Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 478,757 T→C 16.2% intergenic (‑140/+24) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913478,7570TC16.2% 268.8 / 43.3 122intergenic (‑140/+24)ylaB/ylaCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (57/45);  new base C (11/9);  total (68/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-01

Only 100 of 127 total aligned reads displayed.

GATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTT  >  NC_000913/478658‑478856
                                                                                                    |                                                                                                   
gATTGTGGAAAAAGGCTGGCTGGAGCTAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCtt                                                                                                     >  1:2368595/1‑100 (MQ=255)
gATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCtt                                                                                                     <  2:803703/100‑1 (MQ=255)
  ttctgGAAAAATGTTGGCTGGGGTGGAAGAGGTAGAGCTGGTATTACCTTAAGTAATGGGCTGGAAAAATAATTGTCATTTGACCAGGCTGAGAGCTTTa                                                                                                   <  1:1818007/97‑1 (MQ=255)
    gtgGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACg                                                                                                 >  1:2714565/1‑100 (MQ=255)
    gtgGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACg                                                                                                 >  2:553945/1‑100 (MQ=255)
    gtgGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACg                                                                                                 >  1:1820555/1‑100 (MQ=255)
     tgGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGc                                                                                                <  1:553942/100‑1 (MQ=255)
     tgGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGc                                                                                                >  2:517406/1‑100 (MQ=255)
      ggAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGcc                                                                                               >  2:1741436/1‑100 (MQ=255)
      ggAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGcc                                                                                               >  1:2391189/1‑100 (MQ=255)
       gAAAAAGGCTGGCTGGAGATAAGGAGGGGGAGCTGGTGTTTCATGAAGTAATGGGAAGAAACGATCATAGTTATTTGACCAGGCGAAGAGCTTTACGCCt                                                                                              >  1:877725/1‑100 (MQ=255)
        aaagaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACTATCATTGTCATTTGACCAGGCGCATAGCTTTACGCCTg                                                                                             <  1:1801747/100‑1 (MQ=255)
         aaaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTgg                                                                                            <  1:1292628/100‑1 (MQ=255)
          aaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTGGt                                                                                           <  2:2222516/100‑1 (MQ=38)
          aaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGt                                                                                           >  1:2522324/1‑100 (MQ=255)
          aaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGt                                                                                           <  2:2522339/100‑1 (MQ=255)
           aaGGTTTGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGt                                                                                           <  1:873004/99‑1 (MQ=255)
           aaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCTGGCGTAAAGCTCTGCGCCTGGtc                                                                                          <  1:1793690/100‑2 (MQ=255)
           aaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTGGtc                                                                                          <  2:164933/100‑2 (MQ=38)
           aaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTa                                                                                          <  2:2220290/100‑1 (MQ=255)
            aGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTGGtca                                                                                         <  1:1234385/100‑3 (MQ=38)
            aGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAt                                                                                         >  2:1342554/1‑100 (MQ=255)
                tggctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTGGtcaaatg                                                                                     <  2:1718990/100‑7 (MQ=37)
                 ggctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAc                                                                                    <  1:1610533/100‑1 (MQ=255)
                 ggctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAc                                                                                    >  1:1543999/1‑100 (MQ=255)
                  gctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAcc                                                                                   <  1:106454/100‑1 (MQ=255)
                   ctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccc                                                                                  >  1:1477357/1‑100 (MQ=255)
                   ctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccc                                                                                  >  1:1234347/1‑100 (MQ=255)
                     ggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTa                                                                                >  1:33167/1‑100 (MQ=255)
                     ggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTa                                                                                >  1:1064091/1‑100 (MQ=255)
                     ggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTa                                                                                >  2:1087716/1‑100 (MQ=255)
                       agaTAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCGTTACGCCTGGTATGTAACCCTAAc                                                                              <  1:2456383/100‑1 (MQ=255)
                          tAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTa                                                                           <  1:2509328/100‑1 (MQ=255)
                           aaGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTaa                                                                          <  2:2695264/100‑1 (MQ=255)
                             ggaggTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGa                                                                        >  1:2345785/1‑100 (MQ=255)
                             ggaggTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGa                                                                        <  2:1935661/100‑1 (MQ=255)
                                 gTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                                                    <  2:1512195/100‑1 (MQ=255)
                                 gTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                                                    <  2:372215/100‑1 (MQ=255)
                                  tAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                                                   >  1:1756862/1‑100 (MQ=255)
                                  tAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                                                   <  2:2911991/100‑1 (MQ=255)
                                   agagCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGa                                                                  <  1:2840523/100‑1 (MQ=255)
                                       cTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCg                                                              <  2:2689090/100‑1 (MQ=255)
                                         ggTATTTCATGAAGTGATGGGTGGCAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgc                                                            >  1:105171/1‑100 (MQ=255)
                                         ggTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgc                                                            >  2:1388287/1‑100 (MQ=255)
                                         ggTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgc                                                            >  2:1386315/1‑100 (MQ=255)
                                           tATTTCATGAAGTGATGGGTGTAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgcgc                                                          <  2:1094417/100‑1 (MQ=255)
                                              ttCATGAAGTGATGGGTGGAAACGATCATTGTCCTTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgag                                                       >  1:1210496/1‑100 (MQ=255)
                                              ttCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgag                                                       >  2:487558/1‑100 (MQ=255)
                                               tCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagc                                                      >  2:2103699/1‑100 (MQ=255)
                                                  tGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGcgagcgag                                                   <  2:2473225/100‑1 (MQ=255)
                                                   tccgatctGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAgg                                                  <  2:59675/93‑1 (MQ=255)
                                                   gAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAgg                                                  <  2:82484/100‑1 (MQ=255)
                                                   gAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAgg                                                  >  2:2298456/1‑100 (MQ=255)
                                                     aGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGt                                                >  1:1658921/1‑100 (MQ=255)
                                                      gTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTa                                               >  1:1111128/1‑100 (MQ=255)
                                                       tGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTATGATGTTGATTCGGCGCGAGCGAGGATa                                               >  1:2004087/1‑99 (MQ=255)
                                                        gATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaa                                             >  1:2060274/1‑100 (MQ=255)
                                                         aTGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaa                                            <  2:1821687/100‑1 (MQ=255)
                                                             gTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAatat                                        <  2:1544005/100‑1 (MQ=255)
                                                               tGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATc                                       <  2:2824314/100‑1 (MQ=255)
                                                                ggAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATc                                       >  2:1175295/1‑99 (MQ=255)
                                                                ggAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCg                                      >  2:2239154/1‑100 (MQ=255)
                                                                ggAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCg                                      >  2:2597603/1‑100 (MQ=255)
                                                                    aCGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaa                                  >  2:2786876/1‑100 (MQ=255)
                                                                     cGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaaa                                 >  1:1715004/1‑100 (MQ=255)
                                                                      gATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaaaa                                <  2:1668375/100‑1 (MQ=255)
                                                                       aTCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAg                               <  2:547494/100‑1 (MQ=255)
                                                                       aTCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAg                               >  1:663335/1‑100 (MQ=255)
                                                                        tCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGa                              >  2:2916933/1‑100 (MQ=255)
                                                                         cATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGAc                             <  1:1386310/100‑1 (MQ=255)
                                                                          aTTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACa                            <  2:2548068/100‑1 (MQ=255)
                                                                            ggggtacataCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGt                          >  2:701596/10‑100 (MQ=25)
                                                                              tCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTc                        <  1:789061/100‑1 (MQ=255)
                                                                              gttacataCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTc                        >  2:338508/8‑100 (MQ=25)
                                                                                aTTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCAc                      <  1:535672/100‑1 (MQ=255)
                                                                                aTTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCAc                      >  2:2890209/1‑100 (MQ=255)
                                                                                   ataCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCtt                   >  2:779315/3‑100 (MQ=38)
                                                                                   ataCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCtt                   >  2:1310070/3‑100 (MQ=38)
                                                                                    taCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCttt                  >  1:790851/2‑100 (MQ=38)
                                                                                    taCCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCttt                  >  2:296245/2‑100 (MQ=255)
                                                                                     aCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTa                 >  2:81918/1‑100 (MQ=38)
                                                                                     aCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTa                 >  2:3852/1‑100 (MQ=38)
                                                                                     aCCAGGCGTAAAGCTCTGCGCCTGGTATGTAAACCTAACTTAAGATGTTGATTCGGCGCGAGTGAGGGTAAAAATATCGTAAAAGGACAGTTCACCttt                  >  1:877412/1‑99 (MQ=38)
                                                                                     aCCAGGCGCAGTGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAAGCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCttt                  <  1:1615329/99‑1 (MQ=255)
                                                                                     aCCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTa                 <  2:212528/100‑1 (MQ=255)
                                                                                     aCCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTa                 <  1:1377260/100‑1 (MQ=255)
                                                                                      ccAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTAc                <  1:1034698/100‑1 (MQ=255)
                                                                                       cAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAACAATATCGTAAAAAGTCAGTTCACGTTTACg               >  1:2053055/1‑100 (MQ=255)
                                                                                       cAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACg               >  1:1660480/1‑100 (MQ=255)
                                                                                       cAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACg               >  1:2898621/1‑100 (MQ=255)
                                                                                        aGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACgg              >  2:2504580/1‑100 (MQ=255)
                                                                                         ggCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGa             >  2:2398806/1‑100 (MQ=255)
                                                                                         ggCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGa             <  2:1064095/100‑1 (MQ=255)
                                                                                           cgcAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACg           <  1:2607997/100‑1 (MQ=255)
                                                                                           cgcAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACg           <  2:610801/100‑1 (MQ=255)
                                                                                            gcAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGa          >  2:2772212/1‑100 (MQ=255)
                                                                                               gagCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCTAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGCACGATc        >  1:656015/1‑99 (MQ=255)
                                                                                                 acTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTGACCTTTACGGACGATCAtt     >  1:481447/2‑100 (MQ=255)
                                                                                                  cTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCAttt    >  1:2502268/1‑100 (MQ=255)
                                                                                                   tttACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCAtttt   >  2:2119877/1‑100 (MQ=255)
                                                                                                    |                                                                                                   
GATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTT  >  NC_000913/478658‑478856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: