Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,810,873 A→G 19.5% H35R (CAC→CGC) ‡ mprA → transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,8730AG19.5% 211.4 / 47.2 107H35R (CAC→CGC) mprAtranscriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base A (39/47);  new base G (7/14);  total (46/61)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 123 total aligned reads displayed.

AGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGA  >  NC_000913/2810776‑2810972
                                                                                                 |                                                                                                   
aGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcaca                                                                                                   >  2:1561189/1‑100 (MQ=255)
 gTTCGTTTACGCCCATTTAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGTAGTTCCTTCTGCCTCGTCTTTGcattcaca                                                                                                   >  1:890575/1‑99 (MQ=255)
  ttCGTTTACCCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatg                                                                                                 <  2:2820980/100‑1 (MQ=255)
   tCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatgc                                                                                                <  2:1138014/100‑1 (MQ=255)
     gTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                                                                                              >  2:958099/1‑100 (MQ=255)
     gTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                                                                                              >  1:220335/1‑100 (MQ=255)
     gTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                                                                                              >  2:78311/1‑100 (MQ=255)
       ttACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaag                                                                                            <  1:2208300/100‑1 (MQ=255)
        tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                           <  1:1548343/100‑2 (MQ=255)
        tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                           <  2:1244603/100‑2 (MQ=255)
        tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaag                                                                                            <  1:2757376/99‑1 (MQ=255)
        tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagc                                                                                           >  1:1639946/1‑100 (MQ=255)
        tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagc                                                                                           <  1:335926/100‑1 (MQ=255)
        tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagc                                                                                           <  2:2377816/100‑1 (MQ=255)
         aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                          <  2:260457/100‑3 (MQ=255)
         aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                          <  1:950156/100‑3 (MQ=255)
          cGCCCATTGAACAGATGCTAAAATTACGCGCCAGCCGCCACGAAGATTTCCCTTATCAGGAGAACCGTCTGACTCGTCTTTGCATCTGCATGCAaaga                                                                                           <  1:2112523/98‑2 (MQ=255)
          cGCCCATTGAACAAATGCTAAAATTTCGCGCCTGCCGCCTCGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacg                                                                                         <  1:2047309/100‑4 (MQ=255)
          cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacg                                                                                         <  1:2342625/100‑4 (MQ=255)
          cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                          <  1:172203/99‑3 (MQ=255)
           gCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaag                                                                                        <  2:1191539/100‑1 (MQ=255)
            cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacgag                                                                                       <  2:2125882/100‑6 (MQ=255)
            cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacgag                                                                                       <  1:2833317/100‑6 (MQ=255)
            cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACCTGCAaagcaagc                                                                                       >  2:268370/1‑100 (MQ=255)
            cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaagc                                                                                       <  1:2023513/100‑1 (MQ=255)
            cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaagc                                                                                       <  2:710817/100‑1 (MQ=255)
              cATTGAACAAATGCTAAAATTTCGCGCCAGCCGCTACGAAGATTTTCCTTATCAGGAGATCCTTCTGTCTCGTCTTTGCATGTGCATGCAaagacgagtc                                                                                     <  2:1058936/100‑8 (MQ=38)
              cATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacgagtc                                                                                     <  2:8047/100‑8 (MQ=38)
              aaTTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTAGTCTTTGCATGCACATGCAAAGCAAgctg                                                                                     >  1:716196/2‑100 (MQ=255)
                  gAACAAATGCTAAAATTTCTCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                 <  1:1233147/100‑1 (MQ=255)
                  gAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                 <  2:1781447/100‑1 (MQ=255)
                   aaCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                 >  1:2399110/1‑99 (MQ=255)
                   aaCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga                                                                                >  1:2284214/1‑100 (MQ=255)
                   aaCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga                                                                                <  2:1242566/100‑1 (MQ=255)
                   aaCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga                                                                                <  2:1244605/100‑1 (MQ=255)
                    aCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgag                                                                               <  2:729491/100‑1 (MQ=255)
                    aCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgag                                                                               >  1:2480341/1‑100 (MQ=255)
                     cAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgaga                                                                              <  1:417949/100‑1 (MQ=255)
                     cAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGAATCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgaga                                                                              >  1:589774/1‑100 (MQ=255)
                       aaTGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGaan                                                                            <  1:1505783/100‑2 (MQ=255)
                        aTGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATACAAAGCAAGCTGCTGGAGAAcc                                                                           <  1:2816695/100‑1 (MQ=255)
                         tGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCg                                                                          <  2:2527613/100‑1 (MQ=255)
                          gCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGc                                                                         >  1:1459092/1‑100 (MQ=255)
                          gCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGc                                                                         >  1:1859151/1‑100 (MQ=255)
                           cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCa                                                                        >  2:958087/1‑100 (MQ=255)
                            tAAAATTTCGCGCCAGCCGCCACGACGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCaa                                                                       >  2:2846734/1‑100 (MQ=255)
                            tAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCaa                                                                       >  2:2721770/1‑100 (MQ=255)
                             aaaaTTTCGCGCCAGCCGCCACGAATATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAAt                                                                      <  1:363491/100‑1 (MQ=255)
                             aaaaTTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAAt                                                                      >  1:1256479/1‑100 (MQ=255)
                             aaaaTTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAAt                                                                      <  2:869577/100‑1 (MQ=255)
                                 tttCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATaaaa                                                                  >  2:1009061/1‑100 (MQ=255)
                                    cgcgCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGc                                                               <  2:922998/100‑1 (MQ=255)
                                    cgcgCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGc                                                               >  1:2665906/1‑100 (MQ=255)
                                     gcgcCAGCCGCCACGAAGATTTTCCTTATCAGGAGATACTTCTGACTCGTCTTTGCATGTACATGCAAAGCAAGCTGCTGGCGAACCGCAATAAAATGc                                                               >  1:1316017/1‑99 (MQ=255)
                                        ccAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGaa                                                           <  1:85941/100‑1 (MQ=255)
                                        ccAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGaa                                                           >  2:1755153/1‑100 (MQ=255)
                                         cAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAg                                                          <  2:2155855/100‑1 (MQ=255)
                                           gccgccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGc                                                        >  1:1906699/1‑100 (MQ=255)
                                             cgccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTc                                                      <  1:2832207/100‑1 (MQ=255)
                                              gccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCa                                                     >  2:2456082/1‑100 (MQ=255)
                                                cACGAAGATTTTCCTTATCAGGGGATCCTGCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAg                                                    <  2:2639015/99‑1 (MQ=255)
                                                 aCGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAggg                                                  <  1:2674553/100‑1 (MQ=255)
                                                  cGATGATTTTCCTTATCAGGATGTCCTTGTGTCTTGCCGTTGCATCCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                 <  1:647268/100‑1 (MQ=255)
                                                   gAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGa                                                <  2:2703227/100‑1 (MQ=255)
                                                        ttttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTACATGCAAAGCAAGCTGCTGGAGACCCGCAATAAAATGCTGAAGGCTCAGGGGATTaa                                            >  1:1929677/1‑99 (MQ=255)
                                                           tCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgag                                        >  2:1979409/1‑100 (MQ=255)
                                                             cTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGAc                                      <  2:2116715/100‑1 (MQ=255)
                                                              ttATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACg                                     <  2:1694564/100‑1 (MQ=255)
                                                                aTCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgtt                                   >  2:919960/1‑100 (MQ=255)
                                                                aTCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgtt                                   >  2:1614470/1‑100 (MQ=255)
                                                                 tCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg                                  <  1:732614/100‑1 (MQ=255)
                                                                    ggAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt                               >  1:419025/1‑100 (MQ=255)
                                                                      agaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGGAATAAAATGCTGAAGGCTCAGGGGATTAACGCGACGTTGTTTAt                             >  2:455863/1‑100 (MQ=255)
                                                                      agaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGATGAAAAAAAAAAAAAAACTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt                             <  1:1561350/100‑1 (MQ=255)
                                                                       gaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATg                            >  1:1741469/1‑100 (MQ=255)
                                                                       gaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATg                            <  1:2474345/100‑1 (MQ=255)
                                                                        aTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                           <  2:2153515/100‑1 (MQ=255)
                                                                        aTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                           <  1:2601696/100‑1 (MQ=255)
                                                                        aTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                           <  2:2153647/100‑1 (MQ=255)
                                                                          ccTGCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                           <  1:2622917/98‑1 (MQ=255)
                                                                            ttCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt                       <  2:2808277/100‑1 (MQ=255)
                                                                            ttCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt                       <  2:2665922/100‑1 (MQ=255)
                                                                             tCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg                      <  1:1965035/100‑1 (MQ=255)
                                                                              agcagcttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa                     >  2:1936146/10‑100 (MQ=38)
                                                                               tGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt                    >  2:2243097/1‑100 (MQ=255)
                                                                                gACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAtt                   >  2:428442/1‑100 (MQ=255)
                                                                                   tCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg                <  1:2812943/100‑1 (MQ=255)
                                                                                   cttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGACTCAGGGGATTAACGAGACGTTGTTTATGACGTTGATTACg                >  1:1735396/5‑100 (MQ=255)
                                                                                    cGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg                >  1:107885/1‑99 (MQ=255)
                                                                                    cGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGc               <  1:1063716/100‑1 (MQ=255)
                                                                                      gcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg             >  1:304795/2‑100 (MQ=255)
                                                                                      gcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg             >  2:2590396/2‑100 (MQ=255)
                                                                                      gcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAAGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGGtt                   >  1:2488405/2‑94 (MQ=255)
                                                                                          tgcatgcaCAAGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa           <  1:2815811/98‑1 (MQ=255)
                                                                                            catgcacacgccAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTCTGGCGTTGATTACGCTGGAGtc        <  1:1694408/99‑1 (MQ=255)
                                                                                             atgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtctc      <  2:822574/100‑1 (MQ=255)
                                                                                                cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAATATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg   >  1:2187174/1‑100 (MQ=255)
                                                                                                cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg   <  2:66621/100‑1 (MQ=255)
                                                                                                 acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGa  >  1:568057/1‑100 (MQ=255)
                                                                                                 acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGa  <  1:282327/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
AGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGA  >  NC_000913/2810776‑2810972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: