Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,933,398 A→T 15.4% H97Q (CAT→CAA fucA ← L‑fuculose‑1‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,933,3980AT15.4% 293.0 / 41.3 117H97Q (CAT→CAAfucAL‑fuculose‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (42/56);  new base T (2/16);  total (45/72)
Fisher's exact test for biased strand distribution p-value = 1.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

Only 100 of 122 total aligned reads displayed.

GGTCGCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTCA  >  NC_000913/2933299‑2933497
                                                                                                   |                                                                                                   
ggTCGCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCaa                                                                                                     >  1:1505069/1‑100 (MQ=255)
 gTCGCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAAt                                                                                                    <  1:2913853/100‑1 (MQ=255)
 gTCGCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAAt                                                                                                    >  1:1448179/1‑100 (MQ=255)
  tCGCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCTCAATGATGTAGTGAATAGTGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATg                                                                                                   <  1:1304718/100‑1 (MQ=255)
   cGCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGa                                                                                                  >  2:1535823/1‑100 (MQ=255)
    gCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGaa                                                                                                 >  2:1215328/1‑100 (MQ=255)
      aTAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACg                                                                                               <  2:1628990/100‑1 (MQ=255)
        aaGGCGCGCAAGGAATAGAATTACCTCCAGCCGCCGTAATCATGTAGTTAATAGCGGGGATCGATCGGTTAAGAATGGAATCTGCCGTTCATTGCACGGc                                                                                             <  1:587561/100‑1 (MQ=255)
        aaGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGc                                                                                             <  2:2105689/100‑1 (MQ=255)
         aGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAACCTGCCGTGCAATGAAc                                                                                                <  1:1440495/96‑1 (MQ=255)
         aGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGc                                                                                             <  1:587259/99‑1 (MQ=255)
         aGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCa                                                                                            >  2:2838298/1‑100 (MQ=255)
          ggggggcgaGGAATAGAGTTACCGCCGGCCGGCGCGATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACGGCCGTTCATTGCACGGCa                                                                                            <  1:1837251/91‑1 (MQ=255)
          ggCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGCag                                                                                           <  1:146398/100‑2 (MQ=255)
          ggCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGCag                                                                                           <  2:1790030/100‑2 (MQ=255)
           gctcgcAAGGAATAGAATTACCGTCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGCagt                                                                                          <  1:933045/97‑3 (MQ=255)
           gcgcgcAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGCag                                                                                           <  1:1337929/99‑2 (MQ=255)
            cgcgcAAGGAATAGAATTACTGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATAGATCGGTTAAGAATGGACACTGCGGTGCCATGAACGACATg                                                                                          >  1:1210142/1‑99 (MQ=255)
            cgcgcAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGCagtt                                                                                         <  2:2516173/100‑4 (MQ=255)
            cgcgcAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGa                                                                                         >  2:58202/1‑100 (MQ=255)
            cgcgcAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGa                                                                                         >  2:2195572/1‑100 (MQ=255)
            cgcgcAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGa                                                                                         >  1:2083049/1‑100 (MQ=255)
               gcAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGCagtttcc                                                                                      <  2:2474074/100‑7 (MQ=255)
               gcAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGCagtttc                                                                                       <  1:2329836/99‑6 (MQ=255)
               gcAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATtg                                                                                      >  2:1782970/1‑100 (MQ=255)
               gcAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATtg                                                                                      <  1:1193478/100‑1 (MQ=255)
               gcAAGGAATAGAACTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGCagtttcc                                                                                      <  2:2740532/100‑7 (MQ=255)
                 aaGGAATAGAACTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATtgtg                                                                                    <  1:2813545/100‑1 (MQ=255)
                   ggAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTTCATTGCACGGCagtttccattc                                                                                  <  1:1515290/100‑11 (MQ=37)
                    gAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGaac                                                                                 <  2:1749267/100‑1 (MQ=255)
                    gAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGaac                                                                                 <  1:2838279/100‑1 (MQ=255)
                    gAATAGAAGTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGaac                                                                                 <  2:2752614/100‑1 (MQ=255)
                     aaTAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGaaca                                                                                >  2:1941984/1‑100 (MQ=255)
                      aTAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGaacaa                                                                               >  2:468027/1‑100 (MQ=255)
                      aTAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGaacaa                                                                               >  2:1511707/1‑100 (MQ=255)
                      aTAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGCGaacaa                                                                               >  2:1667527/1‑100 (MQ=255)
                        aGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAAcc                                                                             <  2:169815/100‑1 (MQ=255)
                        aGAATTACCGCCAGCCGCCGAAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGaacaac                                                                              <  1:144460/99‑1 (MQ=255)
                         gAATTACCGCGAGCCGCCGCAATCATGTAGTTAATAGCGGGGATCTATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACcg                                                                            <  2:2475314/100‑1 (MQ=255)
                         gAATTACCGCCAGCTGCCGCCATCATGTAGAGAATTGCGGGGATAGATCGGTTGAGACTGGAAAATGCCGTGCAATGAACGGCATGCTTGTGaaaaa                                                                               >  1:2026516/1‑97 (MQ=255)
                           aTTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACcgcg                                                                          <  2:1448185/100‑1 (MQ=255)
                           aTTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACcgcg                                                                          >  1:1798783/1‑100 (MQ=255)
                             tACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGtt                                                                        >  2:1831676/1‑100 (MQ=255)
                              aCCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTg                                                                       >  1:2372692/1‑100 (MQ=255)
                              aCCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTg                                                                       >  2:286744/1‑100 (MQ=255)
                              aCCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAAGGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTg                                                                       <  2:818453/100‑1 (MQ=255)
                               ccGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTgg                                                                      >  1:1603662/1‑100 (MQ=255)
                                    aGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATcc                                                                 <  2:2045231/100‑1 (MQ=255)
                                      ccgccgCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCgg                                                               >  2:2651219/1‑100 (MQ=255)
                                         ccgcAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCt                                                            >  2:195379/1‑100 (MQ=255)
                                          cgcAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTg                                                           <  1:59786/100‑1 (MQ=255)
                                           gcAATCATGTAGTGAATAGCGGTGATCGATCCGTTAATAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGc                                                          <  1:1965556/100‑1 (MQ=255)
                                           gcAATCATGTAGTGAATAGCGGTGATCGATCCGTTAACAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGc                                                          <  1:1965030/100‑1 (MQ=255)
                                           gcAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGc                                                          <  1:348646/100‑1 (MQ=255)
                                                cATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGa                                                     <  1:205311/100‑1 (MQ=255)
                                                cATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGa                                                     <  2:250626/100‑1 (MQ=255)
                                                 aTGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGAt                                                    >  2:765765/1‑100 (MQ=255)
                                                  tGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATa                                                   >  1:1496259/1‑100 (MQ=255)
                                                    tAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAgg                                                 >  1:424914/1‑100 (MQ=255)
                                                     aGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATTATTGTGAACACCCGCGTTGGCATCCGGTCTGCTTTGATAgac                                                <  2:1970544/100‑3 (MQ=255)
                                                       tGAGTAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTg                                              <  1:43482/100‑1 (MQ=255)
                                                       tGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTg                                              <  2:1338541/100‑1 (MQ=255)
                                                        gAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGc                                             <  1:1655282/100‑1 (MQ=255)
                                                        gAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGc                                             <  2:225347/100‑1 (MQ=255)
                                                        gAACAGCGGGGCTCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGc                                             <  2:2734006/100‑1 (MQ=255)
                                                          aTAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGTCATCCGGTCTGCTTTGATAGGCTGCCa                                           <  1:2454141/100‑1 (MQ=255)
                                                              cGGGGATCGATCGGTTAAGAAAGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATg                                       >  1:590682/1‑100 (MQ=255)
                                                               ggggATCGATCGGTTAAGAATGGAGACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATgg                                      <  2:833465/100‑1 (MQ=255)
                                                               ggggATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATgg                                      >  2:1505501/1‑100 (MQ=255)
                                                                gggATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGa                                     <  1:2387201/100‑1 (MQ=255)
                                                                        tcgGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCAt                             <  1:373772/100‑1 (MQ=255)
                                                                         cgGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCAtt                            >  2:1891080/1‑100 (MQ=255)
                                                                         cgGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGAAAACGCCAtt                            >  1:1444245/1‑100 (MQ=255)
                                                                         cgGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAAAAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGcc                               >  1:1612306/1‑97 (MQ=255)
                                                                          ggTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTc                           >  2:225410/1‑100 (MQ=255)
                                                                            ttAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGGGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGc                         <  1:58201/100‑1 (MQ=255)
                                                                            ttAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGc                         <  1:2195559/100‑1 (MQ=255)
                                                                             tAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCt                        <  2:1644843/100‑1 (MQ=255)
                                                                             gatctaTGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCt                        <  1:1007416/95‑1 (MQ=255)
                                                                              aaGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCtt                       <  2:2121858/100‑1 (MQ=255)
                                                                               agaatccacaCTGCCGTGCCATGAACGTCATGTTTGTGCACAACCGCGTTGGCACTCGGTCTGCTTTGATAGGCTGCCATATAGAAACGCCATTCGCTTg                      <  1:2696878/91‑1 (MQ=255)
                                                                               aGAATGGAAACTGCCGTGCAGTGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTg                      >  1:1776409/1‑100 (MQ=255)
                                                                                 aaTGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAg                    <  2:28114/100‑1 (MQ=255)
                                                                                  aTGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCtagatc                   >  2:1007419/1‑95 (MQ=255)
                                                                                  aTGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAgg                   >  2:393907/1‑100 (MQ=255)
                                                                                   tGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAggg                  <  1:1763633/100‑1 (MQ=255)
                                                                                    ggAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAgggg                 >  2:1233112/1‑100 (MQ=255)
                                                                                      atcatGCCGTTCATTGCACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAATCGCCATTCGCTTGAGGGGAg               >  1:521026/5‑100 (MQ=255)
                                                                                      atcatGCCGTTCATTGCACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAg               >  2:533769/5‑100 (MQ=255)
                                                                                       aaCTGCCGTGCAATGAACGGCATGATTGTGAACATCCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGc              <  1:1535817/100‑1 (MQ=255)
                                                                                       aaCTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGc              <  2:1798791/100‑1 (MQ=255)
                                                                                       aaCTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGc              <  2:424916/100‑1 (MQ=255)
                                                                                       aaCTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGc              <  2:2894100/100‑1 (MQ=255)
                                                                                         cTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCtt            <  2:710061/100‑1 (MQ=255)
                                                                                           gCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTttc          <  2:587335/100‑1 (MQ=255)
                                                                                                gCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTttccttcc     >  1:909058/1‑100 (MQ=255)
                                                                                                  aaTGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTc   <  1:2205633/100‑1 (MQ=255)
                                                                                                  aaTGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTc   <  2:1017437/100‑1 (MQ=255)
                                                                                                  aaTGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTc   <  1:2584156/100‑1 (MQ=255)
                                                                                                   aTGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTCa  <  1:827620/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGTCGCATAAGGCGCGCAAGGAATAGAATTACCGCCAGCCGCCGCAATCATGTAGTGAATAGCGGGGATCGATCGGTTAAGAATGGAAACTGCCGTGCAATGAACGGCATGATTGTGAACAACCGCGTTGGCATCCGGTCTGCTTTGATAGGCTGCCATATGGAAACGCCATTCGCTTGAGGGGAGCTTTCCTTCCTCA  >  NC_000913/2933299‑2933497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: