Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,967,887 G→A 100% A183V (GCC→GTC)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,967,8870GA89.5% 295.3 / 20.7 114A183V (GCC→GTC) ptsPPEP‑protein phosphotransferase enzyme I; GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base G (5/7);  new base A (47/55);  total (52/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 115 total aligned reads displayed.

AGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCT  >  NC_000913/2967788‑2967982
                                                                                                   |                                                                                               
aGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCgg                                                                                                 <  1:2787470/100‑1 (MQ=255)
 gcgcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGc                                                                                                <  2:2579764/100‑1 (MQ=255)
 gcgcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGc                                                                                                <  2:1516000/100‑1 (MQ=255)
    ncccGGTCAGTCGTTCGCATTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTTCCAGCCTCCGACAAt                                                                                             >  1:266295/2‑100 (MQ=255)
    ccccGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAAt                                                                                             >  1:2068547/1‑100 (MQ=255)
     cccGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATc                                                                                            >  2:1509724/1‑100 (MQ=255)
      ccGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCg                                                                                           <  2:363240/100‑1 (MQ=255)
      ccGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCACCCTTCGGCAAtgg                                                                                           <  2:1979211/100‑3 (MQ=255)
        ggTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGcc                                                                                         <  1:280077/100‑1 (MQ=255)
           cAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCcaca                                                                                      >  1:393480/1‑100 (MQ=255)
           cAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCAATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCcaca                                                                                      <  2:1617328/100‑1 (MQ=255)
                 ttCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGt                                                                                <  2:922311/100‑1 (MQ=255)
                     cgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGtgctg                                                                            >  1:1944118/1‑100 (MQ=255)
                       ttCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGcc                                                                          <  2:1373289/100‑1 (MQ=255)
                       ttCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGcc                                                                          <  1:2917424/100‑1 (MQ=255)
                             agCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGTCACACCAGGTGCTGCCGGTAAt                                                                    <  1:1898529/100‑1 (MQ=255)
                              gCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTa                                                                      <  1:1009602/97‑1 (MQ=255)
                              gCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAAt                                                                    <  1:1374929/99‑1 (MQ=255)
                                cGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATgcg                                                                 >  1:1767300/1‑100 (MQ=255)
                                cGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATgcg                                                                 >  2:2020793/1‑100 (MQ=255)
                                cGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATgcg                                                                 <  2:890373/100‑1 (MQ=255)
                                 ggATTCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGGAACGTGGCATCCTGCCAGCCGTCGACAATCGCCACACTAGGGGCGCCCGGTAATgggc                                                                >  1:2529004/1‑100 (MQ=255)
                                  gATCCATCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGcgcg                                                               <  2:593765/100‑1 (MQ=255)
                                  gATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGcgcg                                                               <  1:53261/100‑1 (MQ=255)
                                  gATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGcgcg                                                               <  2:1249502/100‑1 (MQ=255)
                                  gATCCAGCGTTGATGCATGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCTGGTGCTGCCGGTAATGcgcg                                                               <  1:1641566/100‑1 (MQ=255)
                                    tCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGa                                                             >  1:2435884/1‑100 (MQ=255)
                                     ccAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGAt                                                            >  2:742080/1‑100 (MQ=255)
                                       aGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTc                                                          <  2:1340180/100‑1 (MQ=255)
                                        gCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTAGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTcg                                                         >  1:2048681/1‑100 (MQ=255)
                                         cGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTcgc                                                        >  1:1824723/1‑100 (MQ=255)
                                         cGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTcg                                                         <  2:23500/99‑1 (MQ=255)
                                          gTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTcgcg                                                       <  1:1348994/100‑1 (MQ=255)
                                            tGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTc                                                     >  2:487376/1‑100 (MQ=255)
                                            tGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTc                                                     >  1:2353287/1‑100 (MQ=255)
                                              aTGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTTGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTg                                                   >  1:861455/1‑100 (MQ=255)
                                              aTGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCTACACCAGGTGCTGCCGGCAATGCGCGGATTcgcg                                                       >  1:925951/1‑96 (MQ=255)
                                                gCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGc                                                 <  2:161664/100‑1 (MQ=255)
                                                gCCTGATACACCGCTTCCATTAAAGGTAACGTGGCAGCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGACTCGAGTCTGGc                                                 <  1:1530408/100‑1 (MQ=255)
                                                  cTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGa                                               >  1:362062/1‑100 (MQ=255)
                                                  cTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCg                                                <  1:11589/99‑1 (MQ=255)
                                                  cTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGa                                               <  1:490684/100‑1 (MQ=255)
                                                    gATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGata                                             <  1:1509718/100‑1 (MQ=255)
                                                      tACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATAtt                                           >  1:311111/1‑100 (MQ=255)
                                                        cacCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGc                                         >  1:2638990/1‑100 (MQ=255)
                                                        cacCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGc                                         >  2:1379195/1‑100 (MQ=255)
                                                        cacCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGc                                         <  1:2398933/100‑1 (MQ=255)
                                                        cacCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGc                                         <  1:936180/100‑1 (MQ=255)
                                                          ccTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGccc                                       >  2:2649919/1‑100 (MQ=255)
                                                           ctgccccATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACGATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGc                                         <  1:652328/92‑1 (MQ=255)
                                                           cTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCa                                      >  1:1040335/1‑100 (MQ=255)
                                                           cTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCa                                      >  2:1741903/1‑100 (MQ=255)
                                                            tGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCaa                                     >  1:1450200/1‑100 (MQ=255)
                                                               tCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACa                                  >  2:1441925/1‑100 (MQ=255)
                                                                  aTTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGTGATATTGCCCAAACAAgg                               >  2:2562676/1‑100 (MQ=255)
                                                                  aTTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAgg                               >  1:861752/1‑100 (MQ=255)
                                                                  aTTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAgg                               >  2:1764391/1‑100 (MQ=255)
                                                                  aTTAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAgg                               <  1:2112694/100‑1 (MQ=255)
                                                                   ttAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTTCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGc                              <  1:2213063/100‑1 (MQ=255)
                                                                   ttAAAGGTAACGTGGCATCCTGCCAGCCTTCGACACTCGCCACACCTGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGTCGATATTGCCCAAACAAGGc                              <  1:318238/100‑1 (MQ=255)
                                                                   ttAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGc                              <  1:930893/100‑1 (MQ=255)
                                                                   ttAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGc                              >  1:1656785/1‑100 (MQ=255)
                                                                   ttAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGc                              <  1:1409918/100‑1 (MQ=255)
                                                                   ttAAAGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGc                              <  2:1455880/100‑1 (MQ=255)
                                                                     aaaGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAg                            <  1:654847/100‑1 (MQ=255)
                                                                       aGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTc                          <  2:94524/100‑1 (MQ=255)
                                                                       aGGTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTc                          >  1:284233/1‑100 (MQ=255)
                                                                        ggTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCa                         >  1:1547224/1‑100 (MQ=255)
                                                                        ggTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCa                         >  2:1691259/1‑100 (MQ=255)
                                                                        ggTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCa                         >  1:488245/1‑100 (MQ=255)
                                                                        ggTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCa                         <  2:2557491/100‑1 (MQ=255)
                                                                        ggTAACGTGGCATCCTGCCAGCCGTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCa                         <  1:1568754/100‑1 (MQ=255)
                                                                         gTAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCaa                        >  2:2513913/1‑100 (MQ=255)
                                                                          tAACGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAAc                       >  1:2186862/1‑100 (MQ=255)
                                                                           aaCGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACt                      <  1:387230/100‑1 (MQ=255)
                                                                           aaCGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACt                      >  2:1883205/1‑100 (MQ=255)
                                                                           aaCGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACt                      >  1:656431/1‑100 (MQ=255)
                                                                            aCGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTg                     >  2:414571/1‑100 (MQ=255)
                                                                             cGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGc                    <  2:1938554/100‑1 (MQ=255)
                                                                             cGTGGCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGc                    <  1:714913/100‑1 (MQ=255)
                                                                                ggCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGAc                 <  1:1683611/100‑1 (MQ=255)
                                                                                ggCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGAc                 >  2:1722102/1‑100 (MQ=255)
                                                                                 gCATCCTGCCAGCCTTCGACAATCGCCACACCCGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACt                <  1:1346394/100‑1 (MQ=255)
                                                                                 gCATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACt                <  1:2611993/100‑1 (MQ=255)
                                                                                  cATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTg               <  2:2112469/100‑1 (MQ=255)
                                                                                  cATCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTg               >  1:1758301/1‑100 (MQ=255)
                                                                                    tCCTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTgag             <  2:2898901/100‑1 (MQ=255)
                                                                                     ccTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTgaga            <  2:1831437/100‑1 (MQ=255)
                                                                                      cTGCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACGGCGGCTGagaa           <  1:1524855/100‑1 (MQ=255)
                                                                                       tGCCAGCTTTCGACATTGGACGCGCCAGTTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaa           <  1:178866/99‑1 (MQ=255)
                                                                                        gCCAGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaaag         <  2:2685783/100‑1 (MQ=255)
                                                                                         ccAGCCTTCCACAATCGCCACACCACGAACTGCCGGTAATGCGCGGATCCGCGTCTGGCGATATGGCCCAAACAAGGCAGTCAACTGCTACTGagaa           <  1:1964213/97‑1 (MQ=255)
                                                                                           aGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAAt      <  1:2057203/100‑1 (MQ=255)
                                                                                           aGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAAt      <  2:255371/100‑1 (MQ=255)
                                                                                           aGCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGCCAACTGCGACTGAGAAAGAAt      <  1:280881/100‑1 (MQ=255)
                                                                                            gCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATa     <  2:166663/100‑1 (MQ=255)
                                                                                            gCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATa     <  2:565193/100‑1 (MQ=255)
                                                                                            gCCTTCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATa     >  2:2039959/1‑100 (MQ=255)
                                                                                               ttCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCt  <  2:640988/100‑1 (MQ=255)
                                                                                               ttCGACAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAAAAGCt  >  1:1281721/1‑100 (MQ=255)
                                                                                                   |                                                                                               
AGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCT  >  NC_000913/2967788‑2967982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: