Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,312,074 C→T 18.5% intergenic (‑32/+27) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,0740CT18.5% 330.4 / 59.8 150intergenic (‑32/+27)alsB/alsRD‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
Reads supporting (aligned to +/- strand):  ref base C (59/63);  new base T (7/21);  total (66/84)
Fisher's exact test for biased strand distribution p-value = 3.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

Only 100 of 153 total aligned reads displayed.

GCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCA  >  NC_000913/4311975‑4312173
                                                                                                   |                                                                                                   
gcaAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAAc                                                                                                     >  1:80954/1‑100 (MQ=255)
 caAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACt                                                                                                    >  2:2295304/1‑100 (MQ=255)
         cTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATCTTTATTCATAATAATGTTGTCACGGTAAGGAGACAGGTGACTc                                                                                                   >  1:231752/1‑93 (MQ=255)
           ggTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATgtc                                                                                          >  1:725949/1‑98 (MQ=255)
           ggTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGc                                                                                          >  2:1380361/1‑100 (MQ=255)
           ggTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTATCACGGTAAGGGGACAGGTAACTCCGCGATGGc                                                                                          <  1:1898014/100‑1 (MQ=255)
            gTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCg                                                                                         >  2:876961/1‑100 (MQ=255)
            gTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCg                                                                                         >  2:1745881/1‑100 (MQ=255)
            gTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGAGATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGc                                                                                          <  1:1051432/99‑1 (MQ=255)
             ttGACAACATTAAGCTCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCgg                                                                                        <  2:2872188/100‑1 (MQ=255)
             ttGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCgg                                                                                        <  2:418369/100‑1 (MQ=255)
              tGACAACATTAAGCCGACGAGTGTGCCGCTGAAATATTTCAGCTATTTATTCATATTACTGTTCTCCCGGTAAGTGGAAAGGTAACTc                                                                                                   >  1:2552546/1‑88 (MQ=255)
               gACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCg                                                                                         >  1:2602679/1‑97 (MQ=255)
                 caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAtt                                                                                    >  2:1885797/1‑100 (MQ=255)
                 caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAtt                                                                                    >  2:2786734/1‑100 (MQ=255)
                 caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAtt                                                                                    <  2:1564339/100‑1 (MQ=255)
                 caacaTTAAGCCCACGAGTGTGCCGCTGAAATATCTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAt                                                                                     >  1:1261087/1‑99 (MQ=255)
                   acaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAc                                                                                  >  1:526501/1‑100 (MQ=255)
                   acaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCCATCGCGGAGTTAc                                                                                  <  1:414860/100‑1 (MQ=255)
                    caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTAcc                                                                                 <  1:1997558/100‑1 (MQ=38)
                    caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                 <  2:2241605/100‑1 (MQ=255)
                    caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                 <  2:1161969/100‑1 (MQ=255)
                      ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                               <  1:695172/100‑2 (MQ=38)
                      ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                               <  1:2401/100‑2 (MQ=38)
                      ttAAGCCCACGAATGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                               <  1:1713720/100‑2 (MQ=38)
                       tAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgt                                                                              <  2:1049783/100‑3 (MQ=38)
                        aaGCCCTCGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtc                                                                             <  1:2466643/100‑4 (MQ=37)
                        aaGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtc                                                                             <  1:191112/100‑4 (MQ=37)
                        aaGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtc                                                                             <  1:2254802/100‑4 (MQ=37)
                        aaGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtc                                                                             <  2:562758/100‑4 (MQ=37)
                         aGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtcc                                                                            <  1:362169/100‑5 (MQ=37)
                          gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtccc                                                                           <  2:1196866/100‑6 (MQ=37)
                          gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                           >  1:1390069/1‑100 (MQ=255)
                           cccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCt                                                                          >  2:1350495/1‑100 (MQ=255)
                           cccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCt                                                                          >  1:218398/1‑100 (MQ=255)
                            ccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtcccc                                                                          <  1:1220010/99‑7 (MQ=25)
                             cACGAGTGTGCCGCTGAAATATTTCATATATTTAGTCAAAATAATCTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                               <  1:2669302/93‑2 (MQ=25)
                             cACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTa                                                                        >  2:981027/1‑100 (MQ=255)
                             cACGAGTGTGCCGCTCAAATATTTCATATATTTATTCATAATCATGTTCTCACGGTAAGGGGACAGCTAATTCCGCCCTCGCCGAGTTACCtg                                                                               <  1:2167223/93‑2 (MQ=255)
                               cGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAtt                                                                      <  1:2609834/100‑1 (MQ=255)
                               cGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAtt                                                                      <  1:206840/100‑1 (MQ=255)
                                 aGTGTGCCGCTGAAATATTTCAGATATTTAGTCATAATAAAGTGCTCACGGTAAGGGGACAGGTAACCCCGCGATGGTGGAATTACCTTTCTCTTATTTc                                                                    <  1:2577658/100‑1 (MQ=255)
                                  gtgtGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCAGCGATGTCGGAATTACCTTTTTCTTAtttta                                                                   >  1:2059219/1‑98 (MQ=255)
                                    gtgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgc                                                                 >  2:269399/1‑100 (MQ=255)
                                     tgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcg                                                                <  1:1222077/100‑1 (MQ=255)
                                     tgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcg                                                                >  2:2746669/1‑100 (MQ=255)
                                     tgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcg                                                                <  1:948768/100‑1 (MQ=255)
                                      gCCGCGGAAATATTTCAGTTATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcg                                                                <  1:847737/99‑1 (MQ=255)
                                       ccGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcg                                                              <  1:2789214/100‑1 (MQ=255)
                                          cTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCt                                                           >  2:378811/1‑100 (MQ=255)
                                              aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                       >  1:364364/1‑100 (MQ=255)
                                              aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                       <  2:1308076/100‑1 (MQ=255)
                                                   ttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaag                                                  >  1:1463969/1‑100 (MQ=255)
                                                    tCAGATATTTATTCATGATAATGTTCTCACGGTAAGTGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaaga                                                 <  1:1699226/100‑1 (MQ=255)
                                                    tCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaaga                                                 >  2:2833508/1‑100 (MQ=255)
                                                      aGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGaaa                                               >  2:2644312/1‑100 (MQ=255)
                                                      aGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGaaa                                               >  2:1950119/1‑100 (MQ=255)
                                                          atttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaa                                           <  1:1313740/100‑1 (MQ=255)
                                                           tttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaac                                          <  2:912273/100‑1 (MQ=255)
                                                           tttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaac                                          <  2:2575129/100‑1 (MQ=255)
                                                           tttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaac                                          >  2:582625/1‑100 (MQ=255)
                                                           tttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaac                                          <  1:1491758/100‑1 (MQ=255)
                                                            ttattCATAATAATGTTCTCACGGTAAGGGGGCAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaaca                                         <  2:1793182/100‑1 (MQ=255)
                                                            ttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaaca                                         <  2:24718/100‑1 (MQ=255)
                                                                tCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTg                                     <  1:1432024/100‑1 (MQ=255)
                                                                tCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTg                                     <  1:561624/100‑1 (MQ=255)
                                                                tCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTg                                     <  2:269557/100‑1 (MQ=255)
                                                                 cATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgc                                    >  1:1896229/1‑100 (MQ=255)
                                                                  ataataATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgcg                                   >  2:2578834/1‑100 (MQ=255)
                                                                   taataaTGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgcgc                                  >  2:144096/1‑100 (MQ=255)
                                                                    aataatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGcc                                 <  1:1953415/100‑1 (MQ=255)
                                                                           tactcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTTCCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGttttt                          >  2:1973743/3‑100 (MQ=255)
                                                                             ctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                        <  2:585912/100‑1 (MQ=255)
                                                                             ctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                        >  2:1370498/1‑100 (MQ=255)
                                                                             ctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                        <  2:1497988/100‑1 (MQ=255)
                                                                             ctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                        <  2:203695/100‑1 (MQ=255)
                                                                              tcACGGTCAGGAGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtttttt                         <  1:2310432/98‑1 (MQ=255)
                                                                               cACGGTAAGGGGACAGGTAACTCCGCGATGGCCGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCa                      >  2:2768813/1‑100 (MQ=255)
                                                                                  ggTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTTAGCTCGGCTGGTGAGAAGATATCTACAATTGCGCCGGTATTTTCCatat                   >  1:110449/1‑100 (MQ=255)
                                                                                  ggTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatat                   >  2:2504462/1‑100 (MQ=255)
                                                                                   gTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatatt                  <  2:915903/100‑1 (MQ=255)
                                                                                      aagaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaat               >  2:884531/8‑100 (MQ=25)
                                                                                       ggggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata              <  2:1807066/100‑1 (MQ=255)
                                                                                       agaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata              >  2:264735/7‑100 (MQ=25)
                                                                                       agaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata              >  1:2667633/7‑100 (MQ=37)
                                                                                        gggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatat             <  2:589741/100‑1 (MQ=255)
                                                                                          gACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGTTTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatatn            <  1:2812738/99‑2 (MQ=255)
                                                                                            aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc         >  2:696687/2‑100 (MQ=38)
                                                                                              ggTAACTCCGCGATGGCGGAATTACCTTTTTTTTTTTTTTTTTCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt       >  1:2382122/1‑100 (MQ=255)
                                                                                              ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt       >  2:417400/1‑100 (MQ=255)
                                                                                                tAACTCCGCGATGGCGGAATTGCCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt     <  1:465938/100‑1 (MQ=255)
                                                                                                tAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt     <  1:2644296/100‑1 (MQ=255)
                                                                                                tAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt     >  1:669678/1‑100 (MQ=255)
                                                                                                tAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt     <  2:669681/100‑1 (MQ=255)
                                                                                                tAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt     <  1:2261745/100‑1 (MQ=255)
                                                                                                 aaCTCCGCGATGGCGGAATTACTTTTTTCTTATTTCTGCGTGCCTGGTAAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGtt    <  1:2422530/100‑1 (MQ=255)
                                                                                                 aaCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGtt    <  2:67237/100‑1 (MQ=255)
                                                                                                 aaCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGtt    <  2:80955/100‑1 (MQ=255)
                                                                                                  aCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTc   <  2:2854486/100‑1 (MQ=255)
                                                                                                   cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCa  >  2:1440435/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCA  >  NC_000913/4311975‑4312173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: