BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,663,466	G→T	15.6%	V9L (GTG→TTG)	suhB →	inositol monophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,663,466	0	G	T	15.6%	320.4 / 38.9	113	V9L (GTG→TTG)	suhB	inositol monophosphatase
Reads supporting (aligned to +/- strand): ref base G (51/44); new base T (8/10); total (59/54)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACC  >  NC_000913/2663368‑2663564

cGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGt                                                                                                   >  2:178199/1‑100 (MQ=255)

  aaGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgc                                                                                                 >  1:100839/1‑100 (MQ=255)

   aGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgcg                                                                                                <  2:1400182/100‑1 (MQ=255)

      cAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgcgcgc                                                                                             >  1:857091/1‑100 (MQ=255)

          tGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgcgcgcagcg                                                                                         >  2:2363234/1‑100 (MQ=255)

            ccGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgcgcgcagcgcg                                                                                       <  1:2404484/100‑1 (MQ=255)

            ccGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgcgcgcagcgcg                                                                                       >  1:294787/1‑100 (MQ=255)

              gATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgcgcgcagcgcgca                                                                                     >  1:83211/1‑100 (MQ=255)

                  tcGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCTTGCGCGCTGCGCGCACGGc                                                                                 <  2:2155911/100‑1 (MQ=255)

                  tcGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGc                                                                                 >  2:217089/1‑100 (MQ=255)

                  tcGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGc                                                                                 >  2:1297529/1‑100 (MQ=255)

                   cGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGGACATCGCCGTGCGCGCAGCGCGCAAGGCg                                                                                >  2:2862612/1‑100 (MQ=255)

                    gctgctATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCgg                                                                               <  1:1105399/100‑1 (MQ=255)

                     ctgctATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCggg                                                                              <  1:1976626/100‑1 (MQ=255)

                     ctgctATACTCTGAGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCggg                                                                              >  2:1976637/1‑100 (MQ=255)

                       gctATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCTTGCGCGCTGCGCGCACGGCgatgt                                                                            <  1:2673958/100‑5 (MQ=38)

                        ctATACTCTGCGCCGTTTTCCCGTTCTTTAACATCGAGTGAGAGAGACATATGCATCCGATGCTGAACATCGCCTTGCGCGCTGCGCGCACGGCgat                                                                              <  2:705646/97‑3 (MQ=37)

                         tagaCTCTGCGCCGTTTGCCCGTTCTTGAACATCCAGTGAGAGAGACTGATGCATCCGATGCTGAGCATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaat                                                                          <  2:118122/97‑1 (MQ=255)

                          ataCTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCTTGCGCGCTGCGCGCACGGCgatgttca                                                                         <  1:2173940/100‑8 (MQ=37)

                          ataCTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatt                                                                         >  2:2000876/1‑100 (MQ=255)

                           taCTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCTTGCGCGCTGCGCGCACGGCgatgttcag                                                                        <  1:1025516/100‑9 (MQ=37)

                           taCTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCTTGCGCGCTGCGCGCACGGCgatgttcag                                                                        <  1:1117470/100‑9 (MQ=37)

                           taCTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCTTGCGCGCTGCGCGCACGGCgatgttcag                                                                        <  1:202294/100‑9 (MQ=37)

                           taCTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaattt                                                                        <  1:1571740/100‑1 (MQ=255)

                             ctctGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaa                                                                      >  2:655487/1‑100 (MQ=255)

                             ctctGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaa                                                                      <  2:15299/100‑1 (MQ=255)

                             ctctGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaa                                                                      >  1:335048/1‑100 (MQ=255)

                              tctGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaat                                                                     <  2:1941860/100‑1 (MQ=255)

                                tGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTg                                                                   <  2:1503595/100‑1 (MQ=255)

                                tGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTg                                                                   <  1:1297524/100‑1 (MQ=255)

                                tGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTg                                                                   <  1:1039806/100‑1 (MQ=255)

                                 gcgcCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGc                                                                  <  2:2607160/100‑1 (MQ=255)

                                   gcCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCa                                                                >  1:437212/1‑100 (MQ=255)

                                   gcCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCa                                                                >  1:1664354/1‑100 (MQ=255)

                                     cGTTTTCCCGTTCTTTAACATCCAGTGAGCGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCaa                                                               <  1:2230202/99‑1 (MQ=255)

                                      gTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCaaaa                                                             >  2:1779743/1‑100 (MQ=255)

                                      gTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCaaaa                                                             >  1:1758984/1‑100 (MQ=255)

                                        tttCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCAGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAAc                                                           >  2:1724966/1‑100 (MQ=255)

                                          tCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTa                                                         <  2:1032817/100‑1 (MQ=255)

                                           cccGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTAt                                                        >  1:1203087/1‑100 (MQ=255)

                                           cccGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTAt                                                        >  2:1219115/1‑100 (MQ=255)

                                            ccGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATg                                                       <  2:2642117/100‑1 (MQ=255)

                                            ccGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATg                                                       >  1:2341219/1‑100 (MQ=255)

                                            ccGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCACGCAAGGCGGGTAATTTAATTGCCAAAAACTATg                                                       >  1:1535777/1‑100 (MQ=255)

                                                 cTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTCCCAAAAACTATGaaaac                                                  >  1:1177135/1‑98 (MQ=255)

                                                  tttAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACCTCGCCGTGCGCACAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAAccc                                                 <  1:2044395/100‑1 (MQ=255)

                                                  tttAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAAccc                                                 >  2:1541371/1‑100 (MQ=255)

                                                  tttAACATCCAGAGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAAccc                                                 >  2:1920430/1‑100 (MQ=255)

                                                     aaCATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCgg                                              <  2:2438469/100‑1 (MQ=255)

                                                      aCATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGa                                             <  1:910684/100‑1 (MQ=255)

                                                       cATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGAc                                            <  1:1753021/100‑1 (MQ=255)

                                                          ccAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCt                                         <  1:2755932/100‑1 (MQ=255)

                                                           cAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTg                                        >  1:2551380/1‑100 (MQ=255)

                                                           cAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTg                                        <  1:76935/100‑1 (MQ=255)

                                                           cAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTg                                        >  1:445652/1‑100 (MQ=255)

                                                            aGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCTTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGt                                       <  1:2850891/100‑1 (MQ=255)

                                                            aGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGt                                       <  2:2356881/100‑1 (MQ=255)

                                                               gagagagaCCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGa                                    >  1:809177/1‑100 (MQ=255)

                                                                  agagaCCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGc                                 <  1:431296/100‑1 (MQ=255)

                                                                  agagaCCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGc                                 <  1:1945259/100‑1 (MQ=255)

                                                                   gagaCCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGCCGCTGTAGAAGCg                                >  1:872975/1‑100 (MQ=255)

                                                                     gaCCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAg                              <  1:623485/100‑1 (MQ=255)

                                                                      aCCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCACAAACTATGAAACCCCGGACGCTGTAGAAGCGa                               >  1:1723135/1‑98 (MQ=255)

                                                                      aCCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGc                             <  2:566605/100‑1 (MQ=255)

                                                                       ccGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGcc                            >  2:1397908/1‑100 (MQ=255)

                                                                         gATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAg                          >  2:1543926/1‑100 (MQ=255)

                                                                         gATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAg                          >  2:1265557/1‑100 (MQ=255)

                                                                         gATGAATCCGATGCTGAACATGGAAGTGTGTGCAGCGTGCAAGGCGGGTAATTTAATTGTGAAAAACTATGAAAACCCGGACGTTGTAGAGGGGAGCTAg                          >  1:54175/1‑100 (MQ=255)

                                                                           tGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGaa                        >  1:2534506/1‑100 (MQ=255)

                                                                           tGCATCCGACGCGGAATACCGCTGTGCGCGCAGCGCGCGAGGGGGGTAACCGAATGGCGAAACACGATGAAAACCCGGACACTGTACAAGCGTGCCa                           <  1:490401/97‑1 (MQ=255)

                                                                             cATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAg                      >  1:274972/1‑100 (MQ=255)

                                                                               tCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGCCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGc                    >  1:1553958/1‑100 (MQ=255)

                                                                                ccGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCa                   >  2:2576794/1‑100 (MQ=255)

                                                                                ccGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCa                   <  2:2411330/100‑1 (MQ=255)

                                                                                 cGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAg                  <  2:2321911/100‑1 (MQ=255)

                                                                                 cGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAg                  >  1:1787964/1‑100 (MQ=255)

                                                                                  gaggCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAg                  <  1:2461429/96‑1 (MQ=255)

                                                                                   aTGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTa                <  2:1248041/100‑1 (MQ=255)

                                                                                     gCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAc              <  2:2112743/100‑1 (MQ=255)

                                                                                     gCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAc              >  1:794753/1‑100 (MQ=255)

                                                                                       tGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGa            >  2:704565/1‑100 (MQ=255)

                                                                                       aattacccGCCTTGCGCGCTGCGCGCAAGGCGGGTAATTTAAATGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGa            >  2:1972652/8‑100 (MQ=38)

                                                                                        gAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGa            >  1:2177153/1‑99 (MQ=255)

                                                                                          aCATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGAttt         <  2:100840/100‑1 (MQ=255)

                                                                                           cATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTc        >  2:2740993/1‑100 (MQ=255)

                                                                                            cccGCCTTGCGCGCTGCGCGCACGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCg       >  2:2375243/3‑100 (MQ=38)

                                                                                            cccGCCTTGCGCGCTGCGCGCACGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCg       >  2:804326/3‑100 (MQ=38)

                                                                                            cccGCCTTGCGCGCTGCGCGCACGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCg       >  2:2336537/3‑100 (MQ=38)

                                                                                            cccGCCTTGCGCGCTGCGCGCACGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCg       >  2:671277/3‑100 (MQ=38)

                                                                                            aTCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCg       <  1:800616/100‑1 (MQ=255)

                                                                                             tCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGt      <  2:2363621/100‑1 (MQ=255)

                                                                                             ccGCCTTGCGCGCTGCGCGCACGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGt      >  2:780556/2‑100 (MQ=39)

                                                                                              cGCCTTGCGCGCTGCGCGCACGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGAAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTg     >  2:2319276/1‑100 (MQ=255)

                                                                                              cGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTg     <  2:2341234/100‑1 (MQ=255)

                                                                                               gCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGa    >  2:2306048/1‑100 (MQ=255)

                                                                                                ccGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGAc   <  2:2821664/100‑1 (MQ=255)

                                                                                                ccGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGAc   <  2:1553964/100‑1 (MQ=255)

                                                                                                ccGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGAc   <  2:726627/100‑1 (MQ=255)

                                                                                                 cGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGAcc  <  2:2868257/100‑1 (MQ=255)

                                                                                                 cGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGAcc  >  2:2092099/1‑100 (MQ=255)

CGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACC  >  NC_000913/2663368‑2663564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`