Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,130,008 T→C 15.0% I58I (ATT→ATC metL → Bifunctional aspartokinase/homoserine dehydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,130,0080TC15.0% 272.7 / 40.7 121I58I (ATT→ATCmetLBifunctional aspartokinase/homoserine dehydrogenase 2
Reads supporting (aligned to +/- strand):  ref base T (52/49);  new base C (10/8);  total (63/58)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-01

Only 100 of 124 total aligned reads displayed.

GAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCC  >  NC_000913/4129909‑4130107
                                                                                                    |                                                                                                    
     gtTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTg                                                                                                 >  1:1941900/1‑100 (MQ=255)
      ttATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTgg                                                                                                <  2:2291121/100‑1 (MQ=255)
      ttATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTgg                                                                                                <  1:1180579/100‑1 (MQ=255)
      ttATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTgg                                                                                                <  2:2452536/100‑1 (MQ=255)
        aTTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGtt                                                                                              <  1:642856/100‑1 (MQ=255)
        aTTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTAATCAACTGGtt                                                                                              <  2:1639963/100‑1 (MQ=255)
         tttGCGTGTCGCGGGCATTATGGCGGAGTA‑‑CTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTAATCAACTGGttagt                                                                                           <  1:1219948/100‑4 (MQ=255)
         tgtGCGGGGCGCGGGCATTATGGCGGAGTGCTCTCAGCCTGACGATATGAGGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTg                                                                                             <  1:2296817/98‑1 (MQ=255)
          ttGCGTGTCGCGGGCAGTATGGCGTAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTAATCAACTGGttag                                                                                            <  1:2560776/100‑3 (MQ=255)
           tGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTAATCAACTGGttagt                                                                                           <  1:2075802/100‑4 (MQ=255)
           tGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTAATCAACTGGttagt                                                                                           <  1:1047065/100‑4 (MQ=255)
            gCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTCACCAGTTGATTACCTGGTTGaaa                                                                                          >  2:2728486/1‑100 (MQ=255)
             cGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAAc                                                                                         <  1:1431625/100‑1 (MQ=255)
             cGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAAc                                                                                         >  2:345389/1‑100 (MQ=255)
             cGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTAATCAACTGGttagtgg                                                                                         <  1:1883893/100‑6 (MQ=255)
             cGTGCCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATTATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTgg                                                                                                <  1:697038/93‑1 (MQ=255)
              gtgtCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACt                                                                                        <  1:518765/100‑1 (MQ=255)
               tgtCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTa                                                                                       >  2:1660973/1‑100 (MQ=255)
                gtCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTaa                                                                                      >  1:1180068/1‑100 (MQ=255)
                  cgcgGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGc                                                                                    >  2:1730672/1‑100 (MQ=255)
                   gcgGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACTAGTTGATTAACTGGTTGAAACTAAGcc                                                                                   >  2:769979/1‑100 (MQ=255)
                    cgGGCATTATGGCGGAGTACTCGTAGCCTGACGATATGAGGGTGGTTTCCGCCTCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGcc                                                                                   <  2:2079993/99‑1 (MQ=255)
                        cATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGAcc                                                                              >  2:1331355/1‑100 (MQ=255)
                           tttGGCGGGGTACTTTTCGGCTGTCGGTATGATGGTGGTTTTCGGCGGCCGTTAGACCCCTAACCAGTTGATTTAATGGTTGAAACTAAGGCCAACCGAt                                                                          >  1:2489336/3‑100 (MQ=255)
                            aTGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATc                                                                         >  2:268449/1‑100 (MQ=255)
                               gCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGtc                                                                      >  1:1056505/1‑100 (MQ=255)
                                cGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGtct                                                                     >  2:943141/1‑100 (MQ=255)
                                cGGAGTACTCTCAGCCTCACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGtct                                                                     >  1:1097265/1‑100 (MQ=255)
                                   aGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGtctctc                                                                  <  2:1205743/100‑1 (MQ=255)
                                     tACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTg                                                                <  2:1414794/100‑1 (MQ=255)
                                       ctctCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCAGATCggaagagcac                                                               >  2:867910/1‑91 (MQ=255)
                                         ctcAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGCCCGATCGTCTCTCTGCGCa                                                            >  1:2476346/1‑100 (MQ=255)
                                         ctcAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCa                                                            <  1:2807761/100‑1 (MQ=255)
                                           cAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATc                                                          <  1:1984400/100‑1 (MQ=255)
                                           cAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATc                                                          >  2:48998/1‑100 (MQ=255)
                                            aGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCa                                                         <  2:1596924/100‑1 (MQ=255)
                                            aGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCa                                                         <  1:91409/100‑1 (MQ=255)
                                             gCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTCACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTCCGCACCAg                                                        >  1:2196905/1‑100 (MQ=255)
                                             gCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAATCTAAGCCAGACCGATCGTCTCTCTGCGCATCAg                                                        <  1:1015051/100‑1 (MQ=255)
                                               cTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGt                                                      <  1:302630/100‑1 (MQ=255)
                                               cTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGt                                                      <  1:83995/100‑1 (MQ=255)
                                               cTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGt                                                      >  2:1190707/1‑100 (MQ=255)
                                                  aCGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTca                                                   <  1:1581691/100‑1 (MQ=255)
                                                    gATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTcaac                                                 >  2:2268918/1‑100 (MQ=255)
                                                        tgatgGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAAc                                             <  2:2451/100‑1 (MQ=255)
                                                          atgGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGc                                           <  1:2316757/100‑1 (MQ=255)
                                                            ggtggtTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTg                                         <  2:462539/100‑1 (MQ=255)
                                                            ggtggtTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTg                                         >  2:2391320/1‑100 (MQ=255)
                                                             gtggtTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACACACGCTGc                                        >  1:2645273/1‑100 (MQ=255)
                                                             gtggtTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGc                                        <  2:2628058/100‑1 (MQ=255)
                                                             gtggtTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGc                                        >  2:107281/1‑100 (MQ=255)
                                                                gtTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTc                                     >  1:649381/1‑100 (MQ=255)
                                                                gtTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTc                                     >  2:1945973/1‑100 (MQ=255)
                                                                 tttCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCg                                    <  2:829260/100‑1 (MQ=255)
                                                                  ttCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGc                                   <  1:2407401/100‑1 (MQ=255)
                                                                  ttCCGCCGCCGGTAGCACCACTAACCAGCCGATTAACTGGTTGAAACTACGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGAGTc                                     >  1:2783744/1‑98 (MQ=255)
                                                                   tCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGc                                   <  2:858785/99‑1 (MQ=255)
                                                                    ccgccgccgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTa                                 >  2:1460990/1‑100 (MQ=255)
                                                                    ccgccgccgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTa                                 >  1:1349280/1‑100 (MQ=255)
                                                                    ccgccgccgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTa                                 >  2:782046/1‑100 (MQ=255)
                                                                    ccgccgccgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTa                                 >  2:2721689/1‑100 (MQ=255)
                                                                     cgccgccgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTAt                                >  2:1693720/1‑100 (MQ=255)
                                                                     cgccgccgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTAt                                >  1:933411/1‑100 (MQ=255)
                                                                      gccgccgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATc                               <  1:1845910/100‑1 (MQ=255)
                                                                      gccgccgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATc                               >  1:1486366/1‑100 (MQ=255)
                                                                        cgccgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAg                             <  2:1426971/100‑1 (MQ=255)
                                                                          gccgGTAGCACCACTAACAAAAAGATGAACTGGTTGAAACTAAGCCAGACCGATCGACTCTATGCGCAACAAGAACAAAAAACGCTGCGTCGCTAACAGt                            >  1:533094/1‑100 (MQ=255)
                                                                           ccgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTg                           <  2:2543443/100‑1 (MQ=255)
                                                                           ccgGTAGCACCACTAACCAGTTGATGAACTGGTTGAAACTACGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTg                           <  2:2543947/100‑1 (MQ=255)
                                                                            cgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGc                          <  2:2476666/100‑1 (MQ=255)
                                                                            cgGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGc                          >  2:1031392/1‑100 (MQ=255)
                                                                             ggTAGCACCACTAACCAGTTGGTTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGATTCAACAAACGCTGCGTCGCTATCAGTGCg                         >  1:2877187/1‑100 (MQ=255)
                                                                             ggTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCg                         >  2:2094575/1‑100 (MQ=255)
                                                                             ggTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGGCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCg                         >  2:860130/1‑100 (MQ=255)
                                                                               tAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGAt                       >  2:467804/1‑100 (MQ=255)
                                                                                   accacTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGa                   >  1:104561/1‑100 (MQ=255)
                                                                                    ccacTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGAt                  >  1:2167963/1‑100 (MQ=255)
                                                                                     tttcaaCCAGTTAATCAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGAtt                 >  2:2748294/5‑100 (MQ=255)
                                                                                     tttcaaCCAGTTAATCAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTCCAACAAACGCTGCGTCGCTATCAGTGCGATCTGAtt                 >  1:1723711/5‑100 (MQ=255)
                                                                                      ttcaaCCAGTTAATCAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTa                >  2:2103472/4‑100 (MQ=255)
                                                                                      ttcaaCCAGTTAATCAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTa                >  2:1363201/4‑100 (MQ=255)
                                                                                        tAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGc              <  2:2211135/100‑1 (MQ=255)
                                                                                        tAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGc              <  2:2772095/100‑1 (MQ=255)
                                                                                        caaCCAGTTAATCAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGTTCTGATTAGc              >  1:1919834/2‑100 (MQ=255)
                                                                                        caaCCAGTTAATCAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGc              >  1:1457016/2‑100 (MQ=255)
                                                                                         aaCCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCg             >  2:1609263/1‑100 (MQ=255)
                                                                                         aaCCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCg             <  2:1774052/100‑1 (MQ=255)
                                                                                         aaCCAGTTAATCAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACACACGCTGCGTCGCTATCAGTGCGATCTGATTAGCg             >  1:2196735/1‑100 (MQ=255)
                                                                                           ncAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGt           >  2:1777175/2‑100 (MQ=255)
                                                                                           ccAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTTTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGc              <  1:2383002/97‑1 (MQ=255)
                                                                                            cAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTc          <  2:2373929/100‑1 (MQ=255)
                                                                                            cAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTc          >  2:673326/1‑100 (MQ=255)
                                                                                            cAGTTGATTAACTGGTGGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAGCGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTc          >  1:335144/1‑100 (MQ=255)
                                                                                             aGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCt         >  2:2760840/1‑100 (MQ=255)
                                                                                             aGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCt         >  2:2603708/1‑100 (MQ=255)
                                                                                               ttGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGc       <  2:1983497/100‑1 (MQ=255)
                                                                                                tGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCt      <  2:1825782/100‑1 (MQ=255)
                                                                                                 gATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTa     <  1:1445934/100‑1 (MQ=255)
                                                                                                  aTTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTAc    <  1:1607458/100‑1 (MQ=255)
                                                                                                   ttAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTAcc   >  2:1906308/1‑100 (MQ=255)
                                                                                                    |                                                                                                    
GAAGTGTTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCACTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGCTATCAGTGCGATCTGATTAGCGGTCTGCTACCC  >  NC_000913/4129909‑4130107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: