Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,068,922 G→T 17.1% intergenic (‑114/+25) argO ← / ← mscS arginine transporter/mechanosensitive channel protein, small conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,068,9220GT17.1% 353.4 / 52.4 133intergenic (‑114/+25)argO/mscSarginine transporter/mechanosensitive channel protein, small conductance
Reads supporting (aligned to +/- strand):  ref base G (56/54);  new base T (13/10);  total (69/64)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 136 total aligned reads displayed.

ATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGG  >  NC_000913/3068823‑3069017
                                                                                                   |                                                                                               
aTTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCagag                                                                                                 <  1:1024016/100‑1 (MQ=255)
  tAAGTATCTGATGCAGAGAATATATCGCGGGATTGTTATTAGCAGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCagagag                                                                                               <  1:526111/100‑1 (MQ=255)
     gTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtat                                                                                            >  2:2674660/1‑100 (MQ=255)
       aTCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAGATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                          >  1:1593805/1‑100 (MQ=255)
       aTCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                          <  2:2726659/100‑1 (MQ=255)
       aTCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                          >  1:1291/1‑100 (MQ=255)
           gATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAATATTATCTg                                                                                      >  1:1409235/1‑100 (MQ=255)
             tGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAATTAATAAGAGAGAGGTCAGAGAGTATTATCTgg                                                                                     >  1:9229/1‑99 (MQ=255)
             tGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGc                                                                                    >  1:1228713/1‑100 (MQ=255)
              gCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGcc                                                                                   >  2:2024089/1‑100 (MQ=255)
               cAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCt                                                                                  <  1:114414/100‑1 (MQ=255)
               cAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCt                                                                                  >  2:477489/1‑100 (MQ=255)
                agagACTATATCGAGGGATTGTTATTAGCGGAAATTGAGAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTc                                                                                 >  1:1796536/1‑100 (MQ=255)
                agagACTAGATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTc                                                                                 <  1:914950/100‑1 (MQ=38)
                 gagaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTct                                                                                <  1:776093/100‑2 (MQ=38)
                 gagaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTct                                                                                <  1:16570/100‑2 (MQ=38)
                  agaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTctc                                                                               <  2:1461748/100‑3 (MQ=38)
                   gaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGc                                                                              >  1:210677/1‑100 (MQ=255)
                    aCTATATCGCGGGATTGTTATTAGCGAAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTctctc                                                                             <  1:2899926/100‑5 (MQ=37)
                      tataTCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGtt                                                                           <  2:1272101/100‑1 (MQ=255)
                      tataTCGCGGGATTGTTATTAGCGGAAATTGATAATATTAACTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTctctctt                                                                           <  1:2649519/100‑7 (MQ=37)
                        tatCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGa                                                                         >  2:954821/1‑100 (MQ=255)
                        tatCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGa                                                                         >  1:2276255/1‑100 (MQ=255)
                         atCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGAt                                                                        >  2:368527/1‑100 (MQ=255)
                          tCGCGGGATTCTTAATAGCGGAAATTGATAATATTAATGGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGAtt                                                                       <  1:2536428/100‑1 (MQ=255)
                           cgcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCATAGAGTATTATCTGGCCTCAGCGTTGATTa                                                                      >  1:290121/1‑100 (MQ=255)
                           cgcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTa                                                                      >  1:1050073/1‑100 (MQ=255)
                            gcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTAc                                                                     >  2:527408/1‑100 (MQ=255)
                            gcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAGAAAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACg                                                                    >  1:818569/1‑100 (MQ=255)
                              gggATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGc                                                                   <  1:1350687/100‑1 (MQ=255)
                               ggATTGTTATTAGCGCAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCa                                                                  <  2:2875437/100‑1 (MQ=255)
                                    gTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCttt                                                             >  1:2041103/1‑100 (MQ=255)
                                     ttattaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTg                                                            <  2:2815033/100‑1 (MQ=255)
                                     ttattaGCGGAAATTGATAATAGTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTg                                                            <  1:1690700/100‑1 (MQ=255)
                                      tattaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGt                                                           >  1:1012779/1‑100 (MQ=255)
                                       attaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtc                                                          <  1:2674644/100‑1 (MQ=255)
                                         taGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtctt                                                        >  1:227071/1‑100 (MQ=255)
                                         taGAGGAAATGGATAATATTAATTGTCAATCATAAAAACGAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtct                                                         <  1:1538388/99‑1 (MQ=255)
                                                aaTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAc                                                 <  2:2161103/100‑1 (MQ=255)
                                                 aTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAcc                                                <  1:6247/100‑1 (MQ=255)
                                                 aTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAcc                                                <  2:2578119/100‑1 (MQ=255)
                                                     ataataTTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCt                                            >  1:1190685/1‑100 (MQ=255)
                                                     ataataTTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATATGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCt                                            <  1:1371764/100‑1 (MQ=255)
                                                     ataataTTAATAGGCAATAAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCt                                            <  1:742504/100‑1 (MQ=255)
                                                         tatTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTaaa                                        >  1:2096276/1‑100 (MQ=255)
                                                          atTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAg                                       <  1:2489659/100‑1 (MQ=255)
                                                          atTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAg                                       >  2:902343/1‑100 (MQ=255)
                                                           ttAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGt                                      <  2:2253517/100‑1 (MQ=255)
                                                           ttAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGt                                      <  1:182569/100‑1 (MQ=255)
                                                            tAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGtt                                     <  1:1450280/100‑1 (MQ=255)
                                                            tAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGtt                                     <  2:326897/100‑1 (MQ=255)
                                                              aTTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTca                                   <  2:2371003/100‑1 (MQ=255)
                                                              aTTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTca                                   <  2:192078/100‑1 (MQ=255)
                                                                  gCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATc                               >  1:462120/1‑100 (MQ=255)
                                                                   cAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATcc                              >  1:2195558/1‑100 (MQ=255)
                                                                   cAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATcc                              <  1:2448855/100‑1 (MQ=255)
                                                                   cAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTAACATc                               >  1:2633742/1‑99 (MQ=255)
                                                                    aaTCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCa                             >  2:257984/1‑100 (MQ=255)
                                                                        aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTACAGTTCACATCCCttt                          >  1:531575/1‑99 (MQ=255)
                                                                        aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                         >  1:1637729/1‑100 (MQ=255)
                                                                        aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                         >  2:2529726/1‑100 (MQ=255)
                                                                        aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                         >  1:109941/1‑100 (MQ=255)
                                                                        aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                         >  2:174093/1‑100 (MQ=255)
                                                                         gAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGATCACATCCATTTg                         >  1:542988/1‑99 (MQ=255)
                                                                          aaaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCg                       <  2:420005/100‑1 (MQ=255)
                                                                          aaaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCg                       <  1:2265732/100‑1 (MQ=255)
                                                                           aaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCgg                      >  1:2628979/1‑100 (MQ=255)
                                                                           aaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCgg                      <  1:350824/100‑1 (MQ=255)
                                                                              aCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTa                   <  2:2633758/100‑1 (MQ=255)
                                                                               cTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTAc                  <  2:1668374/100‑1 (MQ=255)
                                                                                 aataaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTAc                  >  1:802057/1‑98 (MQ=255)
                                                                                 aataaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACgg                <  1:2447038/100‑1 (MQ=255)
                                                                                 aataaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACgg                >  2:2537494/1‑100 (MQ=255)
                                                                                   taaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGa              <  2:2597521/100‑1 (MQ=255)
                                                                                   taaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGa              >  2:1261729/1‑100 (MQ=255)
                                                                                     agagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAg            >  2:1408316/1‑100 (MQ=255)
                                                                                      gagagagTCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCCGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc           >  1:1601533/1‑100 (MQ=255)
                                                                                      gagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc           <  1:1148764/100‑1 (MQ=255)
                                                                                      gagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc           <  2:1807480/100‑1 (MQ=255)
                                                                                      gagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc           <  2:2115502/100‑1 (MQ=255)
                                                                                      cgctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCTGGTACGGGAAGc           >  2:778421/5‑100 (MQ=37)
                                                                                       gctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt          >  1:445913/4‑100 (MQ=38)
                                                                                       gctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt          >  2:2559578/4‑100 (MQ=38)
                                                                                        gagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg         <  1:188530/100‑1 (MQ=255)
                                                                                        ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg         >  2:2474416/3‑100 (MQ=38)
                                                                                        ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg         >  1:2405746/3‑100 (MQ=38)
                                                                                        ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg         >  2:643524/3‑100 (MQ=38)
                                                                                        ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg         >  1:2761894/3‑100 (MQ=38)
                                                                                        ctgagGCCAGATAATACTCTATGACCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCATAAAGTTCACGTCCATTTGCGGGTACGGGAAGCTg         >  1:621073/3‑100 (MQ=255)
                                                                                         agagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGa        <  1:2706303/100‑1 (MQ=255)
                                                                                         agagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGa        <  2:285794/100‑1 (MQ=255)
                                                                                          gagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGAt       >  1:932918/1‑100 (MQ=255)
                                                                                          gagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGAt       <  1:1789967/100‑1 (MQ=255)
                                                                                           agGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATa      >  1:650302/1‑100 (MQ=255)
                                                                                            ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc     >  1:1278739/1‑100 (MQ=255)
                                                                                            ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc     >  2:65627/1‑100 (MQ=255)
                                                                                            ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc     >  1:1328553/1‑100 (MQ=255)
                                                                                            ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc     >  1:1097651/1‑100 (MQ=255)
                                                                                             gCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAcc    <  2:2380453/100‑1 (MQ=255)
                                                                                               cAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGCTACGGGACGCTGATACcgg  >  1:1131482/1‑100 (MQ=255)
                                                                                                   |                                                                                               
ATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGG  >  NC_000913/3068823‑3069017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: