Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,407,293 T→A 18.3% intergenic (+27/‑82) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,407,2930TA18.3% 318.2 / 59.8 136intergenic (+27/‑82)accC/yhdTacetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (65/46);  new base A (17/8);  total (82/54)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 139 total aligned reads displayed.

TGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGT  >  NC_000913/3407194‑3407391
                                                                                                   |                                                                                                  
tgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAt                                                                                                    <  2:1011213/100‑1 (MQ=255)
  aCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAttt                                                                                                  >  2:1420460/1‑100 (MQ=255)
     agaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTcc                                                                                               >  1:124574/1‑100 (MQ=255)
      gaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAAGACAAATAACACTGCTAAAGCGTCAAAAGGCCGGATTTTCCg                                                                                              >  1:2046811/1‑100 (MQ=255)
         cTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGcc                                                                                           <  1:822194/100‑1 (MQ=255)
         cTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGcc                                                                                           <  2:1942391/100‑1 (MQ=255)
         cTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGcc                                                                                           >  2:1021633/1‑100 (MQ=255)
           tCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtt                                                                                         <  1:810489/100‑1 (MQ=255)
           tCCAGCATGGTGGCACTAACATCCACTATCTCGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtt                                                                                         <  1:2304801/100‑1 (MQ=255)
            ccAGCATGGTGGCACTAACATCCACTATCTGGATAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttt                                                                                        >  1:1925901/1‑100 (MQ=255)
            ccAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttt                                                                                        <  2:1181680/100‑1 (MQ=255)
            ccAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttt                                                                                        <  1:2652450/100‑1 (MQ=255)
              aGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTTGTTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttt                                                                                        <  2:829380/98‑1 (MQ=255)
              aGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttg                                                                                      <  2:1651582/100‑2 (MQ=39)
               gCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                     >  2:1274998/1‑100 (MQ=255)
               gCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                     <  1:1178888/100‑1 (MQ=255)
                 aTGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttat                                                                                   <  2:2373857/100‑1 (MQ=255)
                 aTGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttat                                                                                   <  1:920569/100‑1 (MQ=255)
                 aTGGTGGAACTAAAATCCACTATTTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttgacg                                                                                   <  1:449703/100‑5 (MQ=37)
                   ggtggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttatta                                                                                 >  2:1832034/1‑100 (MQ=255)
                    gtggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAc                                                                                >  1:16465/1‑100 (MQ=255)
                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGGATTTCCGGCCTTTTTTATTACTgg                                                                             >  1:2095438/1‑99 (MQ=255)
                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                            >  2:1503450/1‑100 (MQ=255)
                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                            >  1:2278114/1‑100 (MQ=255)
                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                            >  2:1811422/1‑100 (MQ=255)
                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                            <  2:456654/100‑1 (MQ=255)
                           tAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGAt                                                                         >  1:1026624/1‑100 (MQ=255)
                            aaCATCCACTATGTGGAGAAAAAACTCGGTCGTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGGCGGCTTTTCCGGCCTTTTTTATTACTGGGGATc                                                                        <  1:437834/100‑1 (MQ=255)
                             aCATCCACTAACTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCg                                                                       <  1:485519/100‑1 (MQ=255)
                              cATCCACTATCTGGAGAAAAAAGTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGa                                                                      >  1:1570020/1‑100 (MQ=255)
                              cATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGa                                                                      >  2:2127876/1‑100 (MQ=255)
                                    cTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAAccc                                                                <  1:1550246/100‑1 (MQ=255)
                                         tGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATa                                                           >  1:410527/1‑100 (MQ=255)
                                         tGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATa                                                           >  2:430384/1‑100 (MQ=255)
                                          ggAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATaa                                                          >  2:1127119/1‑100 (MQ=255)
                                           gagaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAg                                                         >  2:895868/1‑100 (MQ=255)
                                            agaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATaaag                                                        >  1:1677055/1‑98 (MQ=255)
                                            agaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAgg                                                        >  2:138976/1‑100 (MQ=255)
                                             gaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGt                                                       >  2:596238/1‑100 (MQ=255)
                                               aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTAc                                                     >  2:1691330/1‑100 (MQ=255)
                                               aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTAc                                                     <  2:617157/100‑1 (MQ=255)
                                                  aaCTCGGTCTTCAGGAAAAAGAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAAt                                                  <  1:1625965/100‑1 (MQ=255)
                                                   aCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTTTTTTTTTTGTTCGACAACCCCCATAAGGTACAAt                                                  >  1:1432108/1‑99 (MQ=255)
                                                     tCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATccc                                               <  2:2615745/100‑1 (MQ=255)
                                                       ggTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCg                                             <  1:1070318/100‑1 (MQ=255)
                                                       ggTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCg                                             >  2:1153736/1‑100 (MQ=255)
                                                       ggTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCg                                             >  2:2494583/1‑100 (MQ=255)
                                                         tCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCt                                           <  2:1613680/100‑1 (MQ=255)
                                                           ttCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCtt                                          <  1:910805/99‑1 (MQ=255)
                                                            tCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttc                                        >  1:577646/1‑100 (MQ=255)
                                                             cAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttct                                       <  2:424544/100‑1 (MQ=255)
                                                             cAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttct                                       >  1:2343352/1‑100 (MQ=255)
                                                              agaaaaaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttc                                        <  1:1948796/95‑1 (MQ=255)
                                                               ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                     >  2:2268582/1‑100 (MQ=255)
                                                               ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                     >  2:2230097/1‑100 (MQ=255)
                                                               ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                     >  1:2338599/1‑100 (MQ=255)
                                                               ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                     >  1:2514655/1‑100 (MQ=255)
                                                               ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                     >  1:422976/1‑100 (MQ=255)
                                                                gAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCa                                    >  2:2118468/1‑100 (MQ=255)
                                                                 aaaaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAc                                   <  2:2522788/100‑1 (MQ=255)
                                                                 aaaaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTGTTATTACCGGGGATCGACAACACCCAGAACGTACATGCCCCGCTACCTTCAc                                   >  1:2580775/1‑100 (MQ=255)
                                                                  aaaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAcc                                  <  2:1766319/100‑1 (MQ=255)
                                                                   aaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAccc                                 >  1:1715356/1‑100 (MQ=255)
                                                                   aaaTAAGACTGCTAAAGCGTCAAAAGGCCGCATCTTATGGCCTTCTTTCTCAATCCGAATCAACACTCCCCACAAGGCACAATCACGCCTCTCATCAccc                                 >  1:2906155/1‑100 (MQ=255)
                                                                    aaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAcaaa                                >  2:2431146/1‑97 (MQ=255)
                                                                    aaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCa                                >  2:2232180/1‑100 (MQ=255)
                                                                        aGACTGCTAAAGCGTCAAAAGGCCTGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAg                            <  2:1311656/100‑1 (MQ=255)
                                                                           cTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGa                         >  1:288975/1‑100 (MQ=255)
                                                                             gCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACa                       >  2:473401/1‑100 (MQ=255)
                                                                             gCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGAGATCGACAACCCCCATAAGGTACAATCCCCGTTTTCTCCACCCATCAggg                          >  1:1596079/1‑97 (MQ=255)
                                                                               tAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaa                     >  2:281073/1‑100 (MQ=255)
                                                                               tAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaa                     >  2:2785762/1‑100 (MQ=255)
                                                                                aaaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAATGGGGATCGACAACCCCCATAAGGTCCAATCTCCGCTTTCTTCACCCATCAGGGACaaaa                    >  1:1030058/1‑100 (MQ=255)
                                                                                 aaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaaa                   >  1:2808780/1‑100 (MQ=255)
                                                                                 aaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaaa                   <  2:507837/100‑1 (MQ=255)
                                                                                   gCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAAt                 <  2:1657984/100‑1 (MQ=255)
                                                                                   aataaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCAGAAGGTACAATACCCGCTTTCTTCACCCACCAGGGACGAAAAAt                 >  1:501246/6‑100 (MQ=25)
                                                                                    ataaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATg                >  2:2392193/5‑100 (MQ=38)
                                                                                    ataaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATg                >  1:236647/5‑100 (MQ=38)
                                                                                     gTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATgg               <  2:882856/100‑1 (MQ=255)
                                                                                      tCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGa              >  2:952272/1‑100 (MQ=255)
                                                                                      tCAAAAGGCCGGATTTCCCGGCAGTTTTTACTACAGGGGATCGACAACCCCCATAAGTTACAATCCCCGCTTTCTTCACCCATCAAAAACAAAAAATgg               <  1:654554/99‑1 (MQ=255)
                                                                                      aaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTCGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaacactg                >  1:815350/3‑93 (MQ=38)
                                                                                       cAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGac             <  2:864376/100‑1 (MQ=255)
                                                                                       aaaaaGGCCGGAAGATGCGGGCTTTTTGCTTACGGGAGATGGACAACACCCATAAGGTACAGTGAAGGCTTACGTGAACGATCAGGGACAAAAAAt                 >  1:1799690/2‑96 (MQ=255)
                                                                                        aaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGaca            >  2:939351/1‑100 (MQ=255)
                                                                                         aaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAACAAATGGacac           >  1:1415544/1‑100 (MQ=255)
                                                                                         aaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGacac           >  2:1096683/1‑100 (MQ=255)
                                                                                         aaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGacac           >  1:1681453/1‑100 (MQ=255)
                                                                                         aaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGacac           >  1:909410/1‑100 (MQ=255)
                                                                                            ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCg        >  1:1865542/1‑100 (MQ=255)
                                                                                            ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCg        <  2:565606/100‑1 (MQ=255)
                                                                                            ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCg        >  1:122339/1‑100 (MQ=255)
                                                                                             gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGt       >  2:818208/1‑100 (MQ=255)
                                                                                             gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGt       >  2:275226/1‑100 (MQ=255)
                                                                                              ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtt      >  1:1897685/1‑100 (MQ=255)
                                                                                              ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtt      <  1:2127863/100‑1 (MQ=255)
                                                                                               cGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGttt     <  1:2651667/100‑1 (MQ=255)
                                                                                                 gATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg   <  2:2718522/100‑1 (MQ=255)
                                                                                                 gATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg   <  2:1080911/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGT  >  NC_000913/3407194‑3407391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: