Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,810,872 C→T 21.2% H35Y (CAC→TAC) ‡ mprA → transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,8720CT21.2% 219.3 / 44.5 107H35Y (CAC→TAC) mprAtranscriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base C (37/47);  new base T (9/14);  total (46/61)
Fisher's exact test for biased strand distribution p-value = 8.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 122 total aligned reads displayed.

GATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGG  >  NC_000913/2810773‑2810971
                                                                                                   |                                                                                                   
gATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatgc                                                                                                     >  2:2234662/1‑100 (MQ=255)
   aGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcaca                                                                                                  >  2:1561189/1‑100 (MQ=255)
    gTTCGTTTACGCCCATTTAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGTAGTTCCTTCTGCCTCGTCTTTGcattcaca                                                                                                  >  1:890575/1‑99 (MQ=255)
     ttCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatg                                                                                                <  1:219521/100‑1 (MQ=255)
     ttCGTTTACCCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatg                                                                                                <  2:2820980/100‑1 (MQ=255)
        gTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                                                                                             >  2:958099/1‑100 (MQ=255)
        gTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                                                                                             >  2:78311/1‑100 (MQ=255)
          ttACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaag                                                                                           <  1:2208300/100‑1 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                          <  1:1548343/100‑2 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                          <  2:1244603/100‑2 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaag                                                                                           <  1:2757376/99‑1 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagc                                                                                          <  2:2377816/100‑1 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagc                                                                                          <  1:335926/100‑1 (MQ=255)
            aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                         <  1:950156/100‑3 (MQ=255)
             cGCCCATTGAACAGATGCTAAAATTACGCGCCAGCCGCCACGAAGATTTCCCTTATCAGGAGAACCGTCTGACTCGTCTTTGCATCTGCATGCAaaga                                                                                          <  1:2112523/98‑2 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCTGCCGCCTCGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacg                                                                                        <  1:2047309/100‑4 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacg                                                                                        <  1:2342625/100‑4 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                         <  1:172203/99‑3 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaa                                                                                        >  1:1989860/1‑100 (MQ=255)
              gCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaag                                                                                       <  2:1191539/100‑1 (MQ=255)
               cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacgag                                                                                      <  1:2833317/100‑6 (MQ=255)
               cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacgag                                                                                      <  2:2125882/100‑6 (MQ=255)
               cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACCTGCAaagcaagc                                                                                      >  2:268370/1‑100 (MQ=255)
               cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaagc                                                                                      <  2:710817/100‑1 (MQ=255)
                 cATTGAACAAATGCTAAAATTTCGCGCCAGCCGCTACGAAGATTTTCCTTATCAGGAGATCCTTCTGTCTCGTCTTTGCATGTGCATGCAaagacgagtc                                                                                    <  2:1058936/100‑8 (MQ=38)
                 cATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacgagtc                                                                                    <  2:8047/100‑8 (MQ=38)
                 aaTTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTAGTCTTTGCATGCACATGCAAAGCAAgctg                                                                                    >  1:716196/2‑100 (MQ=255)
                     gAACAAATGCTAAAATTTCTCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                <  1:1233147/100‑1 (MQ=255)
                      aaCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                >  1:2399110/1‑99 (MQ=255)
                      aaCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga                                                                               >  1:2284214/1‑100 (MQ=255)
                      aaCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga                                                                               <  2:1242566/100‑1 (MQ=255)
                      aaCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga                                                                               <  2:1244605/100‑1 (MQ=255)
                       aCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgag                                                                              >  1:2480341/1‑100 (MQ=255)
                        cAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgaga                                                                             <  1:417949/100‑1 (MQ=255)
                          aaTGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGaan                                                                           <  1:1505783/100‑2 (MQ=255)
                          aaTGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAAc                                                                           <  1:92835/100‑1 (MQ=255)
                           aTGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATACAAAGCAAGCTGCTGGAGAAcc                                                                          <  1:2816695/100‑1 (MQ=255)
                            tGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCg                                                                         <  2:2527613/100‑1 (MQ=255)
                             gCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGc                                                                        >  1:1859151/1‑100 (MQ=255)
                             gCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGc                                                                        >  1:1459092/1‑100 (MQ=255)
                              cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCa                                                                       >  2:958087/1‑100 (MQ=255)
                              cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAAGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCa                                                                       >  1:1589203/1‑100 (MQ=255)
                               tAAAATTTCGCGCCAGCCGCCACGACGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCaa                                                                      >  2:2846734/1‑100 (MQ=255)
                               tAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCaa                                                                      >  2:2721770/1‑100 (MQ=255)
                                aaaaTTTCGCGCCAGCCGCCACGAATATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAAt                                                                     <  1:363491/100‑1 (MQ=255)
                                aaaaTTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAAt                                                                     >  1:1256479/1‑100 (MQ=255)
                                aaaaTTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAAt                                                                     <  2:869577/100‑1 (MQ=255)
                                    tttCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATaaaa                                                                 <  2:270396/100‑1 (MQ=255)
                                    tttCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATaaaa                                                                 >  2:1009061/1‑100 (MQ=255)
                                       cgcgCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGc                                                              <  2:922998/100‑1 (MQ=255)
                                       cgcgCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGc                                                              >  1:2665906/1‑100 (MQ=255)
                                        gcgcCAGCCGCCACGAAGATTTTCCTTATCAGGAGATACTTCTGACTCGTCTTTGCATGTACATGCAAAGCAAGCTGCTGGCGAACCGCAATAAAATGc                                                              >  1:1316017/1‑99 (MQ=255)
                                         cgcCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTg                                                            >  1:2783865/1‑100 (MQ=255)
                                           ccAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGaa                                                          >  2:1755153/1‑100 (MQ=255)
                                            cAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAg                                                         <  2:2155855/100‑1 (MQ=255)
                                              gccgccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGc                                                       >  1:1906699/1‑100 (MQ=255)
                                                cgccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTc                                                     <  1:2832207/100‑1 (MQ=255)
                                                cgccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTc                                                     <  1:2832300/100‑1 (MQ=255)
                                                 gccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCa                                                    >  2:2456082/1‑100 (MQ=255)
                                                   cACGAAGATTTTCCTTATCAGGGGATCCTGCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAg                                                   <  2:2639015/99‑1 (MQ=255)
                                                   cACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACAGCAATAAAATGCTGAAGGCTCAgg                                                  >  1:604816/1‑100 (MQ=255)
                                                    aCGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAggg                                                 <  1:2674553/100‑1 (MQ=255)
                                                     cGATGATTTTCCTTATCAGGATGTCCTTGTGTCTTGCCGTTGCATCCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                <  1:647268/100‑1 (MQ=255)
                                                     cGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                >  2:2376150/1‑100 (MQ=255)
                                                           ttttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTACATGCAAAGCAAGCTGCTGGAGACCCGCAATAAAATGCTGAAGGCTCAGGGGATTaa                                           >  1:1929677/1‑99 (MQ=255)
                                                              tCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgag                                       >  2:1979409/1‑100 (MQ=255)
                                                                cTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGAc                                     <  2:2116715/100‑1 (MQ=255)
                                                                 ttATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACg                                    <  2:1694564/100‑1 (MQ=255)
                                                                   aTCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgtt                                  >  2:1614470/1‑100 (MQ=255)
                                                                   aTCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgtt                                  >  2:919960/1‑100 (MQ=255)
                                                                    tCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg                                 <  1:261820/100‑1 (MQ=255)
                                                                    tCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg                                 <  1:732614/100‑1 (MQ=255)
                                                                       ggAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt                              >  1:419025/1‑100 (MQ=255)
                                                                         agaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGGAATAAAATGCTGAAGGCTCAGGGGATTAACGCGACGTTGTTTAt                            >  2:455863/1‑100 (MQ=255)
                                                                         agaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGATGAAAAAAAAAAAAAAACTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt                            <  1:1561350/100‑1 (MQ=255)
                                                                          gaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATg                           >  1:1741469/1‑100 (MQ=255)
                                                                           aTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                          <  2:2153515/100‑1 (MQ=255)
                                                                           aTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                          <  2:2153647/100‑1 (MQ=255)
                                                                           aTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                          <  1:2601696/100‑1 (MQ=255)
                                                                               ttCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt                      <  2:2665922/100‑1 (MQ=255)
                                                                               ttCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt                      <  2:2808277/100‑1 (MQ=255)
                                                                                tCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg                     <  1:1965035/100‑1 (MQ=255)
                                                                                tCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg                     <  1:2411717/100‑1 (MQ=255)
                                                                                 agcagcttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa                    >  2:1936146/10‑100 (MQ=38)
                                                                                   gACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAtt                  >  2:428442/1‑100 (MQ=255)
                                                                                   gACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAtt                  >  2:1188942/1‑100 (MQ=255)
                                                                                      tCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg               <  1:2812943/100‑1 (MQ=255)
                                                                                      cttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGACTCAGGGGATTAACGAGACGTTGTTTATGACGTTGATTACg               >  1:1735396/5‑100 (MQ=255)
                                                                                       cGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg               >  1:107885/1‑99 (MQ=255)
                                                                                       cGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGc              <  2:74654/100‑1 (MQ=255)
                                                                                       cGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGc              <  1:1063716/100‑1 (MQ=255)
                                                                                         gcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg            >  1:380885/2‑100 (MQ=255)
                                                                                         gcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg            >  2:2590396/2‑100 (MQ=255)
                                                                                         gcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAAGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGGtt                  >  1:2488405/2‑94 (MQ=255)
                                                                                             tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGt        >  2:2636034/1‑100 (MQ=255)
                                                                                               catgcacacgccAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTCTGGCGTTGATTACGCTGGAGtc       <  1:1694408/99‑1 (MQ=255)
                                                                                                atgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtctc     <  2:822574/100‑1 (MQ=255)
                                                                                                   cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAATATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg  >  1:2187174/1‑100 (MQ=255)
                                                                                                   cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg  <  2:66621/100‑1 (MQ=255)
                                                                                                   cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg  >  2:1922273/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGG  >  NC_000913/2810773‑2810971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: