Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 254,228 A→T 16.9% intergenic (+26/+31) prfH → / ← pepD pseudogene, RF‑1 domain family; probable peptide chain release factor/aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913254,2280AT16.9% 337.9 / 51.5 135intergenic (+26/+31)prfH/pepDpseudogene, RF‑1 domain family; probable peptide chain release factor/aminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (52/60);  new base T (11/12);  total (63/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

AATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTG  >  NC_000913/254130‑254327
                                                                                                  |                                                                                                   
    ttCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTaaa                                                                                                <  2:685881/100‑1 (MQ=255)
    ttCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTaaa                                                                                                >  1:1366951/1‑100 (MQ=255)
      ccATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACg                                                                                              >  1:1011325/1‑100 (MQ=255)
      ccATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACg                                                                                              <  2:1424926/100‑1 (MQ=255)
      ccATCACCAGATTGAACGTGGCAAACCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACg                                                                                              <  1:1864323/100‑1 (MQ=255)
       cATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGc                                                                                             <  2:2172242/100‑1 (MQ=255)
        aTCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGc                                                                                             <  2:876113/99‑1 (MQ=255)
           aCCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATc                                                                                         >  2:196015/1‑100 (MQ=255)
           aCCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATc                                                                                         >  1:1921365/1‑100 (MQ=255)
           aCCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATc                                                                                         >  2:455885/1‑100 (MQ=255)
              aGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCgg                                                                                      <  2:1326601/100‑1 (MQ=38)
              aGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCgg                                                                                      <  1:1852036/100‑1 (MQ=38)
              aGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGATTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCgg                                                                                      <  1:2049581/100‑1 (MQ=38)
               gATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCGgc                                                                                     <  2:848901/100‑1 (MQ=38)
               gATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgc                                                                                     >  2:772663/1‑100 (MQ=255)
                aTTGAACGTGGCAACCCGCGACTGACATTTACAAGCATCGCTTTTATCGAAGGCTAATGAAGCATGAAACTGCCGAATGGCGTTTAATCGCCTTCCgg                                                                                      <  1:283875/98‑1 (MQ=25)
                aTTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCGgca                                                                                    <  1:2296677/100‑1 (MQ=38)
                aTTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCGgca                                                                                    <  2:440415/100‑1 (MQ=38)
                aTTGAAAGTGGCAACCCTCGACGGAGATTTACAGGGGTCGTTTTTATCGAAGGATAATTAAGGATGAAACTGCCGGATGTCGTTTAATCGCCTTCCggt                                                                                     <  1:2052090/99‑2 (MQ=21)
                 ttGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGGAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCGgcag                                                                                   <  2:2480319/100‑1 (MQ=37)
                 ttGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCGgcag                                                                                   <  2:2400867/100‑1 (MQ=38)
                  tGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagc                                                                                  <  2:1910617/100‑1 (MQ=255)
                  tGAACGTGGCAACCCGCGACGGACATTGACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCGgcagt                                                                                  <  1:930650/100‑2 (MQ=37)
                    aaCGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGcaa                                                                                >  1:75996/1‑100 (MQ=255)
                    aaCGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGcaa                                                                                <  1:1143878/100‑1 (MQ=255)
                       gTGGCAACCCGCGACGGACATTTACAGTGATGGCTGTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCTATTAAACGCCATCCGGCAGcaaatc                                                                             <  1:1318396/100‑1 (MQ=255)
                        tGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGcaaatca                                                                            <  2:172805/100‑1 (MQ=255)
                        tGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGcaaatca                                                                            <  1:1330179/100‑1 (MQ=255)
                          gCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAGCTGCCGGATGGCGTTTAATCGCCTTCCGgcagtttcatcct                                                                          <  2:1792267/100‑10 (MQ=21)
                           cAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGcaaatcaaat                                                                         <  2:1761197/100‑1 (MQ=255)
                           cAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGcaaatcaaat                                                                         >  1:1531716/1‑100 (MQ=255)
                              cccGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat                                                                      >  2:83821/1‑100 (MQ=255)
                                 gcgACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTAc                                                                   <  1:2519759/100‑1 (MQ=255)
                                    aCGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTc                                                                <  2:1489860/100‑1 (MQ=255)
                                      ggACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGc                                                              <  2:1402261/100‑1 (MQ=255)
                                        aCATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCg                                                            <  1:2532567/100‑1 (MQ=255)
                                        aCATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCg                                                            <  2:87995/100‑1 (MQ=255)
                                           tttACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGaa                                                         >  2:1146192/1‑100 (MQ=255)
                                            ttACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAt                                                        <  1:453882/100‑1 (MQ=255)
                                              aCAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAttt                                                      >  2:1754508/1‑100 (MQ=255)
                                                 gggATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAtttct                                                    >  1:1451159/1‑99 (MQ=255)
                                                  ggATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAtttcttt                                                  >  2:280710/1‑100 (MQ=255)
                                                   gATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAtttctttc                                                 <  1:386870/100‑1 (MQ=255)
                                                   gATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAtttctttc                                                 <  2:415485/100‑1 (MQ=255)
                                                   gATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAtttctttc                                                 <  1:574854/100‑1 (MQ=255)
                                                    aTGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTca                                                <  1:2740616/100‑1 (MQ=255)
                                                     tGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcag                                               <  1:2675896/100‑1 (MQ=255)
                                                     tGGCTTTGATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcag                                               <  1:2639534/100‑1 (MQ=255)
                                                      ggCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagc                                              <  1:1037364/100‑1 (MQ=255)
                                                      ggCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagc                                              <  2:2004132/100‑1 (MQ=255)
                                                       gCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagca                                             >  2:758417/1‑100 (MQ=255)
                                                        cTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagcag                                            >  1:707150/1‑100 (MQ=255)
                                                        cTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagcag                                            >  1:1360141/1‑100 (MQ=255)
                                                        cTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagcag                                            >  1:1360327/1‑100 (MQ=255)
                                                           ttATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtc                                         <  1:531152/100‑1 (MQ=255)
                                                           ttATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtc                                         >  1:1538756/1‑100 (MQ=255)
                                                            tATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca                                        >  2:1481259/1‑100 (MQ=255)
                                                            tATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca                                        <  2:1483101/100‑1 (MQ=255)
                                                             aTCGAAGGATAATGAAGGATGAGACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcag                                       >  1:1693938/1‑100 (MQ=255)
                                                             aTCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcag                                       >  2:1476563/1‑100 (MQ=255)
                                                              tCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagt                                      >  1:784089/1‑100 (MQ=255)
                                                              tCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagt                                      >  1:2752319/1‑100 (MQ=255)
                                                              tCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagt                                      >  1:784739/1‑100 (MQ=255)
                                                                gAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagtca                                    >  2:292809/1‑100 (MQ=255)
                                                                gAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagtca                                    >  1:2058486/1‑100 (MQ=255)
                                                                    gATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTcagcag                                <  2:2790608/100‑1 (MQ=255)
                                                                      tAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAgtg                              >  1:2342463/1‑100 (MQ=255)
                                                                       aaTGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAgtgt                             >  1:2666070/1‑100 (MQ=255)
                                                                         tGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTcc                           <  1:60342/100‑1 (MQ=255)
                                                                         tGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTcc                           <  1:1443535/100‑1 (MQ=255)
                                                                         tGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTcc                           >  2:613600/1‑100 (MQ=255)
                                                                             ggATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTa                       >  2:1728414/1‑100 (MQ=255)
                                                                             ggATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTa                       >  2:1586227/1‑100 (MQ=255)
                                                                              gATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTaa                      >  2:674039/1‑100 (MQ=255)
                                                                                 gAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGa                   <  1:2552010/100‑1 (MQ=255)
                                                                                 gAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGa                   >  1:1898420/1‑100 (MQ=255)
                                                                                   aaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGAcc                 <  1:651016/100‑1 (MQ=255)
                                                                                   aaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGAcc                 <  1:1123409/100‑1 (MQ=255)
                                                                                     cTGCCGGATGGCGTTTAATCGCCTTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTa               >  2:2116354/1‑100 (MQ=38)
                                                                                      tGCCGGATGGCGTTTAATCGCCTTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTAc              >  1:1220319/1‑100 (MQ=38)
                                                                                       gCCGGATGGCGTTTAATCGCCTTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACg             >  2:2687841/1‑100 (MQ=38)
                                                                                       gCCGGATGGCGTTTAAGCGCCTTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACg             >  1:1050881/1‑100 (MQ=39)
                                                                                        ccGGATGGCGTTTAATCGCCTTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGc            >  1:1716767/1‑100 (MQ=38)
                                                                                        ccGGATGGCGTTTAATCGCCTTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGc            >  1:2813467/1‑100 (MQ=38)
                                                                                         cGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGc            >  1:1838984/1‑99 (MQ=255)
                                                                                          ggAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCtt          >  2:1672468/1‑100 (MQ=255)
                                                                                             aGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCg       <  2:2472061/100‑1 (MQ=255)
                                                                                             aGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCg       >  2:2909165/1‑100 (MQ=255)
                                                                                              ggCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGa      <  2:1884937/100‑1 (MQ=255)
                                                                                              ggCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGa      >  2:1598099/1‑100 (MQ=255)
                                                                                              ggCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGa      <  1:405566/100‑1 (MQ=255)
                                                                                               gCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAt     <  2:1552070/100‑1 (MQ=255)
                                                                                                cGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAtg    <  2:898352/100‑1 (MQ=255)
                                                                                                cGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAtg    >  2:2102270/1‑100 (MQ=255)
                                                                                                cGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAtg    <  1:778586/100‑1 (MQ=255)
                                                                                                cGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAtg    <  1:1519136/100‑1 (MQ=255)
                                                                                                cGATTAAACGCCATCCGGAAGCAAATCAAATAATTACTTCTCCGTAATGTCTTTCTGCAGTTCAGTCAGCAGTGTCCTTTAATTACCTACGCTTTCg       <  1:1719997/97‑1 (MQ=255)
                                                                                                 gATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAtg    <  1:595146/99‑1 (MQ=255)
                                                                                                  aTTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAtgtg  >  2:2045818/1‑100 (MQ=255)
                                                                                                  aTTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAtgtg  >  1:2794107/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
AATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTG  >  NC_000913/254130‑254327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: