Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,249,628	G→A	100%	G85D (GGC→GAC)	yqjD →	membrane‑anchored ribosome‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,249,628	0	G	A	85.1%	225.0 / 29.7	94	G85D (GGC→GAC)	yqjD	membrane‑anchored ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (6/8);  new base A (39/41);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGT  >  NC_000913/3249532‑3249723                                                                                                 |                                                                                                tccTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACAt                                                                                              <  1:1873087/99‑1 (MQ=255) gCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACAt                                                                                              >  2:2314004/1‑100 (MQ=255) gCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCATGCCGCTGAGTATGTGCGCGCACATCCGTGCCCGGGCGTGGACa                                                                                               >  1:1004018/1‑99 (MQ=255)  ccTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACAtt                                                                                             <  2:1786228/100‑1 (MQ=255)  ccTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACAta                                                                                             >  2:1873096/1‑99 (MQ=255)    tGGGTGAAACCGGTGATGCCATTCCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTgg                                                                                           <  2:2542137/100‑1 (MQ=255)         gAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTg                                                                                      <  1:2741816/100‑1 (MQ=255)          aaaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGc                                                                                     >  2:2004156/1‑100 (MQ=255)          aaaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGc                                                                                     <  2:1188585/100‑1 (MQ=255)            aCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCaa                                                                                   >  2:2090962/1‑100 (MQ=255)            aCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCaa                                                                                   <  2:293436/100‑1 (MQ=255)             ccGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAAt                                                                                  >  2:1234463/1‑100 (MQ=255)                gTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCgg                                                                               <  1:2004145/100‑1 (MQ=255)                gTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCgg                                                                               <  2:1211190/100‑1 (MQ=255)                gTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCgg                                                                               <  2:1805324/100‑1 (MQ=255)                gTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCgg                                                                               <  1:2342938/100‑1 (MQ=255)                 tGATGCCATTGCCAAACAAACCCGTGTCGCGGAGGCGCGCGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgt                                                                              <  1:2047663/100‑1 (MQ=255)                  gATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgtg                                                                             <  2:1832883/100‑1 (MQ=255)                  gATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgtg                                                                             <  2:1254433/100‑1 (MQ=255)                    taccATTGCCAAAAAAACCCGTGTCGCGGCGCCGCGTGCCGACGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTa                                                                           >  2:1967644/3‑100 (MQ=255)                    tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTa                                                                           >  1:656441/1‑100 (MQ=255)                      ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTa                                                                           >  1:566264/1‑98 (MQ=255)                      ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGt                                                                         <  2:212367/100‑1 (MQ=255)                        aTTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGc                                                                       <  1:2753036/100‑1 (MQ=255)                        aTTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGc                                                                       <  2:13711/100‑1 (MQ=255)                            ccAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCgg                                                                   <  2:143003/100‑1 (MQ=255)                            ccAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCgg                                                                   >  2:1594458/1‑100 (MQ=255)                               aacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGt                                                                >  1:2765835/1‑100 (MQ=255)                                acaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGtt                                                               >  2:1410571/1‑100 (MQ=255)                                 caaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTc                                                              >  1:932676/1‑100 (MQ=255)                                 caaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTc                                                              >  2:2622192/1‑100 (MQ=255)                                   aacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctg                                                            >  2:991874/1‑100 (MQ=255)                                    acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                           <  1:2197231/100‑1 (MQ=255)                                    acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                           <  2:810787/100‑1 (MQ=255)                                    acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                           <  1:846084/100‑1 (MQ=255)                                    acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                           <  2:2713091/100‑1 (MQ=255)                                    acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGGGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                           <  1:2733907/100‑1 (MQ=255)                                     cccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                           >  1:2787703/1‑99 (MQ=255)                                      ccGTGTCGCGGCGGCGCGTGCCGATGGGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgctg                                                         <  1:743407/100‑1 (MQ=255)                                         tgtCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcg                                                      <  1:940339/100‑1 (MQ=255)                                         tgtCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcg                                                      <  2:1019901/100‑1 (MQ=255)                                          gtCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcgc                                                     >  2:2693037/1‑100 (MQ=255)                                            cgcgGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgt                                                   <  2:2436238/100‑1 (MQ=255)                                            cgcgGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgt                                                   >  2:978258/1‑100 (MQ=255)                                            cgcgGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgt                                                   >  1:8969/1‑100 (MQ=255)                                                 cggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGTGTTCGGCGGTTGcttcggaa                                              >  1:2347233/1‑97 (MQ=255)                                                  gtttctttccGATGATTATGTGCTCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTaa                                             <  2:2728082/92‑1 (MQ=255)                                                    cgcgTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAAtt                                           >  2:951234/1‑100 (MQ=255)                                                     gcgTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTa                                          <  2:1228334/100‑1 (MQ=255)                                                     gcgTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTa                                          <  1:2622176/100‑1 (MQ=255)                                                       gTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATg                                        >  1:2617624/1‑100 (MQ=255)                                                        tGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATgg                                       >  1:53201/1‑100 (MQ=255)                                                         gCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGc                                      <  1:2274517/100‑1 (MQ=255)                                                          ccGATGAGTATGTGCGTGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCg                                     <  1:1700862/100‑1 (MQ=255)                                                          ccGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCg                                     <  2:1417391/100‑1 (MQ=255)                                                          ccGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCg                                     >  1:2421628/1‑100 (MQ=255)                                                          ccGATGAGTAGGTGCGCGACCATCCGTTCGCGGGCCTGTACCTTGGCGCTACCATCCGCGTACTGCTCGGCGTTCTGCTCTCGCGTCGCTAATTATGGCg                                     <  1:1585742/100‑1 (MQ=255)                                                           cGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCgg                                    >  2:168127/1‑100 (MQ=255)                                                           cGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATGATGGCgg                                    >  1:2854719/1‑100 (MQ=255)                                                            gATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCgg                                    >  1:1359181/1‑99 (MQ=255)                                                            gATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGa                                   >  2:2478087/1‑100 (MQ=255)                                                            gATGAGTATGTACGGGAAAATCCGTGGACGGGCGTGGACATTGGCTCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGGCGTTAATTATGGCg                                     <  1:1391166/98‑1 (MQ=255)                                                             aTGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGac                                  >  2:903618/1‑100 (MQ=255)                                                               gAGTATGTGCGCGAAAATCCGTGGACCGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGacac                                <  2:2378985/100‑1 (MQ=255)                                                                 gTATGTGCGCGAAAATCCGTGGACGGGTGTGGGCATTCGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGacac                                <  1:2127608/98‑1 (MQ=255)                                                                  tATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtca                             <  2:134599/100‑1 (MQ=255)                                                                  tATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtca                             <  1:1579847/100‑1 (MQ=255)                                                                   aTGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcat                            >  2:2729648/1‑100 (MQ=255)                                                                   aTGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcat                            >  1:1444157/1‑100 (MQ=255)                                                                   aTGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcat                            >  1:1409030/1‑100 (MQ=255)                                                                     gtgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcatca                          >  2:692333/1‑100 (MQ=255)                                                                      tgCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCAc                         >  2:922630/1‑100 (MQ=255)                                                                      tgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCAc                         >  1:940612/1‑100 (MQ=255)                                                                        cgcgAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGc                       >  2:1254698/1‑100 (MQ=255)                                                                         gcgAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGca                      >  2:95283/1‑100 (MQ=255)                                                                          cgAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGcac                     >  1:1054049/1‑100 (MQ=255)                                                                             aaaTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAg                  <  2:915855/100‑1 (MQ=255)                                                                             aaaTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAg                  >  2:1793689/1‑100 (MQ=255)                                                                              aaTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACaa                   >  1:2902194/1‑98 (MQ=255)                                                                              aaTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAgg                 >  2:287161/1‑100 (MQ=255)                                                                                    tGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCgg           <  1:903615/100‑1 (MQ=255)                                                                                    tGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCgg           <  1:1156412/100‑1 (MQ=255)                                                                                     ggACGGGCGTGGACATTGGCGCTGCAATCGTTGTAGTGCTCGCCGTTCTGCTGTCGCTCCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCgg           <  1:970824/99‑1 (MQ=255)                                                                                     ggACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGt          <  1:888371/100‑1 (MQ=255)                                                                                     ggACGGGCGTGGACAATGGCGCTGCAAGCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGt          <  1:421117/100‑1 (MQ=255)                                                                                        cGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTaaa       <  1:2692930/100‑1 (MQ=255)                                                                                        cGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTaaa       <  2:2040849/100‑1 (MQ=255)                                                                                          ggCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAg     <  2:318334/100‑1 (MQ=255)                                                                                          ggCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAg     <  2:318252/100‑1 (MQ=255)                                                                                          ggCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAg     >  2:2186325/1‑100 (MQ=255)                                                                                          ggCGTGGACATTGGCGCTGCAAACGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAg     >  1:1688906/1‑100 (MQ=255)                                                                                           gCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGc    <  2:1155012/100‑1 (MQ=255)                                                                                            cGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTTGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCg   >  1:1972686/1‑100 (MQ=255)                                                                                             gTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGt  >  1:1865418/1‑100 (MQ=255)                                                                                                 |                                                                                                GCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGT  >  NC_000913/3249532‑3249723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑