Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,280 G→A 15.1% intergenic (+15/‑37) efp → / → ecnA polyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,2800GA15.1% 342.7 / 41.4 138intergenic (+15/‑37)efp/ecnApolyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B
Reads supporting (aligned to +/- strand):  ref base G (62/55);  new base A (11/10);  total (73/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 144 total aligned reads displayed.

GGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACC  >  NC_000913/4376181‑4376379
                                                                                                   |                                                                                                   
 gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCgg                                                                                                    >  1:716749/1‑100 (MQ=255)
 gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCgg                                                                                                    <  1:368360/100‑1 (MQ=255)
  ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGc                                                                                                   >  2:659207/1‑100 (MQ=255)
  ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGc                                                                                                   >  1:1638346/1‑100 (MQ=255)
   tAAAGTTCCGCTGTTTGTACAAATCGGCGAAGGCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGcc                                                                                                  <  1:1003232/100‑1 (MQ=255)
    aaaGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGGTACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCt                                                                                                 <  1:2507738/100‑1 (MQ=255)
    aaaGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCt                                                                                                 >  1:1574542/1‑100 (MQ=255)
     aaGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGAGGCCt                                                                                                 >  1:1899942/1‑99 (MQ=255)
      aGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGc                                                                                               <  2:1436092/100‑1 (MQ=255)
       gTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCa                                                                                              >  2:533436/1‑100 (MQ=255)
         tCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAgg                                                                                            >  1:254332/1‑100 (MQ=255)
            gCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTg                                                                                         <  2:2392291/100‑1 (MQ=255)
             cTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGc                                                                                        >  1:2418007/1‑100 (MQ=255)
              tGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCa                                                                                       <  1:502500/100‑1 (MQ=255)
               gTTTGTACAAATCGGCGAAGTCATCAAAGTGGCTACCCGCTCTGGTGAATACGTCTCTCGCGTTAAGTAATGCGGTTGTGGTGCAGCCTGCAGGACGCAc                                                                                      <  1:1394650/100‑1 (MQ=255)
               gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCa                                                                                       <  2:162360/99‑1 (MQ=255)
               gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAc                                                                                      <  2:2293313/100‑1 (MQ=255)
                tttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAcc                                                                                     <  1:2029915/100‑1 (MQ=255)
                 ttGTACAGATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCACCa                                                                                    <  1:1716248/100‑1 (MQ=255)
                 ttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCACCa                                                                                    <  1:1023394/100‑1 (MQ=255)
                  tGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCAt                                                                                   >  1:1986433/1‑100 (MQ=255)
                    tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                 <  2:2779211/100‑1 (MQ=255)
                     aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                >  2:1624684/1‑100 (MQ=255)
                      cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACt                                                                               <  2:318262/100‑1 (MQ=255)
                      cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACt                                                                               >  1:1293391/1‑100 (MQ=255)
                      cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGAAGGCTGCACCATCACt                                                                               >  1:2110623/1‑100 (MQ=255)
                       aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACtt                                                                              >  2:2803663/1‑100 (MQ=255)
                       aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACtt                                                                              >  2:546977/1‑100 (MQ=255)
                            ggggAAGTCATCAAAGTGGATACCCGCTCGGTGGAATACGTCGCTCGCGTTAAGTAATGCGGTTTTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                         <  1:1011/97‑1 (MQ=255)
                            ggCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                         >  1:2141039/1‑100 (MQ=255)
                             gCGAAGTCATCAAAGTGTATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAtt                                                                           <  1:563384/97‑1 (MQ=255)
                             gCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                        <  2:108040/100‑1 (MQ=255)
                             gCGAAGTCATCAAAGTGGATACCCGCACTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAATCa                                                                         >  2:2518833/1‑99 (MQ=255)
                              cGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                        <  2:142588/99‑1 (MQ=255)
                              cGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTATCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAga                                                                       >  1:142587/1‑99 (MQ=255)
                                    catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                 >  1:2663558/1‑100 (MQ=255)
                                    catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                 >  2:381885/1‑100 (MQ=255)
                                    catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCaga                                                                  >  1:507496/1‑99 (MQ=255)
                                      tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgat                                                               >  2:332727/1‑100 (MQ=255)
                                             ggATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGaaa                                                        >  1:2148380/1‑100 (MQ=255)
                                              gATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAAc                                                       >  2:2842587/1‑100 (MQ=255)
                                               aTACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACg                                                      <  1:301089/100‑1 (MQ=255)
                                                 aCCCTCTCTGGTGTATACGTCTCTCGCGTAAACTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGATATGATGAAACg                                                      <  1:1528202/98‑1 (MQ=255)
                                                  cccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                   >  1:1347570/1‑100 (MQ=255)
                                                  cccGATCTGGTGAATCCGTCTCTCGCGTGACGTAATGCGGTTGTGGTGCGTCCTGCTGGCTGCACTATCACTTATTCAGGTCAGAGAtgatga                                                          >  1:2524053/1‑93 (MQ=255)
                                                    cGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTa                                                 >  2:1382192/1‑100 (MQ=255)
                                                     gCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTAt                                                >  1:449687/1‑100 (MQ=255)
                                                       tctGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCg                                              >  2:176602/1‑100 (MQ=255)
                                                        ctGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGt                                             >  1:1886224/1‑100 (MQ=255)
                                                          ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGtt                                            >  1:2084346/1‑99 (MQ=255)
                                                          ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                           >  1:2141969/1‑100 (MQ=255)
                                                          ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                           <  2:2526135/100‑1 (MQ=255)
                                                           gTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCt                                          <  2:1886233/100‑1 (MQ=255)
                                                             gAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTg                                        <  2:705775/100‑1 (MQ=255)
                                                             gAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTg                                        <  2:1725716/100‑1 (MQ=255)
                                                              aaTACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGt                                       <  2:2889115/100‑1 (MQ=255)
                                                                tACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGttt                                     <  2:1182789/100‑1 (MQ=255)
                                                                tACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGttt                                     >  1:2035485/1‑100 (MQ=255)
                                                                 aCGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtttt                                    <  1:2755601/100‑1 (MQ=255)
                                                                  cGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttg                                   <  1:478373/100‑1 (MQ=255)
                                                                  cGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttg                                   <  2:2148393/100‑1 (MQ=255)
                                                                   gTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgc                                  >  2:2115030/1‑100 (MQ=255)
                                                                   gTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgc                                  <  2:2141055/100‑1 (MQ=255)
                                                                   gTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgc                                  <  1:476100/100‑1 (MQ=255)
                                                                   gTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgc                                  >  1:86290/1‑100 (MQ=255)
                                                                     ctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                <  1:2712269/100‑1 (MQ=255)
                                                                     ctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                <  1:2259244/100‑1 (MQ=255)
                                                                      tctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                               >  1:676222/1‑100 (MQ=255)
                                                                       ctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttgc                              >  2:1573912/1‑100 (MQ=255)
                                                                         cgcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATTATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCa                            >  1:2826737/1‑100 (MQ=255)
                                                                          gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAg                           <  2:524804/100‑1 (MQ=255)
                                                                            gTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCa                         >  2:2337288/1‑100 (MQ=255)
                                                                            gTGAAGTAATGCGGTTGTGGTGCGGCATGAAGGCTGCAACATCACTTATTCAGGTAAGGGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCa                         <  1:1585803/100‑1 (MQ=255)
                                                                               aaGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgc                      >  2:2630226/1‑100 (MQ=255)
                                                                                   aaTGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctgct                  >  2:2133531/1‑100 (MQ=255)
                                                                                    aTGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTc                 <  2:1559192/100‑1 (MQ=255)
                                                                                    aTGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTc                 <  2:440286/100‑1 (MQ=255)
                                                                                     tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTTCCAGCACGCTGCTCa                >  2:1807240/1‑100 (MQ=255)
                                                                                     tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCa                >  2:172895/1‑100 (MQ=255)
                                                                                     ataagtgatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCa                >  1:2900279/9‑100 (MQ=38)
                                                                                      gCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCAc               >  1:1306153/1‑100 (MQ=255)
                                                                                       cGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACg              >  2:871823/1‑100 (MQ=255)
                                                                                        ggTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACgg             >  2:657241/1‑100 (MQ=255)
                                                                                        agtgatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTTACgg             >  2:2059570/6‑100 (MQ=38)
                                                                                        agtgatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACgg             >  2:2189837/6‑100 (MQ=38)
                                                                                         gTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACggg            >  2:2221141/1‑100 (MQ=255)
                                                                                          ttGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGc           <  1:1939718/100‑1 (MQ=255)
                                                                                          ttGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGc           <  1:2730032/100‑1 (MQ=255)
                                                                                           gatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt          >  1:846969/3‑100 (MQ=255)
                                                                                            gtggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGCTGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTg         <  2:726008/100‑1 (MQ=255)
                                                                                             tggtgCAGCCTGCAGGCCGCACGAGCGCTGAGTAGGGTCAGAGATGGTGGCACGCTTTATCTTTCTTGTTTTGCTTGCCTGCACCCTGCTCACAGGCTg         >  1:183072/1‑99 (MQ=255)
                                                                                              ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGtn       <  2:836415/100‑2 (MQ=255)
                                                                                              ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa       >  2:1122500/1‑100 (MQ=255)
                                                                                              ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa       <  2:1066049/100‑1 (MQ=255)
                                                                                              ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa       >  2:1406576/1‑100 (MQ=255)
                                                                                              ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa       <  2:2892462/100‑1 (MQ=255)
                                                                                              ggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa       >  2:1309812/1‑100 (MQ=255)
                                                                                                tgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTTACGGGCTGTAac     >  1:1494207/1‑100 (MQ=255)
                                                                                                  cGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAacac   <  1:2803645/100‑1 (MQ=255)
                                                                                                   ggCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACAcc  <  2:1441024/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACC  >  NC_000913/4376181‑4376379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: