Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 87,884 T→A 17.4% intergenic (+36/‑144) ilvH → / → cra acetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091387,8840TA17.4% 280.4 / 51.9 121intergenic (+36/‑144)ilvH/craacetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand):  ref base T (43/56);  new base A (13/8);  total (56/65)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.05e-01

Only 100 of 122 total aligned reads displayed.

TTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATAC  >  NC_000913/87787‑87983
                                                                                                 |                                                                                                   
ttGTGGAGGTTGGTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTc                                                                                                    <  1:284686/99‑1 (MQ=255)
  gtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACg                                                                                                 >  1:857134/1‑100 (MQ=255)
    ggAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAAAGCGCAATTTACAGCCCAACATGTCACGtt                                                                                               >  2:712803/1‑100 (MQ=255)
     gAGGTTGCTCGCTCTGGTGTGGACGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTg                                                                                              >  1:1690757/1‑100 (MQ=255)
       ggTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg                                                                                             >  1:695306/1‑99 (MQ=255)
        gTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGTCATGTTGGGc                                                                                           <  2:510193/100‑1 (MQ=255)
        gTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGc                                                                                           <  2:86166/100‑1 (MQ=255)
        gTTGCTCGCTCTGGGGTGGTCGGACTTTCTCGCGGCGATAAAATTATGCGTTGGGAATTATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGc                                                                                           <  1:1594085/100‑1 (MQ=255)
         ttGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCt                                                                                          <  1:1235961/100‑1 (MQ=255)
          tGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                         <  2:312840/100‑2 (MQ=255)
          tGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                         <  1:1877989/100‑2 (MQ=255)
          tGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtt                                                                                         >  2:2135910/1‑100 (MQ=255)
            ctcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAACGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtgt                                                                                        <  1:2429127/99‑3 (MQ=38)
            ctcgctcTGGTGTGGTCGGACTGTCGCGCGGCGATAAAATACTGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTTGGCtttt                                                                                       >  1:2343176/1‑100 (MQ=255)
            ctcgctcTGGTGTGCTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttt                                                                                       <  1:1987603/100‑1 (MQ=255)
              cgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttttt                                                                                     >  2:339474/1‑100 (MQ=255)
               gctcTGGTGTGGTCGAACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtgtaaat                                                                                    <  2:2869870/100‑7 (MQ=37)
                ctctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTg                                                                                   <  1:1116008/100‑1 (MQ=255)
                  ctGGTGTGGTCGTACTTTCGCGCGGAGATAAAATAATGCGTTGTGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACACGTTGGGCtgtaaat                                                                                    <  1:1730902/97‑7 (MQ=37)
                   tGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                 <  1:2616969/99‑1 (MQ=255)
                   tGGGGTGGTCGGACTTTCGCGCGGCGATAAAATAAGGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                 <  1:979750/99‑1 (MQ=255)
                    ggTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa                                                                               <  1:2085628/100‑1 (MQ=255)
                    ggTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTGGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa                                                                               <  1:1243031/100‑1 (MQ=255)
                    ggTGTGGTCGGACTTTAGCGCGGCGATAAAATAATGCGTTGAGAATGATGTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa                                                                               >  1:1399233/1‑100 (MQ=255)
                       gtgGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCttttttttcttatt                                                                             >  1:1851260/1‑92 (MQ=255)
                       gtgGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc                                                                            <  2:195388/100‑1 (MQ=255)
                         ggTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttttttttttttt                                                                             >  1:597667/1‑90 (MQ=255)
                         ggTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAg                                                                          >  2:1572990/1‑100 (MQ=255)
                           tCGGACTTTCGCGCTGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                        <  1:1702178/100‑1 (MQ=255)
                           tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                        >  2:1033369/1‑100 (MQ=255)
                           tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                        <  2:1007414/100‑1 (MQ=255)
                           tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                        <  2:2566135/100‑1 (MQ=255)
                           tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                        <  1:2491890/100‑1 (MQ=255)
                           tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                        >  2:2883774/1‑100 (MQ=255)
                           tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                        <  2:310276/100‑1 (MQ=255)
                            cGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTgg                                                                       <  2:912373/100‑1 (MQ=255)
                             ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTggg                                                                      <  1:539764/100‑1 (MQ=255)
                                cTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAAc                                                                   >  2:954837/1‑100 (MQ=255)
                                 tttCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAAcc                                                                  <  2:1783790/100‑1 (MQ=255)
                                    cgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTgg                                                               <  1:2791951/100‑1 (MQ=255)
                                    cgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTgg                                                               >  2:2050698/1‑100 (MQ=255)
                                    cgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTgg                                                               >  2:1897296/1‑100 (MQ=255)
                                     gcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTgg                                                               <  2:1492324/99‑1 (MQ=255)
                                      cgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGaa                                                             <  2:981950/100‑1 (MQ=255)
                                           cgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaaaa                                                        <  2:778842/100‑1 (MQ=255)
                                            gATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg                                                       <  2:2474459/100‑1 (MQ=255)
                                            gATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg                                                       >  1:2742984/1‑100 (MQ=255)
                                            gATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg                                                       <  2:1666211/100‑1 (MQ=255)
                                             aTAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGc                                                      <  1:889363/100‑1 (MQ=255)
                                             aTAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGc                                                      >  1:923558/1‑100 (MQ=255)
                                               aaaaTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAg                                                    >  2:751203/1‑100 (MQ=255)
                                               aaaaTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAg                                                    <  2:1242010/100‑1 (MQ=255)
                                                aaaTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGt                                                   >  1:154025/1‑100 (MQ=255)
                                                      tGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGc                                             >  2:2791810/1‑100 (MQ=255)
                                                       gCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCg                                              <  1:2036370/98‑1 (MQ=255)
                                                       gCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCa                                            <  2:951955/100‑1 (MQ=255)
                                                       gCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCa                                            >  2:857601/1‑100 (MQ=255)
                                                        cGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAg                                           >  2:1453474/1‑100 (MQ=255)
                                                        cGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAg                                           >  2:1997162/1‑100 (MQ=255)
                                                         gTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGt                                          >  2:763764/1‑100 (MQ=255)
                                                              gaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGCAATAAAAGCAGTTGCCGCAGTTAAtt                                     >  1:2416876/1‑100 (MQ=255)
                                                                aTGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAAtttt                                   <  2:1571436/100‑1 (MQ=255)
                                                                 tGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTc                                  <  2:833517/100‑1 (MQ=255)
                                                                  gATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCt                                 >  1:662749/1‑100 (MQ=255)
                                                                  gATCTCAATGCGCAATTTACAGCCCAACATGTCAAGTTGGGCTTTTTTTGCGAAATCAGTGGGAACATGGAATAAAAGCAGTTGCCGCAGTTAATTTTCt                                 >  2:1675176/1‑100 (MQ=255)
                                                                    tctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgc                               <  1:1151849/100‑1 (MQ=255)
                                                                    tctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgc                               >  2:623892/1‑100 (MQ=255)
                                                                       cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTTTTTAATCAGTGAGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTTCGCt                            >  1:2217911/1‑100 (MQ=255)
                                                                       cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCt                            <  1:1638549/100‑1 (MQ=255)
                                                                        aaTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCt                            >  1:2692519/1‑99 (MQ=255)
                                                                         aTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCtt                           <  1:662970/99‑1 (MQ=255)
                                                                           gcgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGa                        <  1:2883755/100‑1 (MQ=255)
                                                                           gcgcAATTTACAGCCAAACATGGCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTGAATTTTCTGCGCTTAg                         <  1:696925/99‑1 (MQ=255)
                                                                            cgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGAt                       <  1:2135897/100‑1 (MQ=255)
                                                                              gcaaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGt                     >  2:321465/9‑100 (MQ=37)
                                                                              gcaaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCAAAATCAGTGGGAACCAGGCATAAAAACAGTTACCGCAGGTAAGTTTCAGCGCTTAGa                        >  1:2411184/9‑97 (MQ=16)
                                                                               caaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGCTGtt                    >  2:1538577/8‑100 (MQ=37)
                                                                               caaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                    >  2:2401847/8‑100 (MQ=37)
                                                                               caaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                    >  1:2208640/8‑100 (MQ=38)
                                                                               caaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                    >  2:1918860/8‑100 (MQ=37)
                                                                               aaTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCTCAGTTAATTTTCTGCGCTTAGATGtt                    <  2:597670/100‑1 (MQ=255)
                                                                               aaTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                    >  2:1008786/1‑100 (MQ=255)
                                                                               aaTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                    <  1:1997151/100‑1 (MQ=255)
                                                                                aaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTa                   >  2:1702909/7‑100 (MQ=38)
                                                                                  ttACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGGTGTTAAt                 <  2:1115489/100‑1 (MQ=255)
                                                                                    aaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGa               >  1:2101957/3‑100 (MQ=255)
                                                                                    aCAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGAAGTTTATTTTCGGCGCTTAGATGTTAGTGa               >  1:1638400/1‑100 (MQ=255)
                                                                                     cAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGaa              <  2:1590271/100‑1 (MQ=255)
                                                                                     aaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGACGCAGTTAATTTTCTGCGCTTAGATGTTAATGaa              >  2:822463/2‑100 (MQ=255)
                                                                                      ngCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAt             >  2:1533219/2‑100 (MQ=255)
                                                                                      aGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAt             >  2:1261552/1‑100 (MQ=255)
                                                                                       gCCCAACGTGACATGTTGGGCTTTTTTTTTTAATTCATTGGGAACCTGGAATAAAAGCAGTTGCCTCAGTTAATTTTCTGCGCTTAGATGTTAATGAAtt            >  1:2892328/1‑100 (MQ=255)
                                                                                       gCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAtt            <  2:2217707/100‑1 (MQ=255)
                                                                                         ccAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTagatcggaag          >  1:1604447/1‑91 (MQ=255)
                                                                                           aaCATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAACAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAc        <  2:57952/100‑1 (MQ=255)
                                                                                           aaCATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAc        <  2:377390/100‑1 (MQ=255)
                                                                                             cATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAccc      <  2:2035430/100‑1 (MQ=255)
                                                                                               tGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCAt    <  2:1478256/100‑1 (MQ=255)
                                                                                                gTCACGTTGGGCTTTTTTTTCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATa   >  1:918317/1‑100 (MQ=255)
                                                                                                 tCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATAc  >  2:852547/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
TTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATAC  >  NC_000913/87787‑87983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: