Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,362 C→A 15.8% intergenic (‑33/+38) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3620CA15.8% 329.6 / 45.8 125intergenic (‑33/+38)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base C (47/58);  new base A (14/6);  total (61/64)
Fisher's exact test for biased strand distribution p-value = 5.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01

Only 100 of 126 total aligned reads displayed.

GTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCA  >  NC_000913/3471264‑3471461
                                                                                                  |                                                                                                   
gTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCa                                                                                                    >  1:2247428/1‑100 (MQ=255)
gTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACGATAGTGCTCTCCCCTTCa                                                                                                    <  2:578998/100‑1 (MQ=255)
 tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                                   <  2:1131641/100‑1 (MQ=255)
 tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                                   >  1:2910123/1‑100 (MQ=255)
    aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                                <  2:1471780/100‑1 (MQ=255)
      gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                              <  1:454345/100‑1 (MQ=255)
        ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgaga                                                                                               >  1:125282/1‑97 (MQ=255)
         gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                           <  2:2449572/100‑1 (MQ=255)
          ccGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                                                          >  2:853928/1‑100 (MQ=255)
           cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                         <  2:2795188/100‑1 (MQ=25)
           cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                         <  2:2373576/100‑1 (MQ=25)
             aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                       <  2:2265659/100‑1 (MQ=255)
             aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                       <  2:1312596/100‑1 (MQ=255)
              tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATGTTCCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAct                                                                                        <  1:948795/98‑2 (MQ=25)
                gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                                    <  1:2233252/100‑1 (MQ=255)
                 tgccAACGTTAACGTGCGGTTTTGTACGTTCACTTTTTTCTTTAACCACGGCTATCATCCTTACTATAGTGCTCTCCCCTTCAGAAGTCAGCACgttac                                                                                    >  1:1859283/3‑95 (MQ=21)
                 tACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActatagt                                                                                   <  2:742729/100‑7 (MQ=25)
                 tACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACt                                                                                   >  2:435086/1‑100 (MQ=255)
                  aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACtt                                                                                  >  2:1643716/1‑100 (MQ=255)
                  aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACtt                                                                                  >  1:2287644/1‑100 (MQ=255)
                   ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACtt                                                                                  >  1:1714480/1‑99 (MQ=255)
                    cAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCATGAGAGAGCACGGGACTTTg                                                                                <  1:563987/100‑1 (MQ=255)
                    cAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTg                                                                                <  2:351645/100‑1 (MQ=255)
                     aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                               <  1:1113375/100‑1 (MQ=255)
                     aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                               <  2:1879856/100‑1 (MQ=255)
                     aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                               <  2:1417516/100‑1 (MQ=255)
                     aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                               <  1:334624/100‑1 (MQ=255)
                      aCGTTAACGTGTTGTTTTGTTCGTTCAAATTATAAATTAAAAAAAGATATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                              <  1:1759235/100‑1 (MQ=255)
                      aCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                              <  1:868680/100‑1 (MQ=255)
                      aCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCGATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                              <  1:1176569/100‑1 (MQ=255)
                       cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                               <  1:334714/98‑1 (MQ=255)
                       cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                             >  2:1322632/1‑100 (MQ=255)
                       cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                             >  2:406370/1‑100 (MQ=255)
                        gTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTCGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAt                                                                            <  1:2719822/100‑1 (MQ=255)
                        gTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAt                                                                            <  2:2829742/100‑1 (MQ=255)
                              gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAccc                                                                      >  2:2022137/1‑100 (MQ=255)
                              gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAccc                                                                      >  2:1465090/1‑100 (MQ=255)
                                 cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                                   <  2:2657413/100‑1 (MQ=255)
                                  ggTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAg                                                                  >  2:2106978/1‑100 (MQ=255)
                                   gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGATAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                 >  2:2175614/1‑100 (MQ=255)
                                   gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                 <  2:772803/100‑1 (MQ=255)
                                       tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                             >  1:164342/1‑100 (MQ=255)
                                       tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                             >  2:2529158/1‑100 (MQ=255)
                                            gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                        <  1:2855966/100‑1 (MQ=255)
                                            gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAAAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                        >  1:1582939/1‑100 (MQ=255)
                                             ttCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAcc                                                       <  1:1072030/100‑1 (MQ=255)
                                               cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCa                                                      >  2:2911086/1‑99 (MQ=255)
                                                 aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTTTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACg                                                    >  1:192655/1‑99 (MQ=255)
                                                 aaTTTTTCTTTAGACACGGCTATATTCCTTACGATAGTGATCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                                   <  1:2050635/100‑1 (MQ=255)
                                                  aTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg                                                  <  2:1394420/100‑1 (MQ=255)
                                                  aTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                                   <  1:1410142/99‑1 (MQ=255)
                                                    ttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGACAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                                <  1:1612479/100‑1 (MQ=255)
                                                      ttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAATCCTTAGGCTTATTTACCACGGGCTTc                                              <  2:1776576/100‑1 (MQ=255)
                                                        cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                            >  1:2283561/1‑100 (MQ=255)
                                                         tttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAt                                           >  2:729775/1‑100 (MQ=255)
                                                              acacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                      <  1:866035/100‑1 (MQ=255)
                                                              acacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                      <  2:570713/100‑1 (MQ=255)
                                                               cgcGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                     >  2:1092744/3‑100 (MQ=255)
                                                                acGGCTATATTCCTTACTATAGTGCTCTCCCCTTCTGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                       <  1:1847797/97‑1 (MQ=255)
                                                                    cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGATAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                                <  1:1303746/100‑1 (MQ=255)
                                                                    cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                                >  2:656320/1‑100 (MQ=255)
                                                                    cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                                >  2:467487/1‑100 (MQ=255)
                                                                      atatTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCa                              >  1:873598/1‑100 (MQ=255)
                                                                         ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                           >  2:639892/1‑100 (MQ=255)
                                                                          tCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGt                          >  1:162229/1‑100 (MQ=255)
                                                                          tCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGt                          <  1:424070/100‑1 (MQ=255)
                                                                          tCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGt                          >  1:59068/1‑100 (MQ=255)
                                                                            cTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGTACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTa                        >  2:1235134/1‑100 (MQ=255)
                                                                             ttACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTa                        <  2:1163484/99‑1 (MQ=255)
                                                                               aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                     <  1:1495846/100‑1 (MQ=255)
                                                                               aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                     <  1:36896/100‑1 (MQ=255)
                                                                                cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                    >  1:677832/1‑100 (MQ=255)
                                                                                cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                    <  2:2374812/100‑1 (MQ=255)
                                                                                 tcccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCgg                   <  1:247598/96‑1 (MQ=37)
                                                                                  cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                  >  2:2892252/4‑100 (MQ=38)
                                                                                  ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGTCTTCAATTACGGCCTGAGCAACGTTACTCGgc                  >  1:2488927/1‑100 (MQ=255)
                                                                                  ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                  <  1:2257877/100‑1 (MQ=255)
                                                                                  ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                  <  2:1035282/100‑1 (MQ=255)
                                                                                   ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                 >  1:488687/3‑100 (MQ=38)
                                                                                   ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                 >  2:2033924/3‑100 (MQ=38)
                                                                                   ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                 >  1:2227001/3‑100 (MQ=38)
                                                                                   ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                  >  1:60993/3‑99 (MQ=38)
                                                                                    cgTGCTCTCTCCTGAGGGGGAGAGCACGGGACTTTGGTATTACCCCTTAGGCTTGTGTACCACGGGCTTCAATGACGGCCTGAACAACGTTACTCGgcgc                >  1:1698401/2‑100 (MQ=255)
                                                                                    cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc                >  2:2285654/2‑100 (MQ=38)
                                                                                    cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc                >  2:497726/2‑100 (MQ=38)
                                                                                    cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc                >  2:473132/2‑100 (MQ=38)
                                                                                    cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc                >  1:1476327/2‑100 (MQ=38)
                                                                                     gTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                 >  1:1659630/1‑98 (MQ=39)
                                                                                     gTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt               >  1:2834228/1‑100 (MQ=39)
                                                                                       gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc             <  2:2284327/100‑1 (MQ=255)
                                                                                          ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc          <  1:2355849/100‑1 (MQ=255)
                                                                                            ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTcatcat        <  1:650437/100‑1 (MQ=255)
                                                                                            ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTcatcat        >  1:498194/1‑100 (MQ=255)
                                                                                            ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTcatcat        <  1:2897866/100‑1 (MQ=255)
                                                                                            ccccTTCAGGAGAGAGCACGGGACGTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc          <  2:2668909/98‑1 (MQ=255)
                                                                                              ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc      <  2:1979926/100‑1 (MQ=255)
                                                                                               cTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACt     <  2:492414/100‑1 (MQ=255)
                                                                                               cTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACt     <  2:795294/100‑1 (MQ=255)
                                                                                                ttCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc      <  1:2505000/98‑1 (MQ=255)
                                                                                                  cAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCa  <  2:1879026/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCA  >  NC_000913/3471264‑3471461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: