Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,861,425 A→C 19.3% intergenic (‑93/+277) ydjL ← / ← yeaC putative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,861,4250AC19.3% 296.8 / 69.6 128intergenic (‑93/+277)ydjL/yeaCputative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base A (46/57);  new base C (14/11);  total (60/68)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGA  >  NC_000913/1861327‑1861524
                                                                                                  |                                                                                                   
tttCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATaa                                                                                                    <  2:298430/100‑1 (MQ=255)
  tCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATg                                                                                                  >  2:2910237/1‑100 (MQ=255)
   cATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACGTTAAATCTGTAAAACACAGATAATGa                                                                                                 <  2:1774229/100‑1 (MQ=255)
   cATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCAtta                                                                                                 <  2:2166478/100‑3 (MQ=255)
    aTTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGAt                                                                                                >  1:1089909/1‑100 (MQ=255)
         gTCATCTTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCTCATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCg                                                                                           <  1:2029063/100‑1 (MQ=255)
         gTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCg                                                                                           <  1:532680/100‑1 (MQ=255)
          tCATCCTCTTTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTAAACATTAAATCTGTAAAACACAGATAATGATCTGCGt                                                                                          <  1:2111640/100‑1 (MQ=255)
            aTCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGttt                                                                                        <  2:1081202/100‑1 (MQ=38)
             tcttctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                       <  1:1903403/97‑1 (MQ=255)
             tactctGTGTTTAGTTTTTCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAATCACAGATAATGATCTTCGtttt                                                                                       <  1:1012653/98‑1 (MQ=255)
             tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                       >  2:33867/1‑100 (MQ=255)
              ccTCTGTTTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCTTTTTa                                                                                      >  1:1287993/1‑100 (MQ=255)
               ntctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                                     >  1:1398620/2‑100 (MQ=255)
               ctctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                                     >  1:1484905/1‑100 (MQ=255)
               ctctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                                     <  2:910934/100‑1 (MQ=255)
                tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACa                                                                                    <  1:2357428/100‑1 (MQ=38)
                tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACa                                                                                    <  2:1235752/100‑1 (MQ=38)
                 ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTTCCCACATGAATATTGAGGTAAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACag                                                                                   <  2:317333/100‑2 (MQ=25)
                 ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACaa                                                                                   <  1:355078/100‑1 (MQ=255)
                  tgtgTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTATATCTGTAAAACACAGATAATGATCTGCGTTTTACAAc                                                                                  <  1:1560131/100‑1 (MQ=255)
                   gtgtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACagat                                                                                 <  1:1661360/100‑4 (MQ=37)
                   gtgtTTAGTTTTGCTTAGATCGCCATCATACTTACCAACATGATTTGTGGGTTCGACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAAc                                                                                  >  1:966043/1‑99 (MQ=255)
                    tgtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACagatt                                                                                <  2:2099708/100‑5 (MQ=25)
                    tgtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTc                                                                                <  1:511223/100‑1 (MQ=255)
                    tgtTTAGTGTTGCTTAGATGGCCATCATACTTCTCCACATGATTTTCGAGTTGAACATTAACGCTGTAAAGCGCGGACCATTATCTGTGTTTTACagat                                                                                 <  1:2189966/99‑4 (MQ=16)
                     gtTTAGTTTTGCTTAGTTGGCCATCATACTTTCCCAAAGTAATTTTCAGCTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTc                                                                                <  1:1496891/99‑1 (MQ=255)
                     gtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCa                                                                               >  1:1259400/1‑100 (MQ=255)
                        tAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGAt                                                                            >  1:2213842/1‑100 (MQ=255)
                          gTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATca                                                                          >  2:2666588/1‑100 (MQ=255)
                           ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATcac                                                                         <  2:2105839/100‑1 (MQ=255)
                            tttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATcaca                                                                        >  1:2409677/1‑100 (MQ=255)
                              tGCTTAGATGGCCATCATACTTACCCACATTATTTTTTAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACaan                                                                      <  1:2012185/100‑2 (MQ=255)
                               gCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcattatc                                                                     <  2:1559581/100‑6 (MQ=37)
                               gCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcattatc                                                                     <  2:608822/100‑6 (MQ=37)
                                 ttAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcattatctg                                                                   <  1:949627/100‑8 (MQ=25)
                                  tAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACg                                                                  >  1:2688459/1‑100 (MQ=255)
                                    gATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCa                                                                >  1:301627/1‑100 (MQ=255)
                                     aTGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAg                                                               <  1:2155586/100‑1 (MQ=255)
                                        gCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaaa                                                            >  2:1698680/1‑100 (MQ=255)
                                            tcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATgg                                                        <  2:1612829/100‑1 (MQ=255)
                                              atACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCt                                                      <  2:2213855/100‑1 (MQ=255)
                                                 cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                   <  2:1474146/100‑1 (MQ=255)
                                                 cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                   >  2:2692505/1‑100 (MQ=255)
                                                 cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                   <  2:2324075/100‑1 (MQ=255)
                                                 cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                   <  2:694342/100‑1 (MQ=255)
                                                 cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                   >  1:1111195/1‑100 (MQ=255)
                                                 cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                   >  1:1013807/1‑100 (MQ=255)
                                                     cccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTg                                               >  2:749330/1‑100 (MQ=255)
                                                     cccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTg                                               >  2:77885/1‑100 (MQ=255)
                                                       cacaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGt                                              >  1:2781960/1‑99 (MQ=255)
                                                        acaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTa                                            >  1:2569884/1‑100 (MQ=255)
                                                         caTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTAc                                           >  1:2887909/1‑100 (MQ=255)
                                                            gATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCGGGGTCTGTTACAgg                                        >  2:2330468/1‑100 (MQ=255)
                                                             aTTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                       <  2:1013810/100‑1 (MQ=255)
                                                              tttttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGtt                                      <  1:1375127/100‑1 (MQ=255)
                                                               ttttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGtt                                      <  2:1290809/99‑1 (MQ=255)
                                                               ttttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTg                                     <  1:2160577/100‑1 (MQ=255)
                                                                 ttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                   >  1:1061456/1‑100 (MQ=255)
                                                                  tGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                  <  1:101675/100‑1 (MQ=255)
                                                                   gAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATgg                                 <  2:2562411/100‑1 (MQ=255)
                                                                     ggTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGa                                >  1:2514320/1‑99 (MQ=255)
                                                                      gTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAg                              >  2:134157/1‑100 (MQ=255)
                                                                       tCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAgg                             >  1:2664392/1‑100 (MQ=255)
                                                                        cAACATTAAATCTGTAAAACACAGATAATGATATGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAg                              <  1:2238542/98‑1 (MQ=255)
                                                                         aaCATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCg                           >  2:2193559/1‑100 (MQ=255)
                                                                           cATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCggg                         <  1:2815538/100‑1 (MQ=255)
                                                                            aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCgggg                        >  1:1251924/1‑100 (MQ=255)
                                                                             ttAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTAAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgc                       >  1:1442228/1‑100 (MQ=255)
                                                                              tAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgcg                      <  2:1252145/100‑1 (MQ=255)
                                                                                gagttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCa                    >  2:276908/5‑100 (MQ=37)
                                                                                aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCa                    >  2:16478/1‑100 (MQ=255)
                                                                                aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCa                    >  1:556748/1‑100 (MQ=255)
                                                                                  gttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCGGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaa                  >  2:1840337/3‑100 (MQ=25)
                                                                                  gttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaa                  >  2:1771765/3‑100 (MQ=37)
                                                                                   cTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaa                 >  1:2764363/1‑100 (MQ=255)
                                                                                   cTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGCTGATGGAAGGCGGGGCGCacaa                 >  1:961761/1‑100 (MQ=255)
                                                                                    tGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaaa                >  2:2003496/1‑100 (MQ=38)
                                                                                    tGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaa                 <  1:1086022/99‑1 (MQ=255)
                                                                                     gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGTCTGGGTCTGTTACAGGTTGATGGAAGGCGTGGCGCAAAAag               >  1:327737/1‑100 (MQ=37)
                                                                                     gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACACAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag               >  2:1713720/1‑100 (MQ=37)
                                                                                     gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag               >  1:2085086/1‑100 (MQ=255)
                                                                                     gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag               <  2:1149846/100‑1 (MQ=255)
                                                                                     gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag               <  1:2743793/100‑1 (MQ=255)
                                                                                     gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag               <  2:1845087/100‑1 (MQ=255)
                                                                                      tAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga              <  2:1874895/100‑1 (MQ=255)
                                                                                       aaaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag             >  2:1751987/1‑100 (MQ=38)
                                                                                       aaaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag             >  2:1991002/1‑100 (MQ=38)
                                                                                       aaaaCACAGATAATGAGCTGCGTTTTACAACGCAGATCACAATTACGCAGAAAATGGCTGAGTCTGTTACAGGTTGATGGAAGGAGAGGAGCAAAAagag             <  1:1970/100‑1 (MQ=255)
                                                                                        aaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAACATGTCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc            >  1:99653/1‑100 (MQ=38)
                                                                                        aaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc            >  2:1355339/1‑100 (MQ=38)
                                                                                        aaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc            >  2:2783689/1‑100 (MQ=255)
                                                                                        aaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc            >  2:2002497/1‑100 (MQ=255)
                                                                                         aaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCa           <  2:946943/100‑1 (MQ=255)
                                                                                         aaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCa           >  1:736538/1‑100 (MQ=255)
                                                                                           cacaGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaaa         <  2:1287998/100‑1 (MQ=255)
                                                                                               gATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCg     <  2:553298/100‑1 (MQ=255)
                                                                                                aTAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCga    <  2:871149/100‑1 (MQ=255)
                                                                                                 tAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCgag   >  2:401990/1‑100 (MQ=255)
                                                                                                  aaTGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCgaga  <  2:255513/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
TTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGA  >  NC_000913/1861327‑1861524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: