Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,666,470 C→A 23.2% L386I (CTA→ATA)  clcB → H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,666,4700CA23.2% 247.8 / 69.9 116L386I (CTA→ATA) clcBH(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base C (49/40);  new base A (14/13);  total (63/53)
Fisher's exact test for biased strand distribution p-value = 8.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 117 total aligned reads displayed.

TTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGC  >  NC_000913/1666371‑1666568
                                                                                                   |                                                                                                  
ttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGc                                                                                                    <  2:1618519/100‑1 (MQ=255)
 tACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCt                                                                                                   <  2:343610/100‑1 (MQ=255)
   cTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCCCGCGCCGATTATGTCGACGTTGAGGATATGTGAAATGACCGGGGAGTATCAGCTAc                                                                                                 >  1:1405158/1‑100 (MQ=255)
   cTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTAc                                                                                                 >  2:1022743/1‑100 (MQ=255)
   cTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGGTGATGATATGTGAAATGACCGGGGAGTATCAGCTa                                                                                                  >  1:642086/1‑99 (MQ=255)
     cGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTc                                                                                               >  1:824546/1‑100 (MQ=255)
        aTTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTcccc                                                                                            <  2:2380880/100‑1 (MQ=255)
        aTTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTcccc                                                                                            <  2:2608374/100‑1 (MQ=255)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGAGGTTGATGATATGTGAAATGACCGAGGAGTAGCTGATACTCCCTgg                                                                                          <  1:367655/100‑1 (MQ=255)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCgg                                                                                          <  2:2119664/100‑1 (MQ=255)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCgg                                                                                          <  2:451756/100‑1 (MQ=255)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATGCTCCCCgg                                                                                          <  1:593049/100‑1 (MQ=255)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCgg                                                                                          <  2:2436342/100‑1 (MQ=39)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCgg                                                                                          <  2:1792048/100‑1 (MQ=39)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCgg                                                                                          <  1:1263877/100‑1 (MQ=39)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACACACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCgg                                                                                          <  2:2637407/100‑1 (MQ=255)
           gACCTGGATTGCGACACTGTTGGCCGCAACCACTCACGCTCCGATTATGTCGACGTTGCTTATATTTGCAATGACCGGGGGGTAGCTGATACTCCCCgg                                                                                          <  1:1914377/99‑1 (MQ=255)
           gACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGt                                                                                         >  2:515344/1‑100 (MQ=255)
           gACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGt                                                                                         >  2:1644683/1‑100 (MQ=255)
           gACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCGGt                                                                                         <  2:2723823/100‑1 (MQ=39)
           gACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGAGGACATGTGAAATGACCGGGGAGTATCAGCTACTCCCCgg                                                                                          >  1:938444/1‑99 (MQ=255)
              cGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTa                                                                                      >  2:1245168/1‑100 (MQ=255)
               gggATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTat                                                                                     >  2:465134/1‑100 (MQ=255)
                    ggCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgatt                                                                                >  2:2720506/1‑100 (MQ=255)
                    ggCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgatt                                                                                <  2:1640739/100‑1 (MQ=255)
                     gCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCGGtcatttcacat                                                                               <  2:1847746/100‑11 (MQ=25)
                     ccgatcttGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgattg                                                                               <  2:902675/93‑1 (MQ=255)
                      cGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGc                                                                              >  1:2857409/1‑100 (MQ=255)
                       gACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGcc                                                                             <  2:2568576/100‑1 (MQ=255)
                       gACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTCGCTGATATGCGAAATGACCGGGGAGCAGCCGCTACTCCCCGGTTTATGGATTGc                                                                              >  1:2486350/1‑99 (MQ=255)
                           cTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCg                                                                         <  2:854934/100‑1 (MQ=255)
                           cTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCg                                                                         >  1:1045253/1‑100 (MQ=255)
                            tGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgattgagatcgg                                                                        >  1:902672/1‑93 (MQ=255)
                            tGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGt                                                                        <  1:225793/100‑1 (MQ=255)
                            tGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGt                                                                        <  2:1125541/100‑1 (MQ=255)
                             gTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTa                                                                       <  2:2588607/100‑1 (MQ=255)
                               tGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAAt                                                                     <  1:2214614/100‑1 (MQ=255)
                               tGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAAt                                                                     >  2:398685/1‑100 (MQ=255)
                                 gcggcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTg                                                                   <  2:231423/100‑1 (MQ=255)
                                 gcggcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTg                                                                   <  1:1293521/100‑1 (MQ=255)
                                 gcggcgACCACGCACGCGCCGATTATGTCGACGTCGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTg                                                                   <  1:1220388/100‑1 (MQ=255)
                                   ggcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATTACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCGGCGTAATTGCg                                                                 <  1:488555/100‑1 (MQ=255)
                                     cgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTc                                                               <  1:1677622/100‑1 (MQ=255)
                                       aCCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCgg                                                             <  1:2216573/100‑1 (MQ=255)
                                          acgcacgcGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTaa                                                          >  1:2332169/1‑100 (MQ=255)
                                           cgcacgcGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAAt                                                         >  2:303631/1‑100 (MQ=255)
                                           cgcacgcGCCGATTATGTCAAAGTTGATGATGGGTGAAATGGCGGGGGAGTATCAGCTACTCCCAGTTTTATTGATTGCCTGCGTAAGTGCGACGGTAAt                                                         >  1:1763492/1‑100 (MQ=255)
                                             cacgcGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAAttt                                                       >  2:1992651/1‑100 (MQ=255)
                                               cgcgCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcg                                                     <  2:2180270/100‑1 (MQ=255)
                                                gcgcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgc                                                    >  2:2039407/1‑100 (MQ=255)
                                                 cgcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTGATTTcgcg                                                   >  1:2057842/1‑100 (MQ=255)
                                                  gcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCgg                                                  <  2:2450346/100‑1 (MQ=255)
                                                    cGATTCTGTCGCCATTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGa                                                 >  1:1804261/1‑99 (MQ=255)
                                                    cGATTATGTCGACGTTGATGATATGTGAAATTACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg                                                   <  2:2321063/97‑1 (MQ=255)
                                                     gATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACg                                               >  2:1647395/1‑100 (MQ=255)
                                                      aTTATGTCGACGTTGATGATATGTGAAATGACCGGGGCGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCAGACGt                                              <  1:1967595/100‑1 (MQ=255)
                                                       ttATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGtt                                             >  1:933433/1‑100 (MQ=255)
                                                           gTCGACGTTGATGATATGTGAAATGACTGGGGAGTATCAGCTACTCCCCGGTTTATTTATTGCCTGCGTAATTGCGTCGGTACTTTCGCGGACGTTac                                           >  1:1897579/1‑98 (MQ=255)
                                                           gTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTacac                                         <  1:2610226/100‑1 (MQ=255)
                                                           gTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTacac                                         >  2:322591/1‑100 (MQ=255)
                                                                cGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGa                                    <  2:1342172/100‑1 (MQ=255)
                                                                    gatgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGActct                                >  2:1901643/1‑100 (MQ=255)
                                                                     atgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtcta                               <  2:2733641/100‑1 (MQ=255)
                                                                       gatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatc                             <  2:2158169/100‑1 (MQ=255)
                                                                       gatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatc                             <  1:1881310/100‑1 (MQ=255)
                                                                       gatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatc                             <  2:635951/100‑1 (MQ=255)
                                                                        agatGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatct                            >  2:1978384/3‑100 (MQ=255)
                                                                          atGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTAc                          >  1:1691415/1‑100 (MQ=255)
                                                                           tgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTAcc                         >  1:1163698/1‑100 (MQ=255)
                                                                           tgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTAcc                         >  2:2896746/1‑100 (MQ=255)
                                                                            gtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCg                        >  2:2172795/1‑100 (MQ=255)
                                                                            atcaataaaCCGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCg                        >  1:49703/9‑100 (MQ=37)
                                                                              ccgatctccGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGcn                      <  1:1278323/93‑2 (MQ=38)
                                                                              caataaaCCGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGcc                      >  2:1657193/7‑100 (MQ=37)
                                                                               aaaTGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGcn                      <  1:2379296/99‑2 (MQ=255)
                                                                               aaaTGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCa                     <  2:2204829/100‑1 (MQ=255)
                                                                                  aaaCCGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGca                  >  2:1340662/3‑100 (MQ=38)
                                                                                    aCCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGcaca                >  1:134825/1‑100 (MQ=255)
                                                                                     ccGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  1:1446807/1‑100 (MQ=255)
                                                                                     ccGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAgatcgg               >  2:1278328/1‑95 (MQ=38)
                                                                                     ccGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  1:2362865/1‑100 (MQ=39)
                                                                                     ccGGGGAGTAGCTGATACTCCCCGGTTTAGTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  1:175025/1‑100 (MQ=39)
                                                                                      cGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACt              >  2:408122/1‑100 (MQ=255)
                                                                                      cGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACt              >  2:442896/1‑100 (MQ=255)
                                                                                       ggggAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACt              >  1:1741733/1‑99 (MQ=255)
                                                                                       ggggAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTg             >  1:2840953/1‑100 (MQ=255)
                                                                                       ggggAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTg             >  1:1100350/1‑100 (MQ=255)
                                                                                       ggggAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTg             >  1:1437485/1‑100 (MQ=255)
                                                                                       ggggAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTg             >  2:1182521/1‑100 (MQ=255)
                                                                                       ggggAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTg             >  2:161778/1‑100 (MQ=255)
                                                                                        gggAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTgc            <  2:2320324/100‑1 (MQ=255)
                                                                                         ggAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTTCGTCGGTAATTTCGCGGACGTTCCACCGTGACTCTGTCTACCGCCAGCacacgg             >  1:548195/1‑96 (MQ=255)
                                                                                           aGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgca         <  2:834340/100‑1 (MQ=255)
                                                                                            gTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcag        <  1:1933108/100‑1 (MQ=255)
                                                                                            gTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcag        >  2:866105/1‑100 (MQ=255)
                                                                                             tATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcagc       <  2:824549/100‑1 (MQ=255)
                                                                                              aTCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcagca      <  2:2226272/100‑1 (MQ=255)
                                                                                                 aGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAg   >  1:2114792/1‑100 (MQ=255)
                                                                                                 aGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAg   >  1:2730020/1‑100 (MQ=255)
                                                                                                 aGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAg   <  1:1170727/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGC  >  NC_000913/1666371‑1666568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: