Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 605,022 C→G 15.8% D135H (GAT→CAT)  nfsB ← dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913605,0220CG15.8% 301.7 / 45.7 113D135H (GAT→CAT) nfsBdihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive
Reads supporting (aligned to +/- strand):  ref base C (44/51);  new base G (8/10);  total (52/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

Only 100 of 121 total aligned reads displayed.

CAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTC  >  NC_000913/604923‑605121
                                                                                                   |                                                                                                   
cAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatc                                                                                                     >  1:1150091/1‑100 (MQ=255)
cAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTGGAGATAAACCTGTTTTGCCATCCACTCTGcatc                                                                                                     >  1:1102067/1‑100 (MQ=255)
  aaaCCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatcat                                                                                                   <  1:1899897/100‑1 (MQ=255)
    aCCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatcatca                                                                                                 <  1:962147/100‑1 (MQ=255)
      cTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCTAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATg                                                                                               >  2:213664/1‑100 (MQ=255)
       ttCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGc                                                                                              <  1:1648146/100‑1 (MQ=255)
       ttCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGc                                                                                              <  2:472355/100‑1 (MQ=255)
       ttCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGc                                                                                              >  1:995127/1‑100 (MQ=255)
        tCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCa                                                                                             <  2:1390987/100‑1 (MQ=255)
         cGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAg                                                                                            >  1:756841/1‑100 (MQ=255)
          gATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGa                                                                                           >  1:1646803/1‑100 (MQ=255)
           aTGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGAt                                                                                          <  1:1063706/100‑1 (MQ=255)
            tGGGTACCGGGTCCAGACCCAGAGCCGCCGCGCCGATCAGGAAGTGAGCGATGTTGGGATAAACCTGTTTTGCCAGCCACTCTGCATGATGATGCAGagt                                                                                         <  1:651527/100‑3 (MQ=255)
            tGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagt                                                                                         <  2:1928374/100‑3 (MQ=255)
            tGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagt                                                                                         <  2:238348/100‑3 (MQ=255)
            tGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagt                                                                                         <  2:1011353/100‑3 (MQ=255)
            tGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagt                                                                                         <  2:238399/100‑3 (MQ=255)
            tGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagt                                                                                         <  1:2875512/100‑3 (MQ=255)
            tGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagt                                                                                         <  1:2678929/100‑3 (MQ=255)
            tGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATc                                                                                         <  1:1194616/100‑1 (MQ=255)
            tGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATc                                                                                         <  2:1185621/100‑1 (MQ=255)
            tGGGTACCGCGTCAAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACATGTTTTGCCATCCACTCTGCACCATCATGCAGaac                                                                                         >  1:2438500/1‑98 (MQ=255)
             gggTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagtg                                                                                        <  1:2108340/100‑4 (MQ=255)
             gggTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCt                                                                                        <  1:149974/100‑1 (MQ=255)
              ngTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCtt                                                                                       >  2:2108585/2‑100 (MQ=255)
              ggTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagtgg                                                                                       <  2:2426573/100‑5 (MQ=255)
              ggTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCtt                                                                                       >  2:2057862/1‑100 (MQ=255)
                  ccGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACg                                                                                   >  1:903213/1‑100 (MQ=255)
                   ngcgTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACgg                                                                                  >  1:1345643/2‑100 (MQ=255)
                     cgTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTg                                                                                <  2:1365456/100‑1 (MQ=255)
                     cgTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTg                                                                                <  2:1365537/100‑1 (MQ=255)
                     cgTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTg                                                                                <  2:1150096/100‑1 (MQ=255)
                      gTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGc                                                                               <  2:2070242/100‑1 (MQ=255)
                      gTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCCGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGc                                                                               <  1:1950472/100‑1 (MQ=255)
                             cccAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCa                                                                         >  1:1878496/1‑99 (MQ=255)
                             cccAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAg                                                                        >  1:1292475/1‑100 (MQ=255)
                               cAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCg                                                                      >  1:1021910/1‑100 (MQ=255)
                                 gagCCGCCACCCCGAGCAGGAAGTTACCGACGTTGAGAGAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCgaa                                                                    <  1:2603087/100‑1 (MQ=255)
                                  agCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCgaag                                                                   <  1:2232832/100‑1 (MQ=255)
                                   gccgccACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCgaaga                                                                  >  2:359287/1‑100 (MQ=255)
                                    ccgccACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCgaagaa                                                                 >  2:1473025/1‑100 (MQ=255)
                                    ccgccACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCgaagaa                                                                 <  2:2676312/100‑1 (MQ=255)
                                        cACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTg                                                             <  1:1010097/100‑1 (MQ=255)
                                        cACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTg                                                             <  2:2343484/100‑1 (MQ=255)
                                         aCGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGc                                                            <  2:2593612/100‑1 (MQ=255)
                                          cGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCg                                                           <  2:2027929/100‑1 (MQ=255)
                                          cGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCg                                                           <  1:72430/100‑1 (MQ=255)
                                           gctgAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCg                                                           <  1:899749/96‑1 (MQ=255)
                                             cgtgCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGAcc                                                        <  1:692244/97‑1 (MQ=255)
                                             cGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGAcc                                                        >  1:2167576/1‑100 (MQ=255)
                                               aGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCtt                                                      <  1:2034409/100‑1 (MQ=255)
                                               aGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCAGCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCtt                                                      <  2:2632916/100‑1 (MQ=255)
                                                gCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCttt                                                     >  1:1372448/1‑100 (MQ=255)
                                                  aGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTAt                                                   <  2:1144144/100‑1 (MQ=255)
                                                   ggAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATc                                                  >  2:1584211/1‑100 (MQ=255)
                                                   ggAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATc                                                  >  2:1583808/1‑100 (MQ=255)
                                                     aaGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGt                                                <  2:2751607/100‑1 (MQ=255)
                                                     aaGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGt                                                >  1:1897887/1‑100 (MQ=255)
                                                     aaGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGt                                                >  1:2252159/1‑100 (MQ=255)
                                                     aaGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGt                                                <  2:145288/100‑1 (MQ=255)
                                                         tACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTcgc                                            <  1:407640/100‑1 (MQ=255)
                                                          aCCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTcgcg                                           <  2:1015204/100‑1 (MQ=255)
                                                          aCCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTcgcg                                           <  1:1159811/100‑1 (MQ=255)
                                                             gACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCt                                        >  1:1379609/1‑100 (MQ=255)
                                                             gACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCt                                        >  1:1322384/1‑100 (MQ=255)
                                                              aCGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCtt                                       >  1:2172204/1‑100 (MQ=255)
                                                                gTTTAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTc                                     <  1:883224/100‑1 (MQ=255)
                                                                gTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTc                                     >  2:2097576/1‑100 (MQ=255)
                                                                gTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTc                                     <  2:394577/100‑1 (MQ=255)
                                                                   gagaTAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCt                                  >  2:64170/1‑100 (MQ=255)
                                                                   gagaTAAACCTGTTGTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCt                                  <  1:120317/100‑1 (MQ=255)
                                                                    agaTAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTc                                     >  1:958842/1‑96 (MQ=255)
                                                                    agaTAAACCTCTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTCTATCGTTCGCGGCTTTCGCtt                                 >  1:110097/1‑100 (MQ=255)
                                                                       tcaaCCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTCCGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTcc                               <  2:860251/97‑1 (MQ=255)
                                                                       tAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCg                              <  2:315694/100‑1 (MQ=255)
                                                                       tAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCg                              <  2:1255937/100‑1 (MQ=255)
                                                                       tAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCg                              >  1:2150584/1‑100 (MQ=255)
                                                                       tAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCg                              >  1:85389/1‑100 (MQ=255)
                                                                          aCCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCACCGAAGAACTTGCAACCTTTATAGTTCGCGGCTTTCGCTTc                                >  1:2454210/1‑95 (MQ=255)
                                                                              gTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGc                       <  1:2570202/100‑1 (MQ=255)
                                                                                 ttGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCaaa                    >  1:1469496/1‑100 (MQ=255)
                                                                                 ttGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTACCGTTCTCGCCTTTCGCTTCCGGCTTGGCaaa                    >  1:2060060/1‑100 (MQ=255)
                                                                                  tGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAg                   >  2:1955835/1‑100 (MQ=255)
                                                                                  accgtaaagatctGCATGATGATGCAGATCTTTAGGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAg                   >  2:2629938/11‑100 (MQ=37)
                                                                                   gCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGc                  <  1:2029310/100‑1 (MQ=255)
                                                                                     gtaaagatctGCATGATGATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCgg                >  2:539809/8‑100 (MQ=255)
                                                                                     gtaaagatctGCATGATGATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCgg                >  2:2104509/8‑100 (MQ=255)
                                                                                     cATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCgg                <  1:896905/100‑1 (MQ=255)
                                                                                      aTCCACTCTGCATCATCATGCAGATCTTTTCGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGc               <  2:1102072/100‑1 (MQ=255)
                                                                                      aTCCACTCTGCATCATCATGCACATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGc               >  1:1688116/1‑100 (MQ=255)
                                                                                       aaagatctGCATGATGATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGcc              >  1:2021088/6‑100 (MQ=255)
                                                                                        ccACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCa             <  1:1233553/100‑1 (MQ=255)
                                                                                          gatctGCATGATGATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATc           >  2:2244527/3‑100 (MQ=255)
                                                                                          gatctGCATGATGATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATc           >  2:1336784/3‑100 (MQ=255)
                                                                                           ctctGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCg          >  2:843001/1‑100 (MQ=255)
                                                                                              tGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCa       >  1:63513/1‑100 (MQ=255)
                                                                                               gCATGATGATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGACCATCGGCAt      >  1:2326215/1‑100 (MQ=255)
                                                                                               gCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCAt      >  2:473613/1‑100 (MQ=255)
                                                                                                catcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATc     <  1:359286/100‑1 (MQ=255)
                                                                                                   catcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTc  <  2:1546031/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTC  >  NC_000913/604923‑605121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: