Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 87,887 C→T 17.3% intergenic (+39/‑141) ilvH → / → cra acetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091387,8870CT17.3% 271.5 / 44.5 121intergenic (+39/‑141)ilvH/craacetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand):  ref base C (42/57);  new base T (13/8);  total (56/65)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01

Only 100 of 125 total aligned reads displayed.

TGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAG  >  NC_000913/87788‑87986
                                                                                                   |                                                                                                    
tgtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCAc                                                                                                      >  1:209425/1‑100 (MQ=255)
 gtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACg                                                                                                     >  1:857134/1‑100 (MQ=255)
    gAGGTTGCTCGCTCTGGTGTGGACGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTg                                                                                                  >  1:1690757/1‑100 (MQ=255)
      ggTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg                                                                                                 >  1:695306/1‑99 (MQ=255)
       gTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGTCATGTTGGGc                                                                                               <  2:510193/100‑1 (MQ=255)
       gTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGc                                                                                               <  2:86166/100‑1 (MQ=255)
       gTTGCTCGCTCTGGGGTGGTCGGACTTTCTCGCGGCGATAAAATTATGCGTTGGGAATTATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGc                                                                                               <  1:1594085/100‑1 (MQ=255)
        ttGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCt                                                                                             <  1:1235961/100‑1 (MQ=255)
         tGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                            <  1:2236108/100‑2 (MQ=255)
         tGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                            <  2:312840/100‑2 (MQ=255)
         tGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                            <  1:1877989/100‑2 (MQ=255)
         tGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtt                                                                                            >  2:2135910/1‑100 (MQ=255)
           ctcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAACGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtgt                                                                                           <  1:2429127/99‑3 (MQ=38)
             cgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttttt                                                                                        >  2:339474/1‑100 (MQ=255)
              gctcTGGTGTGGTCGAACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtgtaaat                                                                                       <  2:2869870/100‑7 (MQ=37)
               ctctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTg                                                                                      <  1:1116008/100‑1 (MQ=255)
                  tGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                    <  1:2616969/99‑1 (MQ=255)
                   ggTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa                                                                                  <  1:2085628/100‑1 (MQ=255)
                   ggTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTGGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa                                                                                  <  1:1243031/100‑1 (MQ=255)
                      gtgGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCttttttttcttatt                                                                                >  1:1851260/1‑92 (MQ=255)
                      gtgGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc                                                                               <  2:195388/100‑1 (MQ=255)
                        ggTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttttttttttttt                                                                                >  1:597667/1‑90 (MQ=255)
                        ggTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAg                                                                             >  2:1572990/1‑100 (MQ=255)
                          tCGGACTTTCGCGCTGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                           <  1:1702178/100‑1 (MQ=255)
                          tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                           <  1:2491890/100‑1 (MQ=255)
                          tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                           <  2:1007414/100‑1 (MQ=255)
                          tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                           <  2:2566135/100‑1 (MQ=255)
                          tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                           <  2:310276/100‑1 (MQ=255)
                           cGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTgg                                                                          <  2:912373/100‑1 (MQ=255)
                            ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTggg                                                                         <  1:539764/100‑1 (MQ=255)
                             gACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGa                                                                        >  1:983073/1‑100 (MQ=255)
                               cTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAAc                                                                      >  2:954837/1‑100 (MQ=255)
                                tttCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAAcc                                                                     <  2:1783790/100‑1 (MQ=255)
                                   cgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTgg                                                                  <  1:2791951/100‑1 (MQ=255)
                                   cgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTgg                                                                  >  2:2050698/1‑100 (MQ=255)
                                    gcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTgg                                                                  <  2:1492324/99‑1 (MQ=255)
                                     cgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGaa                                                                <  2:981950/100‑1 (MQ=255)
                                          cgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaaaa                                                           <  2:778842/100‑1 (MQ=255)
                                           gATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg                                                          >  1:2742984/1‑100 (MQ=255)
                                           gATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg                                                          <  2:2474459/100‑1 (MQ=255)
                                            aTAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg                                                          <  1:1667126/100‑1 (MQ=255)
                                            aTAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGc                                                         <  1:889363/100‑1 (MQ=255)
                                            aTAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGc                                                         >  1:923558/1‑100 (MQ=255)
                                             tAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg                                                          <  1:185589/98‑1 (MQ=255)
                                              aaaaTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAg                                                       <  2:1242010/100‑1 (MQ=255)
                                              aaaaTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAg                                                       <  2:1865579/100‑1 (MQ=255)
                                              aaaaTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAg                                                       >  2:751203/1‑100 (MQ=255)
                                               aaaTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGt                                                      >  1:154025/1‑100 (MQ=255)
                                                     tGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGc                                                >  2:2791810/1‑100 (MQ=255)
                                                      gCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCg                                                 <  1:2036370/98‑1 (MQ=255)
                                                      gCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCa                                               <  2:951955/100‑1 (MQ=255)
                                                      gCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCa                                               >  2:857601/1‑100 (MQ=255)
                                                       cGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAg                                              >  2:1997162/1‑100 (MQ=255)
                                                        gTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGt                                             >  2:763764/1‑100 (MQ=255)
                                                             gaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGCAATAAAAGCAGTTGCCGCAGTTAAtt                                        >  1:2416876/1‑100 (MQ=255)
                                                               aTGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAAtttt                                      <  2:1571436/100‑1 (MQ=255)
                                                                tGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTc                                     <  2:833517/100‑1 (MQ=255)
                                                                 gATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCt                                    >  1:662749/1‑100 (MQ=255)
                                                                 gATCTCAATGCGCAATTTACAGCCCAACATGTCAAGTTGGGCTTTTTTTGCGAAATCAGTGGGAACATGGAATAAAAGCAGTTGCCGCAGTTAATTTTCt                                    >  2:1675176/1‑100 (MQ=255)
                                                                   tctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgc                                  >  2:623892/1‑100 (MQ=255)
                                                                   tctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgc                                  >  2:1496932/1‑100 (MQ=255)
                                                                   tctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgc                                  <  1:1151849/100‑1 (MQ=255)
                                                                    ctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgcg                                 <  2:81258/100‑1 (MQ=255)
                                                                      cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTTTTTAATCAGTGAGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTTCGCt                               >  1:2217911/1‑100 (MQ=255)
                                                                      cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCt                               <  1:1638549/100‑1 (MQ=255)
                                                                      cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCt                               >  1:1486953/1‑100 (MQ=255)
                                                                       aaTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCt                               >  1:2692519/1‑99 (MQ=255)
                                                                        aTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCtt                              <  1:662970/99‑1 (MQ=255)
                                                                        aTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTa                             <  2:347617/100‑1 (MQ=255)
                                                                        aTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTa                             <  2:1170790/100‑1 (MQ=255)
                                                                          gcgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGa                           <  1:2883755/100‑1 (MQ=255)
                                                                          gcgcAATTTACAGCCAAACATGGCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTGAATTTTCTGCGCTTAg                            <  1:696925/99‑1 (MQ=255)
                                                                           cgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGAt                          <  1:2135897/100‑1 (MQ=255)
                                                                             gcaaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCAAAATCAGTGGGAACCAGGCATAAAAACAGTTACCGCAGGTAAGTTTCAGCGCTTAGa                           >  1:2411184/9‑97 (MQ=16)
                                                                              cttccgatctccAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                       <  2:1604453/90‑1 (MQ=255)
                                                                              caaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                       >  2:2401847/8‑100 (MQ=37)
                                                                              caaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                       >  1:2208640/8‑100 (MQ=38)
                                                                              caaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                       >  2:1918860/8‑100 (MQ=37)
                                                                              aaTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCTCAGTTAATTTTCTGCGCTTAGATGtt                       <  2:597670/100‑1 (MQ=255)
                                                                              aaTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                       >  2:1008786/1‑100 (MQ=255)
                                                                              aaTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                       <  1:1997151/100‑1 (MQ=255)
                                                                               aaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTa                      >  2:1702909/7‑100 (MQ=38)
                                                                                 ttACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGGTGTTAAt                    <  2:1115489/100‑1 (MQ=255)
                                                                                    cAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGaa                 <  2:1590271/100‑1 (MQ=255)
                                                                                     ngCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAt                >  2:1533219/2‑100 (MQ=255)
                                                                                     aGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAt                >  2:1261552/1‑100 (MQ=255)
                                                                                      gCCCAACGTGACATGTTGGGCTTTTTTTTTTAATTCATTGGGAACCTGGAATAAAAGCAGTTGCCTCAGTTAATTTTCTGCGCTTAGATGTTAATGAAtt               >  1:2892328/1‑100 (MQ=255)
                                                                                      gCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAtt               >  2:680413/1‑100 (MQ=255)
                                                                                      gCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAtt               <  2:2217707/100‑1 (MQ=255)
                                                                                      gCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAtt               <  2:299863/100‑1 (MQ=255)
                                                                                        ccAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTagatcggaag             >  1:1604447/1‑91 (MQ=255)
                                                                                          aaCATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAACAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAc           <  2:57952/100‑1 (MQ=255)
                                                                                          aaCATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAc           <  2:377390/100‑1 (MQ=255)
                                                                                            cATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAccc         <  2:2035430/100‑1 (MQ=255)
                                                                                              tGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCAt       <  2:1478256/100‑1 (MQ=255)
                                                                                               gTCACGTTGGGCTTTTTTTTCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATa      >  1:918317/1‑100 (MQ=255)
                                                                                                tCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATAc     >  2:852547/1‑100 (MQ=255)
                                                                                                   cGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAg  >  2:100021/1‑100 (MQ=255)
                                                                                                   cGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAg  >  2:28890/1‑100 (MQ=255)
                                                                                                   cGTTGGGCTTTTTTTGCGAAATAAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACACATAcc    >  1:1939783/1‑98 (MQ=255)
                                                                                                   |                                                                                                    
TGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAG  >  NC_000913/87788‑87986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: