Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,331,987 G→A 16.4% A250V (GCG→GTG)  yhbE ← EamA family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,331,9870GA16.4% 268.2 / 40.7 116A250V (GCG→GTG) yhbEEamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base G (45/51);  new base A (17/2);  total (62/54)
Fisher's exact test for biased strand distribution p-value = 7.21e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

Only 100 of 127 total aligned reads displayed.

CCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCC  >  NC_000913/3331888‑3332086
                                                                                                   |                                                                                                   
ccTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATcg                                                                                                     >  2:1483918/1‑100 (MQ=255)
 cTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATcgc                                                                                                    >  2:2169703/1‑100 (MQ=255)
   aaaaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCgcgc                                                                                                  <  1:497875/100‑1 (MQ=255)
   aaaaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCgcgc                                                                                                  <  2:2428182/100‑1 (MQ=255)
   aaaaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCgcgc                                                                                                  >  2:2821864/1‑100 (MQ=255)
       ggTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGAGGTGAGCGCGATCATCACGCTCAc                                                                                              <  1:1934506/100‑1 (MQ=255)
       ggTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGAGGTGAGCGCGATCATCACGCTCAc                                                                                              <  1:1356597/100‑1 (MQ=255)
        gTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCAcc                                                                                             >  2:1731958/1‑100 (MQ=255)
          ttAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTg                                                                                           <  2:1413571/100‑1 (MQ=255)
            aaCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTgcg                                                                                         >  1:2420336/1‑100 (MQ=255)
            aaCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCACTCACCTgcg                                                                                         <  1:1176884/100‑1 (MQ=255)
                 cGGTCTGGCGAAGAAATCGGGCCAGGCCAGTTATAAACGATCTGAAAGAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctgcc                                                                                    <  1:1969932/100‑1 (MQ=255)
                 cGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctgcc                                                                                    <  2:942748/100‑1 (MQ=255)
                  ggTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctgcct                                                                                   >  2:2442787/1‑100 (MQ=255)
                   gTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctgcctg                                                                                  >  2:1740636/1‑100 (MQ=255)
                       ggCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAg                                                                              >  1:2292392/1‑100 (MQ=255)
                       ggCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAg                                                                              >  1:962877/1‑100 (MQ=255)
                       ggCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAg                                                                              <  2:120952/100‑1 (MQ=255)
                       ggCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAg                                                                              >  2:2377136/1‑100 (MQ=255)
                        gCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGc                                                                             <  1:2888377/100‑1 (MQ=255)
                        gCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGc                                                                             >  1:1810231/1‑100 (MQ=255)
                        gCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGc                                                                             <  1:1593945/100‑1 (MQ=255)
                         cGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCg                                                                            >  2:1788212/1‑100 (MQ=255)
                          gaagaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGa                                                                           >  1:2584130/1‑100 (MQ=255)
                           aagaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAg                                                                          <  1:1728716/100‑1 (MQ=255)
                           aagaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAg                                                                          <  2:1296126/100‑1 (MQ=255)
                            agaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGc                                                                         <  2:478146/100‑1 (MQ=255)
                             gaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGcc                                                                        >  2:1992237/1‑100 (MQ=255)
                              aaaTCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCa                                                                       <  2:2844298/100‑1 (MQ=255)
                              aaaTAGGGCCAGGCCAGTGATAAAAGTTCTGTAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCTCCTGCGCTGGCAGGCAGCgcagg                                                                        <  1:854103/99‑5 (MQ=255)
                               aaTCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCAt                                                                      >  2:832251/1‑100 (MQ=255)
                                 tCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCg                                                                    <  2:1032662/100‑1 (MQ=255)
                                   gggCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCt                                                                  >  2:766468/1‑100 (MQ=255)
                                     gccaggccagTGATAAAAGATCTGAAAAAAACAGCGTACACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTc                                                                >  1:2272294/1‑100 (MQ=255)
                                            cagTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAgg                                                         >  2:1267931/1‑100 (MQ=255)
                                               tGATAAAAGATCTCAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgcg                                                      >  2:1982167/1‑100 (MQ=255)
                                                gATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCAACTGCGCTGCCAGACAGCGAGCCATCGCTTCCGCCAg                                                          >  1:2906263/1‑95 (MQ=255)
                                                 aTAAAAGGTCTGAAAACAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGcc                                                    <  1:2802173/100‑1 (MQ=255)
                                                 aTAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGcc                                                    >  1:1503735/1‑100 (MQ=255)
                                                 aTAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGcc                                                    >  2:1907835/1‑100 (MQ=255)
                                                 aTAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGcc                                                    <  2:1834832/100‑1 (MQ=255)
                                                 aTAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGcc                                                    <  1:9964/100‑1 (MQ=255)
                                                  tAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCa                                                   >  1:370555/1‑100 (MQ=255)
                                                  tAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCa                                                   <  1:1811505/100‑1 (MQ=255)
                                                   aaaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCat                                                  >  2:553202/1‑100 (MQ=255)
                                                   aaaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCat                                                  >  2:2243771/1‑100 (MQ=255)
                                                      aGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATc                                               <  1:2811273/100‑1 (MQ=255)
                                                       gATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATcc                                              <  1:1214686/100‑1 (MQ=255)
                                                          cTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTAc                                           <  1:325197/100‑1 (MQ=255)
                                                           tGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTAcc                                          >  1:2732894/1‑100 (MQ=255)
                                                           tGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTAcc                                          <  2:1567942/100‑1 (MQ=255)
                                                            gAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCAGCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTAcc                                          <  1:2370510/99‑1 (MQ=255)
                                                            gAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCa                                         >  2:2182670/1‑100 (MQ=255)
                                                            gAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCAGCGCTTCCCCCAGGGCGCCATATCCt                                             >  1:283309/1‑96 (MQ=255)
                                                             aaaaaaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCaa                                        >  1:628839/1‑100 (MQ=255)
                                                              aaaaaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAg                                       <  1:1483912/100‑1 (MQ=255)
                                                               aaaaaCAGCGTGAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAgg                                      <  1:1405438/100‑1 (MQ=255)
                                                                aaaaCAGCGTAAACAGTGGGGTGAGCGTGAGGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGt                                     <  2:1761234/100‑1 (MQ=255)
                                                                  aaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTAt                                   <  2:1329603/100‑1 (MQ=255)
                                                                  aaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTAt                                   >  2:941130/1‑100 (MQ=255)
                                                                   aCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTAtt                                  >  1:219281/1‑100 (MQ=255)
                                                                   aCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTAtt                                  <  2:2638016/100‑1 (MQ=255)
                                                                   aCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTAtt                                  >  1:1851335/1‑100 (MQ=255)
                                                                    cAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTc                                 >  2:2662059/1‑100 (MQ=255)
                                                                    cAGCGTAAACAGTGGGGTCAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTACCGCCAGGGCGCCATATCCTACCAAGGTATTc                                 <  2:209207/100‑1 (MQ=255)
                                                                      gCGTAAACAGTTGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAg                               <  2:127957/100‑1 (MQ=255)
                                                                      gCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAg                               <  1:267161/100‑1 (MQ=255)
                                                                         tAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTcc                            <  1:1893396/100‑1 (MQ=255)
                                                                         tAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTcc                            >  1:262421/1‑100 (MQ=255)
                                                                          aaaCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCTCCATATCCTACCAAGGTATTCAGTcc                            <  1:2188989/99‑1 (MQ=255)
                                                                             cAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaa                        >  2:144578/1‑100 (MQ=255)
                                                                             agcgcaggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaa                        >  1:2082572/7‑100 (MQ=38)
                                                                              gcgcaggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaa                       >  2:844806/6‑100 (MQ=38)
                                                                              aGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaa                       <  2:2245987/100‑1 (MQ=255)
                                                                               ggggggTAGGCGTGTCGATCTCGCTCACCTGCGCTGCCGGCTAGCGAGGCATCGACTCCGCGAGGGAGCCACATCCTAGCAAGTTATTCAGTcc                            <  1:2191674/92‑1 (MQ=255)
                                                                               cgcaggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaa                      >  1:803190/5‑100 (MQ=38)
                                                                               cgcaggTGAGCGCGATCATCACGCTCACCCGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaa                      >  1:166262/5‑100 (MQ=37)
                                                                                gcaggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTGTTCAGTCCGCaaaa                      >  2:2867605/4‑99 (MQ=38)
                                                                                 ggggTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGTCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaaaa                    >  1:2648350/1‑100 (MQ=255)
                                                                                 caggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaaaa                    >  2:211666/3‑100 (MQ=38)
                                                                                 caggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaaaa                    >  1:2089989/3‑100 (MQ=38)
                                                                                 caggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaaaa                    >  1:1495260/3‑100 (MQ=39)
                                                                                  aggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAAt                   >  2:1537353/2‑100 (MQ=39)
                                                                                  aggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAAt                   >  2:613578/2‑100 (MQ=39)
                                                                                   ggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAAtt                  >  1:1164598/1‑100 (MQ=255)
                                                                                   ggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAAtt                  >  2:2492212/1‑100 (MQ=255)
                                                                                      gAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTaaa               <  1:1015042/100‑1 (MQ=255)
                                                                                       aGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAAc              <  1:1267926/100‑1 (MQ=255)
                                                                                       aGCGTGATGATCGCGCTCACCTGCCCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAAc              <  2:1892477/100‑1 (MQ=255)
                                                                                         cGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACAt            >  2:391172/1‑100 (MQ=255)
                                                                                            gatgatCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCg         <  2:2601896/100‑1 (MQ=255)
                                                                                              tgatCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAg       >  2:2667759/1‑100 (MQ=255)
                                                                                              tgatCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAg       <  2:417849/100‑1 (MQ=255)
                                                                                               gatCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGc      >  2:1737963/1‑100 (MQ=255)
                                                                                                atCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCt     <  2:242939/100‑1 (MQ=255)
                                                                                                atCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCt     >  2:1580197/1‑100 (MQ=255)
                                                                                                   gcgcTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGcc  <  1:1043862/100‑1 (MQ=255)
                                                                                                   gcgcTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGcc  <  2:283320/100‑1 (MQ=255)
                                                                                                   gcgcTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGcc  <  1:1660267/100‑1 (MQ=255)
                                                                                                   gcgcTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGcc  <  2:1903826/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCC  >  NC_000913/3331888‑3332086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: