Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 758,290 Δ1 bp coding (602/717 nt) sdhB → succinate dehydrogenase, FeS subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913758,2890T.99.1% 474.3 / ‑2.6 106coding (601/717 nt)sdhBsuccinate dehydrogenase, FeS subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (51/54);  total (52/54)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 109 total aligned reads displayed.

AAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATG  >  NC_000913/758190‑758384
                                                                                                   |                                                                                               
aaGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTCTGAGTGATGCAt                                                                                                 <  1:1993033/100‑1 (MQ=255)
 aGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCAtc                                                                                                <  2:1101306/100‑2 (MQ=255)
  gTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCAtca                                                                                               >  1:633916/1‑98 (MQ=255)
     tATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCg                                                                                           <  2:1363927/100‑1 (MQ=255)
      aTCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGt                                                                                          <  2:2025416/100‑1 (MQ=255)
         ggCCCGGCAGGCTTGTTAGCTGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTAtt                                                                                       >  1:2850509/1‑100 (MQ=255)
         ggCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTAtt                                                                                       <  1:2314679/100‑1 (MQ=255)
         ggCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTAtt                                                                                       >  2:509363/1‑100 (MQ=255)
          gCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTc                                                                                      >  2:671544/1‑100 (MQ=255)
             cGGCAGGCTTGTTAACGGCATAACGCTTCCTGATTGATAGCCGTGATACCGAGACTGCCAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGc                                                                                   <  1:601205/100‑1 (MQ=255)
              ggCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCt                                                                                  >  1:1132554/1‑100 (MQ=255)
               tctggCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTg                                                                                 <  1:1020157/97‑1 (MQ=255)
               gCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTg                                                                                 <  2:2640091/100‑1 (MQ=255)
               gCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTg                                                                                 <  1:253440/100‑1 (MQ=255)
               gCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTg                                                                                 >  1:1079084/1‑100 (MQ=255)
                cAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGt                                                                                >  2:1725464/1‑100 (MQ=255)
                 aGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTc                                                                               <  1:1417801/100‑1 (MQ=255)
                  ggCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTgaga                                                                              >  2:1020147/1‑97 (MQ=255)
                  ggCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTca                                                                              >  2:1910801/1‑100 (MQ=255)
                  ggCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTca                                                                              >  2:429669/1‑100 (MQ=255)
                   gCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTcac                                                                             >  1:372318/1‑100 (MQ=255)
                    cTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTcaca                                                                            >  2:1110630/1‑100 (MQ=255)
                    cTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTcaca                                                                            <  1:1300508/100‑1 (MQ=255)
                     ttGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAg                                                                           <  1:2506935/100‑1 (MQ=255)
                      tGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGc                                                                          >  1:398689/1‑100 (MQ=255)
                        ttAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGcat                                                                        <  2:51714/100‑1 (MQ=255)
                         tAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGcaac                                                                       <  1:671551/100‑3 (MQ=255)
                          aGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGcatca                                                                      <  2:1104377/100‑1 (MQ=255)
                           gCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGcatcat                                                                     <  2:586250/100‑1 (MQ=255)
                            cGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATg                                                                    >  2:512692/1‑100 (MQ=255)
                            cGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATg                                                                    <  2:2818032/100‑1 (MQ=255)
                            cGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATg                                                                    <  2:802996/100‑1 (MQ=255)
                             ggCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGa                                                                   <  1:2448938/100‑1 (MQ=255)
                             ggCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGa                                                                   <  1:1718230/100‑1 (MQ=255)
                             ggCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGa                                                                   <  2:2924520/100‑1 (MQ=255)
                             ggCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGa                                                                   <  1:1864520/100‑1 (MQ=255)
                               cATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAAc                                                                 <  2:1079074/100‑1 (MQ=255)
                                 tatCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTg                                                               <  1:2688540/100‑1 (MQ=255)
                                 tatCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTg                                                               >  1:1617289/1‑100 (MQ=255)
                                 tatCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTg                                                               <  2:2543693/100‑1 (MQ=255)
                                    cGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGAGTATTCCGCTGTCACAGCACCATGAACTGCGt                                                            >  2:797797/1‑100 (MQ=255)
                                     gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCCCAGCATCATGAACTGCggc                                                           >  1:3032251/1‑98 (MQ=255)
                                     gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTc                                                           >  1:880596/1‑100 (MQ=255)
                                     gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTGTGAGTGATGCATCAGCGTATTCCGCTGTCACGGAAACATGAACAGCGt                                                            >  2:3028189/1‑99 (MQ=255)
                                     gTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTATGAGAGATGTATGAGCGTATTCCACTGTCACAGCATCATGAACTGCg                                                             >  2:1126370/1‑98 (MQ=255)
                                       ttCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAg                                                         <  1:242968/100‑1 (MQ=255)
                                         ccTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg                                                       >  1:1063597/1‑100 (MQ=255)
                                         ccTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAgtg                                                       <  1:1739318/100‑1 (MQ=255)
                                             attgatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATg                                                   <  2:1485160/100‑1 (MQ=255)
                                                gatAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTcc                                                >  1:1450964/1‑100 (MQ=255)
                                                 atAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCg                                               <  1:1651241/100‑1 (MQ=255)
                                                 atAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCg                                               <  1:347843/100‑1 (MQ=255)
                                                   aGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGaa                                             >  1:172932/1‑100 (MQ=255)
                                                    gCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAg                                            <  2:2033648/100‑1 (MQ=255)
                                                    gCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAg                                            <  1:702715/100‑1 (MQ=255)
                                                      cGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAggg                                          >  2:1283981/1‑100 (MQ=255)
                                                        tGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCTTCATGAACTGCGTCAGTGTATGTCCGAAGGGGc                                        <  2:2001648/100‑1 (MQ=255)
                                                        tGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGc                                        <  1:782278/100‑1 (MQ=255)
                                                         gATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCt                                       >  2:1556050/1‑100 (MQ=255)
                                                           tACCGAGACTGACAGCCGCCTCGACGGTTTTATTGCTGCATCAGCGTATTCCGCTGTCACACCATCATGGACTGCGTCAGTGTATGTCCGAGGGGGCTGa                                     >  2:285548/1‑100 (MQ=255)
                                                            aCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGa                                     >  1:2027481/1‑99 (MQ=255)
                                                            aCCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGACAGTGTATGTCCGAAGGGGCTGaa                                    >  2:2562072/1‑100 (MQ=255)
                                                             ccGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACCGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAAc                                   >  2:2785397/1‑100 (MQ=255)
                                                             ccGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAAc                                   <  1:2523775/100‑1 (MQ=255)
                                                               gagaCTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAAccc                                 >  2:1310604/1‑100 (MQ=255)
                                                               gagaCTGACAGCCGCCGCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCAGCATGAACGGCGTCAGTGTATGTCCGAAGGGGCTGAAccc                                 <  2:1035636/100‑1 (MQ=255)
                                                                agaCTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAAccc                                 <  1:1767710/99‑1 (MQ=255)
                                                                   cTGACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAcg                             <  2:2995013/100‑1 (MQ=255)
                                                                     gACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAcgc                            >  1:2003725/1‑100 (MQ=255)
                                                                     gACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAcgcg                           <  2:172931/100‑1 (MQ=255)
                                                                     gACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAcgcg                           >  2:345073/1‑100 (MQ=255)
                                                                     gACAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAAATGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAcgc                            >  2:1252136/1‑99 (MQ=255)
                                                                       cAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAcgcgcg                         <  2:184309/100‑1 (MQ=255)
                                                                       cAGCCGCCTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAcgcgcg                         >  2:2665249/1‑100 (MQ=255)
                                                                         gccgccTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGcc                       >  1:1550534/1‑100 (MQ=255)
                                                                         gccgccTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGcc                       >  2:1079413/1‑100 (MQ=255)
                                                                         gccgccTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGcc                       <  1:947132/100‑1 (MQ=255)
                                                                            gacTCGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATc                    >  1:2058131/3‑100 (MQ=255)
                                                                                cGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGcc                <  2:855798/100‑1 (MQ=255)
                                                                                cGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGcc                >  1:1294468/1‑100 (MQ=255)
                                                                                cGACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGc                 >  2:1367389/1‑99 (MQ=255)
                                                                                 gACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAGCTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGcc                <  2:856760/99‑1 (MQ=255)
                                                                                 gACGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCa               >  1:1529654/1‑100 (MQ=255)
                                                                                 gACGGTTGGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGcc                <  2:1907306/99‑1 (MQ=255)
                                                                                   tcttttGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCata             <  1:2808181/97‑1 (MQ=255)
                                                                                   cGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCata             <  2:2850483/100‑1 (MQ=255)
                                                                                   cGGTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCata             >  2:1599689/1‑100 (MQ=255)
                                                                                    ggTTTGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCatat            >  2:746172/1‑100 (MQ=255)
                                                                                      tttGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCataaga          >  2:2808156/1‑97 (MQ=255)
                                                                                      tttGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCa          <  1:869438/100‑1 (MQ=255)
                                                                                      tttGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCa          <  1:1725479/100‑1 (MQ=255)
                                                                                       ttGAGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCa          >  2:1913708/1‑99 (MQ=255)
                                                                                          tgTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTc      <  2:2031366/99‑1 (MQ=255)
                                                                                          aGTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTc      <  1:2289434/100‑1 (MQ=255)
                                                                                           gTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTca     >  1:2031384/1‑99 (MQ=255)
                                                                                           gTGATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCg     >  1:705426/1‑100 (MQ=255)
                                                                                             gATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGAt   >  2:1062827/1‑100 (MQ=255)
                                                                                             gATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTAGAt   >  2:2052158/1‑100 (MQ=255)
                                                                                             gATGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGGCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGAt   >  1:2808000/1‑100 (MQ=255)
                                                                                              aTGCATCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGAtg  <  1:512698/100‑1 (MQ=255)
                                                                                                   |                                                                                               
AAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATG  >  NC_000913/758190‑758384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: