Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 751,952 (T)7→6 coding (218/2448 nt) ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913751,9460T.93.4% 469.9 / 26.9 121coding (224/2448 nt)ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base T (6/2);  new base . (50/63);  total (56/65)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 134 total aligned reads displayed.

ATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCC  >  NC_000913/751847‑752041
                                                                                                   |                                                                                               
   cacCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCtttt                                                                                              >  1:2269712/1‑100 (MQ=255)
    acCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCttttt                                                                                             <  2:1637193/100‑1 (MQ=255)
     ccGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCtttttt                                                                                            <  1:2798714/100‑1 (MQ=255)
     ccGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCtttttt                                                                                            >  2:1784707/1‑100 (MQ=255)
      cGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCttttttg                                                                                           <  1:373036/100‑2 (MQ=255)
      cGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCttttttg                                                                                           <  2:1149662/100‑2 (MQ=255)
      cGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCttttttg                                                                                           <  1:1946762/100‑2 (MQ=255)
           tatctGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGt                                                                                     >  2:558861/1‑100 (MQ=255)
            atctGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGtt                                                                                    >  2:755647/1‑100 (MQ=255)
            atctGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGtt                                                                                    <  1:2217273/100‑1 (MQ=255)
            atctGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGtt                                                                                    >  2:2855639/1‑100 (MQ=255)
            atctGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGtt                                                                                    <  1:428505/100‑1 (MQ=255)
               tgtgGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAAt                                                                                 >  2:823697/1‑100 (MQ=255)
                   gCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGGCAGTTAAtttt                                                                              <  2:735565/99‑1 (MQ=255)
                    cAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAAtttttt                                                                            >  1:452904/1‑100 (MQ=255)
                     aaGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGTATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTg                                                                           <  2:1153152/100‑1 (MQ=255)
                      aGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGc                                                                          <  1:150686/100‑1 (MQ=255)
                       ggACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGcc                                                                         <  2:39870/100‑1 (MQ=255)
                        gACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGccc                                                                        <  2:1196755/100‑1 (MQ=255)
                        gACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGccc                                                                        <  1:578077/100‑1 (MQ=255)
                        gACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGccc                                                                        <  1:1422446/100‑1 (MQ=255)
                          ctaaCGGATAGCTGGTTTTAAACCAAATTTATCTACCAGTGAATCATTCATGCATGTAATGTTTCTGTCACCCTTTTTTGCCATTTAATTTTTTGc                                                                          <  2:1211016/93‑1 (MQ=255)
                          cTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCAt                                                                      >  2:1435539/1‑100 (MQ=255)
                           tGACGGATATCTGGTTTTACACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCa                                                                       <  1:1202317/98‑1 (MQ=255)
                           tGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCAtt                                                                     <  2:2872988/100‑1 (MQ=255)
                           tGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCAtt                                                                     <  1:867131/100‑1 (MQ=255)
                             aCGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTAc                                                                   <  1:602224/100‑1 (MQ=255)
                             aCGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTAc                                                                   <  2:1380351/100‑1 (MQ=255)
                             aCGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTAc                                                                   <  1:122465/100‑1 (MQ=255)
                                 atatCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGAt                                                               <  1:2023142/100‑1 (MQ=255)
                                  tatCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGAt                                                               >  1:1803159/1‑99 (MQ=255)
                                    tCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGTTCATTACTGAtttt                                                            >  2:2159603/1‑100 (MQ=255)
                                    tCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGAtttt                                                            >  1:2269297/1‑100 (MQ=255)
                                     cTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttg                                                           >  1:2569478/1‑100 (MQ=255)
                                     atctttttAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGAttt                                                             <  2:2986839/94‑1 (MQ=255)
                                      tGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgt                                                          <  2:859828/100‑1 (MQ=255)
                                      tGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgt                                                          >  1:1676859/1‑100 (MQ=255)
                                       ggTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTTTTTTTTATTTTTTGCCCATTACTGATTttgtt                                                         >  2:1333617/1‑100 (MQ=255)
                                       ggTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgtt                                                         <  1:1384165/100‑1 (MQ=255)
                                       ggTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgtt                                                         <  1:592116/100‑1 (MQ=255)
                                       ggTTTAAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGTCAGTTAATTTTTTGCCCATTACTGATTttgtt                                                         >  2:1584215/1‑100 (MQ=255)
                                        gTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgttg                                                        <  1:354255/100‑1 (MQ=255)
                                        gTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgttg                                                        <  2:2828408/100‑1 (MQ=255)
                                        gTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgttg                                                        <  2:452900/100‑1 (MQ=255)
                                        gTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgttg                                                        >  1:903786/1‑100 (MQ=255)
                                         ttttAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTtgttgt                                                       >  2:2458822/1‑100 (MQ=255)
                                          tttAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTgttgtt                                                      >  1:2071671/1‑100 (MQ=255)
                                          tttAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTgttgtt                                                      >  1:2001711/1‑100 (MQ=255)
                                          tttAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTgttgtt                                                      >  1:342687/1‑100 (MQ=255)
                                           ttAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTa                                                     >  1:2313126/1‑100 (MQ=255)
                                           ttAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTa                                                     >  2:2721161/1‑100 (MQ=255)
                                            tgaaCCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTAt                                                    >  1:2808549/3‑100 (MQ=255)
                                            tAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGATTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTAt                                                    <  2:1936694/100‑1 (MQ=255)
                                             aaaCCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTAtt                                                   >  2:2319876/1‑100 (MQ=255)
                                             aaaCCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTAtt                                                   >  2:2709221/1‑100 (MQ=255)
                                             aaaCCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTAtt                                                   <  2:1228776/100‑1 (MQ=255)
                                             aaaCCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTAtt                                                   <  2:2269692/100‑1 (MQ=255)
                                                ccAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCa                                                 >  2:2138767/1‑100 (MQ=255)
                                                ccAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCAc                                                <  1:823704/100‑1 (MQ=255)
                                                   aaTTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGTCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGc                                             <  2:1654340/100‑1 (MQ=255)
                                                   aaTTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGc                                             <  2:447688/100‑1 (MQ=255)
                                                   aaTTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGc                                             <  1:617930/100‑1 (MQ=255)
                                                     tttATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCa                                            <  1:1647806/100‑1 (MQ=255)
                                                      ttATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAAc                                          <  2:1937747/100‑1 (MQ=255)
                                                          cGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTa                                      >  2:2111556/1‑100 (MQ=255)
                                                           gACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTaa                                     >  2:807217/1‑100 (MQ=255)
                                                           gACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTaa                                     >  1:1390838/1‑100 (MQ=255)
                                                            aCCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAAc                                    <  2:552359/100‑1 (MQ=255)
                                                             ccAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTTTTTTTTTTTTTTTGCCCATTACTGATTTTGTTGTTATTCTTCGCAACGCTAACa                                   >  2:1117857/1‑100 (MQ=255)
                                                               aGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACaa                                  >  1:1734287/1‑99 (MQ=255)
                                                                gTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACaaaa                                >  1:1662386/1‑100 (MQ=255)
                                                                  gAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAata                              <  2:1951876/100‑1 (MQ=255)
                                                                  gAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAata                              <  1:1784722/100‑1 (MQ=255)
                                                                  gAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAata                              <  1:755654/100‑1 (MQ=255)
                                                                    aTCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATAtt                            >  1:661841/1‑100 (MQ=255)
                                                                     tCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTc                           <  1:2824521/100‑1 (MQ=255)
                                                                     tCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTc                           <  1:2737232/100‑1 (MQ=255)
                                                                      cATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTTTTTTTTTTTTTTTGCACATTACTGATTTTGTTGTTATTCATCGTAACGCTAACAAAATAtt                            >  2:1882989/1‑98 (MQ=255)
                                                                      cATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCTTGTTATTTTTTTGCCCATTACTGACTTTGTTGTTATTCACTGCACTGCTAACaaa                                 >  2:1508402/1‑93 (MQ=255)
                                                                      cATTGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCa                          >  2:26940/1‑100 (MQ=255)
                                                                       aTTGATGCATGGAATGGTTTTGTCACCCTTTTTTTTTTTTTTTTTTTTTACCCATTACTGATTTTGTTGTTATTTATTGTAATGCTAACAAAATAtt                            >  2:1635939/1‑97 (MQ=255)
                                                                        ttGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCTcc                        <  1:760514/100‑1 (MQ=255)
                                                                        ttGATGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCAcc                        >  1:1066320/1‑100 (MQ=255)
                                                                           aTGCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCggg                     <  2:1339078/100‑1 (MQ=255)
                                                                             gCATGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAg                   <  2:1983957/100‑1 (MQ=255)
                                                                             gCATGGAATGGTTTTGTCACCCCTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAg                   <  1:521106/100‑1 (MQ=255)
                                                                               aTGGAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCa                 <  1:2319896/100‑1 (MQ=255)
                                                                                 ggAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAAt               >  2:1931825/1‑100 (MQ=255)
                                                                                  gAATGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATg              <  2:2569455/100‑1 (MQ=255)
                                                                                    aTGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGAc            >  2:892255/1‑100 (MQ=255)
                                                                                    aTGGTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGAc            >  2:329694/1‑100 (MQ=255)
                                                                                      ggTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTc          >  1:464225/1‑100 (MQ=255)
                                                                                       gTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTcc         <  1:2709245/100‑1 (MQ=255)
                                                                                       gTTTTGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTcc         >  1:399075/1‑100 (MQ=255)
                                                                                           tGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCtttt     <  2:342682/100‑1 (MQ=255)
                                                                                           tGTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCtttt     >  1:1525437/1‑100 (MQ=255)
                                                                                            gTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCttttt    <  1:321482/100‑1 (MQ=255)
                                                                                            gTCACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCttttt    >  1:1475655/1‑100 (MQ=255)
                                                                                              cACCCTTTTTTGCCAGTTAATTTTTTGCCCATTATTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGGCTCGTTTTTcc  <  2:1599241/100‑1 (MQ=255)
                                                                                              cACCCTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTcc  >  1:392008/1‑100 (MQ=255)
                                                                                                   |                                                                                               
ATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCC  >  NC_000913/751847‑752041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: