Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,861,419 A→G 19.8% intergenic (‑87/+283) ydjL ← / ← yeaC putative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,861,4190AG19.8% 256.1 / 58.7 126intergenic (‑87/+283)ydjL/yeaCputative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base A (45/56);  new base G (14/11);  total (59/67)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 130 total aligned reads displayed.

AGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAA  >  NC_000913/1861322‑1861517
                                                                                                 |                                                                                                  
aGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAAcaca                                                                                                  <  1:1091658/100‑1 (MQ=255)
 gTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAAcaca                                                                                                  <  1:1838790/99‑1 (MQ=255)
  tGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGAGGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAgn                                                                                                <  1:809337/100‑2 (MQ=255)
     tttCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATaa                                                                                             <  2:298430/100‑1 (MQ=255)
      ttCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAAt                                                                                            <  1:2770716/100‑1 (MQ=255)
       tCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATg                                                                                           >  2:2910237/1‑100 (MQ=255)
        cATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCAtta                                                                                          <  2:2166478/100‑3 (MQ=255)
         aTTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGAt                                                                                         >  1:1089909/1‑100 (MQ=255)
              gTCATCTTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCTCATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCg                                                                                    <  1:2029063/100‑1 (MQ=255)
              gTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCg                                                                                    <  1:532680/100‑1 (MQ=255)
                  tcttctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                <  1:1903403/97‑1 (MQ=255)
                  tactctGTGTTTAGTTTTTCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAATCACAGATAATGATCTTCGtttt                                                                                <  1:1012653/98‑1 (MQ=255)
                  tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGtttt                                                                                <  2:624872/100‑1 (MQ=38)
                  tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                <  1:2452678/100‑1 (MQ=255)
                  tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                <  1:2016/100‑1 (MQ=255)
                  tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                >  2:33867/1‑100 (MQ=255)
                    ntctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                              >  1:1398620/2‑100 (MQ=255)
                    ctctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                              >  1:1484905/1‑100 (MQ=255)
                    ctctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                              <  1:1705006/100‑1 (MQ=255)
                    ctctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                              <  2:910934/100‑1 (MQ=255)
                     tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACa                                                                             <  2:1235752/100‑1 (MQ=38)
                      ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTTCCCACATGAATATTGAGGTAAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACag                                                                            <  2:317333/100‑2 (MQ=25)
                      ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACag                                                                            <  2:108847/100‑2 (MQ=37)
                      ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACaa                                                                            <  1:355078/100‑1 (MQ=255)
                       tgtgTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTATATCTGTAAAACACAGATAATGATCTGCGTTTTACAAc                                                                           <  1:1560131/100‑1 (MQ=255)
                        gtgtTTAGTTTTGCTTAGATCGCCATCATACTTACCAACATGATTTGTGGGTTCGACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAAc                                                                           >  1:966043/1‑99 (MQ=255)
                         tgtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTc                                                                         <  1:511223/100‑1 (MQ=255)
                          gtTTAGTTTTGCTTAGTTGGCCATCATACTTTCCCAAAGTAATTTTCAGCTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTc                                                                         <  1:1496891/99‑1 (MQ=255)
                          gtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCa                                                                        >  2:1625194/1‑100 (MQ=255)
                          gtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCa                                                                        >  1:1259400/1‑100 (MQ=255)
                             tAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGAt                                                                     >  1:2213842/1‑100 (MQ=255)
                               gTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATca                                                                   >  2:2666588/1‑100 (MQ=255)
                                ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATcac                                                                  <  2:2105839/100‑1 (MQ=255)
                                 tttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATcaca                                                                 >  1:2409677/1‑100 (MQ=255)
                                 tttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATcaca                                                                 <  1:2669929/100‑1 (MQ=255)
                                   tGCTTAGATGGCCATCATACTTACCCACATTATTTTTTAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACaan                                                               <  1:2012185/100‑2 (MQ=255)
                                    gCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcattatc                                                              <  2:1559581/100‑6 (MQ=37)
                                    gCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcattatc                                                              <  2:608822/100‑6 (MQ=37)
                                     cTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTa                                                             >  1:1144875/1‑100 (MQ=255)
                                     cTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTa                                                             <  1:976739/100‑1 (MQ=255)
                                      ttAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcattatctg                                                            <  1:949627/100‑8 (MQ=25)
                                       tAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACg                                                           >  1:2688459/1‑100 (MQ=255)
                                         gATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCa                                                         >  1:301627/1‑100 (MQ=255)
                                          aTGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAg                                                        <  1:2155586/100‑1 (MQ=255)
                                             gCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaaa                                                     >  2:1698680/1‑100 (MQ=255)
                                                 tcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATgg                                                 <  2:836528/100‑1 (MQ=255)
                                                  catACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGc                                                <  2:1452739/100‑1 (MQ=255)
                                                   atACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCt                                               <  2:2213855/100‑1 (MQ=255)
                                                      cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                            <  2:1474146/100‑1 (MQ=255)
                                                      cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                            <  2:2324075/100‑1 (MQ=255)
                                                      cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                            <  2:694342/100‑1 (MQ=255)
                                                      cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                            >  1:1013807/1‑100 (MQ=255)
                                                      cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                            <  2:1259405/100‑1 (MQ=255)
                                                          cccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTg                                        >  2:77885/1‑100 (MQ=255)
                                                           ccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGt                                       >  1:2135891/1‑100 (MQ=255)
                                                            cacaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGtt                                      >  2:2203599/1‑100 (MQ=255)
                                                            cacaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGt                                       >  1:2781960/1‑99 (MQ=255)
                                                             acaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTa                                     >  1:2569884/1‑100 (MQ=255)
                                                             acaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACACAGAAAATGGCTGGGTCTGTTa                                     >  2:2104641/1‑100 (MQ=255)
                                                               aTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACa                                   <  1:2201540/100‑1 (MQ=255)
                                                                 gATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCGGGGTCTGTTACAgg                                 >  2:2330468/1‑100 (MQ=255)
                                                                  aTTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                <  2:1013810/100‑1 (MQ=255)
                                                                   tttttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGtt                               <  1:1375127/100‑1 (MQ=255)
                                                                    ttttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGtt                               <  2:1290809/99‑1 (MQ=255)
                                                                    ttttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTg                              <  1:2160577/100‑1 (MQ=255)
                                                                      ttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                            >  1:1061456/1‑100 (MQ=255)
                                                                       tGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                           <  1:101675/100‑1 (MQ=255)
                                                                       tGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                           <  1:2898091/100‑1 (MQ=255)
                                                                        gAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATgg                          <  2:2562411/100‑1 (MQ=255)
                                                                          ggTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGa                         >  1:2514320/1‑99 (MQ=255)
                                                                             cAACATTAAATCTGTAAAACACAGATAATGATATGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAg                       <  1:2238542/98‑1 (MQ=255)
                                                                                cATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCggg                  <  1:2815538/100‑1 (MQ=255)
                                                                                 aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCgggg                 >  1:1251924/1‑100 (MQ=255)
                                                                                  ttAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTAAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgc                >  1:1442228/1‑100 (MQ=255)
                                                                                     gagttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCa             >  2:276908/5‑100 (MQ=37)
                                                                                     aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCa             >  2:16478/1‑100 (MQ=255)
                                                                                     aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCa             >  1:556748/1‑100 (MQ=255)
                                                                                       gttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCGGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaa           >  2:1840337/3‑100 (MQ=25)
                                                                                       gttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaa           >  2:1771765/3‑100 (MQ=37)
                                                                                         tttAAAACGCAGATCATTACCTGTGTTTTACAACTCAGATCACAATTAAGCAGAAAATGGCTGGGTCTGTTACAAGTTCATGGAAGGCGGGGCACaaaaa         >  1:789212/3‑100 (MQ=25)
                                                                                         tGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaaa         >  2:2003496/1‑100 (MQ=38)
                                                                                         tGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaa          <  1:1086022/99‑1 (MQ=255)
                                                                                          gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGTCTGGGTCTGTTACAGGTTGATGGAAGGCGTGGCGCAAAAag        >  1:327737/1‑100 (MQ=37)
                                                                                          gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag        >  2:388106/1‑100 (MQ=38)
                                                                                          gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACACAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag        >  2:1714521/1‑100 (MQ=37)
                                                                                          gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag        >  1:2085086/1‑100 (MQ=255)
                                                                                          gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag        <  2:1149846/100‑1 (MQ=255)
                                                                                          gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag        <  1:1347587/100‑1 (MQ=255)
                                                                                          gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag        <  2:1845087/100‑1 (MQ=255)
                                                                                          gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag        <  1:2743793/100‑1 (MQ=255)
                                                                                           tAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga       <  2:1874895/100‑1 (MQ=255)
                                                                                            aaaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag      >  2:1991002/1‑100 (MQ=38)
                                                                                            aaaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag      >  2:1751987/1‑100 (MQ=38)
                                                                                            aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag      <  2:2688475/100‑1 (MQ=255)
                                                                                             aaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAACATGTCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc     >  1:99653/1‑100 (MQ=38)
                                                                                             aaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc     >  2:1817115/1‑100 (MQ=38)
                                                                                             aaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc     >  2:1355339/1‑100 (MQ=38)
                                                                                             aaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc     >  2:2783689/1‑100 (MQ=255)
                                                                                             aaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc     >  2:2002497/1‑100 (MQ=255)
                                                                                                cacaGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaaa  <  2:1287998/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
AGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAA  >  NC_000913/1861322‑1861517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: