Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 654,563 C→G 16.2% intergenic (+21/+20) citB → / ← dcuC response regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913654,5630CG16.2% 365.4 / 62.5 136intergenic (+21/+20)citB/dcuCresponse regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base C (53/61);  new base G (10/12);  total (63/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01

Only 100 of 138 total aligned reads displayed.

CGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCA  >  NC_000913/654464‑654662
                                                                                                   |                                                                                                   
cgcCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAAc                                                                                                     <  2:1371685/100‑1 (MQ=255)
 gcCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACt                                                                                                    >  1:356056/1‑100 (MQ=255)
   cAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTg                                                                                                  <  2:105215/100‑1 (MQ=255)
   cAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTg                                                                                                  <  2:645136/100‑1 (MQ=255)
     gccgccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCCCAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTcc                                                                                                <  2:1301986/100‑1 (MQ=255)
     gccgccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGcc                                                                                                <  2:280874/100‑1 (MQ=255)
         ccATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCg                                                                                            >  2:2508514/1‑100 (MQ=255)
           aTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCgg                                                                                           <  1:2902230/99‑1 (MQ=255)
           aTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGc                                                                                          <  2:75840/100‑1 (MQ=255)
           aTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGc                                                                                          <  1:2775493/100‑1 (MQ=255)
           aTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGc                                                                                          <  1:2246497/100‑1 (MQ=255)
           aTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGc                                                                                          <  2:1929586/100‑1 (MQ=255)
           aTCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGc                                                                                          <  2:1686760/100‑1 (MQ=255)
            tCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCa                                                                                         <  2:1589465/100‑1 (MQ=255)
             cTGATTATTGCTGAAATTGTTCACGGCAAAGTGGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGct                                                                                         <  2:869348/99‑2 (MQ=38)
              tGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGCttt                                                                                       <  2:246598/100‑1 (MQ=38)
              tGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCGGCttt                                                                                       <  2:246317/100‑1 (MQ=38)
              tGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCAtt                                                                                       >  2:447694/1‑100 (MQ=255)
               gATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTa                                                                                      <  1:2146407/100‑1 (MQ=255)
                attattGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAATGCCGGAGGCAAGTTTCCTCCGGCTTTat                                                                                     <  2:1660722/100‑2 (MQ=37)
                attattGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTAc                                                                                     <  2:648104/100‑1 (MQ=255)
                attattGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTAc                                                                                     >  1:648101/1‑100 (MQ=255)
                  tattGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTa                                                                                   <  1:2214516/100‑1 (MQ=255)
                   attGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAATGCCGGAGGCAAGTTTCCTCCGGCTTTatcat                                                                                  <  1:1265840/100‑5 (MQ=37)
                    ttGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAtt                                                                                 >  2:551389/1‑100 (MQ=255)
                     tGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAttt                                                                                <  2:356057/100‑1 (MQ=255)
                     tGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAttt                                                                                <  1:1227949/100‑1 (MQ=255)
                     tGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAttt                                                                                >  1:651208/1‑100 (MQ=255)
                     tGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAttt                                                                                <  2:19021/100‑1 (MQ=255)
                      gCTGAAATTGTTCACGGCAAAGTTGGCAGACCAGAACGCATATACCACAGTGGGTGACATGATAAAGCCGAAGGAAACTTGCCTCCGGCATTACTATTTa                                                                               >  1:1753855/1‑100 (MQ=255)
                       cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGTCATTACTATTTAc                                                                              >  2:2241177/1‑100 (MQ=255)
                         naaaTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACtt                                                                            >  1:594712/2‑100 (MQ=255)
                         gAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACtt                                                                            >  2:1744174/1‑100 (MQ=255)
                          aaaTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTg                                                                           <  1:2166605/100‑1 (MQ=255)
                                ttCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCtgtg                                                                     >  2:203979/1‑100 (MQ=255)
                                ttCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCtgtg                                                                     <  1:2631825/100‑1 (MQ=255)
                                  cACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGAc                                                                   >  2:1492749/1‑100 (MQ=255)
                                  cACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGAc                                                                   <  1:551386/100‑1 (MQ=255)
                                   aCGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGAcc                                                                  >  2:2123080/1‑100 (MQ=255)
                                     ggCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgc                                                                <  2:651211/100‑1 (MQ=255)
                                     ggCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgc                                                                >  2:1276400/1‑100 (MQ=255)
                                      gCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgct                                                               >  2:2032780/1‑100 (MQ=255)
                                         aaGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctgct                                                            >  2:1168483/1‑100 (MQ=255)
                                           gTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCtgctgc                                                          <  1:1669604/100‑1 (MQ=255)
                                            ttGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGcc                                                         <  1:869409/100‑1 (MQ=255)
                                            ttGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGcc                                                         <  2:48233/100‑1 (MQ=255)
                                            ttGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGcc                                                         <  1:2742408/100‑1 (MQ=255)
                                               gCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGtt                                                      <  1:1360432/100‑1 (MQ=255)
                                               gCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGtt                                                      >  2:2472254/1‑100 (MQ=255)
                                               gCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGtt                                                      <  2:1585806/100‑1 (MQ=255)
                                                 aGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGGTcc                                                    >  2:2557250/1‑100 (MQ=255)
                                                   aCCACAACGCATATACCACAGTGGGTGTCATGATTACGCCGGAGGAAACTTGCCTCCGGCATTACTATTTCCTTGCCTGTCACCGCTGCTGCCGTTc                                                     >  1:675297/1‑97 (MQ=255)
                                                   aCCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTg                                                  <  2:2586434/100‑1 (MQ=255)
                                                    ccACAACACACATACCACAGTGGGTGACATGAGAAAACCCGAGGAAACTTGCCCCCGGCATTACCATTTACTTGCCTGTGACCGCTGCTGCCGTACCTgg                                                 <  1:168424/100‑1 (MQ=255)
                                                     cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGc                                                <  1:2675010/100‑1 (MQ=255)
                                                     cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGc                                                <  1:1597446/100‑1 (MQ=255)
                                                     cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGc                                                <  1:1042649/100‑1 (MQ=255)
                                                      acaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCa                                               <  2:1702189/100‑1 (MQ=255)
                                                        aaCGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAcc                                             >  2:2891709/1‑100 (MQ=255)
                                                         aCGCATATACCACATTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAcc                                             <  1:2901944/99‑1 (MQ=255)
                                                         aCGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCa                                            <  2:2270259/100‑1 (MQ=255)
                                                          cGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTGCGGCATTACTATTTACTTGCCTGTAACCGCTGCTGCCGTTCCTGGCACCAt                                           >  1:385313/1‑100 (MQ=255)
                                                          cGCATATACCACAGTGGGGGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCAt                                           >  1:2065067/1‑100 (MQ=255)
                                                           gCATATACCACAGTGGGTGACATGATAAAGCCGGCGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATc                                          <  1:1536268/100‑1 (MQ=255)
                                                            natatACCACAGTGGGTGCCATGATAAAGCCGCAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCa                                            >  1:1914487/2‑97 (MQ=255)
                                                             atatACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAg                                        <  1:395316/100‑1 (MQ=255)
                                                               ataCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGct                                      >  2:1885985/1‑100 (MQ=255)
                                                                taCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctc                                     >  2:1268963/1‑100 (MQ=255)
                                                                taCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctc                                     >  1:2100061/1‑100 (MQ=255)
                                                                 aCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctct                                    <  1:2820503/100‑1 (MQ=255)
                                                                  ccACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTg                                   <  1:263170/100‑1 (MQ=255)
                                                                    acaGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTa                                 <  2:1569518/100‑1 (MQ=255)
                                                                      aGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGc                               >  1:738046/1‑100 (MQ=255)
                                                                        tGGGTGACATGATAAAGCCGGAGGAAACGTGCCTGCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCaa                             <  1:2819606/100‑1 (MQ=255)
                                                                          ggTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTTCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACg                           <  1:1102637/100‑1 (MQ=255)
                                                                               cATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTAGTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAAc                      <  1:1858630/100‑1 (MQ=255)
                                                                               cATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATCAc                      >  1:959450/1‑100 (MQ=255)
                                                                               cATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAAc                      <  1:2692644/100‑1 (MQ=255)
                                                                                   gtAATGCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATc                  >  1:2225713/2‑100 (MQ=38)
                                                                                   aTAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAGCATCAGGTCTGTAGCAACGATAACAAt                   >  1:2430773/1‑99 (MQ=255)
                                                                                    tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCa                 >  1:2333974/1‑100 (MQ=255)
                                                                                     aaaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCac                >  2:2138686/1‑100 (MQ=255)
                                                                                      atgCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacc               >  2:2202814/3‑100 (MQ=255)
                                                                                      aaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacc               <  2:1839813/100‑1 (MQ=255)
                                                                                      aaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacc               <  2:2260560/100‑1 (MQ=255)
                                                                                      aaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCAACATCAGCTCTGTAGCAACGATAACAATCacc               >  2:1417500/1‑100 (MQ=255)
                                                                                       tgCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacca              >  1:204269/2‑100 (MQ=255)
                                                                                       tgCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACATGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacc               >  2:2055652/2‑99 (MQ=38)
                                                                                       aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacca              >  1:528204/1‑100 (MQ=255)
                                                                                        gCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaa             >  2:1173643/1‑100 (MQ=255)
                                                                                        gCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaa             >  2:262547/1‑100 (MQ=255)
                                                                                        gCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaa             >  2:1405022/1‑100 (MQ=255)
                                                                                           ggaggcAAGTTTCCTCCGGCTTTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaacc          >  2:539881/1‑100 (MQ=255)
                                                                                           ggaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaacc          >  2:1078370/1‑100 (MQ=255)
                                                                                           ggaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaacc          <  1:1097321/100‑1 (MQ=255)
                                                                                           gaaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaacc          >  2:1976823/3‑100 (MQ=255)
                                                                                              ggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACcaac       >  2:2818307/1‑100 (MQ=255)
                                                                                               gaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACcaaca      <  2:2520321/100‑1 (MQ=255)
                                                                                                aaaCTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACcaacaa     >  1:2023006/1‑100 (MQ=255)
                                                                                                  aCTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGc   <  2:2241976/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCA  >  NC_000913/654464‑654662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: