Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,122,191 (T)5→4 coding (97/531 nt) hslV ← peptidase component of the HslUV protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,122,1870T.98.2% 490.4 / 1.1 112coding (101/531 nt)hslVpeptidase component of the HslUV protease
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base . (48/62);  total (48/64)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 126 total aligned reads displayed.

ATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTT  >  NC_000913/4122088‑4122282
                                                                                                   |                                                                                               
aTTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGTGGACCt                                                                                                 >  2:2045132/1‑100 (MQ=255)
aTTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCt                                                                                                 >  2:735075/1‑100 (MQ=255)
aTTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCt                                                                                                 >  2:1796314/1‑100 (MQ=255)
aTTTCCAGTTTACGTTCAAACAGTTCGAACACCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCt                                                                                                 <  1:1095236/100‑1 (MQ=255)
  ttCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCttt                                                                                               <  2:924230/100‑1 (MQ=255)
  ttCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCttt                                                                                               >  2:2356484/1‑100 (MQ=255)
   tCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCtttt                                                                                              <  2:87774/100‑1 (MQ=255)
   tCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCtttt                                                                                              >  1:2777872/1‑100 (MQ=255)
    ccAGTTTACGTTCAAACAGTTCGAACAGCGTAAATGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCttttc                                                                                             <  2:1599223/100‑2 (MQ=255)
     cAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCttttca                                                                                            >  1:843189/1‑98 (MQ=255)
     cAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCttttca                                                                                            <  1:1753985/100‑3 (MQ=255)
      aGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCttttcac                                                                                           <  2:358523/100‑4 (MQ=255)
         ttACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGtt                                                                                       <  2:107507/100‑1 (MQ=255)
         ttACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGtt                                                                                       >  1:1650797/1‑100 (MQ=255)
         cgctgTTCGAACTGTTTGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGtt                                                                                       >  2:2783825/5‑100 (MQ=255)
          tACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTg                                                                                      >  1:1062973/1‑100 (MQ=255)
          tACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTg                                                                                      <  2:1784881/100‑1 (MQ=255)
          gctgTTCGAACTGTTTGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTg                                                                                      >  1:2787965/4‑100 (MQ=255)
           aCGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGc                                                                                     <  1:686659/100‑1 (MQ=255)
           aCGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCAATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGc                                                                                     >  2:1373720/1‑100 (MQ=255)
            cGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGcc                                                                                    >  2:2240735/1‑100 (MQ=255)
            cGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGcc                                                                                    <  1:1861080/100‑1 (MQ=255)
            cGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGcc                                                                                    <  2:347289/100‑1 (MQ=255)
             gTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCTGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCt                                                                                   <  1:2557760/100‑1 (MQ=255)
             gTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCt                                                                                   <  1:1116333/100‑1 (MQ=255)
              ttCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCtt                                                                                  >  2:2996888/1‑100 (MQ=255)
               tCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCttt                                                                                 <  1:24569/100‑1 (MQ=255)
                cAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTc                                                                                >  2:1950146/1‑100 (MQ=255)
                 aaaCAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCa                                                                               >  1:2538659/1‑100 (MQ=255)
                    cAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTa                                                                            <  1:2697302/100‑1 (MQ=255)
                      gTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACg                                                                          >  1:1156074/1‑100 (MQ=255)
                       ttCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACgg                                                                         <  2:719544/100‑1 (MQ=255)
                        tCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGt                                                                        <  2:906539/100‑1 (MQ=255)
                         cGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTa                                                                       >  2:585469/1‑100 (MQ=255)
                          gAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTAt                                                                      >  2:1085292/1‑100 (MQ=255)
                             cAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGc                                                                   <  1:200864/100‑1 (MQ=255)
                             cAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGc                                                                   >  2:684746/1‑100 (MQ=255)
                              aGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGcc                                                                  >  1:176702/1‑100 (MQ=255)
                              aGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGcc                                                                  <  1:305001/100‑1 (MQ=255)
                               gCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGccc                                                                 >  2:629751/1‑100 (MQ=255)
                                cGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTACGGTATTGccc                                                                 <  1:2754460/100‑1 (MQ=255)
                                    naaGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGt                                                            >  2:1878738/2‑100 (MQ=255)
                                     aaGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTg                                                           <  2:2306025/100‑1 (MQ=255)
                                     aaGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTg                                                           <  2:2291450/100‑1 (MQ=255)
                                      aGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTgg                                                          >  2:209409/1‑100 (MQ=255)
                                      aGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTgg                                                          <  1:265931/100‑1 (MQ=255)
                                      aGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTgg                                                          <  1:1608627/100‑1 (MQ=255)
                                      aGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTgg                                                          >  2:1922730/1‑100 (MQ=255)
                                       gCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGc                                                         <  2:1983955/100‑1 (MQ=255)
                                        cATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGcn                                                        <  2:1488001/100‑2 (MQ=255)
                                         aTCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCt                                                       <  2:439919/100‑1 (MQ=255)
                                             gCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGAcc                                                   <  1:585476/100‑1 (MQ=255)
                                             gCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGAcc                                                   >  2:1575485/1‑100 (MQ=255)
                                              cAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCa                                                  <  1:361172/100‑1 (MQ=255)
                                                   ccgcccgcAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCAcc                                             <  1:665516/100‑1 (MQ=255)
                                                    cgcccgcAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCa                                            >  1:2567489/1‑100 (MQ=255)
                                                     gcccgcAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAg                                           <  1:209412/100‑1 (MQ=255)
                                                      cccgcAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGc                                          <  1:1512280/100‑1 (MQ=255)
                                                        cgcAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTGTCACGTTGCCTTTCATTACGGGATTGCCCAACGTGGCCTGACCATCACCAGCGa                                        >  2:2418656/1‑100 (MQ=255)
                                                         gcAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGAt                                       >  1:1269328/1‑100 (MQ=255)
                                                          cAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATg                                      <  1:735082/100‑1 (MQ=255)
                                                          cAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATg                                      <  2:1496004/100‑1 (MQ=255)
                                                           aaaGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGa                                     <  1:397425/100‑1 (MQ=255)
                                                            aaGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGAc                                    <  1:2216998/100‑1 (MQ=255)
                                                             aGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGAcc                                   <  2:1650783/100‑1 (MQ=255)
                                                              gCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACca                                  <  2:2394990/100‑1 (MQ=255)
                                                                ccGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACcaca                                <  2:2012661/100‑1 (MQ=255)
                                                                ccGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACcaca                                <  2:1282715/100‑1 (MQ=255)
                                                                 cgcgATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACAt                               <  1:677901/100‑1 (MQ=255)
                                                                  gcgATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATg                              <  1:2406141/100‑1 (MQ=255)
                                                                   cgATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATgg                             >  2:1752386/1‑100 (MQ=255)
                                                                    gATGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGc                            <  1:2688980/100‑1 (MQ=255)
                                                                     aTGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGGGATGACCACATGGcc                           <  2:478467/100‑1 (MQ=255)
                                                                      taaCTTTGTCGTGGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGc                            <  2:324208/96‑1 (MQ=255)
                                                                      tGACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCg                          <  1:2356505/100‑1 (MQ=255)
                                                                       gACTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGt                         >  2:2664981/1‑100 (MQ=255)
                                                                         cTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTa                       >  2:1484173/1‑100 (MQ=255)
                                                                         cTTTGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTa                       >  2:339601/1‑100 (MQ=255)
                                                                            tGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACgg                    <  2:1244335/100‑1 (MQ=255)
                                                                            tGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACgg                    <  1:203183/100‑1 (MQ=255)
                                                                            tGTCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACgg                    >  1:1710916/1‑100 (MQ=255)
                                                                              tCGTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCg                  <  1:1340118/100‑1 (MQ=255)
                                                                                gTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTa                >  1:1248247/1‑100 (MQ=255)
                                                                                gTTGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTa                >  2:362183/1‑100 (MQ=255)
                                                                                 ttGTACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTAc               <  1:2658921/100‑1 (MQ=255)
                                                                                    tACAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCt            <  1:945464/100‑1 (MQ=255)
                                                                                      cAGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTa          >  1:2416642/1‑100 (MQ=255)
                                                                                       aGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTAc         <  2:1813823/100‑1 (MQ=255)
                                                                                       aGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTAc         <  1:2240755/100‑1 (MQ=255)
                                                                                       aGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTAc         <  1:1752401/100‑1 (MQ=255)
                                                                                       aGACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTAc         <  1:1479757/100‑1 (MQ=255)
                                                                                        naCGGCGGACCATTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGAGATGACCACATGGCCGTTACGGCGTACGCTTACt        >  2:1601223/2‑100 (MQ=255)
                                                                                        gACGGCGGACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACt        <  1:287979/100‑1 (MQ=255)
                                                                                          cggcggACCTTTTTCACCTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACAACATGGCCGTTACGGCGTACGCTTa          <  2:2553675/97‑1 (MQ=255)
                                                                                            gcggACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAg    <  1:2344555/100‑1 (MQ=255)
                                                                                             cggACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGt   >  2:2923698/1‑100 (MQ=255)
                                                                                             cggACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGt   <  1:909199/100‑1 (MQ=255)
                                                                                             cggACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGt   >  1:1255747/1‑100 (MQ=255)
                                                                                              ggACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACtatact   >  2:2032161/1‑97 (MQ=255)
                                                                                              ggACCTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGtt  >  1:2336325/1‑100 (MQ=255)
                                                                                                   |                                                                                               
ATTTCCAGTTTACGTTCAAACAGTTCGAACAGCGTAAAAGCATCCGCAGTACCGCCCGCAAAGCCCGCGATGACTTTGTCGTTGTACAGACGGCGGACCTTTTTCACGTTGCCTTTCATTACGGTATTGCCCAACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTT  >  NC_000913/4122088‑4122282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: