Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,764,687 T→C 20.8% intergenic (‑301/+247) sufA ← / ← ydiH Fe‑S cluster assembly protein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,764,6870TC20.8% 226.2 / 59.6 122intergenic (‑301/+247)sufA/ydiHFe‑S cluster assembly protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (55/41);  new base C (9/16);  total (65/57)
Fisher's exact test for biased strand distribution p-value = 7.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.10e-01

Only 100 of 124 total aligned reads displayed.

GCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCA  >  NC_000913/1764588‑1764784
                                                                                                   |                                                                                                   
 cATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTc                                                                                                    >  1:1352893/1‑100 (MQ=255)
    tAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTAAGAAATAAGAAAACCCTTAAGtat                                                                                                   >  1:2067804/1‑96 (MQ=255)
     aaGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCtgtg                                                                                                >  1:881399/1‑100 (MQ=255)
      aGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCtgtg                                                                                                <  1:854466/99‑1 (MQ=255)
      aGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGc                                                                                              <  2:1863664/100‑1 (MQ=255)
      aGGCAGTAATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGc                                                                                              <  1:2633467/100‑1 (MQ=255)
        gCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCg                                                                                             >  2:1258687/1‑99 (MQ=255)
         cTGTGGTGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAGATAAGAAAACCCTTAAGTCTGTGCGac                                                                                           >  1:1643707/1‑100 (MQ=255)
         cTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATGTAAGAGATAGGTAACAAAGTGAAGCGTTCAGAAATAAGAAAACGCTTAAGTCTGTGc                                                                                              >  1:616252/1‑97 (MQ=255)
              atgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCtgtgtcgcacaga                                                                                       <  2:257991/100‑10 (MQ=39)
               tgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGc                                                                                     >  2:1364973/1‑100 (MQ=255)
               tgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGc                                                                                     >  1:2492506/1‑100 (MQ=255)
                   aaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTaa                                                                                 <  2:2109487/100‑1 (MQ=255)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAgg                                                                               <  1:1032571/100‑1 (MQ=255)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAgg                                                                               >  2:1969671/1‑100 (MQ=255)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                               <  2:1219737/100‑1 (MQ=37)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                               <  1:1195683/100‑1 (MQ=37)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                               <  2:2168332/100‑1 (MQ=37)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                               <  1:2290791/100‑1 (MQ=37)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                              <  2:2772566/100‑1 (MQ=37)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                              <  1:668406/100‑1 (MQ=37)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                              <  2:1051797/100‑1 (MQ=37)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                              <  2:2385261/100‑1 (MQ=37)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGCTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTaa                                                                                 >  1:1760031/1‑97 (MQ=255)
                       aaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGt                                                                            >  1:129715/1‑100 (MQ=255)
                        aaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAGGGtt                                                                           <  2:800579/100‑1 (MQ=37)
                         aaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAGGGttt                                                                          <  2:681117/100‑1 (MQ=25)
                          aagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTc                                                                         <  1:547004/100‑1 (MQ=255)
                             ttGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAc                                                                      >  2:795163/1‑100 (MQ=255)
                              tGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcc                                                                     <  2:1163826/100‑1 (MQ=255)
                                tATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcccc                                                                   >  1:1227202/1‑100 (MQ=255)
                                tATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcccc                                                                   >  1:436299/1‑100 (MQ=255)
                                tATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAGGGttttcttatt                                                                   <  1:2275342/100‑8 (MQ=21)
                                 aTTGACAATGACTATTTAAAAATTAGGCGAAACAGTGCAGCGTTCAGAACTAATAAACCCCTTAGGTCTGTGCGCCACAGGCTTGCGGGTTGCTAcc                                                                     <  1:854122/97‑1 (MQ=255)
                                 aTTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCa                                                                  >  1:1567763/1‑100 (MQ=255)
                                 aTTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCa                                                                  >  1:2736947/1‑100 (MQ=255)
                                 aTTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCa                                                                  >  1:975718/1‑100 (MQ=255)
                                 aTTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCa                                                                  >  1:581334/1‑100 (MQ=255)
                                 aTTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCa                                                                  >  1:2219874/1‑100 (MQ=255)
                                  ttGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCAt                                                                 >  2:2782594/1‑100 (MQ=255)
                                  tagAAAATGACTAATTAAGAGATAGGTAAAAAAGTGCAGCGTACAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCAt                                                                 >  2:1976392/3‑100 (MQ=255)
                                   tGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAGGGttttcttatttct                                                                <  2:676990/100‑11 (MQ=18)
                                       aaTGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGgc                                                            <  2:581337/100‑1 (MQ=255)
                                          gACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCt                                                         <  1:204481/100‑1 (MQ=255)
                                           aCTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACACTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCtt                                                        >  1:2553567/1‑100 (MQ=255)
                                            cTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTa                                                       <  2:1300216/100‑1 (MQ=255)
                                            cTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTa                                                       <  2:1790275/100‑1 (MQ=255)
                                            cTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTa                                                       <  2:1923181/100‑1 (MQ=255)
                                            cTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTa                                                       <  1:1105637/100‑1 (MQ=255)
                                              aTTTGAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCTCGTAtc                                                     >  2:2188081/1‑100 (MQ=255)
                                              aTTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAtc                                                     >  2:39846/1‑100 (MQ=255)
                                              aTTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAtc                                                     >  1:539626/1‑100 (MQ=255)
                                                 tAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTcc                                                  >  1:2493418/1‑100 (MQ=255)
                                                 tAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTcc                                                  <  2:1673986/100‑1 (MQ=255)
                                                 tAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTcc                                                  >  1:1994092/1‑100 (MQ=255)
                                                 tAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTCCTACCCCATCCGGCGCTTATCTcc                                                  <  2:1808461/100‑1 (MQ=255)
                                                   agcgaTAGGTAAAAAAGTGCAGCGTTCAGAAATCAGAAAACCCTTAAGACTGTGCGACACAGGCATAAGGGTTTCTACCCCATCCGGCGCTTAtc                                                     >  1:2754515/4‑95 (MQ=255)
                                                      gaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCAc                                             >  2:1652584/1‑100 (MQ=255)
                                                         gatctaaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCActcn                                          <  2:1320636/95‑2 (MQ=255)
                                                          ggTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACtctc                                         >  1:2577993/1‑100 (MQ=255)
                                                           gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                        >  2:708711/1‑100 (MQ=255)
                                                           gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                        >  1:1495834/1‑100 (MQ=255)
                                                           gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                        >  2:1843674/1‑100 (MQ=255)
                                                           gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                        <  2:1120120/100‑1 (MQ=255)
                                                           gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                        <  2:106859/100‑1 (MQ=255)
                                                           gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGATTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                        >  2:834526/1‑100 (MQ=255)
                                                            tAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAg                                       >  1:1881937/1‑100 (MQ=255)
                                                              naaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCActctagatc                                     >  1:1320631/2‑95 (MQ=255)
                                                               aaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTgg                                    >  1:392365/1‑100 (MQ=255)
                                                                     gCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGc                              >  2:1547295/1‑100 (MQ=255)
                                                                          gTTCATAAATAAGAAAACCCTTAAGTCAGTGCGACAAAGGCTTAAGGTTTTCTACGCCATCCGGCGCTTATCTCCGTCACTCTCAGTTACTTAGCTCTTg                         <  1:2315138/100‑1 (MQ=255)
                                                                          attCAGAAATAAGAAAACACTTAAGTCTGTGCGACACAGGATTAAGGGATTCTACACCAAAGGGCGCTTAACTACGGCACTGTCAGTGGCTTAActgt                           >  1:1408935/2‑96 (MQ=255)
                                                                           ttCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGa                        >  1:1858204/1‑100 (MQ=255)
                                                                            tCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGaa                       <  2:1953559/100‑1 (MQ=255)
                                                                             cAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAg                      >  2:1557753/1‑100 (MQ=255)
                                                                             cAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAg                      >  2:462891/1‑100 (MQ=255)
                                                                             cAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAg                      >  1:2058803/1‑100 (MQ=255)
                                                                               gAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCTTCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAggg                    <  1:708688/100‑1 (MQ=255)
                                                                               gAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGctctagatcgg                    >  1:2899435/1‑93 (MQ=255)
                                                                                 aaTAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGc                  <  1:2382659/100‑1 (MQ=255)
                                                                                 aaTAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGc                  >  2:2356893/1‑100 (MQ=255)
                                                                                  ttccgatcccccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGc                  <  2:694502/91‑1 (MQ=21)
                                                                                     aGAAAACCCTGAAGTCTGTGCGACACAGGCTGAAGGGTTTCTACCCCATCCGGGGCATATGTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa              <  1:2801541/100‑1 (MQ=255)
                                                                                        aaaCCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGa           <  1:462888/100‑1 (MQ=255)
                                                                                         aaCCCTTGAGTCTGTGCGACACAGGCTTAAGGGTTTCGACCCCAGCCGGCGCTTATCGCCGGCACTCGCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaa          <  1:386191/100‑1 (MQ=255)
                                                                                         aaCCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaa          >  2:1163599/1‑100 (MQ=255)
                                                                                         aaCCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaa          <  2:1490620/100‑1 (MQ=255)
                                                                                         aaCCCTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaa          >  2:1635889/1‑100 (MQ=37)
                                                                                          aCCCTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCAGTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaat         >  2:2778896/1‑100 (MQ=25)
                                                                                          aCCCTTAAGCCTGTGTCGCACAGACTTAAGGGTTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaa          >  2:573577/1‑100 (MQ=25)
                                                                                           cccTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaata        <  2:1352898/100‑1 (MQ=255)
                                                                                           cccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaata        >  2:1801245/1‑100 (MQ=37)
                                                                                           cccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGcagatcgga          >  1:694499/1‑91 (MQ=18)
                                                                                           cccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaat         >  1:824994/1‑99 (MQ=37)
                                                                                            ccTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataa       >  2:2077286/1‑100 (MQ=255)
                                                                                            ccTTAAGCATGTGCGACACAGGCTCAAGGGTTTCTACCCCATCCGGCGCTTATCTCCTTCACGCTCATTGTCTTAGCTCTGGAAGTGTCGATAAGaata        >  1:1261223/1‑99 (MQ=255)
                                                                                             cTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataa       <  1:1955624/99‑1 (MQ=255)
                                                                                               tAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtct    <  2:1898033/100‑1 (MQ=255)
                                                                                               tAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtct    >  2:193411/1‑100 (MQ=255)
                                                                                                 aGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCa  <  2:359337/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCA  >  NC_000913/1764588‑1764784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: