Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,298,809 A→G 82.7% V13A (GTG→GCG)  yraR ← putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,298,8090AG82.7% 283.8 / 36.1 118V13A (GTG→GCG) yraRputative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base A (11/10);  new base G (48/49);  total (59/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

CACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAA  >  NC_000913/3298715‑3298904
                                                                                              |                                                                                               
cacCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGc                                                                                            >  1:1331754/1‑100 (MQ=255)
 acCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGcc                                                                                           <  1:818289/100‑1 (MQ=255)
   cccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCGGGTGACCGCCCACCAGcccc                                                                                         <  1:1784868/100‑1 (MQ=255)
    ccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCg                                                                                        <  2:449898/100‑1 (MQ=255)
    ccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCg                                                                                        >  2:814322/1‑100 (MQ=255)
      gggCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGtt                                                                                      >  2:1824626/1‑100 (MQ=255)
       ggCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTg                                                                                     <  2:1725111/100‑1 (MQ=255)
         cATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTgcg                                                                                   <  1:1038725/100‑1 (MQ=255)
           tatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGcc                                                                                 <  1:1118860/100‑1 (MQ=255)
           tatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGcc                                                                                 <  2:770866/100‑1 (MQ=255)
           tatCGCCCAACGGACGTCGCGTCGGCGCAACAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGcc                                                                                 <  1:1108062/100‑1 (MQ=255)
           gatctcccAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGcc                                                                                 <  2:2067948/95‑1 (MQ=255)
            atCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCt                                                                                >  1:2029483/1‑100 (MQ=255)
              cGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGt                                                                              >  1:2807301/1‑100 (MQ=255)
              cGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAAACAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCCGt                                                                              >  2:112021/1‑100 (MQ=255)
               gCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTa                                                                             >  1:2486345/1‑100 (MQ=255)
                cccAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGccagatc                                                                            >  1:2067936/1‑95 (MQ=255)
                  cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCCGCCCCGTTGCGCCGGTAATc                                                                          >  1:1603894/1‑100 (MQ=255)
                  cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATc                                                                          <  1:1636089/100‑1 (MQ=255)
                  cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATc                                                                          <  1:1255240/100‑1 (MQ=255)
                  cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATc                                                                          >  2:2475606/1‑100 (MQ=255)
                  cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCAGTTGAGCCTGTAATc                                                                          >  1:2602176/1‑100 (MQ=255)
                   aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCa                                                                         <  2:1041167/100‑1 (MQ=255)
                   aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCa                                                                         >  2:1195672/1‑100 (MQ=255)
                    aCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAg                                                                        >  1:783389/1‑100 (MQ=255)
                    aCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAg                                                                        <  2:81265/100‑1 (MQ=255)
                     cGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGt                                                                       <  1:2739627/100‑1 (MQ=255)
                       gACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTAc                                                                     >  1:150485/1‑100 (MQ=255)
                        aCGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTAcc                                                                    <  2:221166/100‑1 (MQ=255)
                        aCGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTAcc                                                                    <  2:2101616/100‑1 (MQ=255)
                        aCGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTAcc                                                                    >  2:2040851/1‑100 (MQ=255)
                          gtcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTg                                                                  >  1:515664/1‑100 (MQ=255)
                            cttccgatctcagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGAc                                                                <  1:260384/90‑1 (MQ=255)
                             gcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACt                                                               <  2:1490165/100‑1 (MQ=255)
                              cgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTc                                                              <  2:2438099/100‑1 (MQ=255)
                                  ggCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCt                                                          <  2:1996006/100‑1 (MQ=255)
                                  ggCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTCGCGCCTGTAATCAGTACCTGACCCATCt                                                          >  1:494510/1‑100 (MQ=255)
                                    cgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                        >  1:1840708/1‑100 (MQ=255)
                                    cgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                        >  2:1996958/1‑100 (MQ=255)
                                    cgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                        <  2:2325613/100‑1 (MQ=255)
                                     gcagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCg                                                       <  1:604208/100‑1 (MQ=255)
                                     gcagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCg                                                       >  1:2244336/1‑100 (MQ=255)
                                      cagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGAcagatcggaag                                                      >  2:260385/1‑90 (MQ=255)
                                      cagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGc                                                      <  1:2793608/100‑1 (MQ=255)
                                      cagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGc                                                      <  2:559133/100‑1 (MQ=255)
                                      cagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGc                                                      <  2:2843700/100‑1 (MQ=255)
                                      cagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGc                                                      <  1:636763/100‑1 (MQ=255)
                                       agcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCt                                                     >  2:1136692/1‑100 (MQ=255)
                                         caATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTcc                                                   <  2:2533719/100‑1 (MQ=255)
                                         caATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTcc                                                   <  2:956419/100‑1 (MQ=255)
                                         caATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTcc                                                   <  2:2134853/100‑1 (MQ=255)
                                           aTGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCtt                                                 >  2:1184491/1‑100 (MQ=255)
                                              gCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTAc                                              <  2:521182/100‑1 (MQ=255)
                                               cGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGCCTCATCTTCGCTCCttt                                                >  1:779738/1‑97 (MQ=255)
                                                gTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAg                                            <  2:1670507/100‑1 (MQ=255)
                                                gTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAg                                            <  2:2639220/100‑1 (MQ=255)
                                                 ttAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCCCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAg                                            <  1:2040840/99‑1 (MQ=255)
                                                     cTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTg                                       >  2:347074/1‑100 (MQ=255)
                                                     cTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTg                                       >  1:2888815/1‑100 (MQ=255)
                                                         cGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGTGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCt                                    >  1:500385/1‑99 (MQ=255)
                                                         cGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTg                                   <  1:1053720/100‑1 (MQ=255)
                                                           gTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAAAATTGTCTGcc                                 >  2:1345793/1‑100 (MQ=255)
                                                             tCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCtt                               <  1:1369635/100‑1 (MQ=255)
                                                             tCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCCGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCtt                               <  1:2759855/100‑1 (MQ=255)
                                                               gTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgc                             <  1:1837263/100‑1 (MQ=255)
                                                               gTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgc                             <  1:1820432/100‑1 (MQ=255)
                                                               gTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgc                             >  1:1808332/1‑100 (MQ=255)
                                                                 tAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcgc                           >  1:1333356/1‑100 (MQ=255)
                                                                 tAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcgc                           >  2:1050115/1‑100 (MQ=255)
                                                                 tAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcgc                           >  1:1222924/1‑100 (MQ=255)
                                                                      aaCATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                      <  1:834959/100‑1 (MQ=255)
                                                                      aaCATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                      >  1:572172/1‑100 (MQ=255)
                                                                      aaCATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                      <  1:1391779/100‑1 (MQ=255)
                                                                      aaCATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                      <  1:1925401/100‑1 (MQ=255)
                                                                       aCATCCGCAGCAGGTGACCGGCCGCCAGCCCCGTTGCGACTGTAATCAGTACCTGACTCATCTTCGCTCCTTTAGAGAATTGTCTGGCTTGCGCTCGACg                     >  1:1984546/1‑100 (MQ=255)
                                                                        cATCCGCAGCAGGTGACCGCCCGCCAGCCCTGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACgg                    >  2:2670112/1‑100 (MQ=255)
                                                                        cATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACgg                    >  1:974939/1‑100 (MQ=255)
                                                                         aTCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                   >  1:2282805/1‑100 (MQ=255)
                                                                         aTCCGCAGCAAGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                   >  2:2806318/1‑100 (MQ=255)
                                                                          tCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCt                  <  2:2150380/100‑1 (MQ=255)
                                                                           ccGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTc                 <  1:805852/100‑1 (MQ=255)
                                                                           ccGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTc                 <  2:2528398/100‑1 (MQ=255)
                                                                             gcagcaGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt               >  1:1460156/1‑100 (MQ=255)
                                                                               agcagGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGc             >  1:2251279/1‑100 (MQ=255)
                                                                               agcagGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGc             <  1:638074/100‑1 (MQ=255)
                                                                               agcagGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGc             <  2:2891996/100‑1 (MQ=255)
                                                                                gcagGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGGCTCATCTTCGCTCCTTTACAGAATTGGCTGCCTTGCGCTCCACGGCTCATGCa            <  2:1052731/100‑1 (MQ=255)
                                                                                 cagGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAc           <  2:294283/100‑1 (MQ=255)
                                                                                 cagGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAc           <  2:2029494/100‑1 (MQ=255)
                                                                                  agGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAc           >  1:2141926/1‑99 (MQ=255)
                                                                                   ggTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa         >  2:2908299/1‑100 (MQ=255)
                                                                                   ggTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa         >  2:170087/1‑100 (MQ=255)
                                                                                   ggTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa         >  1:2631814/1‑100 (MQ=255)
                                                                                      gACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGc      <  1:2394041/100‑1 (MQ=255)
                                                                                      gACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGc      <  1:170086/100‑1 (MQ=255)
                                                                                        ccgcccgcCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt    >  2:1570005/1‑100 (MQ=255)
                                                                                        ccgcccgcCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt    >  2:1480034/1‑100 (MQ=255)
                                                                                        ccgcccgcCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt    >  2:137362/1‑100 (MQ=255)
                                                                                         cgcccgccAGCCCCGTTGCGCCTGTAATCAGTACCTGCCTCATGTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTa   <  1:1861779/100‑1 (MQ=255)
                                                                                          gCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTaa  >  1:705567/1‑100 (MQ=255)
                                                                                              |                                                                                               
CACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAA  >  NC_000913/3298715‑3298904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: