Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,732,147 A→T 16.3% L676Q (CTG→CAG)  clpB ← protein disaggregation chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,732,1470AT16.3% 284.8 / 43.2 113L676Q (CTG→CAG) clpBprotein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base A (59/35);  new base T (10/8);  total (70/43)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 117 total aligned reads displayed.

GTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGA  >  NC_000913/2732050‑2732244
                                                                                                 |                                                                                                 
gTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCcagc                                                                                                 <  1:304261/100‑1 (MQ=255)
gTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCcagc                                                                                                 <  1:2591913/100‑1 (MQ=255)
  cGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCcagcag                                                                                               <  2:467317/100‑1 (MQ=255)
  cGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCcagcag                                                                                               >  2:1402764/1‑100 (MQ=255)
   gACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAgg                                                                                              >  2:46538/1‑100 (MQ=255)
    aCCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGa                                                                                             <  1:1497448/100‑1 (MQ=255)
    aCCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGa                                                                                             <  2:2782501/100‑1 (MQ=255)
     ccGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGAt                                                                                            >  2:1495008/1‑100 (MQ=255)
       gTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGa                                                                                          >  2:1281292/1‑100 (MQ=255)
       gTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGACGCGCTTCTTCCACTTCATCCAGCAGGATGa                                                                                          >  1:689769/1‑100 (MQ=255)
        ttCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGAc                                                                                         >  2:743662/1‑100 (MQ=255)
         tctcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACg                                                                                        <  2:2556842/100‑1 (MQ=255)
          ctcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCTGCTGGATGaagt                                                                                       <  1:245433/100‑4 (MQ=255)
          ctcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCTGCTGGATGaagt                                                                                       <  2:1711674/100‑4 (MQ=255)
          ctcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACgg                                                                                       >  1:617185/1‑100 (MQ=255)
           tcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCTGCTGGATGaagtg                                                                                      <  1:1103684/100‑5 (MQ=255)
           tcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCTGCTGGATGaagtg                                                                                      <  2:1931892/100‑5 (MQ=255)
           tcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGa                                                                                      >  2:1638422/1‑100 (MQ=255)
           tcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGa                                                                                      >  2:2566672/1‑100 (MQ=255)
           tcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTGGAAGACATCCGGATGCGCTTTTTCCACTTCATCCTGCTGGATGaagtg                                                                                      <  2:2683127/100‑5 (MQ=255)
           tcCCGTGCCCGTCAGTCAGACGCCCATCATCCAGTTCCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCTGCTGGATGaagtg                                                                                      <  2:1207295/100‑5 (MQ=255)
            cccTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCTGCTGGATGaagtgg                                                                                     <  1:1314188/100‑6 (MQ=255)
            cccTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCTGCTGGATGaagtgg                                                                                     <  1:1706022/100‑6 (MQ=255)
            cccTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGAGGCGCTTTTTCCACTTCATCCAGCAGGATGACGGaa                                                                                     >  2:642777/1‑100 (MQ=255)
                tGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACg                                                                                 >  2:1309735/1‑100 (MQ=255)
                  cccGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGga                                                                               >  2:231680/1‑100 (MQ=255)
                    cGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGgacg                                                                             <  1:2911874/100‑1 (MQ=255)
                       cagtcagACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCATGATGACGGAATACGgacgacg                                                                          <  1:528158/100‑1 (MQ=255)
                        agtcagACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGgacgacga                                                                         >  1:2452178/1‑100 (MQ=255)
                         gtcagACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGgacgacgac                                                                        >  1:2416948/1‑100 (MQ=255)
                           cagACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGc                                                                      >  2:233858/1‑100 (MQ=255)
                              aCGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGacgccgacg                                                                       >  1:2354075/1‑96 (MQ=255)
                              aCGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCAcc                                                                   <  2:314239/100‑1 (MQ=255)
                                  ccATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGc                                                                 >  1:678007/1‑98 (MQ=255)
                                  ccATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCtt                                                               >  2:2094645/1‑100 (MQ=255)
                                   catcatCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTc                                                              <  1:2304529/100‑1 (MQ=255)
                                   catcatCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTc                                                              <  1:46537/100‑1 (MQ=255)
                                     tcatcCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCgg                                                            <  2:1288548/100‑1 (MQ=255)
                                     tcatcCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCgg                                                            <  2:1288668/100‑1 (MQ=255)
                                     tcatcCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCgg                                                            <  1:1391307/100‑1 (MQ=255)
                                      catcCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGCTGACGGAATACGGACGACGACGCACCGCTTCGGt                                                           >  2:1247977/1‑100 (MQ=255)
                                       atcCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTc                                                          >  2:784975/1‑100 (MQ=255)
                                         ccAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAg                                                        <  2:2312322/100‑1 (MQ=255)
                                          cAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAgg                                                       >  2:1539809/1‑100 (MQ=255)
                                               ccTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGcc                                                  <  1:1495002/100‑1 (MQ=255)
                                                cTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCa                                                 <  1:2534296/100‑1 (MQ=255)
                                                 tGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCAc                                                >  1:2261203/1‑100 (MQ=255)
                                                 tGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCAc                                                >  2:1718175/1‑100 (MQ=255)
                                                  gCAACAGCATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCAcc                                               <  1:2505510/100‑1 (MQ=255)
                                                  gCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCAcc                                               >  2:78874/1‑100 (MQ=255)
                                                  gCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCAcc                                               >  2:2599705/1‑100 (MQ=255)
                                                     aCAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACcttc                                            >  2:1452783/1‑100 (MQ=255)
                                                      cAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGAGGGAATAGGGACGACGACGCACCGCTTCGGTCAGGTAGCCACcttct                                           >  2:2907091/1‑100 (MQ=255)
                                                        gAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCttcttc                                         >  1:2837336/1‑100 (MQ=255)
                                                         aaTGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCa                                        <  2:485665/100‑1 (MQ=255)
                                                           tGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATa                                      >  2:490955/1‑100 (MQ=255)
                                                            gTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACACACCGCTTAGGTCAGGTAGCCAACTTATTCATaa                                     >  2:16309/1‑100 (MQ=255)
                                                             ttGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAAc                                    >  1:528475/1‑100 (MQ=255)
                                                             ttGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACACACCGCTTCGGTCAGGTCGCCACCTTCTTCATCAc                                    >  1:2519084/1‑100 (MQ=255)
                                                              ttaaGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAAcc                                   <  1:2007173/98‑1 (MQ=255)
                                                              tGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAAcc                                   >  2:1577015/1‑100 (MQ=255)
                                                              tGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAAcc                                   >  2:298666/1‑100 (MQ=255)
                                                              tGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAAcc                                   <  1:443145/100‑1 (MQ=255)
                                                                 aGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGAc                                <  2:2657340/100‑1 (MQ=255)
                                                                 aGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGAc                                <  2:2358276/100‑1 (MQ=255)
                                                                  gACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACa                               <  1:1601346/100‑1 (MQ=255)
                                                                  gACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACa                               <  2:749130/100‑1 (MQ=255)
                                                                   aCATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACTGACTACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACa                               <  1:302141/99‑1 (MQ=255)
                                                                     aTCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGGCGAGGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACatat                            <  1:313619/100‑1 (MQ=255)
                                                                     aTCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACatat                            >  2:1320730/1‑100 (MQ=255)
                                                                     aTCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACatat                            >  1:677991/1‑100 (MQ=255)
                                                                         ggATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCg                        <  2:2557399/100‑1 (MQ=255)
                                                                          gATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCgg                       <  2:1484642/100‑1 (MQ=255)
                                                                            tGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAg                     <  2:832161/100‑1 (MQ=255)
                                                                               gcTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGgcg                  >  2:1049076/1‑100 (MQ=255)
                                                                               gcTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGgcg                  >  2:2607463/1‑100 (MQ=255)
                                                                                cTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGgcgc                 <  1:996085/100‑1 (MQ=255)
                                                                                 tttttCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCa                >  2:2247488/1‑100 (MQ=255)
                                                                                    ttCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCacca             >  1:95080/1‑100 (MQ=255)
                                                                                    ttCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCacca             >  1:255339/1‑100 (MQ=255)
                                                                                    ttCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCacc              <  2:1727505/99‑1 (MQ=255)
                                                                                     tCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCaccaa            >  2:1839630/1‑100 (MQ=255)
                                                                                      ttccgtCATCCCGCTGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCaccaac           >  1:1078291/6‑100 (MQ=255)
                                                                                      ccACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCaccaac           <  2:2851216/100‑1 (MQ=255)
                                                                                      ccACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCaccaac           >  2:2027637/1‑100 (MQ=255)
                                                                                       tccgtCATCCTGCTGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCaccaacc          >  1:2056986/5‑100 (MQ=255)
                                                                                       tccgtCATCCTGCTGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCaccaacc          >  2:16302/5‑100 (MQ=255)
                                                                                        ccgtCATCCTGCTGGATGACGGAATACGGACGACGACGCACCGCTTTGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCaccaacca         >  1:1744820/4‑100 (MQ=255)
                                                                                        ccgtCATCCTGCTGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCaccaacca         >  2:2419216/4‑100 (MQ=255)
                                                                                        aCTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCaccaacca         >  1:1601370/1‑100 (MQ=255)
                                                                                         cgtCATCCTGCTGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCAccaaccaa        >  2:23121/3‑100 (MQ=255)
                                                                                         cgtCATCCTGCTGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGCAGGCGCaccaacca         >  1:1168894/3‑99 (MQ=255)
                                                                                          gtCATCCTGCTGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCaaa       >  1:2740484/2‑100 (MQ=255)
                                                                                           tCATCCTGCTGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCaaa       >  1:1282209/1‑99 (MQ=255)
                                                                                            cATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACg     >  2:2605098/1‑100 (MQ=255)
                                                                                             aTCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACga    >  1:2195902/1‑100 (MQ=255)
                                                                                              tCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACgag   <  1:1986731/100‑1 (MQ=255)
                                                                                              tCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACgag   <  1:2094632/100‑1 (MQ=255)
                                                                                               ccAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACgaga  >  2:1947606/1‑100 (MQ=255)
                                                                                               ccAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACgag   >  1:749366/1‑99 (MQ=255)
                                                                                                 |                                                                                                 
GTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGCGCTTTTTCCACTTCATCCAGCAGGATGACGGAATACGGACGACGACGCACCGCTTCGGTCAGGTAGCCACCTTCTTCATAACCGACATATCCCGGAGGCGCACCAACCAAACGAGA  >  NC_000913/2732050‑2732244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: