Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 844,513 T→A 44.1% Q323L (CAG→CTG)  ybiO ← mechanosensitive channel protein, intermediate conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913844,5130TA44.1% 33.2 / 148.8 109Q323L (CAG→CTG) ybiOmechanosensitive channel protein, intermediate conductance
Reads supporting (aligned to +/- strand):  ref base T (21/40);  new base A (23/25);  total (44/65)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-01

Only 100 of 111 total aligned reads displayed.

ATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACA  >  NC_000913/844414‑844608
                                                                                                   |                                                                                               
aTAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCt                                                                                                 >  1:436149/1‑100 (MQ=255)
 tAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTg                                                                                                <  1:1344300/100‑1 (MQ=255)
 tAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTg                                                                                                <  1:1471290/100‑1 (MQ=255)
  aaaaTAGGGGCGTGCCAGACAGTGCCACACCAGCGCAAAGGCGCGGATAAGCAGGCTGAGACAGGCCAGCGAATGCGCCGCGAGGTTGAGCAAatgatg                                                                                                >  1:2707334/1‑99 (MQ=255)
  aaaaTAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAgg                                                                                               >  2:363940/1‑100 (MQ=255)
   aaaTAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAggg                                                                                              <  2:708507/100‑1 (MQ=255)
        ggCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAAtc                                                                                         >  2:1736981/1‑100 (MQ=255)
        ggCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAAtc                                                                                         <  2:2886983/100‑1 (MQ=255)
           gcTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATctct                                                                                      >  1:2335483/1‑100 (MQ=255)
            cTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCtt                                                                                     <  1:1100387/100‑1 (MQ=255)
             ttGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCttt                                                                                    >  1:2107358/1‑100 (MQ=255)
              tGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCtttt                                                                                   <  2:2692259/100‑1 (MQ=255)
              tGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCtttt                                                                                   <  1:833500/100‑1 (MQ=255)
               gcgagccagTGCCACACCAGCGCAAAGGCGCGGATAAACGGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGCGCCAGTGCTGGGTAAGATCttttt                                                                                  >  1:2148808/4‑100 (MQ=255)
               gccagccaGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCttttt                                                                                  <  1:1164926/100‑1 (MQ=255)
               gccagccaGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCttttt                                                                                  >  1:2106539/1‑100 (MQ=255)
               gccagccaGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCttttt                                                                                  <  1:2758604/100‑1 (MQ=255)
               gccagccaGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCttttt                                                                                  <  2:527583/100‑1 (MQ=255)
               gccagccaGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCttttt                                                                                  <  2:1161183/100‑1 (MQ=255)
               gccagccaGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCttttt                                                                                  >  2:1390655/1‑100 (MQ=255)
                ccagccagTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCtttttt                                                                                 <  1:56211/100‑1 (MQ=255)
                ccagccagTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCttttt                                                                                  >  2:1345278/1‑99 (MQ=255)
                 cagccagTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTtta                                                                                >  2:2435662/1‑100 (MQ=255)
                  agccagTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTttat                                                                               <  1:2383045/100‑1 (MQ=255)
                  agccagTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTttat                                                                               <  1:2383001/100‑1 (MQ=255)
                   gccagTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTttatt                                                                              <  1:2261245/100‑1 (MQ=255)
                    ccagTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTttatta                                                                             <  2:1198211/100‑1 (MQ=255)
                     cagTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTAc                                                                            <  1:759841/100‑1 (MQ=255)
                     cagTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTAc                                                                            <  2:2379558/100‑1 (MQ=255)
                     cagTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTttat                                                                               <  1:2408082/97‑1 (MQ=255)
                      agTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTAc                                                                            >  1:115213/1‑99 (MQ=255)
                      agTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACg                                                                           >  1:115212/1‑100 (MQ=255)
                       gTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGa                                                                          >  1:2028848/1‑100 (MQ=255)
                       gTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGa                                                                          >  2:1544058/1‑100 (MQ=255)
                        tGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGaa                                                                         <  2:409215/100‑1 (MQ=255)
                        tGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGa                                                                          >  1:1342313/1‑99 (MQ=255)
                         gCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGaaa                                                                        <  1:2144622/100‑1 (MQ=255)
                         gCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGaaa                                                                        >  1:2786868/1‑100 (MQ=255)
                         gCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGaa                                                                         >  1:1358402/1‑99 (MQ=255)
                           cacaCCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGa                                                                      >  2:713694/1‑100 (MQ=255)
                             cacCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATc                                                                    >  1:2063719/1‑100 (MQ=255)
                             cacCAACGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATTCTGGGTAATCTCTTTTTTATTACGAAAGATc                                                                    <  1:2480188/100‑1 (MQ=255)
                              acCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCa                                                                   <  1:879841/100‑1 (MQ=255)
                              acCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCa                                                                   <  1:2850609/100‑1 (MQ=255)
                               ccAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAg                                                                  >  2:1946960/1‑100 (MQ=255)
                               ccAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAg                                                                  >  2:265886/1‑100 (MQ=255)
                                cAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAgg                                                                 <  2:1822084/100‑1 (MQ=255)
                                 aGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGt                                                                <  2:205685/100‑1 (MQ=255)
                                 aGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGt                                                                <  2:818770/100‑1 (MQ=255)
                                 aGCGCAAAGGCGCGGATAAACAGGCGGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGt                                                                <  1:1829126/100‑1 (MQ=255)
                                  gcgcAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTa                                                               <  2:1352465/100‑1 (MQ=255)
                                   cgcAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTAc                                                              <  2:1556506/100‑1 (MQ=255)
                                    gcAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACa                                                             <  2:1889425/100‑1 (MQ=255)
                                    gcAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACa                                                             <  2:1889349/100‑1 (MQ=255)
                                       aaGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACg                                                          <  1:2664595/100‑1 (MQ=255)
                                         ggCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGcc                                                        >  2:381812/1‑100 (MQ=255)
                                          gcgcGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGccc                                                       >  2:2285156/1‑100 (MQ=255)
                                           cgcgGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCa                                                      <  1:1946951/100‑1 (MQ=255)
                                           cgcgGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCa                                                      <  1:1542692/100‑1 (MQ=255)
                                           cgcgGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCa                                                      >  2:231440/1‑100 (MQ=255)
                                           cgcgGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCa                                                      <  1:1746533/100‑1 (MQ=255)
                                           cgcgGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCa                                                      >  1:209610/1‑100 (MQ=255)
                                            gccgATAAACAGGCTGAAAAAGTCCAGCCAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACg                                                          <  1:2664576/92‑1 (MQ=255)
                                                aTAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACgg                                                 <  2:801687/100‑1 (MQ=255)
                                                    aCAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCAt                                             <  1:1862531/100‑1 (MQ=255)
                                                     cAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCCGACGGTCAt                                             >  1:1293962/1‑99 (MQ=255)
                                                     cAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATg                                            <  2:879401/100‑1 (MQ=255)
                                                      aGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGc                                           <  2:9126/100‑1 (MQ=255)
                                                      aGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGc                                           <  1:1625676/100‑1 (MQ=255)
                                                         cagAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGcaca                                        >  2:1977517/3‑100 (MQ=255)
                                                         cTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTGTTATTACGCCGGATAAGGCACAACGCGCAGACGGTCATGcaca                                        >  1:292995/1‑100 (MQ=255)
                                                         cTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACGACGCCCAGACGGTCATGcaca                                        <  2:2301310/100‑1 (MQ=255)
                                                          tGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAg                                       >  2:898985/1‑100 (MQ=255)
                                                          tGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAg                                       <  1:1952257/100‑1 (MQ=255)
                                                            aaaaaGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAg                                       <  1:1773315/98‑1 (MQ=255)
                                                                  gCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAatga                               <  2:831312/100‑1 (MQ=255)
                                                                  gCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAatga                               <  2:2019642/100‑1 (MQ=255)
                                                                  gCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAatga                               <  1:698062/100‑1 (MQ=255)
                                                                     aGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCAAAGCATAatgat                              >  1:478320/1‑98 (MQ=255)
                                                                      gCGAATGCTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGAc                           <  1:914268/100‑1 (MQ=255)
                                                                        gAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGt                         >  2:479073/1‑100 (MQ=255)
                                                                           tGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCg                      <  1:1282910/100‑1 (MQ=255)
                                                                           tGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCg                      <  2:477189/100‑1 (MQ=255)
                                                                             cTCCGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGcc                    >  1:2310179/1‑100 (MQ=255)
                                                                              tCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCa                   >  1:76808/1‑100 (MQ=255)
                                                                               ccGCGAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAg                  >  2:2598591/1‑100 (MQ=255)
                                                                                  cgAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAgcgc               <  2:2070430/100‑1 (MQ=255)
                                                                                  cgAAGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCcgcg                >  2:1435994/1‑99 (MQ=255)
                                                                                     aGTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCAcc            <  2:801168/100‑1 (MQ=255)
                                                                                      gTTGAGCAAATGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCt           >  2:1451090/1‑100 (MQ=255)
                                                                                       ttGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTa          <  2:451191/100‑1 (MQ=255)
                                                                                         gAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGGCGTTCGCCAGCGCACCTATc        <  1:1779757/100‑1 (MQ=255)
                                                                                          aGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCt       >  2:507646/1‑100 (MQ=255)
                                                                                           gCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTg      <  2:48769/100‑1 (MQ=255)
                                                                                           gCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGACCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCt       >  1:2095761/1‑99 (MQ=255)
                                                                                             aaaTGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTa    <  1:229570/100‑1 (MQ=255)
                                                                                              aaTGCAGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTAc   >  1:1357095/1‑100 (MQ=255)
                                                                                              aaTGCAGCGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTAc   <  1:2657834/100‑1 (MQ=255)
                                                                                               aTGCTTGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACa  <  1:1437628/100‑1 (MQ=255)
                                                                                               aTGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACa  <  1:1644670/100‑1 (MQ=255)
                                                                                                   |                                                                                               
ATAAAATAGGCGCTTGCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACA  >  NC_000913/844414‑844608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: