Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 317,571 T→C 18.2% intergenic (+4/+155) ykgQ → / ← rclC pseudogene, putative dehydrogenase/reactive chlorine species (RCS) stress resistance inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913317,5710TC18.2% 227.5 / 47.5 110intergenic (+4/+155)ykgQ/rclCpseudogene, putative dehydrogenase/reactive chlorine species (RCS) stress resistance inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (51/39);  new base C (12/8);  total (63/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.70e-01

Only 100 of 114 total aligned reads displayed.

ATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATC  >  NC_000913/317472‑317670
                                                                                                   |                                                                                                   
aTTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATAtt                                                                                                     >  2:1358457/1‑100 (MQ=255)
   aGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCATGCTGTAAAAATACATGATTGATATTAAt                                                                                                  >  1:1747296/1‑100 (MQ=255)
      tCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCAt                                                                                               <  2:835738/100‑1 (MQ=255)
        gCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGt                                                                                             >  2:823845/1‑100 (MQ=255)
           tGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTAtt                                                                                          >  1:1194824/1‑100 (MQ=255)
             aTTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTAtttt                                                                                        >  2:1621010/1‑100 (MQ=255)
             aTTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTAtttt                                                                                        <  2:1627401/100‑1 (MQ=255)
             aTTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTAATATCAATCATGTAtttt                                                                                        <  1:2022740/100‑1 (MQ=255)
               ttGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTAc                                                                                      <  1:1537646/100‑1 (MQ=255)
               ttGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTAc                                                                                      <  2:1563553/100‑1 (MQ=255)
                tGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTAcc                                                                                     <  1:2183668/100‑1 (MQ=255)
                 gggTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTAATATCAATCATGTAtttttaca                                                                                    <  2:1189847/100‑5 (MQ=38)
                 gggTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTAATGTAAATATCCATGATTGATATTAATCATGTATTTTACCt                                                                                    >  1:917001/1‑100 (MQ=255)
                 gggTGATGTTGGGGAGTCGGTACAACATTTTAATCCTGAGTTTGTTCGTGGTTGTCTTGCTGTCAACATACATAATTGATATTAATCATGTAttttgac                                                                                     >  1:2200451/1‑96 (MQ=255)
                  ggTTATGTTGGGGAGTCGGTAAAACATTTTAATACTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTAATATCAATCATGTAtttttacag                                                                                   <  1:2683087/100‑6 (MQ=37)
                   gTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTaa                                                                                  >  2:1504218/1‑100 (MQ=255)
                   gTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTAATATCAATCATGTAtttttacagc                                                                                  <  2:949235/100‑7 (MQ=37)
                   gTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTAATATCAATCATGTAtttttacagc                                                                                  <  1:1812351/100‑7 (MQ=37)
                   gTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTAATATCAATCATGTAtttttacag                                                                                   <  1:443961/99‑6 (MQ=37)
                   gTTATGTTGGGGAGTCGGTAAAACATTTTAATACTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTAATATCAATCATGTAtttttacag                                                                                   <  1:444267/99‑6 (MQ=37)
                      aTGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAgac                                                                               >  2:2387855/1‑100 (MQ=255)
                        gTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAgacga                                                                             >  1:2354329/1‑100 (MQ=255)
                        gTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAgacga                                                                             >  1:1575349/1‑100 (MQ=255)
                        gTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAgacga                                                                             >  2:904944/1‑100 (MQ=255)
                        gTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAgacga                                                                             >  1:2768831/1‑100 (MQ=255)
                        gTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTGGATGTAAAAATACATGATTGAGATTAATCATGTATTTTACGTAAgacg                                                                              >  1:2703465/1‑99 (MQ=255)
                         ttGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAgacgac                                                                            <  2:2344053/100‑1 (MQ=255)
                           ggggAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACaa                                                                          >  2:481663/1‑100 (MQ=255)
                             ggAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACaaaa                                                                        >  2:1289782/1‑100 (MQ=255)
                              gAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACaaaaa                                                                       <  1:904941/100‑1 (MQ=255)
                               aGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAAc                                                                      <  2:1400547/100‑1 (MQ=255)
                                gTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAAcc                                                                     >  2:109325/1‑100 (MQ=255)
                                  cGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCtt                                                                   >  2:366873/1‑100 (MQ=255)
                                   ggTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTTCTGTAAAAATACATGATTGATCTTAATCATGTATTTTACCTAAGACGAAAAAAACCtat                                                                  >  1:1771084/1‑98 (MQ=255)
                                   ggTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCttt                                                                  >  2:1474029/1‑100 (MQ=255)
                                    gTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCtttt                                                                 <  2:490831/100‑1 (MQ=255)
                                      aaaaCATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTaa                                                               >  1:2501689/1‑100 (MQ=255)
                                      aaaaCATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTaa                                                               >  1:1647153/1‑100 (MQ=255)
                                       aaaCATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCAGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAAc                                                              >  2:1490144/1‑100 (MQ=255)
                                             tttAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAg                                                        <  1:976294/100‑1 (MQ=255)
                                                atctcTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTg                                                      <  1:115682/95‑1 (MQ=255)
                                                aaTCCTGAATTTGTTCGTGGTTGTCTTTCTTTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAATCCTTTTAACAGGGAGTGa                                                     <  1:416245/100‑1 (MQ=255)
                                                aaTCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGa                                                     <  2:751185/100‑1 (MQ=255)
                                                 attcTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGat                                                    <  1:324636/97‑1 (MQ=255)
                                                 aTCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGat                                                    >  2:324637/1‑100 (MQ=255)
                                                   ccTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGatat                                                  >  1:74993/1‑100 (MQ=255)
                                                    cTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGagatc                                                 >  2:115683/1‑99 (MQ=255)
                                                    cTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATAtt                                                 <  2:222912/100‑1 (MQ=255)
                                                    cTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATAtt                                                 >  2:406241/1‑100 (MQ=255)
                                                    cTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATAtt                                                 <  1:111637/100‑1 (MQ=255)
                                                    cTGAATTTGTTCATGGTTGTCTTGCTGTAAAAATACATGATTGATATTAAGCATATATTTTACCTAATACGACAAAAACCTTTTAAAAGGGAGAGATAtt                                                 >  1:2756216/1‑100 (MQ=255)
                                                      gAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGa                                               >  2:1850087/1‑100 (MQ=255)
                                                       aaaatGTTCGTGGTTGTCTTGCTGTAAAAATACATAATTGATATTAATCATGTATTTTAAAAAAAAAAAAAAAAACCTTTTAACAGGGAGTGata                                                   <  1:1787865/91‑1 (MQ=255)
                                                        aTTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATc                                             <  2:2107171/100‑1 (MQ=255)
                                                        aTTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATc                                             <  1:1716592/100‑1 (MQ=255)
                                                         tttGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCt                                            <  2:676162/100‑1 (MQ=255)
                                                         tttGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCt                                            >  2:2556773/1‑100 (MQ=255)
                                                           tGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTc                                          >  2:2515786/1‑100 (MQ=255)
                                                            gTTCGTGGTTGTCTTGCTGTAAAAATACATTATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACACGGAGTGATATTGATCTTCa                                         <  1:709048/100‑1 (MQ=255)
                                                             ttCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCAc                                        <  2:1505571/100‑1 (MQ=255)
                                                               cGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCActc                                      <  2:817305/100‑1 (MQ=255)
                                                               cGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCActc                                      <  2:375481/100‑1 (MQ=255)
                                                                 tGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTg                                    >  1:1092027/1‑100 (MQ=255)
                                                                   gTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTc                                  <  2:2159024/100‑1 (MQ=255)
                                                                       cgtcttaggtaaaaTACATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCatat                              >  2:1782305/11‑100 (MQ=25)
                                                                        gtcttaggtaaaaTACATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATAtc                             >  2:2736307/10‑100 (MQ=25)
                                                                        cTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACCGGGAGTGATATTGATCTTCACTCTGTCATAtc                             <  2:2768849/100‑1 (MQ=255)
                                                                         aaaaaaaaaaaaTACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCa                                 <  1:2542976/88‑1 (MQ=255)
                                                                           gCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTcc                          >  2:1227310/1‑100 (MQ=255)
                                                                            cTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCg                         >  2:1877975/1‑100 (MQ=255)
                                                                            cTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCg                         >  1:2411366/1‑100 (MQ=255)
                                                                             tGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCgg                        >  2:2217689/1‑100 (MQ=255)
                                                                             tGTAAAAAGACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACGCTGTCATATCTCCgg                        <  1:252826/100‑1 (MQ=255)
                                                                              ggtaaaaTACATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGATTGATATTGATCTTCACTCTGTCATATCTCCGGt                       >  1:2767300/4‑100 (MQ=37)
                                                                              ggtaaaaTACATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGt                       >  2:1212325/4‑100 (MQ=38)
                                                                               tAAAAATAAATGATTGATATTAATCATGTATTTTAAAAAAAAAAAAAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGt                       <  1:32712/99‑1 (MQ=255)
                                                                               gtaaaaTACATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTACCAGAGACTGATATTGAACTTCACTCTGTCATATCTACGGt                       >  1:410923/3‑99 (MQ=25)
                                                                               gtaaaaTACATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTa                      >  2:2837718/3‑100 (MQ=255)
                                                                               gtaaaaTACATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTa                      >  2:1496989/3‑100 (MQ=255)
                                                                               gtaaaaTACATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCCCCGGTa                      >  1:667594/3‑100 (MQ=38)
                                                                                 aaaaTACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAat                    >  1:2008743/1‑100 (MQ=255)
                                                                                 aaaaTACATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAat                    >  1:2486122/1‑100 (MQ=255)
                                                                                     tACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATgg                <  2:1826799/100‑1 (MQ=255)
                                                                                      aCATGATTAATATCAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGc               >  2:2297937/1‑100 (MQ=255)
                                                                                       cATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAatat                  >  1:2891343/1‑96 (MQ=255)
                                                                                        aTGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGt             >  2:1616037/1‑100 (MQ=255)
                                                                                         tgattgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTc            >  2:1011911/1‑100 (MQ=255)
                                                                                          gattgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCa           <  2:2393044/100‑1 (MQ=255)
                                                                                          gattgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCa           >  2:595882/1‑100 (MQ=255)
                                                                                          gattgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCa           >  2:2137708/1‑100 (MQ=255)
                                                                                          gattgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCa           <  2:2760597/100‑1 (MQ=255)
                                                                                          gattgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCa           <  2:2411381/100‑1 (MQ=255)
                                                                                           attgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAg          >  1:1464610/1‑100 (MQ=255)
                                                                                             tgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGc        <  1:2464802/100‑1 (MQ=255)
                                                                                             tgatATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGc        >  2:749805/1‑100 (MQ=255)
                                                                                               atatTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCtt      >  2:2161153/1‑100 (MQ=255)
                                                                                               atatTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCtt      <  2:1623220/100‑1 (MQ=255)
                                                                                                tatTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTtc     >  2:1633710/1‑100 (MQ=255)
                                                                                                  ttAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTtcat   >  2:1217994/1‑100 (MQ=255)
                                                                                                   tAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTtcatc  <  1:749802/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATTAGTTCGCTTGATTTGGGTTATGTTGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGATATTAATCATGTATTTTACCTAAGACGACAAAAACCTTTTAACAGGGAGTGATATTGATCTTCACTCTGTCATATCTCCGGTAATATGGCGTCAGGCTTCATC  >  NC_000913/317472‑317670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: