Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,068,929 A→C 17.0% intergenic (‑121/+18) argO ← / ← mscS arginine transporter/mechanosensitive channel protein, small conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,068,9290AC17.0% 316.7 / 54.2 129intergenic (‑121/+18)argO/mscSarginine transporter/mechanosensitive channel protein, small conductance
Reads supporting (aligned to +/- strand):  ref base A (54/52);  new base C (13/9);  total (67/62)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

ATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAAT  >  NC_000913/3068830‑3069028
                                                                                                   |                                                                                                   
aTCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAGATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                     >  1:1593805/1‑100 (MQ=255)
aTCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                     >  1:1198/1‑100 (MQ=255)
aTCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                     >  1:1291/1‑100 (MQ=255)
aTCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                     <  2:2726659/100‑1 (MQ=255)
    gATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAATATTATCTg                                                                                                 >  1:1409235/1‑100 (MQ=255)
     aTGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTgg                                                                                                <  1:1141557/100‑1 (MQ=255)
      tGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAATTAATAAGAGAGAGGTCAGAGAGTATTATCTgg                                                                                                >  1:9229/1‑99 (MQ=255)
       gCAGATACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGcc                                                                                              <  1:171495/100‑1 (MQ=255)
       gCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGcc                                                                                              >  2:2024089/1‑100 (MQ=255)
        cAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCt                                                                                             >  2:477489/1‑100 (MQ=255)
         agagACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTc                                                                                            <  2:2608196/100‑1 (MQ=38)
         agagACTATATCGAGGGATTGTTATTAGCGGAAATTGAGAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTc                                                                                            >  1:1796536/1‑100 (MQ=255)
          gagaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTct                                                                                           <  1:16570/100‑2 (MQ=38)
           agaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTctc                                                                                          <  2:1461748/100‑3 (MQ=38)
            gaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGc                                                                                         >  1:210677/1‑100 (MQ=255)
              cTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTTATAAGAGAGAGGCCAGATAATACTTTCTGGCCTctctc                                                                                        <  1:1797335/99‑5 (MQ=37)
               tataTCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGtt                                                                                      <  2:842086/100‑1 (MQ=255)
               tataTCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGtt                                                                                      <  2:1272101/100‑1 (MQ=255)
               tataTCGCGGGATTGTTATTAGCGGAAATTGATAATATTAACTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTctctctt                                                                                      <  1:2649519/100‑7 (MQ=37)
                 tatCGCGGGATTTTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAAGAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGa                                                                                    <  1:927461/100‑1 (MQ=255)
                 tatCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGa                                                                                    >  1:2276255/1‑100 (MQ=255)
                   tCGCGGGATTCTTAATAGCGGAAATTGATAATATTAATGGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGAtt                                                                                  <  1:2536428/100‑1 (MQ=255)
                    cgcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCATAGAGTATTATCTGGCCTCAGCGTTGATTa                                                                                 >  1:290121/1‑100 (MQ=255)
                    cgcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTa                                                                                 <  2:1474307/100‑1 (MQ=255)
                    cgcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTa                                                                                 >  1:1050073/1‑100 (MQ=255)
                    cgcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTa                                                                                 <  2:2571288/100‑1 (MQ=255)
                     gcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAGAAAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACg                                                                               >  1:818569/1‑100 (MQ=255)
                       gggATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGc                                                                              <  1:1350687/100‑1 (MQ=255)
                        ggATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCATTGATTACGCa                                                                             >  1:380279/1‑100 (MQ=255)
                        ggATTGTTATTAGCGCAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCa                                                                             <  2:2875437/100‑1 (MQ=255)
                             gTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCttt                                                                        >  1:2041103/1‑100 (MQ=255)
                              ttattaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTg                                                                       <  2:2815033/100‑1 (MQ=255)
                              ttattaGCGGAAATTGATAATAGTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTg                                                                       <  1:1690700/100‑1 (MQ=255)
                               tattaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGt                                                                      >  1:1012779/1‑100 (MQ=255)
                                  taGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtctt                                                                   >  1:227071/1‑100 (MQ=255)
                                         aaTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAc                                                            <  2:2161103/100‑1 (MQ=255)
                                         aaTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAc                                                            >  2:2510067/1‑100 (MQ=255)
                                          aTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAcc                                                           <  2:2578119/100‑1 (MQ=255)
                                              ataataTTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCt                                                       >  1:1190685/1‑100 (MQ=255)
                                              ataataTTAATAGGCAATAAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCt                                                       <  1:742504/100‑1 (MQ=255)
                                                  tatTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTaaa                                                   >  1:2096276/1‑100 (MQ=255)
                                                   atTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAg                                                  <  1:58218/100‑1 (MQ=255)
                                                   atTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAg                                                  >  2:902343/1‑100 (MQ=255)
                                                   atTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAg                                                  <  1:2489659/100‑1 (MQ=255)
                                                    ttAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGt                                                 <  2:2253517/100‑1 (MQ=255)
                                                     tAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGtt                                                <  2:326897/100‑1 (MQ=255)
                                                     tAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGtt                                                <  1:1450280/100‑1 (MQ=255)
                                                       aTTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTca                                              <  2:2371003/100‑1 (MQ=255)
                                                       aTTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTca                                              <  2:192078/100‑1 (MQ=255)
                                                         tGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTcaca                                            >  1:1516563/1‑100 (MQ=255)
                                                         tGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTcac                                             <  1:1369687/99‑1 (MQ=255)
                                                          ggCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACAt                                           >  1:1327850/1‑100 (MQ=255)
                                                           gCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATc                                          >  1:462120/1‑100 (MQ=255)
                                                            cAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATcc                                         <  1:2448855/100‑1 (MQ=255)
                                                            cAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATcc                                         >  1:2195558/1‑100 (MQ=255)
                                                            cAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTAACATc                                          >  1:2633742/1‑99 (MQ=255)
                                                             aaTCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCa                                        >  2:257984/1‑100 (MQ=255)
                                                                cAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCAttt                                     >  1:2843932/1‑100 (MQ=255)
                                                                 aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                                    >  2:174093/1‑100 (MQ=255)
                                                                 aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                                    >  2:2529726/1‑100 (MQ=255)
                                                                 aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                                    >  1:109941/1‑100 (MQ=255)
                                                                  gAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGATCACATCCATTTg                                    >  1:542988/1‑99 (MQ=255)
                                                                   aaaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCg                                  <  1:2265732/100‑1 (MQ=255)
                                                                   aaaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCg                                  <  2:420005/100‑1 (MQ=255)
                                                                    aaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCgg                                 <  1:350824/100‑1 (MQ=255)
                                                                    aaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCgg                                 <  2:549608/100‑1 (MQ=255)
                                                                       aCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTa                              <  2:2633758/100‑1 (MQ=255)
                                                                          aataaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTAc                             >  1:802057/1‑98 (MQ=255)
                                                                          aataaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACgg                           <  1:2447038/100‑1 (MQ=255)
                                                                          aataaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACgg                           >  2:2537494/1‑100 (MQ=255)
                                                                           ataaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACggg                          >  2:392463/1‑100 (MQ=255)
                                                                            taaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGa                         >  1:506012/1‑100 (MQ=255)
                                                                             aacgctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGaa                        >  1:803171/7‑100 (MQ=37)
                                                                              agagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAg                       >  2:1408316/1‑100 (MQ=255)
                                                                              acgctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGaa                        >  1:2661695/6‑99 (MQ=37)
                                                                               gagagagTCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCCGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc                      >  1:1601533/1‑100 (MQ=255)
                                                                               gagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc                      <  1:1148764/100‑1 (MQ=255)
                                                                               gagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc                      <  2:2115502/100‑1 (MQ=255)
                                                                               gagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc                      <  2:1807480/100‑1 (MQ=255)
                                                                                agagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                     <  1:1066846/100‑1 (MQ=255)
                                                                                agagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCt                     >  1:1606597/1‑100 (MQ=255)
                                                                                 gagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg                    <  1:188530/100‑1 (MQ=255)
                                                                                 ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg                    >  1:2405746/3‑100 (MQ=38)
                                                                                 ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg                    >  1:2761894/3‑100 (MQ=38)
                                                                                 ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTg                    >  2:643524/3‑100 (MQ=38)
                                                                                 ctgagGCCAGATAATACTCTATGACCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCATAAAGTTCACGTCCATTTGCGGGTACGGGAAGCTg                    >  1:621073/3‑100 (MQ=255)
                                                                                 ctgacgCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCGTTGTCTTCTTTCACGCGCTTAAAGGTCACATCCATTTGCGGGTACGGGAAGCTg                    >  1:663587/6‑100 (MQ=255)
                                                                                  agagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGa                   <  2:285794/100‑1 (MQ=255)
                                                                                  agagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGa                   <  1:2706303/100‑1 (MQ=255)
                                                                                   gagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGAt                  >  1:932918/1‑100 (MQ=255)
                                                                                    agGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATa                 >  1:650302/1‑100 (MQ=255)
                                                                                     ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc                >  2:126774/1‑100 (MQ=255)
                                                                                     ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc                >  1:1278739/1‑100 (MQ=255)
                                                                                     ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc                >  2:65627/1‑100 (MQ=255)
                                                                                      gCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAcc               <  2:2380453/100‑1 (MQ=255)
                                                                                       ccAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGGAGCTGATACcg              >  2:1834823/1‑100 (MQ=255)
                                                                                        cAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGCTACGGGACGCTGATACcgg             >  1:1131482/1‑100 (MQ=255)
                                                                                             agTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCa        >  2:365208/1‑100 (MQ=255)
                                                                                                attatCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCa     <  2:2317087/100‑1 (MQ=255)
                                                                                                   atCTGGCCTCTGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAAt  <  1:1909158/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAAT  >  NC_000913/3068830‑3069028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: