Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,246,073	C→T	100%	G435D (GGC→GAC)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,246,073	0	C	T	100.0%	280.5 / NA	83	G435D (GGC→GAC)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/34);  total (49/34)

AGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCC  >  NC_000913/1245978‑1246168                                                                                                |                                                                                                aGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgtcgcc                                                                                             <  1:711393/100‑1 (MQ=255)  gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGtcgccg                                                                                            <  2:477729/100‑1 (MQ=255)   ttttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCgg                                                                                           <  1:2093122/100‑1 (MQ=255)    tttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCgg                                                                                           >  1:1021863/1‑99 (MQ=255)     ttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGt                                                                                         >  2:2399625/1‑100 (MQ=255)     ttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGt                                                                                         <  1:164637/100‑1 (MQ=255)      tGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGtt                                                                                        >  2:2288251/1‑100 (MQ=255)       gTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTg                                                                                       >  1:1129841/1‑100 (MQ=255)       gTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTg                                                                                       <  2:124158/100‑1 (MQ=255)        tAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGtttc                                                                                      <  2:1963978/100‑3 (MQ=255)        tAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgc                                                                                      >  2:1710999/1‑100 (MQ=255)         aaTCCTTCTGTCGCGACCCACTGTAAGGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgca                                                                                     >  2:1773165/1‑100 (MQ=255)              ttCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGa                                                                                >  2:971328/1‑100 (MQ=255)               tCTGTCGAGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAt                                                                               >  2:1523671/1‑100 (MQ=255)                cTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtg                                                                              <  2:2179338/100‑1 (MQ=255)                cTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtg                                                                              <  1:723091/100‑1 (MQ=255)                cTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATACCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtg                                                                              <  1:1665861/100‑1 (MQ=255)                 tGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtgt                                                                             >  2:1675878/1‑100 (MQ=255)                    cgcgACCCACTGTAACGGTGCCCAGCAATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGtt                                                                          >  1:114889/1‑100 (MQ=255)                      cgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGtttt                                                                        <  2:905498/100‑1 (MQ=255)                        aCCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcg                                                                      <  2:1266187/100‑1 (MQ=255)                        aCCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcg                                                                      >  2:1036920/1‑100 (MQ=255)                        aCCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcg                                                                      >  1:336167/1‑100 (MQ=255)                          ccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtc                                                                    >  2:2194604/1‑100 (MQ=255)                                   cGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCgtcgccgtcgcc                                                           >  1:2309737/1‑100 (MQ=255)                                   cGGTGCCCAGCCATTTGGCGCAACCAATTGTTGCACACTATTCACCGACGAGGTGTTCAGGTCGCCGAGCTACAGCAGAAGTGTTTTCgtcgcagtcgcc                                                           >  1:2171864/1‑100 (MQ=255)                                    ggTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCa                                                          >  1:1682128/1‑100 (MQ=255)                                     gTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTTTTTTTTTTTTTTTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtcgc                                                            >  1:803079/1‑97 (MQ=255)                                     gTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAt                                                         >  2:1546811/1‑100 (MQ=255)                                        cccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtcgac                                                           >  1:1968576/1‑93 (MQ=255)                                        cccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtttt                                                      >  2:2211811/1‑100 (MQ=255)                                        cccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtttt                                                      >  1:684420/1‑100 (MQ=255)                                         ccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                                     >  2:402920/1‑100 (MQ=255)                                          cAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtttt                                                      <  1:2747416/98‑1 (MQ=255)                                            gCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgttg                                                  >  2:529662/1‑100 (MQ=255)                                            gCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgttg                                                  <  2:1150071/100‑1 (MQ=255)                                             ccATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTgg                                                 >  2:1578707/1‑100 (MQ=255)                                              cATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCCGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgc                                                >  2:2107389/1‑100 (MQ=255)                                              cATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgc                                                >  2:2288414/1‑100 (MQ=255)                                              cATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgc                                                >  2:2327056/1‑100 (MQ=255)                                                tttGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcgc                                              <  2:1594332/100‑1 (MQ=255)                                                tttGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcgc                                              <  2:2377470/100‑1 (MQ=255)                                                 ttGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGcgcg                                             <  2:1107804/100‑1 (MQ=255)                                                   ggCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                           <  2:2528546/100‑1 (MQ=255)                                                   ggCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                           <  2:1620796/100‑1 (MQ=255)                                                    gcgcATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATc                                          <  2:146142/100‑1 (MQ=255)                                                     cgcATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCt                                         <  1:62545/100‑1 (MQ=255)                                                     cgcATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCt                                         >  2:544543/1‑100 (MQ=255)                                                      gcATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCtt                                        <  1:546762/100‑1 (MQ=255)                                                        aTCCCATTGTTGCCCACTTTTCACCGACGTGGTGTCCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCttt                                       >  1:1411692/1‑99 (MQ=255)                                                        aTCCCATTGTTGCCCACTTTTCACCGACGTGGTGTCAATATCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCAGTCGCTATTTTGTTGGCGCGATCttta                                      <  1:943922/100‑2 (MQ=255)                                                           ccATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCt                                   <  1:1710992/100‑1 (MQ=255)                                                             aTTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGc                                 >  1:82104/1‑100 (MQ=255)                                                              ttgttgCCCACTTTTCACCGACGTTGGGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGCCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGcc                                <  1:2159431/100‑1 (MQ=255)                                                               tgttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCg                               <  1:227472/100‑1 (MQ=255)                                                                gttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGc                              <  1:2593302/100‑1 (MQ=255)                                                                gttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCAATTTTGTTGGCGCGATCTTTCGCTGc                                 >  1:18810/1‑97 (MQ=255)                                                                 ttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGc                              >  1:2089933/1‑99 (MQ=255)                                                                   gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTTGCTCGATCTTTCGCTGCCGCAt                            >  2:438805/1‑99 (MQ=255)                                                                   gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAtt                           >  1:1336780/1‑100 (MQ=255)                                                                   gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAtt                           >  2:626694/1‑100 (MQ=255)                                                                   gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAtt                           <  2:2047761/100‑1 (MQ=255)                                                                     ccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGa                         <  1:1335304/100‑1 (MQ=255)                                                                        cTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACg                       >  1:2416884/1‑99 (MQ=255)                                                                           ttCACCGACGTGGTGTTCAGGTCGCCGGGGTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAGCTTTCGCTGCCGCATTGACGTACa                   >  2:584336/1‑100 (MQ=255)                                                                              aCCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCg                >  2:1440214/1‑100 (MQ=255)                                                                               ccGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCgg               >  1:191131/1‑100 (MQ=255)                                                                               ccGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCgg               <  2:2070972/100‑1 (MQ=255)                                                                               ccGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCgg               >  1:2118033/1‑100 (MQ=255)                                                                                   cGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAAc           <  2:2619239/100‑1 (MQ=255)                                                                                   cGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAAc           >  1:2308836/1‑100 (MQ=255)                                                                                      ggtgTTCAGGTCGCCGGGTTGCAGCAGCTGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAgg        <  1:2325607/100‑1 (MQ=255)                                                                                       gtgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAggg       <  2:1533604/100‑1 (MQ=255)                                                                                        tgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggc      >  2:212143/1‑100 (MQ=255)                                                                                        tgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggc      >  2:2376740/1‑100 (MQ=255)                                                                                         gtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcg     <  2:1760671/100‑1 (MQ=255)                                                                                         gtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcg     >  2:2656731/1‑100 (MQ=255)                                                                                         gtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcg     <  1:1954067/100‑1 (MQ=255)                                                                                         gtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcg     >  1:2421654/1‑100 (MQ=255)                                                                                         gtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCAGCATTGACGTACAGCGGGCATAGggcg     >  1:128248/1‑100 (MQ=255)                                                                                          ttCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCTCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcg     <  1:1869304/99‑1 (MQ=255)                                                                                          ttCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcgg    <  2:2885844/100‑1 (MQ=255)                                                                                           tCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcgg    >  1:2798977/1‑99 (MQ=255)                                                                                            cAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGcc  >  1:1673963/1‑100 (MQ=255)                                                                                            cAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGcc  >  2:2153718/1‑100 (MQ=255)                                                                                                |                                                                                                AGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCC  >  NC_000913/1245978‑1246168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑