Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,395,910 T→C 38.6% T117A (ACC→GCC)  yhdP ← DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,395,9100TC38.6% 63.6 / 121.2 109T117A (ACC→GCC) yhdPDUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base T (35/32);  new base C (21/21);  total (56/53)
Fisher's exact test for biased strand distribution p-value = 8.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

Only 100 of 109 total aligned reads displayed.

GCGAAGATCGAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGTTTAACC  >  NC_000913/3395812‑3396007
                                                                                                  |                                                                                                 
gcgAAGATCGAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGCGTGTTGGTg                                                                                                  >  2:102542/1‑100 (MQ=255)
gcgAAGATCGAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTg                                                                                                  <  1:921568/100‑1 (MQ=255)
     gATCGAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTCCCACCGCTGGTGATAGGAGTGTTGGCGCGaaa                                                                                             >  1:2031870/1‑100 (MQ=255)
     gATCGAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGaaa                                                                                             <  1:1608333/100‑1 (MQ=255)
      aTCGAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGaaa                                                                                             >  1:2614429/1‑99 (MQ=255)
      aTCGAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAg                                                                                            >  2:1764956/1‑100 (MQ=255)
        cGAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAgcg                                                                                          <  2:1895097/100‑1 (MQ=255)
        cGAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAgcg                                                                                          >  2:39075/1‑100 (MQ=255)
         gAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAgcg                                                                                          <  1:1118762/99‑1 (MQ=255)
         gAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAgcgc                                                                                         >  2:1452136/1‑100 (MQ=255)
            aTGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGc                                                                                      <  2:1895680/100‑1 (MQ=255)
               gttaaaTTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTg                                                                                    <  2:2521812/96‑1 (MQ=255)
               gTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGc                                                                                   >  2:2086685/1‑100 (MQ=255)
                tCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGcc                                                                                  <  2:2792749/100‑1 (MQ=255)
                 cAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCGGCTGCCa                                                                                 <  1:1292949/100‑1 (MQ=255)
                  aaaTTGACGAAGAAACAGATCGCTGATGTGACTGGCTTGCAGACTGTCAGCACTACACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGa                                                                               <  1:1422357/100‑1 (MQ=255)
                  aaaTTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAg                                                                                >  2:1347314/1‑100 (MQ=255)
                     ttGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGaaa                                                                             <  1:2705889/100‑1 (MQ=255)
                      tGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAg                                                                            <  2:2418517/100‑1 (MQ=255)
                      tGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAg                                                                            <  2:921761/100‑1 (MQ=255)
                      tGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAg                                                                            <  1:1886132/100‑1 (MQ=255)
                       gACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGt                                                                           >  2:2523122/1‑100 (MQ=255)
                       gACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGt                                                                           >  2:2881126/1‑100 (MQ=255)
                        aCGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTg                                                                          <  2:1373761/100‑1 (MQ=255)
                        aCGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTg                                                                          <  2:1511523/100‑1 (MQ=255)
                         cGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGa                                                                         <  1:2119694/100‑1 (MQ=255)
                         cGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGa                                                                         >  2:2751864/1‑100 (MQ=255)
                         cGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGa                                                                         >  1:1402589/1‑100 (MQ=255)
                         cGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGa                                                                         >  2:1782103/1‑100 (MQ=255)
                           aagaaACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAgg                                                                       >  1:2831962/1‑100 (MQ=255)
                            agaaACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGt                                                                      <  2:2335739/100‑1 (MQ=255)
                            agaaACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGGGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAgg                                                                       >  1:1096826/1‑99 (MQ=255)
                             gaaACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGGGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTc                                                                     >  2:420048/1‑100 (MQ=255)
                              aaaCAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTcg                                                                    >  1:1503388/1‑100 (MQ=255)
                              aaaCAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTcg                                                                    >  2:38595/1‑100 (MQ=255)
                               aaCAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTcgc                                                                   >  2:2752526/1‑100 (MQ=255)
                                aCAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTcgcg                                                                  <  2:2722239/100‑1 (MQ=255)
                                 cAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGa                                                                 >  2:415398/1‑100 (MQ=255)
                                 cAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGa                                                                 >  2:1688481/1‑100 (MQ=255)
                                  aGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGaa                                                                <  2:706958/100‑1 (MQ=255)
                                      cGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGTAGTGTTGTTGCTAAATCGCAGCTTCCAGAAAGTGAGGTCGCGAAACt                                                             >  1:439806/1‑99 (MQ=255)
                                      cGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTg                                                            <  1:1061917/100‑1 (MQ=255)
                                       gCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGc                                                           <  2:1025785/100‑1 (MQ=255)
                                       gCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGc                                                           >  2:2417405/1‑100 (MQ=255)
                                        cTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGcc                                                          >  2:1085019/1‑100 (MQ=255)
                                        cTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGcc                                                          <  1:1310254/100‑1 (MQ=255)
                                          gATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAg                                                        <  1:768027/100‑1 (MQ=255)
                                          gATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAg                                                        <  1:1467807/100‑1 (MQ=255)
                                             gtgACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAgcgc                                                     <  2:2343473/100‑1 (MQ=255)
                                              tgACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCa                                                    <  2:42762/100‑1 (MQ=255)
                                              tgACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCa                                                    >  1:2225298/1‑100 (MQ=255)
                                                aCTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCata                                                  >  1:1090696/1‑100 (MQ=255)
                                                 ccggCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCatat                                                 >  2:1979266/3‑100 (MQ=255)
                                                 cTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCatat                                                 >  2:625748/1‑100 (MQ=255)
                                                 cTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCatat                                                 >  2:2890611/1‑100 (MQ=255)
                                                  tGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATg                                                <  2:2021100/100‑1 (MQ=255)
                                                  tGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGACACTGCCAGCGCATATg                                                >  2:2283452/1‑100 (MQ=255)
                                                   ggCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGt                                               >  1:262057/1‑100 (MQ=255)
                                                    gattCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTa                                              <  2:1503394/98‑1 (MQ=255)
                                                     cTTCCAGACTGTCATCACTACCACCGCTGGTGCTCGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTaa                                             >  1:2108027/1‑100 (MQ=255)
                                                      ttCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAAc                                            <  2:2688219/100‑1 (MQ=255)
                                                       tCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACa                                           >  1:2304185/1‑100 (MQ=255)
                                                             cTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGctct                                     <  2:689827/100‑1 (MQ=255)
                                                              tGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTg                                    <  2:400956/100‑1 (MQ=255)
                                                              tGTCATCACTACCACCGATGGTGATAGGAGTGTTGGTGCAAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTg                                    <  1:1449636/100‑1 (MQ=255)
                                                                tcatcaCTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGcc                                  <  1:2492581/100‑1 (MQ=255)
                                                                tcatcaCTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGcc                                  <  1:1052951/100‑1 (MQ=255)
                                                                  atcaCTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGGCAGAAAGTGCGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAg                                <  1:1571617/100‑1 (MQ=255)
                                                                  atcaCTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAg                                <  2:1339838/100‑1 (MQ=255)
                                                                   tcaCTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGa                               >  1:2885429/1‑100 (MQ=255)
                                                                   tcaCTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGa                               >  2:1757718/1‑100 (MQ=255)
                                                                   tcaCTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGa                               >  1:2008679/1‑100 (MQ=255)
                                                                    caCTACCACCGCTGGTGTTAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGAc                              <  1:752180/100‑1 (MQ=255)
                                                                    caCTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGAc                              >  1:2217323/1‑100 (MQ=255)
                                                                    caCTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGAc                              >  1:1610729/1‑100 (MQ=255)
                                                                     aCTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACa                             >  2:389925/1‑100 (MQ=255)
                                                                     aCTACCACCGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCTAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACa                             <  1:1557801/100‑1 (MQ=255)
                                                                         ccaccGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCa                         <  2:2682136/100‑1 (MQ=255)
                                                                           accGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAgc                       >  1:428124/1‑100 (MQ=255)
                                                                            ccGCTGGTGATAGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAgcg                      <  2:794359/100‑1 (MQ=255)
                                                                                tGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCag                  <  2:1096831/100‑1 (MQ=255)
                                                                                 ggTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCaga                 <  2:1568509/100‑1 (MQ=255)
                                                                                 ggTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCaga                 >  1:835244/1‑100 (MQ=255)
                                                                                    gATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTa              >  1:2368652/1‑100 (MQ=255)
                                                                                      tAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAAc            >  2:792150/1‑100 (MQ=255)
                                                                                       aGGAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAAcg           <  1:1926264/100‑1 (MQ=255)
                                                                                        ggAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAAcgc          >  2:1855783/1‑100 (MQ=255)
                                                                                        ggAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAAcgc          >  2:2917862/1‑100 (MQ=255)
                                                                                        ggAGTGTTGGTGCGAAAGCGCAGCCGACAGAAAGTGAGGTCGCGAAACTGCCAGCGCATACGTAACAGACTCTGCCAGACATCCAGCGCCGGAGTAAcgc          >  1:1906215/1‑100 (MQ=255)
                                                                                         gAGTGTTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAAcgcg         <  2:1315086/100‑1 (MQ=255)
                                                                                          aGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGt        >  2:516168/1‑100 (MQ=255)
                                                                                            tgtTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGttt      <  2:1063497/100‑1 (MQ=255)
                                                                                             gtTGGCGCGAAAGCGCAGCTGCCAGGAAGGGAGGGCGCGACACTGCGAGCGCGACTGTCAACGGCTCTACCAGTCAACCAGCGCCAGAGTAACGCGTTTa     >  1:793033/1‑100 (MQ=255)
                                                                                             gtTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGTTTa     >  1:886090/1‑100 (MQ=255)
                                                                                             gtTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGTTTa     <  1:267202/100‑1 (MQ=255)
                                                                                             gtTGGCGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGTTTa     <  1:992255/100‑1 (MQ=255)
                                                                                              ttGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGTTTaa    <  2:2777616/100‑1 (MQ=255)
                                                                                              ttGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGTTTaa    <  2:1057479/100‑1 (MQ=255)
                                                                                                ggTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGTTTAAcc  <  2:2806421/100‑1 (MQ=255)
                                                                                                ggTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGTTTAAcc  >  2:2368139/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
GCGAAGATCGAAATGGTCAAATTGACGAAGAAACAGATCGCTGATGTGACTGGCTTCCAGACTGTCATCACTACCACCGCTGGTGATAGGAGTGTTGGTGCGAAAGCGCAGCTGCCAGAAAGTGAGGTCGCGAAACTGCCAGCGCATATGTAACAGGCTCTGCCAGACATCCAGCGCCAGAGTAACGCGTTTAACC  >  NC_000913/3395812‑3396007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: