGCGTCGCCAGc        >  2:2548206/1‑100 (MQ=255)
                                                                                              cAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCat   >  2:680689/1‑100 (MQ=255)
                                                                                               aaGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCata  >  2:1569846/1‑100 (MQ=255)
                                                                                                  |                                                                                                
AAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATA  >  NC_000913/2405848‑2406042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: