Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,407,294 T→A 17.0% intergenic (+28/‑81) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,407,2940TA17.0% 332.3 / 53.0 136intergenic (+28/‑81)accC/yhdTacetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (65/46);  new base A (15/8);  total (82/54)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 138 total aligned reads displayed.

ACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTC  >  NC_000913/3407196‑3407393
                                                                                                  |                                                                                                   
aCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAttt                                                                                                    >  2:1420460/1‑100 (MQ=255)
   agaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTcc                                                                                                 >  1:124574/1‑100 (MQ=255)
       cTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGcc                                                                                             <  1:822194/100‑1 (MQ=255)
       cTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGcc                                                                                             >  2:1021633/1‑100 (MQ=255)
         tCCAGCATGGTGGCACTAACATCCACTATCTCGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtt                                                                                           <  1:2304801/100‑1 (MQ=255)
          ccAGCATGGTGGCACTAACATCCACTATCTGGATAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttt                                                                                          >  1:1925901/1‑100 (MQ=255)
          ccAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttt                                                                                          <  1:2652450/100‑1 (MQ=255)
          ccAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttt                                                                                          <  2:1181680/100‑1 (MQ=255)
           cAGCATGGTGGGACTAACATCCATTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGc                                                                                              <  1:522221/95‑1 (MQ=255)
            aGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTTGTTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttt                                                                                          <  2:829380/98‑1 (MQ=255)
            aGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttg                                                                                        <  2:1651582/100‑2 (MQ=39)
             gCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                       >  2:1274998/1‑100 (MQ=255)
             gCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                       <  1:1178888/100‑1 (MQ=255)
              cATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTtta                                                                                      <  2:2526517/100‑1 (MQ=255)
               aTGGTGGCACTAACATCCACTATCTGGGGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttgac                                                                                      <  1:398178/99‑4 (MQ=38)
               aTGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttat                                                                                     <  2:2373857/100‑1 (MQ=255)
                 ggtggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttatta                                                                                   >  2:1832034/1‑100 (MQ=255)
                  gtggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAc                                                                                  >  1:16465/1‑100 (MQ=255)
                  gtggAACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttatta                                                                                   <  1:1993962/99‑1 (MQ=255)
                    ggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTg                                                                                <  2:1771528/100‑1 (MQ=255)
                      cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGGATTTCCGGCCTTTTTTATTACTgg                                                                               >  1:2095438/1‑99 (MQ=255)
                      cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                              >  2:1677963/1‑100 (MQ=255)
                      cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                              >  2:1811422/1‑100 (MQ=255)
                      cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                              >  1:2278114/1‑100 (MQ=255)
                         tAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGAt                                                                           >  1:1026624/1‑100 (MQ=255)
                          aaCATCCACTATGTGGAGAAAAAACTCGGTCGTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGGCGGCTTTTCCGGCCTTTTTTATTACTGGGGATc                                                                          <  1:437834/100‑1 (MQ=255)
                           aCATCCACTAACTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCg                                                                         <  1:485519/100‑1 (MQ=255)
                            cATCCACTATCTGGAGAAAAAAGTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGa                                                                        >  1:1570020/1‑100 (MQ=255)
                            cATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGa                                                                        >  2:2127876/1‑100 (MQ=255)
                                  cTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAAccc                                                                  <  1:1550246/100‑1 (MQ=255)
                                      cTGGAGAGAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCAt                                                              >  2:1532485/1‑100 (MQ=255)
                                       tGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATa                                                             >  2:430384/1‑100 (MQ=255)
                                        ggAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATaa                                                            >  2:1127119/1‑100 (MQ=255)
                                         gagaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAg                                                           >  2:895868/1‑100 (MQ=255)
                                          agaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAgg                                                          <  2:1044884/100‑1 (MQ=255)
                                          agaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAgg                                                          >  2:138976/1‑100 (MQ=255)
                                           gaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGt                                                         >  2:596238/1‑100 (MQ=255)
                                            aaaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTa                                                        <  1:904800/100‑1 (MQ=255)
                                             aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTAc                                                       >  2:1691330/1‑100 (MQ=255)
                                             aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTAc                                                       <  2:617157/100‑1 (MQ=255)
                                               aaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACaa                                                     >  1:1657977/1‑100 (MQ=255)
                                                aaCTCGGTCTTCAGGAAAAAGAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAAt                                                    <  1:1625965/100‑1 (MQ=255)
                                                 aCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTTTTTTTTTTGTTCGACAACCCCCATAAGGTACAAt                                                    >  1:1432108/1‑99 (MQ=255)
                                                  cTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATcc                                                  <  1:2812930/100‑1 (MQ=255)
                                                   tCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATccc                                                 <  2:2615745/100‑1 (MQ=255)
                                                     ggTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCg                                               >  2:1153736/1‑100 (MQ=255)
                                                     ggTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCg                                               >  2:2494583/1‑100 (MQ=255)
                                                     ggTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCg                                               <  1:1070318/100‑1 (MQ=255)
                                                       tCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGGTCGACAACCCCCATAAGGTACAATCCCCGCt                                             <  2:46491/100‑1 (MQ=255)
                                                       tCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCt                                             <  2:1613680/100‑1 (MQ=255)
                                                         ttCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCtt                                            <  1:910805/99‑1 (MQ=255)
                                                           cAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttct                                         >  1:2343352/1‑100 (MQ=255)
                                                           cAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttct                                         <  2:424544/100‑1 (MQ=255)
                                                            agaaaaaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttc                                          <  1:1948796/95‑1 (MQ=255)
                                                            aGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTTCTGGGGATCGCCAAACCCCATAAGGTACAATTCCAGCTttctt                                        >  1:2336745/1‑100 (MQ=255)
                                                            aGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttctt                                        >  2:2782166/1‑100 (MQ=255)
                                                             ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                       >  1:917122/1‑100 (MQ=255)
                                                             ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                       >  2:2230097/1‑100 (MQ=255)
                                                             ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                       >  1:422976/1‑100 (MQ=255)
                                                             ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                       >  1:2514655/1‑100 (MQ=255)
                                                              gAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCa                                      >  2:2118468/1‑100 (MQ=255)
                                                               aaaaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTGTTATTACCGGGGATCGACAACACCCAGAACGTACATGCCCCGCTACCTTCAc                                     >  1:2580775/1‑100 (MQ=255)
                                                                 aaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAccc                                   >  1:1715356/1‑100 (MQ=255)
                                                                 aaaTAAGACTGCTAAAGCGTCAAAAGGCCGCATCTTATGGCCTTCTTTCTCAATCCGAATCAACACTCCCCACAAGGCACAATCACGCCTCTCATCAccc                                   >  1:2906155/1‑100 (MQ=255)
                                                                  aaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAcaaa                                  >  2:2431146/1‑97 (MQ=255)
                                                                  aaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCa                                  >  2:2232180/1‑100 (MQ=255)
                                                                      aGACTGCTAAAGCGTCAAAAGGCCTGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAg                              <  2:1311656/100‑1 (MQ=255)
                                                                           gCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACa                         >  2:473401/1‑100 (MQ=255)
                                                                           gCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGAGATCGACAACCCCCATAAGGTACAATCCCCGTTTTCTCCACCCATCAggg                            >  1:1596079/1‑97 (MQ=255)
                                                                             tAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaa                       >  2:281073/1‑100 (MQ=255)
                                                                             tAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaa                       >  2:2785762/1‑100 (MQ=255)
                                                                              aaaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAATGGGGATCGACAACCCCCATAAGGTCCAATCTCCGCTTTCTTCACCCATCAGGGACaaaa                      >  1:1030058/1‑100 (MQ=255)
                                                                               aaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaaa                     <  2:507837/100‑1 (MQ=255)
                                                                               aaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaaa                     >  1:2808780/1‑100 (MQ=255)
                                                                                 gCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAAt                   >  2:1945131/1‑100 (MQ=255)
                                                                                 aataaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCAGAAGGTACAATACCCGCTTTCTTCACCCACCAGGGACGAAAAAt                   >  1:501246/6‑100 (MQ=25)
                                                                                  ataaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATg                  >  1:236647/5‑100 (MQ=38)
                                                                                  ataaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATg                  >  2:2392193/5‑100 (MQ=38)
                                                                                   taaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATgg                 >  1:2637637/4‑100 (MQ=38)
                                                                                   gTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATgg                 <  2:882856/100‑1 (MQ=255)
                                                                                    tCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGa                >  2:952272/1‑100 (MQ=255)
                                                                                    tCAAAAGGCCGGATTTCCCGGCAGTTTTTACTACAGGGGATCGACAACCCCCATAAGTTACAATCCCCGCTTTCTTCACCCATCAAAAACAAAAAATgg                 <  1:654554/99‑1 (MQ=255)
                                                                                    aaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATgg                 >  1:446491/3‑99 (MQ=39)
                                                                                    aaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTCGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaacactg                  >  1:815350/3‑93 (MQ=38)
                                                                                     cAAAAGGCCTGATTTTCCGGCCTTTTGTATTAATGGGGATCGACAACCCCCATAAGATACAATACCCGATTTCTTCACCCATCAGGGACAAAAAATGGac               <  1:677371/100‑1 (MQ=255)
                                                                                     cAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGac               <  2:864376/100‑1 (MQ=255)
                                                                                     aaaaaGGCCGGAAGATGCGGGCTTTTTGCTTACGGGAGATGGACAACACCCATAAGGTACAGTGAAGGCTTACGTGAACGATCAGGGACAAAAAAt                   >  1:1799690/2‑96 (MQ=255)
                                                                                      aaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGaca              >  2:939351/1‑100 (MQ=255)
                                                                                       aaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAACAAATGGacac             >  1:1415544/1‑100 (MQ=255)
                                                                                       aaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGacac             >  1:1681453/1‑100 (MQ=255)
                                                                                          ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCCGGGACAAAAAATGGACACTCg          >  1:1865553/1‑100 (MQ=255)
                                                                                          ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCg          >  1:122339/1‑100 (MQ=255)
                                                                                          ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCg          >  1:1865542/1‑100 (MQ=255)
                                                                                          ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCg          <  2:565606/100‑1 (MQ=255)
                                                                                           gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGt         >  2:818208/1‑100 (MQ=255)
                                                                                            ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtt        <  1:2127863/100‑1 (MQ=255)
                                                                                               gATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg     <  2:2718522/100‑1 (MQ=255)
                                                                                               gATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg     <  2:1080911/100‑1 (MQ=255)
                                                                                               gATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg     <  2:2343367/100‑1 (MQ=255)
                                                                                                  tttCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTc  <  2:1383849/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
ACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTC  >  NC_000913/3407196‑3407393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: