Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 236,569 C→T 17.2% A168V (GCC→GTC)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913236,5690CT17.2% 223.0 / 36.1 99A168V (GCC→GTC) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base C (44/37);  new base T (10/7);  total (54/45)
Fisher's exact test for biased strand distribution p-value = 7.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 108 total aligned reads displayed.

GGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAAC  >  NC_000913/236470‑236668
                                                                                                   |                                                                                                   
ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCGCGGGGCATTACTCGATGc                                                                                                     <  1:1754866/100‑1 (MQ=255)
ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGc                                                                                                     >  1:645494/1‑100 (MQ=255)
ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGc                                                                                                     >  2:776497/1‑100 (MQ=255)
ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGc                                                                                                     >  1:1329227/1‑100 (MQ=255)
  aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGccc                                                                                                   >  2:832916/1‑100 (MQ=255)
  aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGccc                                                                                                   <  2:1411783/100‑1 (MQ=255)
  aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGccc                                                                                                   >  2:2091975/1‑100 (MQ=255)
   aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCa                                                                                                  >  2:593190/1‑100 (MQ=255)
   aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCa                                                                                                  <  1:145542/100‑1 (MQ=255)
      tGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGAt                                                                                               <  1:116999/100‑1 (MQ=255)
       gTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATc                                                                                              >  1:26620/1‑100 (MQ=255)
       gTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATc                                                                                              <  2:2352387/100‑1 (MQ=255)
        tttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATcc                                                                                             >  2:987843/1‑100 (MQ=255)
         ttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCt                                                                                            <  1:2751720/100‑1 (MQ=255)
         ttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCt                                                                                            >  2:2396516/1‑100 (MQ=255)
            ccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGc                                                                                         >  2:1033287/1‑100 (MQ=255)
            ccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGc                                                                                         <  2:314847/100‑1 (MQ=255)
             cGGTAAGCGAAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGACCGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCg                                                                                        <  1:1010047/100‑1 (MQ=255)
              ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACATTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCgg                                                                                       <  2:1424766/100‑1 (MQ=255)
              ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCg                                                                                        <  2:1606944/99‑1 (MQ=255)
                 aaGCGCAACAGCCTCGATGCGTTATGTTCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAg                                                                                    <  2:625962/100‑1 (MQ=255)
                 aaGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAg                                                                                    <  2:2188955/100‑1 (MQ=255)
                 aaGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAg                                                                                    <  1:1793841/100‑1 (MQ=255)
                  aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGt                                                                                   >  2:37683/1‑100 (MQ=255)
                  aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt                                                                                   >  2:249657/1‑100 (MQ=255)
                  aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt                                                                                   >  2:546364/1‑100 (MQ=255)
                  aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt                                                                                   >  1:1676231/1‑100 (MQ=255)
                  aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACACTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt                                                                                   >  2:159992/1‑100 (MQ=255)
                    cgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGttt                                                                                 >  2:1416633/1‑100 (MQ=255)
                      cAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTAt                                                                               >  1:1607252/1‑100 (MQ=255)
                       aaCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATc                                                                              >  1:734818/1‑100 (MQ=255)
                        aCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCt                                                                             >  2:2069982/1‑100 (MQ=255)
                         cAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTg                                                                            <  1:832913/100‑1 (MQ=255)
                           gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGc                                                                          >  1:1071643/1‑100 (MQ=255)
                            ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCg                                                                         >  1:2294557/1‑100 (MQ=255)
                              tCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGAt                                                                       >  2:2544286/1‑100 (MQ=255)
                              tCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGAt                                                                       >  1:1218200/1‑100 (MQ=255)
                                   gCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCg                                                                  >  1:1311126/1‑100 (MQ=255)
                                       tATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg                                                              <  1:2075/100‑1 (MQ=255)
                                         tgtgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc                                                            <  2:1860659/100‑1 (MQ=255)
                                           tgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa                                                          >  2:702080/1‑100 (MQ=255)
                                            gCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaaa                                                         >  2:519162/1‑100 (MQ=255)
                                               cGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACg                                                       <  1:246294/99‑1 (MQ=255)
                                               cGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGt                                                      >  1:2823599/1‑100 (MQ=255)
                                               cGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGt                                                      >  2:1153145/1‑100 (MQ=255)
                                                gCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTc                                                     >  2:127253/1‑100 (MQ=255)
                                                 cTACGAAATAGATAACAGTAAACGTACTCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                                                    <  1:1765840/100‑1 (MQ=255)
                                                 cTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                                                    >  2:2589415/1‑100 (MQ=255)
                                                    cGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGAt                                                  <  2:399892/99‑1 (MQ=255)
                                                    cGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg                                                 <  2:734821/100‑1 (MQ=255)
                                                     gAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATgg                                                >  1:2335531/1‑100 (MQ=255)
                                                     gAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATgg                                                >  2:1776296/1‑100 (MQ=255)
                                                      aaaTAGATAACAGTAAACGAACGCTGCACGGTGCATTACGCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTTGTCAAACGTCGATg                                                 <  1:480848/98‑1 (MQ=255)
                                                        aTAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCtt                                             >  1:1480752/1‑100 (MQ=255)
                                                            aTAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGc                                         <  2:1290852/100‑1 (MQ=255)
                                                             tAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg                                        >  2:2206672/1‑100 (MQ=255)
                                                             tAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg                                        <  1:202773/100‑1 (MQ=255)
                                                              aaCAGTAAACGAACGATGCACGGGGCATTACTCGATGACCTGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg                                        <  2:2589118/99‑1 (MQ=255)
                                                               aCAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt                                      <  2:1702459/100‑1 (MQ=255)
                                                                cAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                     >  2:2866434/1‑100 (MQ=255)
                                                                cAGTAAACGAACGCTGCACGGGGCATTACTCGATGCACAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTggg                                        >  1:2908258/1‑95 (MQ=255)
                                                                cAGTAAACGAACGCTGCACGGGGCATTACGCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                     <  1:1235388/100‑1 (MQ=255)
                                                                 aGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg                                          >  1:2168998/1‑94 (MQ=255)
                                                                    aaaCGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAg                                 <  1:2392640/100‑1 (MQ=255)
                                                                    aaaCGAACACTGCACGGGGCATTACTCGATGCCAAGCTCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGGTGGATTTTGCGATGGAAg                                 >  1:1116901/1‑100 (MQ=255)
                                                                     aacgaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAgg                                >  2:2472632/1‑100 (MQ=255)
                                                                      acgaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGga                               >  1:1612019/1‑100 (MQ=255)
                                                                       cgaacgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgag                              <  2:1506209/100‑1 (MQ=255)
                                                                        gaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgaga                             >  1:105554/1‑100 (MQ=255)
                                                                         aacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgagag                            <  1:2720709/100‑1 (MQ=255)
                                                                         aacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgagag                            <  1:192156/100‑1 (MQ=255)
                                                                          acgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgagaga                           >  1:2575080/1‑100 (MQ=255)
                                                                            gCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGaca                         >  1:2829784/1‑100 (MQ=255)
                                                                            gCTGCACGGGGCAGTTCTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGaca                         <  2:2868243/100‑1 (MQ=255)
                                                                            cgatctcGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGaca                         <  2:1364186/94‑1 (MQ=255)
                                                                             cTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGacac                        >  2:1544067/1‑100 (MQ=255)
                                                                              tGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGAcaca                       <  2:772144/100‑1 (MQ=255)
                                                                               gCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaa                      >  1:222467/1‑100 (MQ=255)
                                                                                cACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaac                     >  1:1209684/1‑100 (MQ=255)
                                                                                 aCGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaaca                    >  1:2064858/1‑100 (MQ=255)
                                                                                 aCGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaaca                    >  2:518536/1‑100 (MQ=255)
                                                                                 aCGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaaca                    >  2:2099850/1‑100 (MQ=255)
                                                                                  cGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAg                   >  2:1878468/1‑100 (MQ=255)
                                                                                  cGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGacaagatcg                   >  1:1364181/1‑94 (MQ=255)
                                                                                   ggggCATTACTCGATGCCCAGAGCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGAcaca                       <  2:315392/95‑1 (MQ=255)
                                                                                      gCATTACTCGATGCCCAGATCCTTGCGGAAGTGTAGCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaac               <  1:517783/100‑1 (MQ=255)
                                                                                       cATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaaca              >  2:1723712/1‑100 (MQ=255)
                                                                                          tACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAg            <  1:2807398/99‑1 (MQ=255)
                                                                                            cTCGATGCCCAGCTCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTg         >  2:1914052/1‑100 (MQ=255)
                                                                                            cTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTg         <  2:1152240/100‑1 (MQ=255)
                                                                                             tCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGaa       >  2:2541578/1‑100 (MQ=255)
                                                                                              cGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGaa       <  2:1026784/100‑1 (MQ=255)
                                                                                              cGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGaa       >  2:1348785/1‑100 (MQ=255)
                                                                                              cGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGaa       <  1:66612/100‑1 (MQ=255)
                                                                                               gATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAg      >  1:641354/1‑100 (MQ=255)
                                                                                               gATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAg      >  2:2411456/1‑100 (MQ=255)
                                                                                               gATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAg      <  2:117510/100‑1 (MQ=255)
                                                                                                aTGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGc     <  2:494264/100‑1 (MQ=255)
                                                                                                 tGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGca    <  2:780918/100‑1 (MQ=255)
                                                                                                   cccAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGcaac  >  1:1847219/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAAC  >  NC_000913/236470‑236668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: