Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 14,124 T→A 15.2% intergenic (+45/‑44) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091314,1240TA15.2% 348.7 / 49.4 138intergenic (+45/‑44)dnaK/dnaJchaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
Reads supporting (aligned to +/- strand):  ref base T (60/57);  new base A (13/8);  total (73/65)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

Only 100 of 142 total aligned reads displayed.

AAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGC  >  NC_000913/14026‑14222
                                                                                                  |                                                                                                  
aaaGATGAAGATGTTGTCGACGCTGAATTTGAAGAAAAAAAAAAAAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAt                                                                                                    <  1:2440182/99‑1 (MQ=255)
 aaGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAttt                                                                                                  >  2:2581726/1‑100 (MQ=255)
          aTGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATACACGGGTAATTATACTGACACGGGCGAAGGGGAATCTCCTCTCCGc                                                                                         >  1:430851/1‑100 (MQ=255)
             ttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCg                                                                                      <  2:307651/100‑1 (MQ=37)
             ttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCg                                                                                      <  2:1412306/100‑1 (MQ=255)
              tttCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGt                                                                                     <  1:2331258/98‑1 (MQ=37)
               gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTg                                                                                    <  1:2715215/100‑1 (MQ=37)
               gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTg                                                                                    >  2:2815372/1‑100 (MQ=255)
               gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTg                                                                                    <  1:1414013/100‑1 (MQ=255)
                 cGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTgtc                                                                                  <  2:745848/100‑3 (MQ=25)
                 cGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCa                                                                                  >  1:2073737/1‑100 (MQ=255)
                  gACGCTGAATTTGAAGAAGTCACAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCACCCCGCCCGTGCAt                                                                                 >  1:90936/1‑100 (MQ=255)
                  gACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAt                                                                                 >  2:2660845/1‑100 (MQ=255)
                   aCGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAtt                                                                                <  1:2509174/100‑1 (MQ=255)
                    cGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTc                                                                               >  1:2165368/1‑100 (MQ=255)
                     gCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCa                                                                              <  2:180810/100‑1 (MQ=255)
                     gCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCa                                                                              <  1:2446232/100‑1 (MQ=255)
                         aaTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTa                                                                          >  1:1546365/1‑100 (MQ=255)
                            ttaaagaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGCCACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAggg                                                                       <  2:369521/97‑1 (MQ=255)
                            ttGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAggg                                                                       <  2:1591428/100‑1 (MQ=255)
                            ttGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGACGCAGGGGACTATCCTCTCCGCCCGTGCCATCACCTAg                                                                         >  1:1164070/1‑98 (MQ=255)
                             tGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAgggg                                                                      >  1:2575458/1‑100 (MQ=255)
                             tGAAGAAGTCAAAAACAAAAAATAATCGCCCTCTAAACGGGTAATTATACTGACACGGGCGCATGGGAATTTCCTCTCCGCCCGTCCATTCCTCTAgggg                                                                      >  1:1207659/1‑100 (MQ=255)
                              gaagaaGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGc                                                                     <  1:2357059/100‑1 (MQ=255)
                              gaagaaGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGc                                                                     <  1:815076/100‑1 (MQ=255)
                              gaagaaGTCAAAGACAAAAAATAATCGCCCTAGAAACGGCGAATTATACTGTCACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAggg                                                                       <  1:559773/98‑1 (MQ=255)
                                agaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCa                                                                    >  2:344397/1‑99 (MQ=255)
                                 gaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAAt                                                                  <  2:2205323/100‑1 (MQ=255)
                                    gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTa                                                               >  1:632520/1‑100 (MQ=255)
                                    gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTa                                                               >  1:1295405/1‑100 (MQ=255)
                                        aaGAAAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaaa                                                           <  1:914855/100‑1 (MQ=255)
                                            cAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAt                                                       >  2:1153768/1‑100 (MQ=255)
                                            cAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAt                                                       <  2:1479887/100‑1 (MQ=255)
                                            cAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAt                                                       >  2:2690904/1‑100 (MQ=255)
                                            cAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAt                                                       >  2:1093049/1‑100 (MQ=255)
                                             aaaaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAg                                                         <  1:2571754/97‑1 (MQ=255)
                                               aaaaTAATCGCCCTATAACCGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGc                                                    <  1:2113775/100‑1 (MQ=255)
                                               aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGc                                                    <  2:723516/100‑1 (MQ=255)
                                                 aataatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTa                                                  <  1:350878/100‑1 (MQ=255)
                                                 aataatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTa                                                  <  2:1928992/100‑1 (MQ=255)
                                                   taatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAg                                                <  2:575521/100‑1 (MQ=255)
                                                    aatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGc                                               <  2:2283134/100‑1 (MQ=255)
                                                     atCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCa                                              <  1:2820412/100‑1 (MQ=255)
                                                      tCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCaa                                             <  2:446671/100‑1 (MQ=255)
                                                      tCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCaa                                             <  2:762179/100‑1 (MQ=255)
                                                       cGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCaa                                             >  1:2516669/1‑99 (MQ=255)
                                                       cGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAg                                            <  1:1313335/100‑1 (MQ=255)
                                                        gCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGa                                           >  2:2408608/1‑100 (MQ=255)
                                                         cccTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGat                                          <  2:1948689/100‑1 (MQ=255)
                                                         cccTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGa                                           >  1:684556/1‑99 (MQ=255)
                                                             ataAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGattat                                       <  1:1158454/99‑1 (MQ=255)
                                                              taAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAttatta                                     >  2:205878/1‑100 (MQ=255)
                                                                aaCGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACg                                   <  2:2645210/100‑1 (MQ=255)
                                                                  cGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACgag                                 >  1:825838/1‑100 (MQ=255)
                                                                    ggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACgaga                                >  2:1780332/1‑99 (MQ=255)
                                                                    ggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAt                               <  2:2058176/100‑1 (MQ=255)
                                                                    ggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAt                               <  1:945028/100‑1 (MQ=255)
                                                                     gTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGattagatcggaaga                              >  2:848705/1‑90 (MQ=255)
                                                                     gTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAtt                              >  1:527435/1‑100 (MQ=255)
                                                                         ttATACTGACACGGGCGAAGGGGAATTTTCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAg                          <  2:713993/100‑1 (MQ=255)
                                                                           ataCTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGc                        <  2:2541828/100‑1 (MQ=255)
                                                                            taCTGACACTGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCg                       <  1:95383/100‑1 (MQ=255)
                                                                            taCTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCg                       <  1:276072/100‑1 (MQ=255)
                                                                             aCTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGt                      >  1:659077/1‑100 (MQ=255)
                                                                              aatgcacGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAATATTACGAGACTTTAGGCg                       >  1:1608471/5‑98 (MQ=21)
                                                                               atgcacGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGttt                    >  1:1624495/4‑100 (MQ=25)
                                                                                tgcacGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTc                   >  2:563249/3‑100 (MQ=25)
                                                                                gACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGtt                     >  1:1637225/1‑98 (MQ=255)
                                                                                gACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATACATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTc                   >  1:1797857/1‑100 (MQ=255)
                                                                                 acacGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTcc                  <  2:108014/100‑1 (MQ=255)
                                                                                  cacGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCa                 >  1:201306/1‑100 (MQ=37)
                                                                                  cacGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCAGCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCa                 >  1:1929151/1‑100 (MQ=37)
                                                                                   acGGGCGGAGAGGAAATTCCCCTTCGCCCGTGTATTCATCTAAGGACAATGTCAAAAAGATGGCTAAGCAATCTAATTCCGAGATTAGAGGCGTTTCCaa                >  1:1810516/1‑100 (MQ=12)
                                                                                   acGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaa                >  2:945391/1‑100 (MQ=37)
                                                                                   acGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaa                <  2:1991087/100‑1 (MQ=255)
                                                                                   acGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaa                <  1:2700957/100‑1 (MQ=255)
                                                                                    cGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaa               >  2:1181839/1‑100 (MQ=37)
                                                                                    cGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaa               >  1:1714930/1‑100 (MQ=255)
                                                                                    cGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaa               >  1:802335/1‑100 (MQ=255)
                                                                                    cGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaa               <  1:2395583/100‑1 (MQ=255)
                                                                                     gggCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa              >  2:2672926/1‑100 (MQ=37)
                                                                                      ggCGGAGAGGAAATTCCCCTTCGCCCGTGTATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAAc             <  2:1426542/100‑1 (MQ=37)
                                                                                       gCGAAGTGTAATTTCCTCTCCGCCCGGGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAAc             <  1:735874/99‑1 (MQ=255)
                                                                                       gCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAAc             <  1:419950/99‑1 (MQ=255)
                                                                                       gCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACa            <  2:2573871/100‑1 (MQ=255)
                                                                                       gCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACa            >  1:2197548/1‑100 (MQ=255)
                                                                                       gCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACa            >  1:110761/1‑100 (MQ=255)
                                                                                          aaGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCTAAAACAGCg         <  2:1231855/100‑1 (MQ=255)
                                                                                          aaGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGAGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCg         <  1:1206731/100‑1 (MQ=255)
                                                                                           aGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATGTAAAAAAGATGGCTAAGCAAGAGTATTACGAGATTTTAGGCGTTTCCAAAACAGCgg        <  1:2820310/100‑1 (MQ=255)
                                                                                            ggggAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGa       >  2:429164/1‑100 (MQ=255)
                                                                                            ggggAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGa       <  2:476549/100‑1 (MQ=255)
                                                                                              ggAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAag     >  2:2772364/1‑100 (MQ=255)
                                                                                              ggAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAag     <  1:946296/100‑1 (MQ=255)
                                                                                              ggAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAag     >  2:1214313/1‑100 (MQ=255)
                                                                                               gAATTTCCTCTCCGCCCGTTCATTCATCTAGGGGCAATTTAAAAAAGATGTCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAag     <  1:196645/99‑1 (MQ=255)
                                                                                               gAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAaga    <  2:2280280/100‑1 (MQ=255)
                                                                                               gAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAaga    >  2:2178006/1‑100 (MQ=255)
                                                                                                naTTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAagag   >  2:1753620/2‑100 (MQ=255)
                                                                                                 aTTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGc  >  2:1038436/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
AAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGC  >  NC_000913/14026‑14222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: