Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,468,389 T→C 15.9% S489G (AGT→GGT)  chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,468,3890TC15.9% 297.8 / 51.4 139S489G (AGT→GGT) chiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base T (60/57);  new base C (12/10);  total (72/67)
Fisher's exact test for biased strand distribution p-value = 8.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01

Only 100 of 143 total aligned reads displayed.

GCTCTTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTCGTA  >  NC_000913/3468290‑3468488
                                                                                                   |                                                                                                   
gCTCTTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACt                                                                                                     >  2:2441969/1‑100 (MQ=255)
   ctTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCCCGGGCGGCTATTGCTACCGGTGGCGTTTTGCTTCGCCACCAGCGCAGGGTTGTCACttt                                                                                                   >  1:1188071/1‑99 (MQ=255)
   ctTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTg                                                                                                  <  2:604852/100‑1 (MQ=255)
   ctTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTg                                                                                                  <  2:1841489/100‑1 (MQ=255)
     tCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCg                                                                                                <  2:1909646/100‑1 (MQ=255)
        ttGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTcc                                                                                             <  2:2230477/100‑1 (MQ=255)
         tgggcaggcttatgaagcgctgttctggacgcaaagtgacaacccTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCa                                                                                            >  1:254511‑M1/46‑100 (MQ=255)
         tGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCa                                                                                            <  1:2878113/100‑1 (MQ=255)
           cTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGa                                                                                          <  2:1032921/100‑1 (MQ=255)
            tataGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGaa                                                                                         >  1:2825617/1‑100 (MQ=255)
             ataGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAAc                                                                                        <  2:414146/100‑1 (MQ=255)
                gCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAgc                                                                                     >  2:1552459/1‑100 (MQ=255)
                gCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAgc                                                                                     <  1:2484660/100‑1 (MQ=255)
                gCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAgc                                                                                     >  1:1610608/1‑100 (MQ=255)
                gCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGCTTCGCCCCCCGAGCAGGGTTGTCACTTTGCGTCCAGAACAgc                                                                                     >  2:2514843/1‑100 (MQ=255)
                    tGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTCGTCACTTTGCGTCCAGAACAGCGCtt                                                                                 <  2:1215405/100‑1 (MQ=255)
                      aGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCa                                                                               <  1:1083233/100‑1 (MQ=255)
                       gCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCAt                                                                              >  2:1664488/1‑100 (MQ=255)
                        ccTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATa                                                                             <  2:1084114/100‑1 (MQ=255)
                         cTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATaa                                                                            <  1:1627095/100‑1 (MQ=255)
                           tACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGc                                                                          >  2:1223831/1‑100 (MQ=255)
                             ccTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCt                                                                        >  2:2719254/1‑100 (MQ=255)
                              cTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTg                                                                       >  2:1745484/1‑100 (MQ=255)
                               tAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGc                                                                      >  2:2462357/1‑100 (MQ=255)
                                aaCGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGcc                                                                     <  2:1856357/100‑1 (MQ=255)
                                  tggacgcaaagtgacaaccctgctctggtggcgaaccaaaacgccaccggtagcaGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCa                                                                   >  1:254511‑M2/56‑100 (MQ=255)
                                  cGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCa                                                                   <  2:1389531/100‑1 (MQ=255)
                                    gCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATc                                                                 <  2:1342418/100‑1 (MQ=255)
                                    gCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATc                                                                 <  1:1279207/100‑1 (MQ=255)
                                     cTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATca                                                                <  1:486895/100‑1 (MQ=255)
                                     cTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATca                                                                <  2:1523754/100‑1 (MQ=255)
                                       tCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaac                                                              <  1:2176966/100‑1 (MQ=255)
                                       tCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaac                                                              <  1:2557741/100‑1 (MQ=255)
                                         cACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaa                                                            >  2:1732778/1‑100 (MQ=255)
                                          aCGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaac                                                           <  2:2904081/100‑1 (MQ=255)
                                           cGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaaca                                                          >  2:1906492/1‑100 (MQ=255)
                                              gcggcTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAAcg                                                       >  2:2793137/1‑100 (MQ=255)
                                               cggcTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAAcgc                                                      >  1:855100/1‑100 (MQ=255)
                                                 gcTATTGCTACCGGTGGCGTTTTGGTACGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGa                                                    >  1:647632/1‑100 (MQ=255)
                                                  cTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGAt                                                   >  1:1762792/1‑100 (MQ=255)
                                                   tATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATc                                                  >  1:1279329/1‑100 (MQ=255)
                                                    cctgctctggtggcgaaccaaaacgccaccggtagcaGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCa                                                 >  2:539400‑M2/38‑100 (MQ=255)
                                                     ttGCTACCGGTGTCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCa                                                 <  1:179753/99‑1 (MQ=255)
                                                     ctgctctggtggcgaaccaaaacgccaccggtagcaGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCAc                                                >  2:592208‑M2/37‑100 (MQ=255)
                                                       tgctctggtggcgaaccaaaacgccaccggagcaGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCt                                              >  1:1333574‑M2/35‑100 (MQ=255)
                                                       gctctggtggcgaaccaaaacgcaaccggtagcaGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCACATCAACAACAACGCGATCACCt                                              >  1:708701‑M2/35‑100 (MQ=255)
                                                        CTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCtt                                             <  1:156026‑M1/100‑1 (MQ=255)
                                                         tctggtggcgaaccaaaacgccaccggtagcaGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCttt                                            >  1:452134‑M2/33‑100 (MQ=255)
                                                          agcGGTGGCGTTTTGCTTCGCCACCAGTGCAGGGTTGTCGCTTTGCGTCCAGACCAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCtt                                             <  1:2890614/96‑1 (MQ=255)
                                                          aCCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTc                                           >  1:955840/1‑100 (MQ=255)
                                                           ctggtggcgaaccaaaacgccaccagagcaGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCttt                                            >  2:2818519‑M2/31‑98 (MQ=255)
                                                           ctggtggcgaaccaaaacgccaccagagcaGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCa                                          >  2:2890538‑M2/31‑100 (MQ=255)
                                                           ccGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCa                                          <  1:78376/100‑1 (MQ=255)
                                                            cGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCAc                                         >  1:196025/1‑100 (MQ=255)
                                                             ggTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACg                                        <  2:2564378/100‑1 (MQ=255)
                                                              gtggcgaaccaaaacgccaccagagcaGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACCACAACGCGATCACCTTTCACGt                                       >  1:1165482‑M2/28‑100 (MQ=255)
                                                                ggCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTac                                     >  2:1010343/1‑100 (MQ=255)
                                                                ggCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTac                                     <  2:782102/100‑1 (MQ=255)
                                                                 gCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTaca                                    <  2:2900100/100‑1 (MQ=255)
                                                                  cGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTacac                                   >  2:701254/1‑100 (MQ=255)
                                                                   gTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTAcaca                                  >  2:629892/1‑100 (MQ=255)
                                                                   gTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTAcaca                                  <  2:742345/100‑1 (MQ=255)
                                                                    ttttGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAg                                 <  1:2006506/100‑1 (MQ=255)
                                                                     tttGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGt                                >  2:441304/1‑100 (MQ=255)
                                                                     tttGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCAAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGt                                >  2:2226209/1‑100 (MQ=255)
                                                                      ttGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTg                               <  1:1001776/100‑1 (MQ=255)
                                                                      ttGGTCCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTTCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTg                               <  1:1730425/100‑1 (MQ=255)
                                                                         gTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATACGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGAt                            >  1:1691143/1‑100 (MQ=255)
                                                                         gTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGAt                            <  2:2071217/100‑1 (MQ=255)
                                                                         gTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGAt                            >  2:200036/1‑100 (MQ=255)
                                                                           tCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGc                          >  2:2563544/1‑100 (MQ=255)
                                                                           tCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGc                          <  1:1410247/100‑1 (MQ=255)
                                                                            cGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGCACAGCGCTTCATAAGCCTGCCCATCAACAACAAAGCGATCACCTTTCACGTACCCAGTGGATGc                          >  1:1177506/1‑99 (MQ=255)
                                                                            cGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCg                         <  1:2004135/100‑1 (MQ=255)
                                                                             nccaccaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCgg                        >  2:888092/2‑100 (MQ=255)
                                                                              ccaccaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCgg                        <  1:2147366/99‑1 (MQ=255)
                                                                              ccaccaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGa                       <  1:563387/100‑1 (MQ=255)
                                                                              ccaccaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGa                       <  1:2718015/100‑1 (MQ=255)
                                                                              ccaccaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGa                       <  2:1130844/100‑1 (MQ=255)
                                                                              ccaccaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGa                       >  2:270536/1‑100 (MQ=255)
                                                                              ccaccaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGa                       >  1:2472455/1‑100 (MQ=255)
                                                                                 cggtagcaGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCa                    <  1:592205‑M2/92‑1 (MQ=255)
                                                                                 ccaGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCa                    >  1:1068856/1‑100 (MQ=255)
                                                                                  caGAGCAGGGTTGTCACTTTGCGTCCCGAACAGCGCTTCATAAGCCTGACCATCAACAACACCGCGATCACCTTTGACGTACACAGTGGATGCGGACCAt                   >  1:1033210/1‑100 (MQ=255)
                                                                                   agagCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATg                  >  2:1046721/1‑100 (MQ=255)
                                                                                   agagCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATg                  >  2:104201/1‑100 (MQ=255)
                                                                                      gcaGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCtt               >  1:820937‑M2/4‑100 (MQ=255)
                                                                                         gggTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCTATCAACCACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCg            >  1:40761/1‑100 (MQ=255)
                                                                                         gggTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGCATGCGGACCATGCTTTc             >  1:1412228/1‑99 (MQ=255)
                                                                                         gggTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCg            >  2:196245/1‑100 (MQ=255)
                                                                                           gTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGcc          <  2:1925364/100‑1 (MQ=255)
                                                                                             tGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCgg        >  2:1620957/1‑100 (MQ=255)
                                                                                              gTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGt       <  2:68069/100‑1 (MQ=255)
                                                                                               tCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGtt      <  1:801111/100‑1 (MQ=255)
                                                                                               tCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGtt      >  1:1487040/1‑100 (MQ=255)
                                                                                                cACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTc     >  2:1668816/1‑100 (MQ=255)
                                                                                                 aCTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTCg    <  1:1828543/100‑1 (MQ=255)
                                                                                                  cTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTCGt   >  2:1358585/1‑100 (MQ=255)
                                                                                                  cTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTCGt   <  2:1123186/100‑1 (MQ=255)
                                                                                                   tttGCGTCCAGAACAGCGCTTCATAAGCCTGCTCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTCGTa  <  2:196026/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCTCTTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTCGTA  >  NC_000913/3468290‑3468488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: