Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,295,744 G→A 100% A422T (GCT→ACT)  gltP → glutamate/aspartate:proton symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,7440GA92.8% 372.6 / 13.1 127A422T (GCT→ACT) gltPglutamate/aspartate:proton symporter
Reads supporting (aligned to +/- strand):  ref base G (5/4);  new base A (61/56);  total (66/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 127 total aligned reads displayed.

CGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTG  >  NC_000913/4295647‑4295839
                                                                                                 |                                                                                               
cGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACt                                                                                               <  1:1877350/100‑1 (MQ=255)
cGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACt                                                                                               <  2:1043502/100‑1 (MQ=255)
 gACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACtt                                                                                              >  1:296238/1‑100 (MQ=255)
 gACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACtt                                                                                              <  1:982948/100‑1 (MQ=255)
  aCATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTa                                                                                             <  2:321102/100‑1 (MQ=255)
      ggCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAg                                                                                         <  1:2282297/100‑1 (MQ=255)
        cgcgTACTGCTCTGGACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTAtn                                                                                           <  1:621963/96‑2 (MQ=255)
        cgcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCg                                                                                       <  2:559913/100‑1 (MQ=255)
          cgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTg                                                                                     <  1:1135573/100‑1 (MQ=255)
           gTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGa                                                                                    >  1:552265/1‑100 (MQ=255)
            tACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGaa                                                                                   >  2:272170/1‑100 (MQ=255)
              cTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGt                                                                                 >  2:1662513/1‑100 (MQ=255)
              cTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGt                                                                                 <  2:2276232/100‑1 (MQ=255)
              cTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTACTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGt                                                                                 >  1:2018223/1‑100 (MQ=255)
               tGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTg                                                                                <  1:2576673/100‑1 (MQ=255)
                gcgcTGAACGGGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGc                                                                               >  1:2834870/1‑100 (MQ=255)
                  gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTg                                                                             <  2:2034920/100‑1 (MQ=255)
                  gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTg                                                                             >  2:406527/1‑100 (MQ=255)
                  gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTg                                                                             >  2:943661/1‑100 (MQ=255)
                   cTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTgg                                                                            >  1:1211304/1‑100 (MQ=255)
                     gAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTCCTGGGc                                                                          >  1:2777916/1‑100 (MQ=255)
                      aaCGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCa                                                                         <  2:1250035/100‑1 (MQ=255)
                      aaCGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCa                                                                         <  1:154509/100‑1 (MQ=255)
                            gtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTg                                                                   >  2:1046555/1‑100 (MQ=255)
                            gtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTg                                                                   <  2:2225920/100‑1 (MQ=255)
                             tggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGa                                                                  <  2:634559/100‑1 (MQ=255)
                              gggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGAt                                                                 <  2:394068/100‑1 (MQ=255)
                              gggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGAt                                                                 <  1:2653832/100‑1 (MQ=255)
                              gggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGAt                                                                 <  1:1466197/100‑1 (MQ=255)
                              gggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGAt                                                                 <  2:1540018/100‑1 (MQ=255)
                                gTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaa                                                               <  1:2348412/100‑1 (MQ=255)
                                gTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaa                                                               <  1:1037050/100‑1 (MQ=255)
                                 tAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaaa                                                              >  2:174830/1‑100 (MQ=255)
                                  aaTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaaaa                                                             >  2:1096866/1‑100 (MQ=255)
                                  aaTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaaaa                                                             >  2:1100767/1‑100 (MQ=255)
                                   aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                            >  1:83306/1‑100 (MQ=255)
                                    tGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACt                                                           >  2:1378548/1‑100 (MQ=255)
                                      cgcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGc                                                         >  1:869136/1‑100 (MQ=255)
                                          ggCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGAt                                                     <  2:2823296/100‑1 (MQ=255)
                                           gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                    <  1:884560/100‑1 (MQ=255)
                                            cGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCa                                                   >  2:1522698/1‑100 (MQ=255)
                                            cGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGGGAAGTGCTGGGCAAATTTGATAAAACTGCGGAGCa                                                   >  1:336313/1‑100 (MQ=255)
                                             ggTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaa                                                  <  2:966929/100‑1 (MQ=255)
                                               tGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaata                                                <  1:34105/100‑1 (MQ=255)
                                                gCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataa                                               >  1:13227/1‑100 (MQ=255)
                                                gCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataa                                               >  1:1160379/1‑100 (MQ=255)
                                                 cTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                              >  2:715223/1‑100 (MQ=255)
                                                 cTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                              >  2:2723873/1‑100 (MQ=255)
                                                 cTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                              <  2:2905208/100‑1 (MQ=255)
                                                  tGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAAtt                                             >  2:87738/1‑100 (MQ=255)
                                                   ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                            >  2:4666/1‑100 (MQ=255)
                                                     tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                          >  1:412095/1‑100 (MQ=255)
                                                     tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                          >  2:76587/1‑100 (MQ=255)
                                                      gATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAg                                         <  1:2119133/100‑1 (MQ=255)
                                                      gATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAGAACTGCGGATCAATAATTGAAg                                         <  1:1688019/100‑1 (MQ=255)
                                                          gCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTg                                     >  1:1319864/1‑100 (MQ=255)
                                                           ccAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGc                                    >  1:2388322/1‑100 (MQ=255)
                                                           ccAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGc                                    >  1:249876/1‑100 (MQ=255)
                                                           ccAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGc                                    <  2:518873/100‑1 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                   <  1:1618505/100‑1 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                   >  2:2831935/1‑100 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                   >  2:639923/1‑100 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                   >  1:1570883/1‑100 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                   >  2:1630974/1‑100 (MQ=255)
                                                             aaGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCg                                  <  2:126378/100‑1 (MQ=255)
                                                                 gggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAACCTGCGGATCAATAATTGAAGATTGCCGGCGa                              <  1:2187207/100‑1 (MQ=255)
                                                                 gggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGa                              <  2:552268/100‑1 (MQ=255)
                                                                 gggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGa                              <  1:937605/100‑1 (MQ=255)
                                                                  ggAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGat                             >  2:1272241/1‑100 (MQ=255)
                                                                     acacAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATc                          <  2:2042100/100‑1 (MQ=255)
                                                                     acacAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATc                          <  2:770686/100‑1 (MQ=255)
                                                                     acacAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATc                          <  2:656915/100‑1 (MQ=255)
                                                                      cacaAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATcc                         >  2:2239209/1‑100 (MQ=255)
                                                                      cacaAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGAGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATc                          >  1:897350/1‑99 (MQ=255)
                                                                       acaAAGTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGGTAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCa                        <  1:1875673/100‑1 (MQ=255)
                                                                        caAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAc                       >  2:1111725/1‑100 (MQ=255)
                                                                        caAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAc                       >  1:262685/1‑100 (MQ=255)
                                                                          aaTTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAccc                     >  1:2189268/1‑100 (MQ=255)
                                                                            tttGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCgg                   <  1:15470/100‑1 (MQ=255)
                                                                             ttGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGc                  <  2:199682/100‑1 (MQ=255)
                                                                              tGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGTGATATCCACCCGGCa                 >  2:2876407/1‑100 (MQ=255)
                                                                              tGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCa                 <  2:1335620/100‑1 (MQ=255)
                                                                              tGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCa                 <  1:415725/100‑1 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg              >  2:2609327/1‑100 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg              >  2:131559/1‑100 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg              <  2:815580/100‑1 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg              >  1:944932/1‑100 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg              <  1:156273/100‑1 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg              >  2:2346418/1‑100 (MQ=255)
                                                                                   gTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtgtg            >  2:661161/1‑100 (MQ=255)
                                                                                    tAAGAACGCGCTAACTTATGAGCGTGAAGTGCTGCGCAAATTTTATAATACTGCGGATCAATAATTGAATATTGCCGCGGATATCCTCCCGGCAAtgtg            <  1:1252682/99‑1 (MQ=255)
                                                                                    tAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtgtgt           >  2:1591107/1‑100 (MQ=255)
                                                                                     aaGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtgtgtg          >  1:1325456/1‑100 (MQ=255)
                                                                                      aGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGa         >  1:93856/1‑100 (MQ=255)
                                                                                      aGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGa         >  1:2457174/1‑100 (MQ=255)
                                                                                       gAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGaa        >  1:595376/1‑100 (MQ=255)
                                                                                        aaaGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAAt       >  1:2229745/1‑100 (MQ=255)
                                                                                           gcgcTGAGTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGcc    >  2:1533737/1‑100 (MQ=255)
                                                                                             gcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTg  <  2:1572254/100‑1 (MQ=255)
                                                                                             gcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTg  <  1:2228039/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTG  >  NC_000913/4295647‑4295839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑