Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,214,649 A→T 81.5% I124F (ATC→TTC)  metA → homoserine O‑transsuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,214,6490AT81.5% 264.7 / 47.1 112I124F (ATC→TTC) metAhomoserine O‑transsuccinylase
Reads supporting (aligned to +/- strand):  ref base A (12/9);  new base T (48/43);  total (60/52)
Fisher's exact test for biased strand distribution p-value = 8.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

AACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATT  >  NC_000913/4214550‑4214744
                                                                                                   |                                                                                               
aaCTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGa                                                                                                 >  1:1972192/1‑100 (MQ=255)
  cTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTc                                                                                               <  2:878312/100‑1 (MQ=255)
  cTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTc                                                                                               >  1:1967168/1‑100 (MQ=255)
    ttGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTc                                                                                               >  1:141278/1‑98 (MQ=255)
     tGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCCGTTCaaa                                                                                            <  2:1524638/100‑1 (MQ=255)
     tGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCaaa                                                                                            >  2:1131818/1‑100 (MQ=255)
     tGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCaaa                                                                                            >  1:2870624/1‑100 (MQ=255)
      gAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCaaan                                                                                           <  1:388333/100‑2 (MQ=255)
       aaGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACa                                                                                          <  2:1462015/100‑1 (MQ=255)
        aGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAAc                                                                                           <  2:1071332/98‑1 (MQ=255)
        aGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAg                                                                                         >  2:2328142/1‑100 (MQ=255)
         gATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAgg                                                                                        <  1:230409/100‑1 (MQ=255)
         gATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAgg                                                                                        <  2:649383/100‑1 (MQ=255)
         gATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAgg                                                                                        <  1:1699852/100‑1 (MQ=255)
            atTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGc                                                                                     >  1:1670326/1‑100 (MQ=255)
             ttCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCt                                                                                    >  2:2593860/1‑100 (MQ=255)
             ttCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCt                                                                                    >  1:2014915/1‑100 (MQ=255)
             ttCAGGATCAGAACTTTGACGGGTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGCGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGc                                                                                     >  1:2791741/1‑99 (MQ=255)
              tCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTg                                                                                   >  1:272391/1‑100 (MQ=255)
              tCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTg                                                                                   >  1:1461528/1‑100 (MQ=255)
              tCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCATGGTGGAGTTTAATGATGTCGCTTCCTGGCCGCAGTTCAAACAGGTGCTg                                                                                   >  1:121767/1‑100 (MQ=255)
               cAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTgg                                                                                  >  2:2276350/1‑100 (MQ=255)
                  ngtCAGAACTTTGCCGGTTTGATTGTAACTGATGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGt                                                                               >  2:1968911/3‑100 (MQ=255)
                  gATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGt                                                                               >  1:559535/1‑100 (MQ=255)
                  gATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGACCGCAGTTCAAACAGGTGCTGGAg                                                                                >  2:2629221/1‑99 (MQ=255)
                   aTCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTg                                                                              >  2:1054757/1‑100 (MQ=255)
                    tCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTgg                                                                             <  1:985086/100‑1 (MQ=255)
                     cAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTTGCCGCAGTTCAAACAGGTTCTGGAGTGGt                                                                            >  1:638475/1‑100 (MQ=255)
                      aGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTc                                                                           >  2:1407369/1‑100 (MQ=255)
                      aGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTc                                                                           >  2:2523958/1‑100 (MQ=255)
                      aGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTc                                                                           <  2:2041349/100‑1 (MQ=255)
                          cTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGaaa                                                                       >  2:398838/1‑100 (MQ=255)
                          cTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGaaa                                                                       >  2:2702512/1‑100 (MQ=255)
                          cTTTGACGGTTTGATTGTAACTGGTGCGCCACTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGaaa                                                                       <  1:2402037/100‑1 (MQ=255)
                           tttGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAg                                                                      <  2:1748730/100‑1 (MQ=255)
                              gACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATc                                                                   <  1:772044/100‑1 (MQ=255)
                              gACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATc                                                                   <  2:263080/100‑1 (MQ=255)
                               aCGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCa                                                                  <  2:553020/100‑1 (MQ=255)
                                 ggTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCa                                                                  <  1:2837604/98‑1 (MQ=255)
                                 ggTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACg                                                                <  1:2276335/100‑1 (MQ=255)
                                   tttGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTc                                                              <  1:1730408/100‑1 (MQ=255)
                                     gtaTTGGAACTGGGGCGCCGCTGGGCCTGGTGGGGTTTAATCATGTCGCTTACTGTCCGCAGTTCAGACAGGTGCTCGAGGGGTCGCAAGATCCCGTCa                                                             >  1:2769439/3‑99 (MQ=255)
                                      gATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCagc                                                           <  2:1967179/100‑3 (MQ=255)
                                      gATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCAcc                                                           <  2:1902670/100‑1 (MQ=255)
                                        ttGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCAcccc                                                         <  2:1958175/100‑3 (MQ=255)
                                        ttGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTc                                                         >  2:565396/1‑100 (MQ=255)
                                        ttGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTc                                                         >  2:712317/1‑100 (MQ=255)
                                        ttGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTc                                                         >  1:2537200/1‑100 (MQ=255)
                                        ttGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTc                                                         <  2:2404045/100‑1 (MQ=255)
                                           tAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCCGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGAc                                                      >  1:200768/1‑100 (MQ=255)
                                           tAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGAc                                                      >  1:2867654/1‑100 (MQ=255)
                                           tAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGAc                                                      <  2:434096/100‑1 (MQ=255)
                                            aaCTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACg                                                     >  1:985740/1‑100 (MQ=255)
                                            aaCTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTAGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACg                                                     >  1:1297635/1‑100 (MQ=255)
                                             aCTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGc                                                    <  2:1705903/100‑1 (MQ=255)
                                                 gTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTGGCTTACTGGCCGCAGTTGAAACAGGTGCTGGAGTGGTCGCAAGATCACGTCACCTCGACGCTGt                                                 >  1:1319574/1‑99 (MQ=255)
                                                 gTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGtt                                                <  2:2816140/100‑1 (MQ=255)
                                                  tGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACCGGTGCTGGAGTGGTCGAAAGATCACGCCACCTCGACGCTGttt                                               >  1:622952/1‑100 (MQ=255)
                                                   gcgcCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTg                                              <  1:2499458/100‑1 (MQ=255)
                                                   gcgcCGCTGGGCCTGGTGGAGATTAATGATGTCGCTTGCTGGCCGCAGTTCACACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGCCGCTGTTTg                                              >  1:1855750/1‑100 (MQ=255)
                                                      ccgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTct                                           >  1:1632105/1‑100 (MQ=255)
                                                          tGGGCCTGATGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctgctg                                       <  1:121614/100‑1 (MQ=255)
                                                           gggCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctgctgn                                      <  1:1669833/100‑2 (MQ=255)
                                                           gggCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTgg                                      <  2:2881478/100‑1 (MQ=255)
                                                            ggCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCATGTCACCTCGACGCCGTCTGTCTGCTgg                                      >  1:2198008/1‑99 (MQ=255)
                                                            ggCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTggg                                     <  1:315982/100‑1 (MQ=255)
                                                             gCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGc                                    <  2:1567843/100‑1 (MQ=255)
                                                             gCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGc                                    <  1:2883401/100‑1 (MQ=255)
                                                              ccTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCg                                   <  2:1172730/100‑1 (MQ=255)
                                                              ccTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCg                                   <  1:1216993/100‑1 (MQ=255)
                                                              ccTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCg                                   <  1:155674/100‑1 (MQ=255)
                                                               cTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCgg                                  <  2:404483/100‑1 (MQ=255)
                                                               cTGGGGGAGTTTAATGATGTCGCTTACTGGCCGCGCTTCAAACAGGTGCTGGAGAGGTCGTAAGATCACGTCTCGTCGACGCTGTTTGTctgctg                                       >  1:411952/1‑95 (MQ=255)
                                                                tggtggAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGt                                 >  1:2123110/1‑100 (MQ=255)
                                                                  tccgatcttAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTAc                               <  1:1316034/93‑1 (MQ=255)
                                                                  gtggAGTTTAATGATGCCGCTTACTGGCCGCAGTTCAAACAGCTGCTGGAGTGGACCAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGCGCCGTAc                               >  1:1295095/1‑100 (MQ=255)
                                                                  gaggAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTAc                               >  2:1967835/3‑100 (MQ=255)
                                                                   tggAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACa                              <  1:2421496/100‑1 (MQ=255)
                                                                    ggAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAg                             >  2:537651/1‑100 (MQ=255)
                                                                     gAGTTTAATGATGTCGTTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAgg                            <  1:1700515/100‑1 (MQ=255)
                                                                      aGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGATTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAgg                            <  1:402336/99‑1 (MQ=255)
                                                                       gTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGcc                          >  1:2541631/1‑100 (MQ=255)
                                                                         ttAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCcgc                        >  2:1207128/1‑100 (MQ=255)
                                                                          tAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAgatcgga                       >  2:1316039/1‑94 (MQ=255)
                                                                          tAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCcgcg                       >  1:509301/1‑100 (MQ=255)
                                                                           aaTGATGTCGCTTACTGGCCGCAGTTCAAGCAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCgcgc                      <  1:803666/100‑1 (MQ=255)
                                                                            atgatgTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCt                     <  1:2333555/100‑1 (MQ=255)
                                                                                tgTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAat                 >  1:53220/1‑100 (MQ=255)
                                                                                 gTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAata                >  2:1709636/1‑100 (MQ=255)
                                                                                  tCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACCGGCCGCGCTCAatat               >  1:391086/1‑100 (MQ=255)
                                                                                   cGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAatat               >  1:234760/1‑99 (MQ=255)
                                                                                    gCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATcc             <  1:18361/100‑1 (MQ=255)
                                                                                     cTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCct            >  1:148348/1‑100 (MQ=255)
                                                                                         cTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTAc        >  2:1933609/1‑100 (MQ=255)
                                                                                         cTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCCGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTAc        >  1:1870030/1‑100 (MQ=255)
                                                                                          tGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACg       >  2:1993537/1‑100 (MQ=255)
                                                                                           ggCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACgg      >  2:1421059/1‑100 (MQ=255)
                                                                                           ggCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCGCAATATCCTCTACgg      >  2:2874398/1‑100 (MQ=255)
                                                                                            gCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGc     <  2:2818058/100‑1 (MQ=255)
                                                                                               gCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCAtt  <  2:509304/100‑1 (MQ=255)
                                                                                                   |                                                                                               
AACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATT  >  NC_000913/4214550‑4214744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: