BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,194,516	A→G	100%	K551K (AAA→AAG)	yqiK →	PHB family membrane protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,194,516	0	A	G	100.0%	360.4 / ‑6.7	105	K551K (AAA→AAG)	yqiK	PHB family membrane protein, function unknown
Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (58/44); minor base C (1/1); total (60/45)

Only 100 of 109 total aligned reads displayed.

CGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTG  >  NC_000913/3194419‑3194611

cGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGc                                                                                               <  2:449750/100‑1 (MQ=255)

 gCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCa                                                                                              >  2:1896566/1‑100 (MQ=255)

   tGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCaga                                                                                            >  1:2343388/1‑100 (MQ=255)

   tGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCaga                                                                                            <  2:1651547/100‑1 (MQ=255)

    gATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCagag                                                                                           >  1:2378638/1‑100 (MQ=255)

      ttGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTa                                                                                         >  2:2876540/1‑100 (MQ=255)

       tGACTCCTTGCTCAATGAAATTGGAGTTTCAGGCGGCTCACTGGCGGCACTGACTTCACCCTTAACCTCAACACCTCCCGTCGAAGAAAAGGCagag                                                                                           <  1:1983373/97‑1 (MQ=255)

        gACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTaaa                                                                                       >  2:645960/1‑100 (MQ=255)

          cTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAcc                                                                                     >  2:493818/1‑100 (MQ=255)

           tCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAcccc                                                                                    >  1:104643/1‑100 (MQ=255)

           tCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAccc                                                                                    <  2:2686255/100‑1 (MQ=255)

           tCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTACCGTCGAAGAAAAGGCAGAGTAAAccc                                                                                    >  1:104971/1‑100 (MQ=255)

            ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                   >  1:195444/1‑100 (MQ=255)

            ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACGCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                   >  1:1757479/1‑100 (MQ=255)

                   cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                            >  2:2314595/1‑100 (MQ=255)

                   cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTATTACAg                                                                            >  1:254216/1‑100 (MQ=255)

                    aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                           <  2:1756414/100‑1 (MQ=255)

                    aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                           <  1:473105/100‑1 (MQ=255)

                     aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                          >  1:1826233/1‑100 (MQ=255)

                     aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                          >  2:775244/1‑100 (MQ=255)

                     aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACGCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                          >  1:527364/1‑100 (MQ=255)

                      tGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaa                                                                         >  1:1095655/1‑100 (MQ=255)

                       gAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaat                                                                        <  2:611854/100‑1 (MQ=255)

                       gAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaat                                                                        <  2:614134/100‑1 (MQ=255)

                        aaaTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaata                                                                       >  2:1267450/1‑100 (MQ=255)

                           ttGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATc                                                                    <  2:2293877/100‑1 (MQ=255)

                            tGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATcc                                                                   <  2:74532/100‑1 (MQ=255)

                              gCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCt                                                                 <  2:1326643/100‑1 (MQ=255)

                                gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTgg                                                               >  2:1816579/1‑100 (MQ=255)

                                gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTgg                                                               >  1:614179/1‑100 (MQ=255)

                                gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTgg                                                               <  2:1031754/100‑1 (MQ=255)

                                gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTgg                                                               >  1:510845/1‑100 (MQ=255)

                                gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTgg                                                               <  1:320658/100‑1 (MQ=255)

                                 tttCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGc                                                              <  2:2109622/100‑1 (MQ=255)

                                  ttCAGGCGGCGCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGcc                                                             <  1:2224289/100‑1 (MQ=255)

                                    cAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCa                                                           <  1:1174701/100‑1 (MQ=255)

                                    cAGGCGGCTCACTGGCGGCATTGACCTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAACCTAATCCAGAAATACTCCCTgacaca                                                           >  1:2022210/1‑95 (MQ=255)

                                     aGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCaa                                                          >  2:2342008/1‑100 (MQ=255)

                                      ggcgtcTCACTGTCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCaaa                                                         <  1:2663989/100‑1 (MQ=255)

                                      ggcggcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCaaa                                                         <  1:493815/100‑1 (MQ=255)

                                         ggcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCAAGGAATAATCCCTGGCCCAAAAAg                                                      >  1:559084/1‑100 (MQ=255)

                                          gcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGc                                                     >  2:1425221/1‑100 (MQ=255)

                                           cTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGcc                                                    >  2:922122/1‑100 (MQ=255)

                                             tccgatctgcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                  <  2:2857444/92‑1 (MQ=255)

                                             cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                  <  2:417942/100‑1 (MQ=255)

                                              aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCggg                                                 <  1:2161890/100‑1 (MQ=255)

                                                  gcggcATTGACTTCACCCTTAACCTCACCAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAt                                              <  1:1388378/99‑1 (MQ=255)

                                                  gcggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtt                                             >  2:1872502/1‑100 (MQ=255)

                                                     gcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt                                          <  2:2349757/100‑1 (MQ=255)

                                                     gcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt                                          >  1:1596619/1‑100 (MQ=255)

                                                     gcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGAGAtcgga                                          >  1:2857425/1‑96 (MQ=255)

                                                      cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                         >  2:2573549/1‑100 (MQ=255)

                                                      cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                         >  2:1483628/1‑100 (MQ=255)

                                                      cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                         <  2:762096/100‑1 (MQ=255)

                                                      cATTGACTTCACCCTTAACCGCAACAACCCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                         <  2:1310064/100‑1 (MQ=255)

                                                        ttGACTTCACCCGTAACCTCAACAGCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtat                                       <  2:166165/100‑1 (MQ=255)

                                                        ctGACTTCCACCTCAACCTACACAAATCCCGCCTGAGACAACACACAGTAAACCCTCTTCCGTGAATAATCCCTTGCCCAAAAAGCCGGGAAtattg                                          >  1:537902/2‑96 (MQ=255)

                                                         tGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtata                                      >  1:2273984/1‑100 (MQ=255)

                                                          gACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtat                                       >  1:562172/1‑98 (MQ=255)

                                                           aCTTCACCCTTAACCTCAACAACCCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATc                                    <  2:2118430/100‑1 (MQ=255)

                                                            catCACCCTTAACATCAACAACTCCCGTCGAAGAAAAGGCAGCGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCt                                   >  2:1966308/3‑100 (MQ=38)

                                                            cTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCt                                   >  2:689477/1‑100 (MQ=255)

                                                               naCCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCtac                                 >  2:1207236/2‑97 (MQ=255)

                                                                aCCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGt                               >  1:616919/1‑100 (MQ=255)

                                                                aCCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGt                               >  2:191565/1‑100 (MQ=255)

                                                                 cccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGtt                              <  1:2131863/100‑1 (MQ=255)

                                                                 cccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGtt                              >  2:1690258/1‑100 (MQ=255)

                                                                  ccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAACAAGCCGGGGATTTATTATATCTGCATTc                             >  2:2433651/1‑100 (MQ=255)

                                                                     tAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGc                          <  1:1759910/100‑1 (MQ=255)

                                                                     tAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGc                          <  2:584507/100‑1 (MQ=255)

                                                                     tAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGc                          >  1:2074419/1‑100 (MQ=255)

                                                                     tAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGc                          <  1:1790294/100‑1 (MQ=255)

                                                                     tAACCTCAACAACTACCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGTGATTTTTTATATCTGCGTTCCg                           <  1:1169123/99‑1 (MQ=255)

                                                                       aCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTa                        >  1:484815/1‑100 (MQ=255)

                                                                       aCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTa                        >  2:2692834/1‑100 (MQ=255)

                                                                        ccTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaa                       >  1:1186020/1‑100 (MQ=255)

                                                                            aacaacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAg                   <  2:2300432/100‑1 (MQ=255)

                                                                             acaacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGt                  >  1:1169769/1‑100 (MQ=255)

                                                                              caacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTg                 >  2:1099756/1‑100 (MQ=255)

                                                                               aacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc                <  1:1088238/100‑1 (MQ=255)

                                                                               aacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc                <  1:670131/100‑1 (MQ=255)

                                                                               aacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc                <  2:1322235/100‑1 (MQ=255)

                                                                                 cTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc                <  2:2400409/98‑1 (MQ=255)

                                                                                 cTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaa              >  2:1227240/1‑100 (MQ=255)

                                                                                 cTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaa              <  1:1357873/100‑1 (MQ=255)

                                                                                  tCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaaa             >  1:1099121/1‑100 (MQ=255)

                                                                                        cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa       >  2:2522793/1‑100 (MQ=255)

                                                                                        cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa       >  2:1557816/1‑100 (MQ=255)

                                                                                        cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa       >  2:169729/1‑100 (MQ=255)

                                                                                        cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa       >  2:2835537/1‑100 (MQ=255)

                                                                                         gaagaaAAGGCAGAGTAAACCCTCTTCCAGTAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg      <  1:497865/100‑1 (MQ=255)

                                                                                         gaagaaAAGGCAGAGTAAACCCTCTACGAGGAATCATCCCTGGCGCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg      <  1:2509750/100‑1 (MQ=21)

                                                                                          aagtaAACGCAGAGTAAACCCTCTTCCATGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCGGCTAAAAATTGCAAATGATctg      <  1:1804950/99‑3 (MQ=21)

                                                                                           agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt    <  1:2434015/100‑1 (MQ=255)

                                                                                           agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt    >  2:1164295/1‑100 (MQ=255)

                                                                                            taaaagGCAGAGAAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAggac   <  1:883462/99‑3 (MQ=17)

                                                                                            gaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt   >  2:2115076/1‑100 (MQ=255)

                                                                                             aaaagGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAAtagatcggaag  >  1:1846464/1‑90 (MQ=18)

                                                                                             aaaagGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGCTCAggac   >  2:883465/1‑97 (MQ=255)

                                                                                             aaaagGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttg  >  2:1803231/1‑100 (MQ=255)

CGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTG  >  NC_000913/3194419‑3194611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`