Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,861,428 G→T 17.9% intergenic (‑96/+274) ydjL ← / ← yeaC putative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,861,4280GT17.9% 339.7 / 58.4 132intergenic (‑96/+274)ydjL/yeaCputative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base G (47/61);  new base T (14/10);  total (61/71)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

Only 100 of 136 total aligned reads displayed.

TCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGG  >  NC_000913/1861329‑1861527
                                                                                                   |                                                                                                   
tCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATg                                                                                                     >  2:2910237/1‑100 (MQ=255)
 cATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACGTTAAATCTGTAAAACACAGATAATGa                                                                                                    <  2:1774229/100‑1 (MQ=255)
       gTCATCTTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCTCATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCg                                                                                              <  1:2029063/100‑1 (MQ=255)
       gTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCg                                                                                              <  1:532680/100‑1 (MQ=255)
        tCATCCTCTTTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTAAACATTAAATCTGTAAAACACAGATAATGATCTGCGt                                                                                             <  1:2111640/100‑1 (MQ=255)
          aTCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGttt                                                                                           <  2:1081202/100‑1 (MQ=38)
           tcttctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                          <  1:1903403/97‑1 (MQ=255)
           tactctGTGTTTAGTTTTTCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAATCACAGATAATGATCTTCGtttt                                                                                          <  1:1012653/98‑1 (MQ=255)
           tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGtttt                                                                                          <  2:624872/100‑1 (MQ=38)
           tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                          >  2:33867/1‑100 (MQ=255)
           tCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                          <  1:2016/100‑1 (MQ=255)
            ccTCTGTTTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCTTTTTa                                                                                         >  1:1287993/1‑100 (MQ=255)
             ctctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                                        >  1:1484905/1‑100 (MQ=255)
             ctctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                                        <  2:910934/100‑1 (MQ=255)
             ctctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                                        <  1:1705006/100‑1 (MQ=255)
              tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACa                                                                                       <  2:1235752/100‑1 (MQ=38)
              tctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACa                                                                                       <  1:2357428/100‑1 (MQ=38)
               ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTTCCCACATGAATATTGAGGTAAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACag                                                                                      <  2:317333/100‑2 (MQ=25)
               ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACag                                                                                      <  1:1063225/100‑2 (MQ=37)
               ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACaa                                                                                      <  1:355078/100‑1 (MQ=255)
                tgtgTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTATATCTGTAAAACACAGATAATGATCTGCGTTTTACAAc                                                                                     <  1:1560131/100‑1 (MQ=255)
                 gtgtTTAGTTTTGCTTAGATCGCCATCATACTTACCAACATGATTTGTGGGTTCGACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAAc                                                                                     >  1:966043/1‑99 (MQ=255)
                  tgtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACagatt                                                                                   <  2:2099708/100‑5 (MQ=25)
                  tgtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTc                                                                                   <  1:511223/100‑1 (MQ=255)
                  tgtTTAGTGTTGCTTAGATGGCCATCATACTTCTCCACATGATTTTCGAGTTGAACATTAACGCTGTAAAGCGCGGACCATTATCTGTGTTTTACagat                                                                                    <  1:2189966/99‑4 (MQ=16)
                   gtTTAGTTTTGCTTAGTTGGCCATCATACTTTCCCAAAGTAATTTTCAGCTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTc                                                                                   <  1:1496891/99‑1 (MQ=255)
                        gTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATca                                                                             >  2:2666588/1‑100 (MQ=255)
                         ttttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATcac                                                                            <  2:2105839/100‑1 (MQ=255)
                          tttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATcaca                                                                           >  1:2409677/1‑100 (MQ=255)
                            tGCTTAGATGGCCATCATACTTACCCACATTATTTTTTAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACaan                                                                         <  1:2012185/100‑2 (MQ=255)
                             gCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcattatc                                                                        <  2:1559581/100‑6 (MQ=37)
                             gCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcattatc                                                                        <  2:608822/100‑6 (MQ=37)
                              cTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTa                                                                       <  1:976739/100‑1 (MQ=255)
                              cTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTa                                                                       >  1:1144875/1‑100 (MQ=255)
                               ttAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcattatctg                                                                      <  1:949627/100‑8 (MQ=25)
                                tAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACg                                                                     >  1:2688459/1‑100 (MQ=255)
                                  gATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCa                                                                   >  1:301627/1‑100 (MQ=255)
                                      gCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaaa                                                               >  2:1698680/1‑100 (MQ=255)
                                          tcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATgg                                                           <  2:836528/100‑1 (MQ=255)
                                          tcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATgg                                                           <  2:1612829/100‑1 (MQ=255)
                                           catACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGc                                                          <  2:1452739/100‑1 (MQ=255)
                                            atACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCt                                                         <  2:2213855/100‑1 (MQ=255)
                                             tACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTg                                                        <  2:2461891/100‑1 (MQ=255)
                                               cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                      <  2:1259405/100‑1 (MQ=255)
                                               cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                      <  2:2324075/100‑1 (MQ=255)
                                               cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                      >  2:2692505/1‑100 (MQ=255)
                                               cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                      >  1:1111195/1‑100 (MQ=255)
                                               cTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggg                                                      >  1:1013807/1‑100 (MQ=255)
                                                   cccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTg                                                  >  2:749330/1‑100 (MQ=255)
                                                   cccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTg                                                  >  2:77885/1‑100 (MQ=255)
                                                    ccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGt                                                 >  1:2135891/1‑100 (MQ=255)
                                                     cacaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGtt                                                >  2:2203599/1‑100 (MQ=255)
                                                      acaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTa                                               >  1:2569884/1‑100 (MQ=255)
                                                      acaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACACAGAAAATGGCTGGGTCTGTTa                                               >  2:2104641/1‑100 (MQ=255)
                                                       caTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTAc                                              >  1:2887909/1‑100 (MQ=255)
                                                        aTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACa                                             <  1:2201540/100‑1 (MQ=255)
                                                           aTTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                          <  2:1013810/100‑1 (MQ=255)
                                                            tttttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGtt                                         <  1:1375127/100‑1 (MQ=255)
                                                             ttttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTg                                        <  1:2160577/100‑1 (MQ=255)
                                                               ttGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                      >  1:1061456/1‑100 (MQ=255)
                                                                tGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATg                                     <  1:101675/100‑1 (MQ=255)
                                                                 gAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATgg                                    <  2:2562411/100‑1 (MQ=255)
                                                                   ggTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGa                                   >  1:2514320/1‑99 (MQ=255)
                                                                    gTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAg                                 >  2:134157/1‑100 (MQ=255)
                                                                     tCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAgg                                >  1:2664392/1‑100 (MQ=255)
                                                                      cAACATTAAATCTGTAAAACACAGATAATGATATGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAg                                 <  1:2238542/98‑1 (MQ=255)
                                                                         cATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCggg                            <  1:2815538/100‑1 (MQ=255)
                                                                          aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCgggg                           >  1:1251924/1‑100 (MQ=255)
                                                                           ttAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTAAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgc                          >  1:1442228/1‑100 (MQ=255)
                                                                              aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCa                       >  2:16478/1‑100 (MQ=255)
                                                                              aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCa                       >  1:556748/1‑100 (MQ=255)
                                                                                gttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCGGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaa                     >  2:1840337/3‑100 (MQ=25)
                                                                                gttGTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaa                     >  2:1771765/3‑100 (MQ=37)
                                                                                 cTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaa                    >  1:2764363/1‑100 (MQ=255)
                                                                                  tttAAAACGCAGATCATTACCTGTGTTTTACAACTCAGATCACAATTAAGCAGAAAATGGCTGGGTCTGTTACAAGTTCATGGAAGGCGGGGCACaaaaa                   >  1:789212/3‑100 (MQ=25)
                                                                                  tGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaa                    <  1:1086022/99‑1 (MQ=255)
                                                                                   gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGTCTGGGTCTGTTACAGGTTGATGGAAGGCGTGGCGCAAAAag                  >  1:327737/1‑100 (MQ=37)
                                                                                   gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag                  >  2:388106/1‑100 (MQ=38)
                                                                                   gTAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACACAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag                  >  2:1713720/1‑100 (MQ=37)
                                                                                   gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag                  <  1:2743793/100‑1 (MQ=255)
                                                                                   gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag                  <  1:1347587/100‑1 (MQ=255)
                                                                                   gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag                  <  2:1845087/100‑1 (MQ=255)
                                                                                   gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAag                  <  2:1149846/100‑1 (MQ=255)
                                                                                    tAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                 <  2:1874895/100‑1 (MQ=255)
                                                                                     aaaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag                >  2:1991002/1‑100 (MQ=38)
                                                                                     aaaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag                >  2:1751987/1‑100 (MQ=38)
                                                                                     aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAagag                <  2:2688475/100‑1 (MQ=255)
                                                                                      aaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc               >  2:1355339/1‑100 (MQ=38)
                                                                                      aaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc               >  2:2002497/1‑100 (MQ=255)
                                                                                      aaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGc               >  2:2783689/1‑100 (MQ=255)
                                                                                       aaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCa              <  2:946943/100‑1 (MQ=255)
                                                                                         gcgtaattgtGATCTGAGTTGTAAAACGCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaa             >  1:888488/10‑99 (MQ=25)
                                                                                         cacaGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCaaa            <  2:1287998/100‑1 (MQ=255)
                                                                                             gATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCg        <  2:553298/100‑1 (MQ=255)
                                                                                              tttgtGATCTGAGTTGTAAAACGCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCga       <  2:101894/96‑1 (MQ=37)
                                                                                                aaTGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCgaga     <  2:255513/100‑1 (MQ=255)
                                                                                                 aTGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGagag    <  2:1258048/100‑1 (MQ=255)
                                                                                                   gATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCATAAAGAGCAAATTCGAGAggg  <  1:1781671/100‑1 (MQ=255)
                                                                                                   gATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAggg  <  2:1130973/100‑1 (MQ=255)
                                                                                                   gATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAAGTTGAGAgg   >  1:1407775/1‑99 (MQ=255)
                                                                                                   |                                                                                                   
TCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGG  >  NC_000913/1861329‑1861527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: