Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,243,370 C→T 81.1% L49L (CTG→CTA malG ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,243,3700CT81.1% 205.1 / 46.9 106L49L (CTG→CTAmalGmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (10/10);  new base T (36/49);  total (46/60)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

Only 100 of 109 total aligned reads displayed.

CAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGA  >  NC_000913/4243271‑4243469
                                                                                                   |                                                                                                   
cagTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATc                                                                                                     >  1:2420862/1‑100 (MQ=255)
  gTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAg                                                                                                   <  1:1715947/100‑1 (MQ=255)
  gTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAg                                                                                                   >  1:619652/1‑100 (MQ=255)
    aCCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGc                                                                                                 <  2:564899/100‑1 (MQ=255)
    aCCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGc                                                                                                 <  1:1062884/100‑1 (MQ=255)
    aCCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAAACTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAgg                                                                                                  >  1:709737/1‑99 (MQ=255)
     cctggAATGGCGGTGGCGTCATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATTCCAGGAGATTTGCTCCGGGATTAGGc                                                                                                 <  1:1767010/96‑1 (MQ=255)
     ccGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCt                                                                                                >  1:1913858/1‑100 (MQ=255)
     ccGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCt                                                                                                >  1:216228/1‑100 (MQ=255)
      cGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTg                                                                                               <  1:2569699/100‑1 (MQ=255)
       gggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGc                                                                                              >  2:838662/1‑100 (MQ=255)
        ggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGcc                                                                                             <  2:2110116/100‑1 (MQ=255)
        ggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGcc                                                                                             >  2:1606504/1‑100 (MQ=255)
        ggAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGcc                                                                                             <  2:383625/100‑1 (MQ=255)
         gAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCg                                                                                            >  2:2121913/1‑100 (MQ=255)
         gAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCg                                                                                            <  2:1225895/100‑1 (MQ=255)
          aaTGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCgg                                                                                           <  2:2149758/100‑1 (MQ=255)
              gCGGTGGCGTAATGTGACCATCAGCCTGTTGAACGCTAAAACATAACGCCAGTTTCCAGTGAACCCAGGAAAACTGCTCCGGGATTAGGCTGCCGTTCGc                                                                                       >  1:1222739/1‑100 (MQ=255)
                  ggaaactttGCGACCATCAGCCTGTGCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAg                                                                                   <  1:380515/92‑1 (MQ=255)
                    gCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGtt                                                                                 <  2:1040884/100‑1 (MQ=255)
                      gTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTc                                                                               <  2:1886203/100‑1 (MQ=255)
                       tAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTcc                                                                              >  2:168797/1‑100 (MQ=255)
                        aaTGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTccc                                                                             <  1:1818468/100‑1 (MQ=255)
                        aaTGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTccc                                                                             <  1:1102349/100‑1 (MQ=255)
                        aaTGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTccc                                                                             <  2:623549/100‑1 (MQ=255)
                          tGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTg                                                                           <  1:2387408/100‑1 (MQ=255)
                          tGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTg                                                                           >  2:1274809/1‑100 (MQ=255)
                            cGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTTATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGAc                                                                         <  2:713579/100‑1 (MQ=255)
                            cGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGAc                                                                         >  1:2478128/1‑100 (MQ=255)
                               ccATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCa                                                                      >  2:1948403/1‑100 (MQ=255)
                                cATCAGCCTGTTCAACGCTAAAGCCTAGCGCCAGTTTCCAGTGAGCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTGCCCCTGACGCAg                                                                     <  1:2356629/100‑1 (MQ=255)
                                cATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAg                                                                     <  1:1416042/100‑1 (MQ=255)
                                cATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAg                                                                     >  1:1344972/1‑100 (MQ=255)
                                  tCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCg                                                                   >  2:2338639/1‑100 (MQ=255)
                                    aGCCTGTTCAACGCTAAAACCTGACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgag                                                                 >  1:1691513/1‑100 (MQ=255)
                                    aGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgag                                                                 <  1:1031707/100‑1 (MQ=255)
                                     gCCTGTTCAACGCTACAACCTCACGCCAGTCTCCAGTGATCCCCGGAGATTTGCTCCGGGAATAGGCTGCCGGTCGCAAAGTTTCCCt                                                                            >  1:302940/1‑88 (MQ=255)
                                     gCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgaga                                                                >  2:2661770/1‑100 (MQ=255)
                                     gCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgaga                                                                <  2:2781882/100‑1 (MQ=255)
                                      ccTGTTCAACGCTAAGACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGAt                                                               <  2:2879206/100‑1 (MQ=255)
                                            cttgttaaaaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgac                                                         <  1:1902581/93‑1 (MQ=255)
                                            cAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCCAAGTTTCCCTGACGCAGCGAGATAGcgac                                                         >  1:1531571/1‑100 (MQ=255)
                                            cAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgac                                                         >  1:2271418/1‑100 (MQ=255)
                                            cAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTACCTGACGAAGGGAGCTAGcgac                                                         >  2:537062/1‑100 (MQ=255)
                                             aaCGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgacg                                                        >  1:292200/1‑100 (MQ=255)
                                             aaCGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgacg                                                        <  1:2302475/100‑1 (MQ=255)
                                             aaCGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCCCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgac                                                         >  1:331858/1‑99 (MQ=255)
                                              aCGCTAAAGCCGTACGCTATTTTCCAGTGATCGCAGCAGATTTGCTCCGGGATTAGGCTGCCGGTCGCACATTTTCCCGTATGCAGCGAGATAGcg                                                           <  1:670156/96‑1 (MQ=255)
                                                acTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGAcc                                                     <  2:75158/99‑1 (MQ=255)
                                                  tAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCAt                                                   <  2:223442/100‑1 (MQ=255)
                                                   aaaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATc                                                  <  2:2438465/100‑1 (MQ=255)
                                                   aaaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATc                                                  <  2:2437754/100‑1 (MQ=255)
                                                    aaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATca                                                 <  2:1062264/100‑1 (MQ=255)
                                                    aaaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATca                                                 <  1:629137/100‑1 (MQ=255)
                                                     aaCCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATcag                                                >  2:216278/1‑100 (MQ=255)
                                                         tAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCatcagc                                            >  1:824153/1‑100 (MQ=255)
                                                         tAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCagcagc                                            >  2:1836668/1‑100 (MQ=255)
                                                           aCGCCAGTTTCCAGTGATCGCAGGAGATTTGGTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCgn                                          <  1:2366046/100‑2 (MQ=255)
                                                            cGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCggg                                         >  2:2596880/1‑100 (MQ=255)
                                                             gCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGa                                        <  1:168054/100‑1 (MQ=255)
                                                              ccAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGaa                                       <  2:2111254/100‑1 (MQ=255)
                                                              ccAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGaa                                       >  1:1645427/1‑100 (MQ=255)
                                                               cAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAAc                                      <  2:823195/100‑1 (MQ=255)
                                                                aGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACa                                     <  1:1130963/100‑1 (MQ=255)
                                                                aGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACa                                     <  2:1558822/100‑1 (MQ=255)
                                                                aGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACa                                     <  2:2681556/100‑1 (MQ=255)
                                                                 gTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACAt                                    >  1:356551/1‑100 (MQ=255)
                                                                  tttCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATa                                   >  2:2361490/1‑100 (MQ=255)
                                                                    tCCAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAAt                                 <  2:2370609/100‑1 (MQ=255)
                                                                     ccAGTGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACaca                                   >  1:2508498/1‑95 (MQ=255)
                                                                      cAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCg                               >  1:2255732/1‑100 (MQ=255)
                                                                         tGATCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTg                            <  2:790596/100‑1 (MQ=255)
                                                                         tGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTg                            <  1:1937148/100‑1 (MQ=255)
                                                                           aTCCCAGGAGATTTGCTGCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTg                            >  1:976735/1‑98 (MQ=255)
                                                                           aTCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCg                          <  1:2661754/100‑1 (MQ=255)
                                                                           aTCCCAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCg                          <  1:1606498/100‑1 (MQ=255)
                                                                             cccGGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGTAGCGGGAACATAATCGCTGCGAt                        >  1:695288/1‑100 (MQ=255)
                                                                             cccAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGAt                        >  2:1623786/1‑100 (MQ=255)
                                                                              ccAGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATa                       <  1:958214/100‑1 (MQ=255)
                                                                              ccAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATa                       >  2:32624/1‑100 (MQ=255)
                                                                                aGGAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa                     <  2:1140419/100‑1 (MQ=255)
                                                                                 ggAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaaa                    <  2:2004675/100‑1 (MQ=255)
                                                                                 ggAGATTTGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaaa                    >  1:2080632/1‑100 (MQ=255)
                                                                                 ggAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaaa                    >  2:124983/1‑100 (MQ=255)
                                                                                      tttGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtag               <  2:1812582/100‑1 (MQ=255)
                                                                                      tttGCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtag               <  1:54116/100‑1 (MQ=255)
                                                                                       tgtcTCCGGGATTATGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCTAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagta                <  1:1004580/95‑1 (MQ=255)
                                                                                        tGCTCCGGGAGTAGGCTGCCGGTCGCAAAGTTTCCCGGACGCAGCGAGATAGAGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtag               <  1:2913646/98‑1 (MQ=255)
                                                                                         gCTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcag            <  2:2648743/100‑1 (MQ=255)
                                                                                         gCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAtgtagcag            <  2:1522877/100‑1 (MQ=255)
                                                                                          ctctGGGATGAGGCTGCCGCGCGCAAAGTTGCACTGACTCAGCGAGAGAGCGACAACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcag            <  1:2466876/99‑1 (MQ=255)
                                                                                          cTCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagt           >  2:846763/1‑100 (MQ=255)
                                                                                          cTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagt           >  1:2285841/1‑100 (MQ=255)
                                                                                           tCCGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagta          >  2:1930739/1‑100 (MQ=255)
                                                                                             cGGGATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagtagc        >  1:382880/1‑100 (MQ=255)
                                                                                              gggATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAgtagcagtagca       <  2:2900865/100‑1 (MQ=255)
                                                                                                gATTAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAgg     >  2:335089/1‑100 (MQ=255)
                                                                                                  tCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTg   >  1:1508721/1‑100 (MQ=255)
                                                                                                   cAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGa  <  2:499346/100‑1 (MQ=255)
                                                                                                   cAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGa  >  2:2781938/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGA  >  NC_000913/4243271‑4243469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: