Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,900,327 A→C 15.0% intergenic (‑54/‑265) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,900,3270AC15.0% 294.4 / 45.2 120intergenic (‑54/‑265)ygcW/yqcEputative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (56/44);  new base C (15/3);  total (71/49)
Fisher's exact test for biased strand distribution p-value = 3.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

Only 100 of 125 total aligned reads displayed.

GGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGT  >  NC_000913/2900229‑2900426
                                                                                                  |                                                                                                   
ggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCgag                                                                                                    <  2:2388515/100‑1 (MQ=255)
  agaAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGagag                                                                                                  >  2:1673913/1‑100 (MQ=255)
  agaAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                                                                 >  2:2345096/1‑100 (MQ=255)
         aTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGtt                                                                                           <  2:2034089/100‑1 (MQ=255)
            cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAcc                                                                                        >  2:1065258/1‑100 (MQ=255)
            cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGAAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTAcc                                                                                        <  2:1710587/100‑1 (MQ=37)
             aatGAGAACACATGGAGAGATTAGAATGACATAGTTAGCTCCAATTTCCGTAAAAAAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTAcc                                                                                        <  1:183994/97‑1 (MQ=16)
                gagaACGCATTGAGAGATTCCATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtc                                                                                    >  1:1777674/1‑100 (MQ=255)
                 agaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtct                                                                                   <  1:1740736/100‑1 (MQ=255)
                 agaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtct                                                                                   >  2:828797/1‑100 (MQ=255)
                  gaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                  <  2:1144932/100‑1 (MQ=255)
                  gaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                  <  2:1031120/100‑1 (MQ=255)
                  gaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                  >  1:1680954/1‑100 (MQ=255)
                   aaCGCATTGAGAGATTCGATTGGCATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                  <  1:1808594/99‑1 (MQ=255)
                   aaCGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCa                                                                                 >  1:1591044/1‑100 (MQ=255)
                    acttaTTGATAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCAt                                                                                <  2:2494118/96‑1 (MQ=255)
                    aCGTATTGAGAGATGCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                  <  1:1808656/98‑1 (MQ=255)
                       cATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtcttttttt                                                                             >  2:2811440/1‑94 (MQ=255)
                        aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtc                                                                            >  1:1663643/1‑100 (MQ=255)
                        aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtc                                                                            <  2:548431/100‑1 (MQ=255)
                           gagagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATctct                                                                         >  1:2750224/1‑100 (MQ=255)
                           gagagaTTCGATTGACATAGTTAGCCCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATctct                                                                         >  2:2039753/1‑100 (MQ=255)
                           gagagaTTCGATTGAAATAGTTAGCTGAAATATCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCACCTAGAGAGGGAAAATAACTTTCTCATTAtct                                                                           >  1:176345/1‑98 (MQ=255)
                            agagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTg                                                                        >  1:2888031/1‑100 (MQ=255)
                               gaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                       >  2:2771666/1‑98 (MQ=255)
                               gaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTa                                                                     >  1:2144048/1‑100 (MQ=255)
                                 tttcaTTGACATAGTCAGCTCTAATAACCGAAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGGGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                       <  1:2490353/92‑1 (MQ=37)
                                    gATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAACTTTCTCATTATCTCTGGTAACTgg                                                                >  1:1382850/1‑100 (MQ=255)
                                     aTTGACATAGTTAGCTCCAATTTCCGTAAAGAAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGga                                                               <  2:2200139/100‑1 (MQ=255)
                                        naCATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGAt                                                            >  1:554482/2‑100 (MQ=255)
                                        gACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGAt                                                            >  1:2271978/1‑100 (MQ=255)
                                        gACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGAt                                                            >  2:1960145/1‑100 (MQ=255)
                                        gACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGAt                                                            >  2:2460272/1‑100 (MQ=255)
                                        gACATAGTTAGCACCAACTACCGTAAGGCAAAAAAAAGGAAACGGAACTTCGCCCGCTGGAGGGAAGTTACCTTTCTCATTATCTCTGGTTATTGgag                                                              <  1:1958867/98‑1 (MQ=255)
                                         aCATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATa                                                           >  2:1628110/1‑100 (MQ=255)
                                             aGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATAAtga                                                       <  2:2467177/100‑3 (MQ=38)
                                              gTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAtttt                                                      >  2:885114/1‑100 (MQ=255)
                                              gTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAtttt                                                      >  1:1264228/1‑100 (MQ=255)
                                                tAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTa                                                    <  2:888434/100‑1 (MQ=255)
                                                tAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTa                                                    <  2:888373/100‑1 (MQ=255)
                                                 aGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                   <  1:2674791/100‑1 (MQ=255)
                                                 aGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                   <  1:272191/100‑1 (MQ=255)
                                                 aGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                   <  2:376627/100‑1 (MQ=255)
                                                  gCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGc                                                  <  1:2771648/100‑1 (MQ=255)
                                                  gCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGc                                                  >  1:255110/1‑100 (MQ=255)
                                                  gCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGc                                                  <  2:837642/100‑1 (MQ=255)
                                                  gCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGc                                                  >  1:413729/1‑100 (MQ=255)
                                                  gCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGc                                                  >  1:1414551/1‑100 (MQ=255)
                                                   cTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCa                                                 >  2:1430000/1‑100 (MQ=255)
                                                     ccAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATa                                               <  2:2707238/100‑1 (MQ=255)
                                                     ccAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAAATGGAGATAATTTTTAGCATa                                               >  1:380205/1‑100 (MQ=255)
                                                             ngTAAGGCAAAAAATACGAAAGGCAACTTCCGGCGAGAGAGCGAAGTTACCTTNCTCATTATCTCTGGTAAATGGAGATAATTTTTAGACTAAGGGCATa                                       >  1:1304195/2‑100 (MQ=255)
                                                              gTAAGGAAAAAAAAAAGAAAGGTAACTTCCCCCGAGAGAGGGAAGTTACCTTGCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATa                                       <  1:707238/99‑1 (MQ=255)
                                                                aaGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGAGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACt                                    <  1:2742945/100‑1 (MQ=255)
                                                                  ggCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGc                                  >  2:410456/1‑100 (MQ=255)
                                                                  ggCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGc                                  <  2:2114/100‑1 (MQ=255)
                                                                  ggCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGc                                  >  1:1198268/1‑100 (MQ=255)
                                                                  gcaaaaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGc                                  >  1:1963620/4‑100 (MQ=255)
                                                                    cAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTa                                <  2:678168/100‑1 (MQ=255)
                                                                    cAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGCGCATAACTGCTa                                >  1:475427/1‑100 (MQ=255)
                                                                     aaaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTaa                               <  2:886839/100‑1 (MQ=255)
                                                                      aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGCCATAACTGCTAAt                              >  1:1094495/1‑100 (MQ=255)
                                                                       aaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtg                             <  1:1662474/100‑1 (MQ=255)
                                                                       aaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtg                             <  2:1360665/100‑1 (MQ=255)
                                                                       aaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAACTTTCTCATTATCTCTGGTAACTGGAGATAAGTTTTAGCATAAGGGCATAACTGCTAAtg                             <  1:1262260/100‑1 (MQ=255)
                                                                         aaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtg                           >  2:1689558/1‑100 (MQ=255)
                                                                          aaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGa                          >  2:1469857/1‑100 (MQ=255)
                                                                          aaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGa                          >  2:362643/1‑100 (MQ=255)
                                                                           aaagaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGAt                         <  2:2573905/100‑1 (MQ=255)
                                                                              gaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGt                      <  2:2383291/100‑1 (MQ=255)
                                                                               aaagGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTa                     >  2:2364208/1‑100 (MQ=37)
                                                                               aaagGTAACTTCCCCCGCCAGAGGGAAGTTACCTTTCTCATTAACTCTGATCACTGGAGATAATTTTTAGAATAAGGGCATAACTGCTAATGTCCTCGTa                     >  1:950587/1‑100 (MQ=255)
                                                                                aagGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTaa                    >  1:1114589/1‑100 (MQ=37)
                                                                                 agGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAAt                   >  2:824508/1‑100 (MQ=37)
                                                                                 agGTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCCCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATTGTAAt                   >  1:2509277/1‑100 (MQ=37)
                                                                                 agGTAACTTCCCTCTCGCGGGGGAAGGTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAAATGCTAATGTGATCGTAAt                   >  1:2375875/1‑100 (MQ=37)
                                                                                  ggTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAAt                   >  1:2489098/1‑99 (MQ=37)
                                                                                  ggTAACTTCCCTCTCGCGGGGGAAGTTACCTTACTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATc                  >  2:2765743/1‑100 (MQ=37)
                                                                                  ggTAACTTCCCTATCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATc                  >  2:1918001/1‑100 (MQ=37)
                                                                                  ggTAACTTCCCCCGCGAGAGGGAAGTTACCTTTTTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGTGCATAACTGCTAATGTGATCGTAATc                  >  1:100949/1‑100 (MQ=255)
                                                                                   gTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATca                 >  1:610172/1‑100 (MQ=37)
                                                                                   gTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATca                 >  2:2227426/1‑100 (MQ=255)
                                                                                    tAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcac                >  2:659645/1‑100 (MQ=255)
                                                                                    tAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcac                >  2:234891/1‑100 (MQ=255)
                                                                                     aaCTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcaca               >  2:331621/1‑100 (MQ=38)
                                                                                     aaCTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcaca               >  1:1114648/1‑100 (MQ=38)
                                                                                      aCTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAg              <  1:362642/100‑1 (MQ=255)
                                                                                       cTTCCCCTGCGCGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATGAGGGCATAACTGCTAATGTGATCGTAATCACAgt             >  1:1529478/1‑100 (MQ=255)
                                                                                       cTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgt             <  1:885111/100‑1 (MQ=255)
                                                                                        ttCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtg            <  1:255583/100‑1 (MQ=255)
                                                                                         tCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtgt           >  2:119963/1‑100 (MQ=255)
                                                                                         tCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtg            <  1:2039191/99‑1 (MQ=255)
                                                                                           ccccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGa         >  2:1062089/1‑100 (MQ=255)
                                                                                            cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat        >  1:2180585/1‑100 (MQ=255)
                                                                                            cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat        >  1:470194/1‑100 (MQ=255)
                                                                                             ccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATATGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGata       >  1:1224213/1‑100 (MQ=255)
                                                                                                cgAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTc    >  2:1808260/1‑100 (MQ=255)
                                                                                                 gagaGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGTGCATAACTGCTAATGTGATCGTAATCACAGTGTGat        <  1:1765531/95‑1 (MQ=255)
                                                                                                 gagaGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCg   <  2:2359603/100‑1 (MQ=255)
                                                                                                  agagGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGt  <  1:738130/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGT  >  NC_000913/2900229‑2900426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: