Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,365,453 A→C 15.8% intergenic (‑97/+19) cutA ← / ← dcuA divalent‑cation tolerance protein, copper sensitivity/C4‑dicarboxylate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,365,4530AC15.8% 897.7 / 158.9 377intergenic (‑97/+19)cutA/dcuAdivalent‑cation tolerance protein, copper sensitivity/C4‑dicarboxylate antiporter
Reads supporting (aligned to +/- strand):  ref base A (175/142);  new base C (34/26);  total (209/168)
Fisher's exact test for biased strand distribution p-value = 8.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.31e-01

Only 100 of 391 total aligned reads displayed.

CATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATG  >  NC_000913/4365354‑4365552
                                                                                                   |                                                                                                   
  tAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGaa                                                                                                    >  1:1283155/1‑99 (MQ=255)
  tAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAAc                                                                                                   >  1:233123/1‑100 (MQ=255)
  tAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAAc                                                                                                   >  2:2360412/1‑100 (MQ=255)
  tAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAAc                                                                                                   <  2:2230433/100‑1 (MQ=255)
    aaCACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACg                                                                                                  <  1:2024618/99‑1 (MQ=255)
     acacCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGcc                                                                                                >  1:2557674/1‑100 (MQ=255)
      cacCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGcc                                                                                                >  1:1672318/1‑99 (MQ=255)
        ccTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCcg                                                                                             >  2:767873/1‑100 (MQ=255)
        ccTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCcg                                                                                             >  1:217951/1‑100 (MQ=255)
        ccTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCcg                                                                                             >  2:379976/1‑100 (MQ=255)
          tCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcgct                                                                                           <  1:1829647/100‑2 (MQ=255)
           cGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACCACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcgctt                                                                                          <  2:356435/100‑3 (MQ=255)
           cGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGa                                                                                          >  1:1084852/1‑100 (MQ=255)
            gCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcgcttt                                                                                         <  2:1791322/100‑4 (MQ=255)
             cAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcgctttc                                                                                        <  2:2886738/100‑5 (MQ=255)
             cAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGCCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGa                                                                                          >  1:1918402/1‑98 (MQ=255)
              aaGAGCAGAGGGAGCCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGAtt                                                                                        <  1:1986689/99‑1 (MQ=255)
              aaGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcgctttct                                                                                       <  1:282432/100‑6 (MQ=255)
              aaGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTTCACGCCCcgctttct                                                                                       <  1:1230917/100‑6 (MQ=255)
              aaGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGAtt                                                                                        <  1:828666/99‑1 (MQ=255)
              aaGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTg                                                                                       <  1:870296/100‑1 (MQ=255)
               aGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGa                                                                                      >  2:922264/1‑100 (MQ=255)
               aGAACAGATGGAAAAGAAAGAGAGAGGAAAAAAAACAACGGAGGGGGGAAGGATGTCAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGTCCGGGGATTg                                                                                       <  1:1616646/99‑1 (MQ=255)
                gAACAGATGGAAGCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGGGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGa                                                                                      <  1:101775/99‑1 (MQ=255)
                 aaCAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTGGGCGGGAAAGACAGCGGGGGGTGAACGCACCGAGATTGATc                                                                                    >  1:2771853/1‑100 (MQ=255)
                 aaCAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATc                                                                                    <  1:434795/100‑1 (MQ=255)
                 aaCAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATc                                                                                    <  2:1659472/100‑1 (MQ=255)
                 aaCAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATc                                                                                    <  2:139352/100‑1 (MQ=255)
                   cAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCa                                                                                   >  1:601162/1‑99 (MQ=255)
                    aGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCAtt                                                                                 >  2:1198812/1‑100 (MQ=255)
                     gATGGAGACGCAGGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCAt                                                                                  <  1:2432527/98‑1 (MQ=255)
                     gATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCAtt                                                                                 <  1:1387701/99‑1 (MQ=255)
                      aTGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTAc                                                                               <  2:2074539/100‑1 (MQ=255)
                       tGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGACAGAAAACGGGGCGTGAACGCCCCGCGATTGATGATTACa                                                                              >  1:1766892/1‑100 (MQ=255)
                       tGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACa                                                                              >  1:285453/1‑100 (MQ=255)
                        ggAACCGCAAAAGGGAGGTCACTATACAACGCACGCGGGAAGGATGCTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGTCCCGCGATTGATCATTACAg                                                                             <  1:1042236/100‑1 (MQ=255)
                          aaCCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCa                                                                           <  1:1580163/100‑1 (MQ=255)
                            ccGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCa                                                                           <  1:319090/98‑1 (MQ=255)
                            ccGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATg                                                                         >  1:150943/1‑100 (MQ=255)
                              gCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGaa                                                                       >  2:1268801/1‑100 (MQ=255)
                              gCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGaa                                                                       >  2:1121375/1‑100 (MQ=255)
                              gCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGaa                                                                       <  1:204394/100‑1 (MQ=255)
                                aaGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGc                                                                     <  2:273594/100‑1 (MQ=255)
                                    gagagGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTAcc                                                                 >  2:2307907/1‑100 (MQ=255)
                                       agGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAg                                                              <  1:922261/100‑1 (MQ=255)
                                        ggTCAGTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGACAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAg                                                              <  1:362568/99‑1 (MQ=255)
                                        ggTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGc                                                             >  1:2395827/1‑100 (MQ=255)
                                        ggTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGc                                                             >  1:2130740/1‑100 (MQ=255)
                                        ggTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGc                                                             <  2:1557505/100‑1 (MQ=255)
                                         gTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTCATCATTACAGCATGAAGCTACccacca                                                            >  1:247/1‑100 (MQ=255)
                                           cACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGCTTGATCATTACAGCATGAAGCTACCCAGCAcg                                                          >  2:445162/1‑100 (MQ=255)
                                           cACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcg                                                          >  2:1049707/1‑100 (MQ=255)
                                                taCAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagcc                                                     <  1:1478227/100‑1 (MQ=255)
                                                 aCAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccg                                                    >  2:1703669/1‑100 (MQ=255)
                                                  cAACGGACGGGGGAAGGCTGTTAGTCGGCGGTAAAGAAATCGGGGCGAGAACGGCCCGCGATAGAACATTACAGCGCGAAGGTACCAATGAcgaagc                                                      >  1:2584785/1‑97 (MQ=255)
                                                  cAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACACAGCAcgaagccga                                                   >  2:851191/1‑100 (MQ=255)
                                                   aaCGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccgaa                                                  >  2:1931647/1‑100 (MQ=255)
                                                    acggagggGGAAAGGATGTTAGACGGCCGGAAAGAAAGCGGGGCGTTAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccgaag                                                 <  1:2145952/100‑1 (MQ=255)
                                                     cggacggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccgaagc                                                <  1:1032019/100‑1 (MQ=255)
                                                       gacggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCaa                                              <  1:1072493/100‑1 (MQ=255)
                                                         cggGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAAc                                            >  2:539319/1‑100 (MQ=255)
                                                           ggggAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCAcgaagccgaagctccgat                                          >  2:1023578/1‑94 (MQ=255)
                                                           ggggAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACgg                                          <  2:885726/100‑1 (MQ=255)
                                                           ggggAAGGATGTTAGTAGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACgg                                          <  1:2377168/100‑1 (MQ=255)
                                                            gggAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGc                                         >  2:2150110/1‑100 (MQ=255)
                                                              gAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGc                                         >  1:775335/1‑98 (MQ=255)
                                                              gAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCa                                       >  1:2654246/1‑100 (MQ=255)
                                                               aaGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAg                                      <  1:1165308/100‑1 (MQ=255)
                                                                aGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAgg                                     >  2:1643690/1‑100 (MQ=255)
                                                                aGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAgg                                     <  2:1867864/100‑1 (MQ=255)
                                                                 ggATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAggg                                    >  2:1783017/1‑100 (MQ=255)
                                                                  gATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACGGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAgggn                                   <  2:281633/100‑2 (MQ=255)
                                                                  gATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGc                                   <  2:2010879/100‑1 (MQ=255)
                                                                  gATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGc                                   <  2:2180669/100‑1 (MQ=255)
                                                                   aTGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCa                                  <  2:261762/100‑1 (MQ=255)
                                                                    tGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCaa                                 >  2:2502940/1‑100 (MQ=255)
                                                                        aGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACAcc                             >  1:2695834/1‑100 (MQ=255)
                                                                          tCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCa                           <  2:1598457/100‑1 (MQ=255)
                                                                           cggcggGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCag                          >  2:1081447/1‑100 (MQ=255)
                                                                             gcggGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCagag                        <  2:2425359/100‑1 (MQ=255)
                                                                             gcggGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCagag                        <  1:2256597/100‑1 (MQ=255)
                                                                                ggAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTAc                     >  2:2851762/1‑100 (MQ=255)
                                                                                 gaaagaaaGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTAcc                    <  1:1649077/100‑1 (MQ=255)
                                                                                  atcaatcgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTAccc                   >  2:585054/8‑100 (MQ=38)
                                                                                  aaagaaagCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTAccc                   >  2:225232/1‑100 (MQ=255)
                                                                                   aagaaagCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCg                  <  1:174391/100‑1 (MQ=255)
                                                                                     gaaagCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCggg                >  2:2662262/1‑100 (MQ=255)
                                                                                     aatcgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCggg                >  1:990610/5‑100 (MQ=255)
                                                                                      atcgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGa               >  2:1165736/4‑100 (MQ=255)
                                                                                      atcgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGa               >  1:525368/4‑100 (MQ=255)
                                                                                      atcgCGGGGCGTTCACGCCCCGCGATGGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAAAACCCAGCATACACGGGa               >  1:1104264/4‑100 (MQ=255)
                                                                                       aagCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGAt              <  1:403022/100‑1 (MQ=255)
                                                                                        cgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATg             >  2:146417/2‑100 (MQ=255)
                                                                                        agCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATg             >  2:246591/1‑100 (MQ=255)
                                                                                          cGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGACGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGAt              >  1:746112/1‑97 (MQ=255)
                                                                                           ggggCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCCGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATgaag          >  1:677369/1‑100 (MQ=255)
                                                                                             ggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATgaagaa        >  1:1192685/1‑100 (MQ=255)
                                                                                             ggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATgaagaa        >  2:498558/1‑100 (MQ=255)
                                                                                               cGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCATAGTACCCGGGATGAAGAACg      <  1:490050/100‑1 (MQ=255)
                                                                                                 tGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGa    >  2:1217099/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CATAAACACCTCGCAAGAACAGATGGAACCGCAAGAGAGAGGTCACTATACAACGGACGGGGGAAGGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATG  >  NC_000913/4365354‑4365552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: