Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 940,751 T→C 15.5% intergenic (+31/‑208) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913940,7510TC15.5% 260.1 / 40.4 117intergenic (+31/‑208)serS/dmsAseryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (52/46);  new base C (6/12);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

Only 100 of 118 total aligned reads displayed.

TGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAT  >  NC_000913/940654‑940849
                                                                                                 |                                                                                                  
tGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACACAATTTTTCTGAATCTAAAAAGCGCCt                                                                                                    >  1:2110505/1‑98 (MQ=255)
 gATGGTGGTATTGAAGTACCAGAAGGTCTGCGTCTGTATATCAACGAAATGGAAGATATTGGCTAATACCCAATTTTTCTGAATCTAAAAGGCGCCTGCg                                                                                                 <  1:11852/100‑1 (MQ=255)
 gATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCg                                                                                                 <  2:1124630/100‑1 (MQ=255)
 gATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCg                                                                                                 <  2:285900/100‑1 (MQ=255)
   tGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCggg                                                                                               >  2:1547531/1‑100 (MQ=255)
      tCGTATTGACGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGgcgc                                                                                            <  2:1132683/100‑1 (MQ=255)
           ttGAAGTACCATAAGTTCTGCGTCCGTTTATGTTCGGACTGTAATATAGTGGCTAATACCCAATTTTTCTGAATCTAATAAGCGCCCGCAGGCGCttttt                                                                                       <  1:2664387/100‑1 (MQ=255)
            tGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCttttta                                                                                      <  2:916632/100‑2 (MQ=255)
            tGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCttttta                                                                                      <  2:2204770/100‑2 (MQ=255)
            tGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCttttta                                                                                      <  2:2838472/100‑2 (MQ=255)
             gAAGTACCAGAAGTTCTTCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGATTCTATGAATCGCCTGCGGTCTCttttttt                                                                                     <  1:142321/100‑1 (MQ=255)
             gAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCttttttt                                                                                     >  2:923623/1‑100 (MQ=255)
             gAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCttttttt                                                                                     >  1:2169043/1‑100 (MQ=255)
             gAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttag                                                                                     <  2:2039827/100‑3 (MQ=255)
              aaGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTg                                                                                    >  2:351937/1‑100 (MQ=255)
              aaGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTg                                                                                    <  1:510923/100‑1 (MQ=255)
               aGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTg                                                                                    <  1:2492825/99‑1 (MQ=255)
               aGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGt                                                                                   <  2:2728720/100‑1 (MQ=255)
                gTACCAGAAGTTCTGCGTCCGTATATGCACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtc                                                                                  <  1:2613250/100‑1 (MQ=255)
                gTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagatt                                                                                  <  1:2074617/100‑6 (MQ=38)
                 tACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagattc                                                                                 <  2:1327082/100‑7 (MQ=38)
                  aCCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtctc                                                                                <  2:2459519/100‑1 (MQ=255)
                  aCCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtctc                                                                                >  2:2342131/1‑100 (MQ=255)
                  aCCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtctc                                                                                >  2:2563650/1‑100 (MQ=255)
                  aCCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtctc                                                                                >  2:528412/1‑100 (MQ=255)
                  aCCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTATTTTTGtctc                                                                                >  2:1698473/1‑100 (MQ=255)
                   ccAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTcc                                                                               >  2:2493402/1‑100 (MQ=255)
                    cAGAAGTTCTGCGTCCGTATATGAATGGACTAGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagattcaga                                                                              <  1:2055708/100‑10 (MQ=37)
                    cAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTccc                                                                              >  1:1725277/1‑100 (MQ=255)
                     aGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTTTTAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCt                                                                             >  1:1773215/1‑100 (MQ=255)
                     aGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCt                                                                             >  2:2313201/1‑100 (MQ=255)
                     aGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagattcagaa                                                                             <  1:1052465/100‑11 (MQ=37)
                      gAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCtt                                                                            >  2:2302774/1‑100 (MQ=255)
                      gAAGTTCTGCGTCCGTATATGAACGGACTCGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCCCCTGCGGGCGCTTTTTTTGTCTCCCtt                                                                            <  1:1819605/100‑1 (MQ=255)
                       aaGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCttt                                                                           <  2:208355/100‑1 (MQ=255)
                       aaGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCttt                                                                           <  2:769705/100‑1 (MQ=255)
                             tGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATAcc                                                                     >  1:2605968/1‑100 (MQ=255)
                              gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                    >  2:2648371/1‑100 (MQ=255)
                               cGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGa                                                                   <  2:1106644/100‑1 (MQ=255)
                                 tCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAAc                                                                 <  1:1346968/100‑1 (MQ=255)
                                       tatGAACGGACTGGAATATATTGGCTAATCCCCAATTTTTCTGACTATAACAAGCGCCTGCGGGCGCTGTTTTTGTCTCTCTTTTATACCg                                                                    >  1:757783/1‑91 (MQ=255)
                                       tatGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaataat                                                           <  2:1691341/100‑1 (MQ=255)
                                       tatGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaataat                                                           <  1:406753/100‑1 (MQ=255)
                                        atGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAAtt                                                          >  2:2321415/1‑100 (MQ=255)
                                        atGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAAtt                                                          <  1:511927/100‑1 (MQ=255)
                                         tGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTa                                                         >  2:2847733/1‑100 (MQ=255)
                                         tGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTa                                                         >  1:2503735/1‑100 (MQ=255)
                                          gAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTAc                                                        <  2:1398786/100‑1 (MQ=255)
                                          gAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTAc                                                        <  2:308712/100‑1 (MQ=255)
                                          gAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTGTCTCCCTTTGATACCGAACAATAATTAct                                                       >  1:75972/1‑100 (MQ=255)
                                             cGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcc                                                     <  2:1079899/100‑1 (MQ=255)
                                              ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcct                                                    >  1:333696/1‑100 (MQ=255)
                                              ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcc                                                     <  1:1365137/99‑1 (MQ=255)
                                               gACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcctc                                                   <  2:114979/100‑1 (MQ=255)
                                                aCTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcct                                                    <  1:1848881/98‑1 (MQ=255)
                                                 cTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCAc                                                 <  2:1820676/100‑1 (MQ=255)
                                                   ggAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACtt                                               >  2:1321243/1‑100 (MQ=255)
                                                   ggAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACtt                                               >  1:991245/1‑100 (MQ=255)
                                                   ggAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACtt                                               >  2:1297618/1‑100 (MQ=255)
                                                   ggAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACtt                                               >  2:2092147/1‑100 (MQ=255)
                                                   ggAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACtt                                               <  2:1685235/100‑1 (MQ=255)
                                                   ggAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACtt                                               >  2:884494/1‑100 (MQ=255)
                                                      atatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTaca                                            <  1:261691/100‑1 (MQ=255)
                                                        atatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACg                                          <  2:2169056/100‑1 (MQ=255)
                                                           ttGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTaa                                       <  2:865211/100‑1 (MQ=255)
                                                            tGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAt                                      <  2:2791542/100‑1 (MQ=255)
                                                             ggCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAta                                     >  2:2816200/1‑100 (MQ=255)
                                                               cTAATACCCAATTTTTCTGAATCTAAAATGCGCCTCCGGGCGCTTTTTTTGTCGCCCTTTGATACCTAACACTAATTACTCATCACTTACACGTAAtac                                    >  1:1642104/1‑99 (MQ=255)
                                                                tAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAtacta                                  >  2:483814/1‑100 (MQ=255)
                                                                     cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTc                             >  2:2366465/1‑100 (MQ=255)
                                                                     cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTc                             >  2:1247173/1‑100 (MQ=255)
                                                                     cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTc                             >  1:1537479/1‑100 (MQ=255)
                                                                      ccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCg                            <  2:1784959/100‑1 (MQ=255)
                                                                       cgaTTTTTCTGAAACTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTTATACCGAACAATAATTACTCCTCACTTACACGTAGTACTACTTTcta                           >  2:1977835/3‑98 (MQ=255)
                                                                       cAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGCACCATTGTTACTCCACACTTACACCAAACACTACTCTCg                            >  1:221424/1‑99 (MQ=255)
                                                                       cAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTACCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGa                           >  1:1613975/1‑100 (MQ=255)
                                                                       cAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAg                          >  2:912173/1‑100 (MQ=255)
                                                                        aaTTTTTCTGAATCTCATATGCGCCGGCGGGCGTTTTCTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAg                          <  1:815273/100‑1 (MQ=255)
                                                                        aaTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAg                          <  1:694777/100‑1 (MQ=255)
                                                                           ttttCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGa                       <  2:1736645/100‑1 (MQ=255)
                                                                             ttCTGAATCTAAACAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCCAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGaaa                     <  2:274920/100‑1 (MQ=255)
                                                                              tCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGaaaa                    <  1:1506689/100‑1 (MQ=255)
                                                                              tCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGaaaa                    >  2:291711/1‑100 (MQ=255)
                                                                                gagacaaaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATc                  >  1:1717582/8‑100 (MQ=38)
                                                                                 gAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATc                  <  1:1281039/99‑1 (MQ=255)
                                                                                   acaaaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTAc               >  1:1767571/5‑100 (MQ=39)
                                                                                   aTCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGCGTGAAAATCTAc               <  2:1917547/100‑1 (MQ=255)
                                                                                   aTCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAAGCTAc               >  2:1250537/1‑100 (MQ=255)
                                                                                    tCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTTCTTTCGAGTGAAAATCTAcc              <  1:1787459/100‑1 (MQ=255)
                                                                                    tCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTAcc              <  1:2383846/100‑1 (MQ=255)
                                                                                    caaaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTAcc              >  1:1381432/4‑100 (MQ=255)
                                                                                     aaaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCt             >  2:1786490/3‑100 (MQ=255)
                                                                                     aaaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCt             >  1:1179054/3‑100 (MQ=255)
                                                                                      tAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATCATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTa            >  1:286449/1‑100 (MQ=255)
                                                                                      tAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTACTACTACTTTTGAGTGAAAATATACCTa            >  1:1304589/1‑100 (MQ=255)
                                                                                      aaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTa            >  2:2202333/2‑100 (MQ=255)
                                                                                           aGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATctct       >  2:162590/1‑100 (MQ=255)
                                                                                             cgcCTGCGGGCGCTTTTTTTTTTTTTTTTTAATACCAAACAATAATTACGCCTCACTTACACTTGATATTACTTTCGATTGAAAATCTACCTATCTCtt      >  1:1697929/1‑99 (MQ=255)
                                                                                               ccTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGa   <  1:804569/100‑1 (MQ=255)
                                                                                                cTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt  <  1:1321238/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
TGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAT  >  NC_000913/940654‑940849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: