| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,731,312 | G→A | 70.1% | intergenic (‑155/+288) | rrsG ← / ← clpB | 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,731,312 | 0 | G | A | 70.1% | 86.2 / 58.7 | 80 | intergenic (‑155/+288) | rrsG/clpB | 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base G (21/2); new base A (28/27); total (49/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.95e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
GTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCC > NC_000913/2731220‑2731408 | gtcttCATTTTTGGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCGCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCttt < 2:1153938/94‑1 (MQ=0) ctcctgagaactccggcagagacagcaaaattacatgctccactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAccgg > 1:412052‑M1/78‑100 (MQ=255) ctcctgagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaagcgtattatgcacaccccgcGCCGCTTTGCTTTTTCTCAgcgg > 2:1536445‑M1/78‑100 (MQ=255) ttgtCGTCTTGGGAGGTTAAGAAGTCGTAGTTTCGAGTTCGCACACATATTGTCTGATATCTTGTTAAAGAGCAGGGCCGCTGCTCCTTTTCTcggcgg < 1:2875174/96‑1 (MQ=0) cctgagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaagcgtattatgcacaccccgcGCCGCTTTGCTTTTTCTCAgcggcg > 2:2093503‑M1/76‑100 (MQ=255) ctgagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGgcgc > 1:1155706‑M1/75‑100 (MQ=255) ctgagaactccggcagagaaagcaaaaataaaggcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGgcgc > 2:1206072‑M1/75‑100 (MQ=255) tgagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGcgcg > 2:399683‑M1/74‑100 (MQ=255) tgagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGcgcg > 2:2825682‑M1/74‑100 (MQ=255) tgagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaagcgtattatgcacaccccgcGCCGCTTTGCTTTTTCTCAGCGGcgcg > 2:2160448‑M1/74‑100 (MQ=255) tcaaaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggtaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGcgcg > 1:1764925‑M1/74‑100 (MQ=255) gagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGcgcg > 1:2565837‑M1/73‑99 (MQ=255) gagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCgg > 2:125985‑M1/73‑100 (MQ=255) gagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCgg > 2:1300516‑M1/73‑100 (MQ=255) agaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCggg > 1:465212‑M1/72‑100 (MQ=255) gaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAgcggcg > 1:2066952‑M1/71‑95 (MQ=255) gaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCgggg > 2:2796782‑M1/71‑100 (MQ=255) gaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCgggg > 2:297563‑M1/71‑100 (MQ=255) gaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaagcgtattatgcacaccccgcGCCGCTTTGCTTTTTCTCAGCGGCGCgggg > 2:2407603‑M1/71‑100 (MQ=255) agagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCataat > 1:524322‑M1/61‑100 (MQ=255) agaaaagcaaagaaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACg > 2:1779852‑M1/58‑100 (MQ=255) aaagaaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCataata > 1:1022289‑M1/57‑97 (MQ=255) aataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGc > 2:907429‑M1/49‑100 (MQ=255) ttGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCttt < 1:517971/100‑1 (MQ=17) tGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg < 2:2520720/100‑1 (MQ=18) tGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg < 1:874716/100‑1 (MQ=18) gTCTGATACATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc < 1:1734580/100‑1 (MQ=255) gTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTTTTTTTTTTTCAGGGCGTGCATAATACGCCTTCTCTCTACAGAGTCAAGCATTTCTTTg > 1:2222712/1‑99 (MQ=2) gTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc < 1:2806362/100‑1 (MQ=25) gTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc > 1:1884721/1‑100 (MQ=25) tCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCt < 1:121128/100‑1 (MQ=38) tCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCt > 2:1319461/1‑100 (MQ=255) tCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCt > 2:2706216/1‑100 (MQ=255) cTGATAAATTGTTCAAGAGCAGTGCCGCTTCACTTTTTGTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt > 1:1796893/1‑100 (MQ=255) cTGATAAATTGTTAATTAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTAAAGCATTTCTTTGCtt > 1:630189/1‑100 (MQ=14) cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTTTTTTTTTTTTTTGGTGTGAATAATACGCCTTCCCGTTTCAGAGTTAAGCATTTCTTTGCtt > 1:960788/1‑100 (MQ=2) cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTTTTTTCTTTGCTGAGTGTGCATAATACGCCTTCTTGCTACAATGGCAAGGATTTCTTTGCt > 1:630392/1‑99 (MQ=2) cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt > 1:279510/1‑100 (MQ=255) cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt > 2:518575/1‑100 (MQ=255) cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAACACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt < 2:1071866/100‑1 (MQ=255) tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt > 1:895302/1‑100 (MQ=255) tGATAAATTGATAAAGAGAAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGAGTGCATAATACGCCTTCACGCTACAGAGTCACGCATCTCTTTCCttt > 1:513964/1‑100 (MQ=11) gATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTTTTTTTTTTTTGGGGTGTGCATAATACGCCTTCCCTCTACATAGTCAAGCATTTCTTTGCtttt > 1:635361/1‑100 (MQ=9) gATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt > 2:646033/1‑100 (MQ=255) gATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt > 2:22343/1‑100 (MQ=255) gATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt > 1:1189053/1‑99 (MQ=255) tAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCCTAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt > 2:997442/1‑98 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc < 2:279511/100‑1 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc < 1:1962001/100‑1 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc < 1:2109051/100‑1 (MQ=255) aTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg > 2:1521622/1‑100 (MQ=255) aTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg < 1:1676902/100‑1 (MQ=255) ttGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGt < 2:739002/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 2:1689193/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg > 2:2624993/1‑100 (MQ=255) gTTAAAGAGCAGCGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 1:1156685/100‑1 (MQ=255) ttAATGAGGAGTGCCGCTTCTCTTTTTCTCAGCGGCGCGGGGTGTGCATGGTACGGCTGCTCGTTACAGAGTCAAGCATTGCTTTGCTTTTCTCTGTTga < 1:120515/100‑1 (MQ=17) ttAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg > 2:1137776/1‑99 (MQ=255) ttAAAGAGCAGTGCCGCTTCACTTTTTTTTTTTTTCTCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtct > 1:1724223/1‑93 (MQ=14) tAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag > 2:2080747/1‑100 (MQ=255) aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga > 1:1078825/1‑100 (MQ=255) aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga < 2:2606611/100‑1 (MQ=255) aaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCTGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt < 1:960849/100‑1 (MQ=255) aaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGTCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga < 1:505991/99‑1 (MQ=255) aaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt > 1:622806/1‑100 (MQ=255) gagCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtc < 2:2017946/100‑1 (MQ=255) agCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtct > 1:835718/1‑100 (MQ=255) agCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtct < 2:366876/100‑1 (MQ=255) gCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc < 2:783905/100‑1 (MQ=255) gCAGTGCCGCTTCACTTTTTCTCAGAGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc > 1:1209369/1‑100 (MQ=255) cAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa < 2:682635/100‑1 (MQ=255) aGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg < 1:2379424/100‑1 (MQ=255) tGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga < 2:1832231/100‑1 (MQ=255) GCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg > 1:140062‑M1/1‑97 (MQ=255) GCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag < 2:2643145‑M1/100‑1 (MQ=255) CCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga < 2:2377308‑M1/100‑1 (MQ=255) GCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc < 1:1451911‑M1/100‑1 (MQ=255) GCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc < 2:1511648‑M1/100‑1 (MQ=255) CTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcc > 2:2387910‑M1/1‑100 (MQ=255) TTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGCGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAccc < 2:255311‑M1/100‑1 (MQ=255) | GTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCC > NC_000913/2731220‑2731408 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |