Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,755,639	G→A	100%	intergenic (‑498/‑59)	ydhZ ← / → pykF	uncharacterized protein/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,639	0	G	A	100.0%	250.6 / ‑6.7	80	intergenic (‑498/‑59)	ydhZ/pykF	uncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (51/28);  minor base T (1/0);  total (52/28)

GAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACC  >  NC_000913/1755544‑1755732                                                                                                |                                                                                              gagaCACAAGCACACATTCCTCTGCACGCTTTTTTTTTTTTTTTTATCCTTAGAGCGAGGCACCACCACTTTTGTAATACCGGATTCGCTTTCCGTCAGt                                                                                           >  1:2555879/1‑100 (MQ=255)  agaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTg                                                                                          <  2:708606/100‑1 (MQ=255)  agaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTg                                                                                          <  2:1489305/100‑1 (MQ=255)   gaCACAAGCACACATTCCTCTGCACGCTTTTTTGATGTTACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                         >  1:2272183/1‑100 (MQ=255)   gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                         >  2:2394891/1‑100 (MQ=255)   gaCACAAGCACACATTCCTCTGCACGCTTTCTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGtac                                                                                         >  1:117530/1‑98 (MQ=255)     cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                       >  2:2646377/1‑100 (MQ=255)       caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGccc                                                                                     >  2:2249738/1‑100 (MQ=255)       caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGccc                                                                                     <  2:2577295/100‑1 (MQ=255)         aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                   >  2:308947/1‑100 (MQ=255)         aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                   <  2:2837697/100‑1 (MQ=255)         aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                   >  2:1476927/1‑100 (MQ=255)            acacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGaaa                                                                                >  1:880480/1‑100 (MQ=255)             cacaTTCCTCTGCACGCTTTTTTTTTTTTTTTTTTCCTTAGAGCGAGGCACCACCACTTTCTTAATGCCGGATTCGCTTTCCGACAGTGCGcc                                                                                      >  1:199282/1‑93 (MQ=255)             cacaTTCCTCTGCACGCTTTTTTTTTTTTTTTTATCCTTAGAGCGAGGCACCACCACTTTTGTAATACCGGATTCGCTTTCCGACAGTCCGCCCAGAAAg                                                                               >  1:2796417/1‑100 (MQ=255)               caTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCa                                                                             <  1:763291/100‑1 (MQ=255)                 ttCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAg                                                                           >  1:2209049/1‑100 (MQ=255)                 tgtctctGCGCGGTTTTTCTTTGTCAGCTATCGTTAGAGCGAGGCATCATCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg                                                                               <  1:2422364/93‑1 (MQ=255)                  tCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGt                                                                          <  1:2014856/100‑1 (MQ=255)                  tCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGt                                                                          >  2:401956/1‑100 (MQ=255)                  tCCCCTGCACGCTTTTGCGATGTCACCTATCGTTAGAGCGAGGCACCACCCCTTTCATAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAg                                                                           <  1:1441428/99‑1 (MQ=255)                      ctGCACGCTTTTTTTTTTTTTCCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtct                                                                      >  1:2224272/1‑100 (MQ=255)                       tGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtctc                                                                     >  2:248682/1‑100 (MQ=255)                        gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                    >  2:2260362/1‑100 (MQ=255)                         cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                   <  1:1741311/100‑1 (MQ=255)                         cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                   >  2:659608/1‑100 (MQ=255)                           cGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCAt                                                                 <  1:1607205/100‑1 (MQ=255)                           cGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCAt                                                                 >  2:1815286/1‑100 (MQ=255)                            gCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCTATc                                                                >  1:1826877/1‑100 (MQ=255)                              tttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCt                                                              >  2:2028891/1‑100 (MQ=255)                                tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc                                                            >  1:710094/1‑100 (MQ=255)                                tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc                                                            <  2:1510568/100‑1 (MQ=255)                                tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc                                                            >  2:1276009/1‑100 (MQ=255)                                   cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa                                                         >  2:2527640/1‑100 (MQ=255)                                   cGATGACACCTCTCCTTAGAGCGAGGCACCTCCGCTTTCGTAATACCGGATTCGCTTTCCGACCGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa                                                         <  1:1738421/100‑1 (MQ=255)                                    gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCaa                                                        <  1:464430/100‑1 (MQ=255)                                    gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGACTAGCTTTCCGACAGTGCGCCCAGCAAGCAAGTTTCTCCCATCCTTCTCaa                                                        >  1:1871687/1‑100 (MQ=255)                                     aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc                                                       >  1:1359875/1‑100 (MQ=255)                                     aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc                                                       >  1:1359618/1‑100 (MQ=255)                                      tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt                                                      >  1:230473/1‑100 (MQ=255)                                       gTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACtt                                                     >  2:353292/1‑100 (MQ=255)                                        tCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa                                                    >  2:1297055/1‑100 (MQ=255)                                        tCACCTATCCTTAGAGCGAGGCACCACCACTGTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa                                                    <  1:46587/100‑1 (MQ=255)                                         cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa                                                   >  2:906468/1‑100 (MQ=255)                                           ccTATCCTTAGAGCGAGGCACCACTACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                 <  2:833377/100‑1 (MQ=255)                                           ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                 >  2:505683/1‑100 (MQ=255)                                           ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                 >  1:1481336/1‑100 (MQ=255)                                           ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                 <  2:2000583/100‑1 (MQ=255)                                           catATCCTTAGACCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTACTCCCATCCTTCTCAACTTaaa                                                  >  1:1382859/3‑99 (MQ=255)                                                ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                            <  1:707809/100‑1 (MQ=255)                                                ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                            <  2:1810512/100‑1 (MQ=255)                                                 cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                           >  2:518667/1‑100 (MQ=255)                                                 cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                           >  2:434474/1‑100 (MQ=255)                                                 cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                           >  2:1794423/1‑100 (MQ=255)                                                 cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                           >  1:2843907/1‑100 (MQ=255)                                                   tAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagac                                         <  2:1048129/100‑1 (MQ=255)                                                     gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg                                       <  1:336629/100‑1 (MQ=255)                                                       gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTc                                     >  1:1048839/1‑100 (MQ=255)                                                       gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGAGCCCAGAAAGCAAATTTCTCACATCATTCTCAACTTAAAGACTAAGAATgcc                                     >  1:1303242/1‑98 (MQ=255)                                                        cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                    >  1:2541385/1‑100 (MQ=255)                                                        cGAGGCACCACCACTTTCGTAATAACGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                    >  1:1109277/1‑100 (MQ=255)                                                         gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCAt                                   <  2:1035003/100‑1 (MQ=255)                                                              accaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaa                              >  1:1447698/1‑100 (MQ=255)                                                                caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                            >  2:2476645/1‑100 (MQ=255)                                                                   cacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAcc                         <  1:2739078/100‑1 (MQ=255)                                                                     cTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaa                       >  2:1639514/1‑100 (MQ=255)                                                                     cTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaa                       >  1:383361/1‑100 (MQ=255)                                                                        tCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAt                    >  2:854313/1‑100 (MQ=255)                                                                            aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGtt                <  2:470309/100‑1 (MQ=255)                                                                             aTACCGGATTCGCTTTCCAACAGTGCGCCCAGAAAGCAAGTTTATCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt               <  1:2269255/100‑1 (MQ=255)                                                                              tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg              <  2:301328/100‑1 (MQ=255)                                                                               aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc             >  1:342123/1‑100 (MQ=255)                                                                               aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc             <  1:323441/100‑1 (MQ=255)                                                                                ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCa            >  2:1657889/1‑100 (MQ=255)                                                                                ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCa            >  1:678272/1‑100 (MQ=255)                                                                                  ggATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAACGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAcc          >  1:2391051/1‑100 (MQ=255)                                                                                  ggATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAcc          >  1:1371105/1‑100 (MQ=255)                                                                                  ggATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAcc          <  2:533164/100‑1 (MQ=255)                                                                                         cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGa    >  2:1235136/1‑99 (MQ=255)                                                                                          tttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAcc  <  1:260028/100‑1 (MQ=255)                                                                                                |                                                                                              GAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACC  >  NC_000913/1755544‑1755732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑