Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,810,324 G→A 15.5% intergenic (+20/+19) coaD → / ← mutM pantetheine‑phosphate adenylyltransferase/formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,810,3240GA15.5% 316.0 / 40.2 128intergenic (+20/+19)coaD/mutMpantetheine‑phosphate adenylyltransferase/formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base G (42/66);  new base A (3/17);  total (45/83)
Fisher's exact test for biased strand distribution p-value = 4.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 130 total aligned reads displayed.

AGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGAC  >  NC_000913/3810225‑3810422
                                                                                                   |                                                                                                  
agGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATg                                                                                                    <  2:1984268/100‑1 (MQ=255)
agGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATg                                                                                                    <  2:2558933/100‑1 (MQ=255)
 ggTGGCGCGCCATCAGGGCGATGTCCCCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGc                                                                                                   <  1:1378980/100‑1 (MQ=255)
 ggTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGc                                                                                                   >  2:1450917/1‑100 (MQ=255)
 ggTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGc                                                                                                   >  2:1372789/1‑100 (MQ=255)
  gTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGcc                                                                                                  <  1:2167702/100‑1 (MQ=255)
   tGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCa                                                                                                 <  1:452830/100‑1 (MQ=255)
        cgcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGCATACCATCCgg                                                                                            <  1:2080405/100‑1 (MQ=255)
        cgcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGCATACCATCCgg                                                                                            <  1:2115750/100‑1 (MQ=255)
        cgcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCAATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGTATGGCATACCATCCgg                                                                                            <  1:2047545/100‑1 (MQ=255)
         gcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGgc                                                                                           <  2:2477683/100‑1 (MQ=255)
         gcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGgc                                                                                           <  1:1537298/100‑1 (MQ=255)
         gcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGCATACCATCCGgc                                                                                           <  2:887080/100‑1 (MQ=255)
         gcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGCATACCATCCGgc                                                                                           <  2:1830662/100‑1 (MQ=255)
         gcCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGCATACCATCCGgc                                                                                           <  2:1660575/100‑1 (MQ=255)
          ccATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGgcg                                                                                          <  1:1014722/100‑1 (MQ=255)
          ccATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGCATACCATCCGgca                                                                                          <  2:1456759/100‑2 (MQ=255)
           cATCAGGGCGATGTCAGCCATTTCCTGCCGGATAATGTCCAGCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGCATACCATACGgca                                                                                          <  1:1001483/99‑2 (MQ=255)
           cATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGgcgc                                                                                         >  1:2886504/1‑100 (MQ=255)
           cATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGCATACCATCCGgcat                                                                                         <  1:1210455/100‑3 (MQ=255)
            aTCAGGGCGATGTCTCCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGCATACCATCCgg                                                                                            <  2:2412322/96‑1 (MQ=255)
            aTCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGgcgcg                                                                                        <  2:196218/100‑1 (MQ=255)
            aTCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGCATACCATCCGgca                                                                                          <  1:459600/98‑2 (MQ=255)
             tCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGgcgcgc                                                                                       <  2:2288571/100‑1 (MQ=255)
             tCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGgcgcgc                                                                                       >  1:112372/1‑100 (MQ=255)
               aGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCAt                                                                                     <  2:528203/100‑1 (MQ=255)
                gggCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCAt                                                                                     >  1:1462878/1‑99 (MQ=255)
                gggCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATg                                                                                    <  2:733045/100‑1 (MQ=255)
                gggCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATg                                                                                    >  2:1599679/1‑100 (MQ=255)
                  gCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGaa                                                                                  <  1:2058402/100‑1 (MQ=255)
                    gATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAtt                                                                                <  1:601640/100‑1 (MQ=255)
                    gATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAtt                                                                                <  1:2459506/100‑1 (MQ=255)
                    gATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAAt                                                                                 <  1:1308526/99‑1 (MQ=255)
                     aTGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGAGCTGATGGCGCAGTTAGCGTAGAGTTTCTGCCGGATGGTACGCCATCCGGCGCGCacgcacta                                                                               <  1:132714/100‑8 (MQ=255)
                       gTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACt                                                                             >  2:1682221/1‑100 (MQ=255)
                        tCACTCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACtt                                                                            <  2:2535878/100‑1 (MQ=255)
                         cACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTc                                                                           >  1:2648657/1‑100 (MQ=255)
                         cACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTc                                                                           >  1:2835643/1‑100 (MQ=255)
                           cccATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTg                                                                         <  1:1721736/100‑1 (MQ=255)
                             cATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATTGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTg                                                                         <  1:1448834/98‑1 (MQ=255)
                              aTTTCCTGCCGGAGAATGTCCATCAGGCGATGATGGCGATGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCTGGTTCGCCTGAATTACTTCTGGc                                                                       <  1:1373234/99‑1 (MQ=255)
                                ttCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGCTGGTCTGCCATCCGGCGCGCATGAATTACTTCTGGCACt                                                                    >  1:2355387/1‑100 (MQ=255)
                                ttCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTAAGCGTAGCGTTTATGCCGGATGGTACGCCATCCCGCGCGCATTGATCACTTCTGGCACt                                                                    >  1:678756/1‑100 (MQ=255)
                                   cTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTGTGGCACTGc                                                                  >  1:1916714/1‑99 (MQ=255)
                                   cTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGcc                                                                 >  1:354877/1‑100 (MQ=255)
                                   cTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGcc                                                                 >  2:2256772/1‑100 (MQ=255)
                                   cTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGcc                                                                 >  1:160132/1‑100 (MQ=255)
                                    tGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCg                                                                >  2:1357961/1‑100 (MQ=255)
                                     gCCGGTGAATGTCCATCAGGCGCTGATGGCTAAGTTAGCGTAGCGTTTATGCCGTATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGc                                                                       <  1:453424/92‑1 (MQ=255)
                                     gCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGa                                                               >  1:1868146/1‑100 (MQ=255)
                                     gCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGa                                                               >  2:1080924/1‑100 (MQ=255)
                                     gCCGGAGAATGTCCATCAAGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATACGGCGCGCATGAATTACTTCTGGCACTGCCGa                                                               >  1:909217/1‑100 (MQ=255)
                                      ccGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGAc                                                              <  1:1098413/100‑1 (MQ=255)
                                      ccGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGAc                                                              <  2:771151/100‑1 (MQ=255)
                                      ccGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGAc                                                              >  2:619998/1‑100 (MQ=255)
                                      ccGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGCATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGAc                                                              <  1:1611115/100‑1 (MQ=255)
                                       cGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACa                                                             >  2:2286426/1‑100 (MQ=255)
                                         gagaATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAAt                                                           <  2:2170793/100‑1 (MQ=255)
                                              tGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAAc                                                      <  2:1548073/100‑1 (MQ=255)
                                               gTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGTTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACg                                                     <  2:716092/100‑1 (MQ=255)
                                                tCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACg                                                     <  1:417093/99‑1 (MQ=255)
                                                tCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGt                                                    >  2:2336176/1‑100 (MQ=255)
                                                tCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGt                                                    <  1:2135867/100‑1 (MQ=255)
                                                tCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATACGGCGCGCATGAATTACTTATGGCACTGCCGACAATAAAACGt                                                    >  1:1670285/1‑100 (MQ=255)
                                                 ccATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGtt                                                   >  2:465662/1‑100 (MQ=255)
                                                  cATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTg                                                  <  2:1132848/100‑1 (MQ=255)
                                                  cATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTg                                                  <  1:2367952/100‑1 (MQ=255)
                                                  cATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTg                                                  <  2:522558/100‑1 (MQ=255)
                                                   aTCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGc                                                 <  1:2359346/100‑1 (MQ=255)
                                                   aTCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGc                                                 <  2:2801503/100‑1 (MQ=255)
                                                    tCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGcc                                                <  1:1622724/100‑1 (MQ=255)
                                                     cAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGccc                                               <  1:1724421/100‑1 (MQ=255)
                                                       ggCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGc                                             <  2:518083/100‑1 (MQ=255)
                                                         cgcTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTg                                           <  2:1403481/100‑1 (MQ=255)
                                                           cTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTgcg                                         <  1:1518918/100‑1 (MQ=255)
                                                             gATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCa                                       >  2:486519/1‑100 (MQ=255)
                                                             gATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCa                                       <  2:106811/100‑1 (MQ=255)
                                                             gATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCCACAATAAAACGTTGCCCGCTgcgc                                        >  1:956068/1‑99 (MQ=255)
                                                               tGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCGTGAATTACTTCTGGGACTGCCGACAATAAAACGTTGCCCGCTGCGCAt                                      >  1:645602/1‑99 (MQ=255)
                                                               tGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATg                                     <  2:1871892/100‑1 (MQ=255)
                                                                ggCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGt                                    >  2:1697850/1‑100 (MQ=255)
                                                                 gCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGtt                                   <  2:1838615/100‑1 (MQ=255)
                                                                     aGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGt                               <  1:2548675/100‑1 (MQ=255)
                                                                      gTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTc                              >  2:2483704/1‑100 (MQ=255)
                                                                         agcgtcgcgtTTATGCCGGATAGTCTGCCATCCGGCGCGCCTCAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGc                            <  1:966947/99‑1 (MQ=255)
                                                                         agcgtagcgtTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGcc                           <  1:1891240/100‑1 (MQ=255)
                                                                         agcgtagcgtTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGcc                           <  2:1071271/100‑1 (MQ=255)
                                                                           cgtagcgtTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTTCCGACAATAATACGTTGCCCGCTGCGCATGTTTAGTCGcctc                         <  2:1967612/100‑3 (MQ=255)
                                                                           cgtagcgtTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCcac                         <  2:2835662/100‑1 (MQ=255)
                                                                                  tttATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGc                  <  2:2821275/100‑1 (MQ=255)
                                                                                   ttATGCCGGATTGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCg                 <  2:964992/100‑1 (MQ=255)
                                                                                      cgCCGGATGGCATACCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTAc              >  1:1978329/2‑100 (MQ=255)
                                                                                      cgCCGGATGGCATACCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCAGCTGCGCATGTTTAGTCGCCACAATCGGCGTAc              >  1:1263402/2‑100 (MQ=255)
                                                                                        ccGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCg            >  2:233935/1‑100 (MQ=255)
                                                                                        ccGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCg            <  2:2463787/100‑1 (MQ=255)
                                                                                           gATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGcac         <  2:2648673/100‑1 (MQ=255)
                                                                                               gTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACAccc     <  1:675879/100‑1 (MQ=255)
                                                                                               gTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACAccc     <  2:2615805/100‑1 (MQ=255)
                                                                                               gTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACAccc     >  2:901492/1‑100 (MQ=255)
                                                                                                  tGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGac  >  2:404146/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
AGGTGGCGCGCCATCAGGGCGATGTCACCCATTTCCTGCCGGAGAATGTCCATCAGGCGCTGATGGCGAAGTTAGCGTAGCGTTTATGCCGGATGGTATGCCATCCGGCGCGCATGAATTACTTCTGGCACTGCCGACAATAAAACGTTGCCCGCTGCGCATGTTTAGTCGCCACAATCGGCGTACCGCACACCCGAC  >  NC_000913/3810225‑3810422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: