Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,838,222 A→T 17.8% intergenic (‑117/+32) hydN ← / ← ascG formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,838,2220AT17.8% 319.2 / 57.8 134intergenic (‑117/+32)hydN/ascGformate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base A (59/51);  new base T (12/12);  total (71/63)
Fisher's exact test for biased strand distribution p-value = 8.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 137 total aligned reads displayed.

CAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAG  >  NC_000913/2838124‑2838320
                                                                                                  |                                                                                                  
cAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGac                                                                                                   <  2:2745322/100‑1 (MQ=255)
       tGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGt                                                                                             >  1:2084829/1‑99 (MQ=255)
         cgcCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGAc                                                                                          >  2:764701/1‑100 (MQ=255)
            cGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGAt                                                                                       >  2:744941/1‑100 (MQ=255)
             gATACATTCACAAATCATGCTAGTTTGTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATg                                                                                      <  1:865399/100‑1 (MQ=255)
             gATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATg                                                                                      <  2:982376/100‑1 (MQ=255)
             gATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATg                                                                                      >  1:1383779/1‑100 (MQ=255)
             gATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATg                                                                                      >  2:1866103/1‑100 (MQ=255)
                aCATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACa                                                                                   <  2:1453510/100‑1 (MQ=255)
                 cATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGAcgt                                                                                  <  2:1970075/100‑3 (MQ=255)
                 cATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAt                                                                                  >  2:2466549/1‑100 (MQ=255)
                  aTTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAtg                                                                                 >  2:1374318/1‑100 (MQ=255)
                  aTTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAtg                                                                                 <  2:1355006/100‑1 (MQ=255)
                  aTTCACAAATCATGCCAGTTTTAAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGACAtc                                                                                 <  2:2733860/100‑2 (MQ=38)
                   ttCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGACAtcg                                                                                <  2:2078159/100‑3 (MQ=255)
                   ttCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGACAtcg                                                                                <  2:698767/100‑3 (MQ=255)
                   ttCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAtgt                                                                                >  1:2597384/1‑100 (MQ=255)
                   ttCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGCAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAtgt                                                                                <  2:1677470/100‑1 (MQ=255)
                   ttCACAAATCATGCCAGTTTTTAATGTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGACAtcg                                                                                <  1:1392191/100‑3 (MQ=38)
                    tCACAAATCATGCCAGTTTTTTATTTACTGATATTTCAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGACAtcg                                                                                <  1:1963169/99‑3 (MQ=37)
                    tCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAtgtg                                                                               <  1:1517145/100‑1 (MQ=255)
                     cacaAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCGTCGTCACTAGTGTCGATGACAtcgtt                                                                              <  2:1779643/100‑5 (MQ=38)
                     cacaAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtga                                                                              <  2:325263/100‑1 (MQ=255)
                     cacaAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGCGACGATGACATGtga                                                                              >  1:2272126/1‑100 (MQ=255)
                         aaTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGAc                                                                          <  2:2351856/100‑1 (MQ=255)
                            cATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAGTTTCTGTAATGCAGGAAAAACGATGTCATCGACAGTAGTGACGATGACATGTGATGACa                                                                         >  1:1801483/1‑98 (MQ=255)
                            cATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAAt                                                                       >  2:44670/1‑100 (MQ=255)
                               gCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGtt                                                                    >  2:343605/1‑100 (MQ=255)
                                ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGttt                                                                   >  2:626402/1‑100 (MQ=255)
                                ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGttt                                                                   >  1:2614142/1‑100 (MQ=255)
                                 cAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGTAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTa                                                                  <  1:2780416/100‑1 (MQ=255)
                                 cAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTa                                                                  <  2:2541154/100‑1 (MQ=255)
                                     ttttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATcgc                                                              <  2:2500127/100‑1 (MQ=255)
                                      tttAATTTACTGTTATTTAAGGAAATTATTTTCTGTAATAAAAAAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATcgcg                                                             <  1:631758/100‑1 (MQ=255)
                                        tAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGaa                                                           <  2:2638638/100‑1 (MQ=255)
                                         aaTTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAg                                                          <  1:1632602/100‑1 (MQ=255)
                                          aTTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAgg                                                         <  1:151173/100‑1 (MQ=255)
                                          aTTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAgg                                                         <  2:1887488/100‑1 (MQ=255)
                                          aTTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAgg                                                         <  2:2762573/100‑1 (MQ=255)
                                           tttACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATTATATGTGTTGACAATGTTTATCTCGATgg                                                         <  1:2135590/99‑1 (MQ=255)
                                               cTGTTATTTAAGGCAATTAATATCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGCGCaa                                                    >  1:74683/1‑100 (MQ=255)
                                               cTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGCCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCaa                                                    >  1:2279036/1‑100 (MQ=255)
                                               cTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCaa                                                    >  1:291153/1‑100 (MQ=255)
                                               cTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCaa                                                    >  1:1837198/1‑100 (MQ=255)
                                               cTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCaa                                                    >  2:1442893/1‑100 (MQ=255)
                                                tGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAt                                                   <  2:974742/100‑1 (MQ=255)
                                                 gTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtg                                                  >  1:683755/1‑100 (MQ=255)
                                                 gTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtg                                                  <  2:2404146/100‑1 (MQ=255)
                                                 gTTATTGAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATTTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtg                                                  <  1:573500/100‑1 (MQ=255)
                                                   tATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgag                                                >  2:353194/1‑100 (MQ=255)
                                                    aTTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagt                                               <  2:1522820/100‑1 (MQ=255)
                                                    aTTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagt                                               >  1:770840/1‑100 (MQ=255)
                                                     tttAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagtg                                              >  1:81437/1‑100 (MQ=255)
                                                          ggAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGATACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTcg                                         >  1:2713611/1‑100 (MQ=255)
                                                          ggAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTcg                                         <  2:2002810/100‑1 (MQ=255)
                                                             aattaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGATTATCGCGAAGGAGCAATgagtgagt                                           >  1:1925531/1‑95 (MQ=255)
                                                              attaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCgcggc                                     <  2:192088/100‑1 (MQ=255)
                                                              attaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCgcggc                                     <  1:894236/100‑1 (MQ=255)
                                                                 aattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGa                                  <  2:2612109/100‑1 (MQ=255)
                                                                  attTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGAt                                 >  2:503965/1‑100 (MQ=255)
                                                                  attTCTGTAATGCAGGAAAAACGATGTCATCGACACGAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGAt                                 <  2:1838418/100‑1 (MQ=255)
                                                                  attGCTGTAATGCAGGAAAAACGATGTCATCGACACTAGGGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGAt                                 <  1:253079/100‑1 (MQ=255)
                                                                   tttCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATc                                >  2:2714529/1‑100 (MQ=255)
                                                                    ttCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCa                               <  1:2444632/100‑1 (MQ=255)
                                                                     tCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAg                              <  1:918321/100‑1 (MQ=255)
                                                                     tCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAg                              <  1:1397050/100‑1 (MQ=255)
                                                                           aTGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTcc                        >  2:2295844/1‑100 (MQ=255)
                                                                            ttcAGGAAAAACTATGTCATCGACACTAGTGACGATGGCATGTGATAACGATGTTTATCGCGAAGGAACAATGAGTGAGTCGCGGCGGCTCAGTTTTcc                        >  1:1615614/3‑99 (MQ=255)
                                                                              ctggAAAAACGATATCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCt                     >  2:1977247/3‑100 (MQ=255)
                                                                              cAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCt                     >  1:1012651/1‑100 (MQ=255)
                                                                               aGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTg                    <  1:1632249/100‑1 (MQ=255)
                                                                                ggAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGa                   >  2:393369/1‑100 (MQ=255)
                                                                                ggAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGa                   >  1:747240/1‑100 (MQ=255)
                                                                                  ttccgatctGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAg                 <  1:2818419/92‑1 (MQ=38)
                                                                                  tcatcacaTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAg                 >  1:2442855/8‑100 (MQ=38)
                                                                                    atcacaTGTCATCGTCACTGGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGGGTGAGTCGCGGGGGATGGGTGTTCGGATGAAgg                >  1:2521880/6‑99 (MQ=21)
                                                                                    aaaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGt               <  2:2120405/100‑1 (MQ=255)
                                                                                       acaTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAACGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGtttt            >  2:1241292/3‑100 (MQ=38)
                                                                                        caTGTCATCGTCACTAGTGTCGATGACATGTGATGACATTGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTc           >  1:1075783/2‑100 (MQ=38)
                                                                                        caTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTc           >  2:155788/2‑100 (MQ=255)
                                                                                        caTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTc           >  1:1954374/2‑100 (MQ=255)
                                                                                        caTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTc           >  1:460039/2‑100 (MQ=255)
                                                                                        caTGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCAGCTGAAGGTTTTc           >  1:862894/2‑100 (MQ=255)
                                                                                         aTGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcg          <  1:1220114/100‑1 (MQ=255)
                                                                                         aTGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcg          >  2:1103428/1‑100 (MQ=255)
                                                                                         aTGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTGTTCCGCTGAAGGTTTTcg          <  1:945136/100‑1 (MQ=255)
                                                                                          tGTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGttt             >  1:1105312/1‑96 (MQ=255)
                                                                                          tGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGATGGTTTTcgg         <  2:51613/100‑1 (MQ=255)
                                                                                           ntCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTGTTCCGCTGAAGGTTTTcggc        >  1:721994/2‑100 (MQ=255)
                                                                                           gTCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAgagatcggaa        >  2:2818437/1‑91 (MQ=38)
                                                                                            tCATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGATCAATGAGGGAGTCGCGGCGGATCAGTTTTCCGCTGAAGATTTTcggcg       >  1:602600/1‑100 (MQ=255)
                                                                                             cATCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAACGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggcgg      <  1:1241287/100‑1 (MQ=255)
                                                                                             cATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggcgg      >  2:1310538/1‑100 (MQ=255)
                                                                                             cATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggcgg      <  1:249271/100‑1 (MQ=255)
                                                                                             cATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggcgg      >  1:2344913/1‑100 (MQ=255)
                                                                                             cATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggcgg      >  2:1401850/1‑100 (MQ=255)
                                                                                             cATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggcgg      >  1:1163903/1‑100 (MQ=255)
                                                                                              aTCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGt     >  1:2069973/1‑100 (MQ=255)
                                                                                              aTCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGt     >  2:1317726/1‑100 (MQ=255)
                                                                                                 gACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTgag  <  2:2350785/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
CAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAG  >  NC_000913/2838124‑2838320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑